Kineosporia aurantiaca (DSM 43858, ATCC 29727, IFO 13890)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	11340
  multimedia content:	DSM_43858.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43858.jpg
  caption:	Medium 214 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
11340	GYM+S MEDIUM (DSMZ Medium 214)		Medium recipe at MediaDive	Name: GYM+S MEDIUM (DSMZ Medium 214) Composition: Starch 20.0 g/l Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19969	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19969	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19969	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19969	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19969	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19969	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

Oxygen tolerance

Halophily

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
19969	22599 ChEBI	arabinose	+
19969	62968 ChEBI	cellulose	-
19969	28757 ChEBI	fructose	+
19969	17234 ChEBI	glucose	+
19969	29864 ChEBI	mannitol	+
19969	17268 ChEBI	myo-inositol	+
19969	16634 ChEBI	raffinose	+
19969	26546 ChEBI	rhamnose	+
19969	17992 ChEBI	sucrose	+
19969	18222 ChEBI	xylose	-

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence X87110 (>99% sequence identity) for Kineosporia aurantiaca from Microbeatlas

Aquatic Samples	68
Soil Samples	284
Animal Samples	152
Plant Samples	37
Total Samples	541

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM71902v1 assembly for Kineosporia aurantiaca JCM 3230 NRRL B-16913	contig	GCA_000719025	1306174.4	2772190730	1306174	37.76
66792	ASM131532v1 assembly for Kineosporia aurantiaca JCM 3230	contig	GCA_001315325	1306174.8	2734481982	1306174

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	K.aurantiaca 16S rRNA gene	X87110	1501		1306174
20218	Kineosporia aurantiaca gene for 16S ribosomal RNA, partial sequence	D86937	1469		1306174
20218	Kineosporia aurantiaca gene for 16S rRNA, partial sequence, strain:JCM3230	AB003931	1472		1306174
20218	Kineosporia aurantiaca strain NRRL B-16913 16S ribosomal RNA gene, partial sequence	AF095336	1513		49185

