Name: Roseburia intestinalis L1-82
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6366

Type strain:

Species: Roseburia intestinalis

Strain Designation: L1-82

Culture col. no.: DSM 14610, CIP 107878, JCM 17583, NCIMB 13810, VTT E-052785

Strain history: DSM 14610 <-- S. Duncan L1-82.

NCBI tax ID(s): 166486 (species)

SI-ID 100933

DSM 14610, NCIMB 13810, CIP107878, VTT E-052785

Other strains from Roseburia intestinalis

R. intestinalis JCM 31262

Section

Roseburia intestinalis L1-82 is a mesophilic bacterium that was isolated from infant faeces.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Lachnospiraceae*
	Genus	*Roseburia*
	Species	**Roseburia intestinalis**
	Full Scientific Name (LPSN)	Roseburia intestinalis Duncan et al. 2002

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5410]

Culture medium

YCFA-MEDIUM (MODIFIED) (DSMZ Medium 1611)

[Ref.: #5410]

Culture medium growth

[Ref.: #5410]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5410]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5410]			growth	37 °C
[Ref.: #67770]			growth	37 °C

[Ref.: #5410]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	+	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #5410]	12963	-	-	+	+	-	+	+	+	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #5410]	12608	-	+/-	+	+	-	+	+	+	-	+/-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #5410]	12607	-	-	+	+	-	+	+	+	-	+/-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #5410]	12606	-	-	+	+	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #5410]	12605	-	-	+	+	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #5410]	12604	-	-	+	+	-	+	+	+	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	+	-	-
[Ref.: #5410]	12603	-	-	+	+	-	+	+	+	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #5410]	12103	-	-	+	+	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5410]	Sample type/isolated from	infant faeces
[Ref.: #5410]	Geographic location (country and/or sea, region)	Scotland, Aberdeen
[Ref.: #5410]	Country	United Kingdom
[Ref.: #5410]	Country ISO 3 Code	GBR
[Ref.: #5410]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Healthy infant fecal samples
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Child

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HM007565 (>99% sequence identity) for Roseburia intestinalis subclade from Microbeatlas

Aquatic Samples	311
Soil Samples	217
Animal Samples	37648
Plant Samples	44
Total Samples	38220

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5410]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5410]	Roseburia intestinalis 16S rRNA gene, strain L1-82	AJ312385	1482	ENA	536231 tax ID
[Ref.: #67770]	Roseburia intestinalis gene for 16S ribosomal RNA, partial sequence, strain: JCM 17583	AB661435	1494	ENA	166486 tax ID
[Ref.: #20218]	Roseburia intestinalis strain DSM 14610 16S ribosomal RNA gene, partial sequence	HM007565	1329	ENA	166486 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Roseburia intestinalis L1-82	GCA_900537995	complete	GenBank	536231 tax ID	*
[Ref.: #66792]	Roseburia intestinalis L1-82	GCA_000156535	scaffold	GenBank	536231 tax ID	*
[Ref.: #66792]	Roseburia intestinalis L1-82	536231.5	wgs	PATRIC	536231 tax ID	*
[Ref.: #66792]	Roseburia intestinalis L1-82	536231.75	complete	PATRIC	536231 tax ID	*
[Ref.: #66792]	Roseburia intestinalis L1-82	2836830276	complete	JGI IMG/M	536231 tax ID	*
[Ref.: #66792]	Roseburia intestinalis L1-82	2562617159	draft	JGI IMG/M	536231 tax ID	*

[Ref.: #67770]

GC-content

31 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Roseburia intestinalis L1-82 L1-82 NCBI: GCA_900537995.1
[Ref.: #69480]	flagellated	no	63.636	no
[Ref.: #69480]	gram-positive	yes	83.673	no
[Ref.: #69480]	anaerobic	yes	99.701	no
[Ref.: #69480]	aerobic	no	99.172	no
[Ref.: #69480]	halophile	no	91.789	no
[Ref.: #69480]	spore-forming	yes	74.51	no
[Ref.: #69480]	thermophile	no	95.495	yes
[Ref.: #69480]	glucose-util	yes	90.792	no
[Ref.: #69480]	motile	yes	80.682	no
[Ref.: #69480]	glucose-ferment	yes	76.235	no

Availability in culture collections External links

[Ref.: #5410]

Culture collection no.

