Name: Lacticaseibacillus rhamnosus DSM 20021
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6427

Type strain:

Species: Lacticaseibacillus rhamnosus

Culture col. no.: DSM 20021, ATCC 7469, CCM 1825, NCDO 243, NCIB 6375, WDCM 00101, CCUG 21452, NCTC 6375

Strain history: <- ATCC <- F.M. Strong

NCBI tax ID(s): 1423797 (strain), 47715 (species)

Section

Lacticaseibacillus rhamnosus DSM 20021 is an anaerobe, mesophilic bacterium of the family Lactobacillaceae.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	23-11-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Firmicutes
	Class	Bacilli
	Order	Lactobacillales
	Family	Lactobacillaceae
	Genus	Lacticaseibacillus
	Species	Lacticaseibacillus rhamnosus
	Full Scientific Name (PNU)	Lacticaseibacillus rhamnosus (Hansen 1968) Zheng et al. 2020

	Synonym	Lactobacillus casei subsp. rhamnosus
	Synonym	Lactobacillus rhamnosus

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8433]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8433]

Culture medium growth

[Ref.: #8433]

Culture medium link

Medium recipe at BacMedia

[Ref.: #8433]

Culture medium composition

expand / minimize

[Ref.: #8433]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #8433]

Culture medium growth

[Ref.: #8433]

Culture medium link

Medium recipe at BacMedia

[Ref.: #8433]

Culture medium composition

expand / minimize

[Ref.: #8433]

Culture medium

MRS MEDIUM (DSMZ Medium 11), microaerophilic

[Ref.: #8433]

Culture medium growth

[Ref.: #8433]

Culture medium link

Medium recipe at BacMedia

[Ref.: #8433]

Culture medium composition

expand / minimize

[Ref.: #41838]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #41838]

Culture medium growth

[Ref.: #41838]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8433]	growth	37 °C
[Ref.: #41838]	growth	37 °C
[Ref.: #47413]	growth	37 °C

[Ref.: #8433]	Temperature range	mesophilic
[Ref.: #41838]	Temperature range	mesophilic
[Ref.: #47413]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #47413]	Oxygen tolerance	anaerobe
[Ref.: #47413]	Oxygen tolerance	microaerophile

[Ref.: #8433]	Murein short key	A11.31
[Ref.: #8433]	Murein types	A4alpha L-Lys-D-Asp

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8433]	6198	-	-	-	-	-	+	-	-	-	-	-	+	+	+	+	+	-	-	+	+	-	+	+	+	+	+	+	+	-	+	-	-	+	-	+	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-
[Ref.: #8433]	6169	-	-	-	-	-	+	-	-	-	-	+	+	+	+	+	+	-	-	+	+	-	+	+	+	+	+	+	+	+/-	+	-	-	+	-	+	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-
[Ref.: #8433]	5739	-	-	-	-	-	+	-	-	-	-	+	+	+	+	+	+	-	-	+	+	-	+	+	+	+	+	+	+	+	+	-	-	+	-	+	-	-	-	-	+	+	-	+	-	-	-	-	-	-	-
[Ref.: #8433]	5738	-	-	-	-	-	+	-	-	-	-	+	+	+	+	+	+	-	-	+	+	-	+	+	+	+	+	+	+	+	+	-	+	+	-	+	-	-	-	-	-	+	-	+	-	-	-	-	+/-	-	-
[Ref.: #8433]	5737	-	-	-	-	-	+	-	-	-	-	+	+	+	+	+	+	-	-	+	+	-	+	+	+	+	+	+	+	+	+	-	-	+	-	+	-	-	-	-	+	+	-	+	-	-	-	-	+/-	-	-

