Lactobacillus amylovorus subsp. amylovorus (DSM 20552, CIP 104566, H 27a)

Name: Lactobacillus amylovorus subsp. amylovorus (DSM 20552, CIP 104566, H 27a)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6415

Type strain

Strain Designation H 27a

Culture col. no. DSM 20552 CIP 104566 H 27a LMG 18186 KCTC 3170 1 more

NCBI tax ID(s) 3378537

Special Collections DSMZ JCM KCTC CIP

History <- E. Lauer <- G. Reuter, H 27a (Lactobacillus acidophilus) DSM 20552 <-- E. Lauer <-- G. Reuter H27a. CIP <- 1995, DSM <- E. Lauer <- G. Reuter: strain H27a, Lactobacillus acidophilus

Links

Lactobacillus amylovorus subsp. amylovorus H 27a is an anaerobe, mesophilic, Gram-positive prokaryote that produces lactate and was isolated from intestine of adult.

lactate production Gram-positive rod-shaped anaerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 11434

LMG 18186,CIP 104566,DSM 20552,JCM 2125,KCTC 3170

@ref 20215

Last LPSN update 2026-02-09 11:19:12

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lactobacillus

Species Lactobacillus amylovorus subsp. amylovorus

Full scientific name Lactobacillus amylovorus subsp. amylovorus (Nakamura 1981) Yamane et al. 2024

BacDive ID	Other strains from **Lactobacillus amylovorus subsp. amylovorus** (3)	Type strain
6413	L. amylovorus subsp. amylovorus B-4540, DSM 20531, ATCC 33620, NRRL B-4540, ... (type strain)
6414	L. amylovorus subsp. amylovorus B-4542, CCUG 27202, DSM 20532, ATCC 33622, ...
158985	L. amylovorus subsp. amylovorus Bifido-178-WT-3C, DSM 107288

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
116365	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8902	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
39048	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
116365	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8902	positive	growth	37	mesophilic
39048	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic
116365	positive	growth	30-45
116365	negative	growth	15

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
8902	anaerobe
8902	microaerophile
116365	facultative anaerobe

Compound production

8902

CompoundDL lactic acid

Murein

@ref	Murein short key	Type
8902	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
116365	17632 ChEBI	nitrate	-	reduction
116365	17632 ChEBI	nitrate	+	respiration
116365	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
116365	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
116365	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
116365	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
116365	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116365	ornithine decarboxylase	-	4.1.1.17
116365	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116365	-	-	+	-	-	+	-	+	-	-	+	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8902	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	+	-	+	+	+	+	+	-	+	+	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-
116365	not determinedn.d.	-	-	-	-	-	-	-	-	-	+/-	+	+/-	+	-	-	-	-	-	-	-	-	+	+/-	+/-	-	+	+	+	+	+/-	+	+	-	-	+/-	+/-	-	-	+	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host Body-Site	#Gastrointestinal tract

Isolation

@ref	Sample type
8902	intestine of adult
67770	Intestine of adult
116365	Human, Intestine

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8902	1	Risk group (German classification) According to TRBA 466
116365	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	Lactobacillus amylovorus gene for 16S rRNA, partial sequence, strain: JCM 2125.	AB289034	682		1604

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Lactic Acid Bacteria as Potential Biocontrol Agents for Fusarium Head Blight Disease of Spring Barley.	Byrne MB, Thapa G, Doohan FM, Burke JI.	Front Microbiol	10.3389/fmicb.2022.912632	2022
Metabolism	In Vitro Framework to Assess the Anti-Helicobacter pylori Potential of Lactic Acid Bacteria Secretions as Alternatives to Antibiotics.	Whiteside SA, Mohiuddin MM, Shlimon S, Chahal J, MacPherson CW, Jass J, Tompkins TA, Creuzenet C.	Int J Mol Sci	10.3390/ijms22115650	2021
Enzymology	Quantitative real-time PCR analysis of fecal Lactobacillus species in infants receiving a prebiotic infant formula.	Haarman M, Knol J.	Appl Environ Microbiol	10.1128/aem.72.4.2359-2365.2006	2006
Enzymology	The abundance of Zoogloea ramigera in sewage treatment plants.	Rossello-Mora RA, Wagner M, Amann R, Schleifer KH.	Appl Environ Microbiol	10.1128/aem.61.2.702-707.1995	1995

References

#8902	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20552
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#39048	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#116365	Collection of Institut Pasteur ; Curators of the CIP; CIP 104566
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactobacillus amylovorus subsp. amylovorus (DSM 20552, CIP 104566, H 27a)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Murein

Metabolite utilization

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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