DSM 14610, CIP 107878, JCM 17583, NCIMB 13810, VTT E-052785

[Ref.: #75839]

SI-ID 100933

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Butyribacter intestini gen. nov., sp. nov., a butyric acid-producing bacterium of the family Lachnospiraceae isolated from human faeces, and reclassification of Acetivibrio ethanolgignens as Acetanaerobacter ethanolgignens gen. nov., comb. nov.	Zou Y, Xue W, Lin X, Lv M, Luo G, Dai Y, Sun H, Liu SW, Sun CH, Hu T, Xiao L	Syst Appl Microbiol	10.1016/j.syapm.2021.126201	2021	*
Enzymology	The enemy from within: a prophage of Roseburia intestinalis systematically turns lytic in the mouse gut, driving bacterial adaptation by CRISPR spacer acquisition.	Cornuault JK, Moncaut E, Loux V, Mathieu A, Sokol H, Petit MA, De Paepe M	ISME J	10.1038/s41396-019-0566-x	2019	*
Metabolism	Integrated culturing, modeling and transcriptomics uncovers complex interactions and emergent behavior in a three-species synthetic gut community.	D'hoe K, Vet S, Faust K, Moens F, Falony G, Gonze D, Llorens-Rico V, Gelens L, Danckaert J, De Vuyst L, Raes J	Elife	10.7554/eLife.37090	2018	*
Metabolism	In vitro kinetics of prebiotic inulin-type fructan fermentation by butyrate-producing colon bacteria: implementation of online gas chromatography for quantitative analysis of carbon dioxide and hydrogen gas production.	Falony G, Verschaeren A, De Bruycker F, De Preter V, Verbeke K, Leroy F, De Vuyst L	Appl Environ Microbiol	10.1128/AEM.00876-09	2009	*
Metabolism	Cross-feeding between Bifidobacterium longum BB536 and acetate-converting, butyrate-producing colon bacteria during growth on oligofructose.	Falony G, Vlachou A, Verbrugghe K, De Vuyst L	Appl Environ Microbiol	10.1128/AEM.01296-06	2006	*
Phylogeny	Proposal of Roseburia faecis sp. nov., Roseburia hominis sp. nov. and Roseburia inulinivorans sp. nov., based on isolates from human faeces.	Duncan SH, Aminov RI, Scott KP, Louis P, Stanton TB, Flint HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.64098-0	2006	*
Pathogenicity	Effects of alternative dietary substrates on competition between human colonic bacteria in an anaerobic fermentor system.	Duncan SH, Scott KP, Ramsay AG, Harmsen HJ, Welling GW, Stewart CS, Flint HJ	Appl Environ Microbiol	10.1128/AEM.69.2.1136-1142.2003	2003	*
Phylogeny	Roseburia intestinalis sp. nov., a novel saccharolytic, butyrate-producing bacterium from human faeces.	Duncan SH, Hold GL, Barcenilla A, Stewart CS, Flint HJ	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1615	2002	*
Metabolism	Peanuts as a nighttime snack enrich butyrate-producing bacteria compared to an isocaloric lower-fat higher-carbohydrate snack in adults with elevated fasting glucose: A randomized crossover trial.	Sapp PA, Kris-Etherton PM, Arnesen EA, Chen See JR, Lamendella R, Petersen KS	Clin Nutr	10.1016/j.clnu.2022.08.004	2022	*

References

#5410

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14610

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#75839

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100933.1 )

* These data were automatically processed and therefore are not curated

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Roseburia intestinalis L1-82 L1-82

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