Name: Roseburia inulinivorans A2-194
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6369

Type strain:

Species: Roseburia inulinivorans

Strain Designation: A2-194

Culture col. no.: DSM 16841, CIP 109405, JCM 17584, NCIMB 14030

Strain history: DSM 16841 <-- S. Duncan A2-194 <-- A. Barcenilla.

NCBI tax ID(s): 622312 (strain), 360807 (species)

SI-ID 294497

NCIMB 14030, DSM 16841, CIP 109405

Other strains from Roseburia inulinivorans

R. inulinivorans H1-37, H1_37, DSM 108070
R. inulinivorans JCM 31260

Section

Roseburia inulinivorans A2-194 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human faecal sample.

Gram-positive
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Lachnospiraceae*
	Genus	*Roseburia*
	Species	**Roseburia inulinivorans**
	Full Scientific Name (LPSN)	Roseburia inulinivorans Duncan et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6627]

Culture medium

ACETOMICROBIUM FAECALIS MEDIUM (DSMZ Medium 412)

[Ref.: #6627]

Culture medium growth

[Ref.: #6627]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6627]

Culture medium composition

expand / minimize

[Ref.: #6627]

Culture medium

YCFA-MEDIUM (MODIFIED) (DSMZ Medium 1611)

[Ref.: #6627]

Culture medium growth

[Ref.: #6627]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #6627]			growth	37 °C
[Ref.: #67770]			growth	37 °C

[Ref.: #6627]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6627]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.589

[Ref.: #69480]

Ability of spore formation

no Confidence in %96.362

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #6627]	12962	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+/-	-	-	-
[Ref.: #6627]	12638	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+/-	+/-	-	-
[Ref.: #6627]	12637	-	+/-	+	+	-	-	+	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	-	+	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #6627]	12636	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6627]	Sample type/isolated from	human faecal sample
[Ref.: #6627]	Geographic location (country and/or sea, region)	Scotland, Aberdeen
[Ref.: #6627]	Country	United Kingdom
[Ref.: #6627]	Country ISO 3 Code	GBR
[Ref.: #6627]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Human feces in Aberdeen
[Ref.: #67770]	Geographic location (country and/or sea, region)	Scotland
[Ref.: #67770]	Country	United Kingdom
[Ref.: #67770]	Country ISO 3 Code	GBR
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6627]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Roseburia inulinivorans gene for 16S ribosomal RNA, partial sequence, strain: JCM 17584	AB661436	1494	ENA	360807 tax ID
[Ref.: #6627]	Roseburia inulinivorans 16S rRNA gene, type strain A2-194T	AJ270473	1454	ENA	622312 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Roseburia inulinivorans DSM 16841	GCA_000174195	contig	GenBank	622312 tax ID
[Ref.: #66792]	Roseburia inulinivorans DSM 16841 FDAARGOS_1587	GCA_020731525	contig	GenBank	622312 tax ID	*
[Ref.: #66792]	Roseburia inulinivorans DSM 16841	622312.4	wgs	PATRIC	622312 tax ID	*
[Ref.: #66792]	Roseburia inulinivorans DSM 16841	643886006	draft	JGI IMG/M	622312 tax ID	*

[Ref.: #6627]	GC-content	41.4 mol%
[Ref.: #67770]	GC-content	41.4 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Roseburia inulinivorans DSM 16841 PATRIC: 622312.4
[Ref.: #69481]	spore-forming	yes	60	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Roseburia inulinivorans DSM 16841 PATRIC: 622312.4
[Ref.: #69480]	gram-positive	yes	85.984	no
[Ref.: #69480]	anaerobic	yes	99.681	no
[Ref.: #69480]	halophile	no	87.136	no
[Ref.: #69480]	spore-forming	yes	64.587	no
[Ref.: #69480]	glucose-util	yes	89.942	no
[Ref.: #69480]	thermophile	no	99.076	no
[Ref.: #69480]	flagellated	no	61.57	no
[Ref.: #69480]	aerobic	no	99.33	yes
[Ref.: #69480]	motile	yes	82.01	no
[Ref.: #69480]	glucose-ferment	yes	81.8	no

Availability in culture collections External links

[Ref.: #6627]

Culture collection no.

DSM 16841, CIP 109405, JCM 17584, NCIMB 14030

[Ref.: #75842]

SI-ID 294497

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Heterologous gene expression in the human gut bacteria Eubacterium rectale and Roseburia inulinivorans by means of conjugative plasmids.	Sheridan PO, Martin JC, Minton NP, Flint HJ, O'Toole PW, Scott KP	Anaerobe	10.1016/j.anaerobe.2019.06.008	2019	*
Pathogenicity	Influence of H7N9 virus infection and associated treatment on human gut microbiota.	Qin N, Zheng B, Yao J, Guo L, Zuo J, Wu L, Zhou J, Liu L, Guo J, Ni S, Li A, Zhu Y, Liang W, Xiao Y, Ehrlich SD, Li L	Sci Rep	10.1038/srep14771	2015	*
Metabolism	Substrate-driven gene expression in Roseburia inulinivorans: importance of inducible enzymes in the utilization of inulin and starch.	Scott KP, Martin JC, Chassard C, Clerget M, Potrykus J, Campbell G, Mayer CD, Young P, Rucklidge G, Ramsay AG, Flint HJ	Proc Natl Acad Sci U S A	10.1073/pnas.1000091107	2010	*
Metabolism	In vitro kinetics of prebiotic inulin-type fructan fermentation by butyrate-producing colon bacteria: implementation of online gas chromatography for quantitative analysis of carbon dioxide and hydrogen gas production.	Falony G, Verschaeren A, De Bruycker F, De Preter V, Verbeke K, Leroy F, De Vuyst L	Appl Environ Microbiol	10.1128/AEM.00876-09	2009	*
Phylogeny	Transfer of conjugative elements from rumen and human Firmicutes bacteria to Roseburia inulinivorans.	Scott KP, Martin JC, Mrazek J, Flint HJ	Appl Environ Microbiol	10.1128/AEM.02807-07	2008	*
Phylogeny	Cell-associated alpha-amylases of butyrate-producing Firmicute bacteria from the human colon.	Ramsay AG, Scott KP, Martin JC, Rincon MT, Flint HJ	Microbiology (Reading)	10.1099/mic.0.29233-0	2006	*
Phylogeny	Proposal of Roseburia faecis sp. nov., Roseburia hominis sp. nov. and Roseburia inulinivorans sp. nov., based on isolates from human faeces.	Duncan SH, Aminov RI, Scott KP, Louis P, Stanton TB, Flint HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.64098-0	2006	*
Metabolism	Whole-genome transcription profiling reveals genes up-regulated by growth on fucose in the human gut bacterium "Roseburia inulinivorans".	Scott KP, Martin JC, Campbell G, Mayer CD, Flint HJ	J Bacteriol	10.1128/JB.00137-06	2006	*

References

#6627

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16841

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75842

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID294497.1 )

* These data were automatically processed and therefore are not curated

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Roseburia inulinivorans DSM 16841

Roseburia inulinivorans DSM 16841

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