API® ID32E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Ornithine decarboxylaseODC	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Urease/urea hydrolysisURE	Acid from L-arabitolLARL	Acid from D-galacturonic acidGAT	Acid from 5-Ketogluconate5KG	LipaseLIP	Phenol red (Acidification)RP	beta-Glucosidasebeta GLU	Acid from D-mannitolMAN	Acid from maltoseMAL	Acid from D-adonitolADO	Acid from palatinosePLE	beta-Glucuronidasebeta GUR	MalonateMNT	Indole productionIND	N-Acetyl-beta-Glucosaminidasebeta NAG	beta-Galactosidasebeta GAL	Acid from D-glucoseGLU	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	alpha-Glucosidasealpha GLU	alpha-Galactosidasealpha GAL	Acid from trehaloseTRE	Acid from L-rhamnoseRHA	Acid from inositolINO	Acid from cellobioseCEL	Acid from sorbitolSOR	alpha-MaltosidasealphaMAL	L-aspartic acid arylamidaseAspA
[Ref.: #47413]	855	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #47413]	845	-	-	+	+	-	+	+	-	+	-	-	-	not determinedn.d.	+	-	+	+	+	+	-	-

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	gluconeogenesis	100	8 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	100	8 of 8
[Ref.: #66794]	acetate fermentation	100	4 of 4
[Ref.: #66794]	ribulose monophosphate pathway	100	2 of 2
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	ceramide biosynthesis	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	acetoin degradation	100	3 of 3
[Ref.: #66794]	palmitate biosynthesis	90.91	20 of 22
[Ref.: #66794]	pentose phosphate pathway	90.91	10 of 11
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	peptidoglycan biosynthesis	86.67	13 of 15
[Ref.: #66794]	vitamin B1 metabolism	84.62	11 of 13
[Ref.: #66794]	degradation of sugar alcohols	81.25	13 of 16
[Ref.: #66794]	starch degradation	80	8 of 10
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	pyrimidine metabolism	75.56	34 of 45
[Ref.: #66794]	purine metabolism	75.53	71 of 94
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	photosynthesis	71.43	10 of 14
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	myo-inositol biosynthesis	70	7 of 10
[Ref.: #66794]	phenylalanine metabolism	69.23	9 of 13
[Ref.: #66794]	methionine metabolism	69.23	18 of 26
[Ref.: #66794]	glutamate and glutamine metabolism	67.86	19 of 28
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	valine metabolism	66.67	6 of 9
[Ref.: #66794]	degradation of hexoses	66.67	12 of 18
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	d-xylose degradation	63.64	7 of 11
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	dTDPLrhamnose biosynthesis	62.5	5 of 8
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	3-chlorocatechol degradation	60	3 of 5
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	Entner Doudoroff pathway	60	6 of 10
[Ref.: #66794]	oxidative phosphorylation	59.34	54 of 91
[Ref.: #66794]	non-pathway related	57.89	22 of 38
[Ref.: #66794]	degradation of pentoses	57.14	16 of 28
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	CO2 fixation in Crenarchaeota	55.56	5 of 9
[Ref.: #66794]	serine metabolism	55.56	5 of 9
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	metabolism of disaccharids	54.55	6 of 11
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	degradation of sugar acids	52	13 of 25
[Ref.: #66794]	alanine metabolism	51.72	15 of 29
[Ref.: #66794]	histidine metabolism	51.72	15 of 29
[Ref.: #66794]	cyclohexanol degradation	50	2 of 4
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	50	4 of 8
[Ref.: #66794]	tryptophan metabolism	50	19 of 38
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	isoleucine metabolism	50	4 of 8
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	tetrahydrofolate metabolism	50	7 of 14
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	glutathione metabolism	50	7 of 14
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	reductive acetyl coenzyme A pathway	42.86	3 of 7
[Ref.: #66794]	mevalonate metabolism	42.86	3 of 7
[Ref.: #66794]	isoprenoid biosynthesis	42.31	11 of 26
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	ascorbate metabolism	40.91	9 of 22
[Ref.: #66794]	lysine metabolism	40.48	17 of 42
[Ref.: #66794]	ethylmalonyl-CoA pathway	40	2 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	cellulose degradation	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	flavin biosynthesis	40	6 of 15
[Ref.: #66794]	vitamin K metabolism	40	2 of 5
[Ref.: #66794]	lipid metabolism	38.71	12 of 31
[Ref.: #66794]	citric acid cycle	35.71	5 of 14
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	chorismate metabolism	33.33	3 of 9
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	leucine metabolism	30.77	4 of 13
[Ref.: #66794]	urea cycle	30.77	4 of 13
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	propionate fermentation	30	3 of 10
[Ref.: #66794]	arginine metabolism	29.17	7 of 24
[Ref.: #66794]	vitamin B6 metabolism	27.27	3 of 11
[Ref.: #66794]	polyamine pathway	26.09	6 of 23
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	phenol degradation	25	5 of 20
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	vitamin B12 metabolism	23.53	8 of 34
[Ref.: #66794]	sulfate reduction	23.08	3 of 13
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
[Ref.: #66794]	tyrosine metabolism	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8433]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Lactobacillus rhamnosus gene for 16S rRNA, partial sequence, strain: YIT 0105 (= ATCC 7469)	AB008211	1540	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus gene for 16S ribosomal RNA, partial sequence, strain: ATCC 7469, clone: BF	AB759524	722	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus ATCC 7469a 16S ribosomal RNA gene, partial sequence	AF429476	507	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus ATCC 7469 16S ribosomal RNA gene, partial sequence	AF429485	507	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus strain ATCC 7469a 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF429563	537	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus strain ATCC 7469 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF429572	537	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus strain ATCC 7469 16S ribosomal RNA gene, partial sequence	JQ580982	969	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus 16S-23S rDNA intergenic spacer region	AF121201	218	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus strain DSM 20021 16S ribosomal RNA gene, partial sequence	HQ012008	936	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus casei 16S ribosomal RNA	M58815	1514	ENA	1582 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus genes for 16S rRNA, 16S-23S internal transcribed spacer, tRNA-Ile, tRNA-Ala, 23S rRNA, partial and complete sequence, strain:JCM 1136	AB237512	578	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus genes for 16S rRNA, 16S-23S internal transcribed spacer, 23S rRNA, partial sequence, strain:JCM 1136	AB237513	365	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus gene for 16S rRNA, partial sequence, strain: JCM 1136	AB289276	616	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus 16S ribosomal RNA, partial sequence; 16S/23S intergenic spacer region, complete sequence; and 23S ribosomal RNA partial sequence	AF182730	686	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus gene for 16S rRNA	D16552	1521	ENA	47715 tax ID	*
[Ref.: #20218]	Lactobacillus rhamnosus gene for 16S rRNA, partial sequence, strain: NBRC 3425	AB626049	1495	ENA	47715 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Lactobacillus rhamnosus NCTC13764	GCA_900636965	complete	GenBank	47715 tax ID	*
[Ref.: #66792]	Lactobacillus rhamnosus BFE5264	GCA_001988935	complete	GenBank	47715 tax ID	*
[Ref.: #66792]	Lactobacillus rhamnosus 4B15	GCA_002158925	complete	GenBank	47715 tax ID	*
[Ref.: #66792]	Lactobacillus rhamnosus BPL5	GCA_900070175	complete	GenBank	47715 tax ID	*
[Ref.: #66792]	Lactobacillus rhamnosus hsryfm 1301	GCA_008727835	complete	GenBank	47715 tax ID	*
[Ref.: #66792]	Lactobacillus rhamnosus MGYG-HGUT-01293	GCA_902381635	complete	GenBank	47715 tax ID	*

[Ref.: #8433]

GC-content

47.0 mol%

Availability in culture collections External links

[Ref.: #8433]

Culture collection no.

DSM 20021, ATCC 7469, CCM 1825, NCDO 243, NCIB 6375, WDCM 00101, CCUG 21452, NCTC 6375

[Ref.: #20218]

Associated Passport(s) in StrainInfo

14071, 14065, 94469, 94470, 866216

References

#8433

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20021

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41838 ; Curators of the CIP;
#47413 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 21452

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 )

* These References are textmined

Change proposal

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