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Denitrification by actinomycetes and purification of dissimilatory nitrite reductase and azurin from Streptomyces thioluteus.	Shoun H, Kano M, Baba I, Takaya N, Matsuo M.	J Bacteriol	10.1128/jb.180.17.4413-4415.1998	1998
Phylogeny	Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed.	Smalla K, Wieland G, Buchner A, Zock A, Parzy J, Kaiser S, Roskot N, Heuer H, Berg G.	Appl Environ Microbiol	10.1128/aem.67.10.4742-4751.2001	2001
Enzymology	Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients.	Heuer H, Krsek M, Baker P, Smalla K, Wellington EM.	Appl Environ Microbiol	10.1128/aem.63.8.3233-3241.1997	1997
Enzymology	Effects of T4 lysozyme release from transgenic potato roots on bacterial rhizosphere communities are negligible relative to natural factors.	Heuer H, Kroppenstedt RM, Lottmann J, Berg G, Smalla K.	Appl Environ Microbiol	10.1128/aem.68.3.1325-1335.2002	2002
Proteome	Enhancing tandem mass spectrometry-based metabolite annotation with online chemical labeling.	Vitale GA, Xia SN, Duhrkop K, Zare Shahneh MR, Brotz-Oesterhelt H, Mast Y, Brungs C, Bocker S, Schmid R, Wang M, Hughes CC, Petras D.	Nat Commun	10.1038/s41467-025-61240-z	2025
	A rare peptide scaffold in kineomicins, the glycopeptide antibiotics produced by Actinokineospora auranticolor DSM 44650.	Yushchuk O, Berini F, Zhong L, Ruckert-Reed C, Bernasconi E, Bartolone L, Busche T, Kalinowski J, Sussmuth RD, Marinelli F.	Commun Chem	10.1038/s42004-025-01534-x	2025
	New Molecular Tools for Regulation and Improvement of A40926 Glycopeptide Antibiotic Production in Nonomuraea gerenzanensis ATCC 39727.	Yushchuk O, Andreo-Vidal A, Marcone GL, Bibb M, Marinelli F, Binda E.	Front Microbiol	10.3389/fmicb.2020.00008	2020
Enzymology	Partial characterization of chitinolytic enzymes from Streptomyces albidoflavus.	Broadway RM, Williams DL, Kain WC, Harman GE, Lorito M, Labeda DP.	Lett Appl Microbiol	10.1111/j.1472-765x.1995.tb00444.x	1995
	Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.	Lu J, He Q, Huang L, Cai X, Guo W, He J, Zhang L, Li A.	PLoS One	10.1371/journal.pone.0117690	2015
Phylogeny	Phylogenetic relationships in the family Streptomycetaceae using multi-locus sequence analysis.	Labeda DP, Dunlap CA, Rong X, Huang Y, Doroghazi JR, Ju KS, Metcalf WW.	Antonie Van Leeuwenhoek	10.1007/s10482-016-0824-0	2017
Genetics	Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages.	Klyczek KK, Bonilla JA, Jacobs-Sera D, Adair TL, Afram P, Allen KG, Archambault ML, Aziz RM, Bagnasco FG, Ball SL, Barrett NA, Benjamin RC, Blasi CJ, Borst K, Braun MA, Broomell H, Brown CB, Brynell ZS, Bue AB, Burke SO, Casazza W, Cautela JA, Chen K, Chimalakonda NS, Chudoff D, Connor JA, Cross TS, Curtis KN, Dahlke JA, Deaton BM, Degroote SJ, DeNigris DM, DeRuff KC, Dolan M, Dunbar D, Egan MS, Evans DR, Fahnestock AK, Farooq A, Finn G, Fratus CR, Gaffney BL, Garlena RA, Garrigan KE, Gibbon BC, Goedde MA, Guerrero Bustamante CA, Harrison M, Hartwell MC, Heckman EL, Huang J, Hughes LE, Hyduchak KM, Jacob AE, Kaku M, Karstens AW, Kenna MA, Khetarpal S, King RA, Kobokovich AL, Kolev H, Konde SA, Kriese E, Lamey ME, Lantz CN, Lapin JS, Lawson TO, Lee IY, Lee SM, Lee-Soety JY, Lehmann EM, London SC, Lopez AJ, Lynch KC, Mageeney CM, Martynyuk T, Mathew KJ, Mavrich TN, McDaniel CM, McDonald H, McManus CJ, Medrano JE, Mele FE, Menninger JE, Miller SN, Minick JE, Nabua CT, Napoli CK, Nkangabwa M, Oates EA, Ott CT, Pellerino SK, Pinamont WJ, Pirnie RT, Pizzorno MC, Plautz EJ, Pope WH, Pruett KM, Rickstrew G, Rimple PA, Rinehart CA, Robinson KM, Rose VA, Russell DA, Schick AM, Schlossman J, Schneider VM, Sells CA, Sieker JW, Silva MP, Silvi MM, Simon SE, Staples AK, Steed IL, Stowe EL, Stueven NA, Swartz PT, Sweet EA, Sweetman AT, Tender C, Terry K, Thomas C, Thomas DS, Thompson AR, Vanderveen L, Varma R, Vaught HL, Vo QD, Vonberg ZT, Ware VC, Warrad YM, Wathen KE, Weinstein JL, Wyper JF, Yankauskas JR, Zhang C, Hatfull GF.	PLoS One	10.1371/journal.pone.0180517	2017
Pathogenicity	An antifungal protein from the marine bacterium streptomyces sp. Strain AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp.	Woo JH, Kitamura E, Myouga H, Kamei Y.	Appl Environ Microbiol	10.1128/aem.68.6.2666-2675.2002	2002
	Draft Genome Sequence of the Gram-Positive Neutrophilic Iron-Precipitating Kineosporia sp. Strain A_224.	Braun B, Kunzel S, Szewzyk U.	Genome Announc	10.1128/genomea.00763-17	2017
Phylogeny	A new neutral polymer from the cell wall of actinomycete Kineosporia aurantiaca VKM Ac-702T.	Tul'skaya EM, Senchenkova SN, Evtushenko LI, Shashkov AS, Naumova IB	Carbohydr Res	10.1016/j.carres.2005.02.002	2005
Phylogeny	Kineococcus terrestris sp. nov. and Kineococcus aureolus sp. nov., isolated from saline sediment.	Xu FJ, Li QY, Li GD, Chen X, Jiang Y, Jiang CL.	Int J Syst Evol Microbiol	10.1099/ijsem.0.002380	2017
Phylogeny	Streptomyces fractus sp. nov., a novel streptomycete isolated from the gut of a South African termite.	Rohland J, Meyers PR.	Antonie Van Leeuwenhoek	10.1007/s10482-015-0404-8	2015
Enzymology	Glycomyces phytohabitans sp. nov., a novel endophytic actinomycete isolated from the coastal halophyte in Jiangsu, East China.	Xing K, Qin S, Zhang WD, Cao CL, Ruan JS, Huang Y, Jiang JH.	J Antibiot (Tokyo)	10.1038/ja.2014.40	2014
Phylogeny	Saccharothrix yanglingensis sp. nov., an antagonistic endophytic actinomycete isolated from cucumber plant.	Yan X, Huang LL, Tu X, Gao XN, Kang ZS.	Antonie Van Leeuwenhoek	10.1007/s10482-011-9631-9	2012
Phylogeny	Kineococcus endophytica sp. nov., a novel endophytic actinomycete isolated from a coastal halophyte in Jiangsu, China.	Bian GK, Feng ZZ, Qin S, Xing K, Wang Z, Cao CL, Liu CH, Dai CC, Jiang JH.	Antonie Van Leeuwenhoek	10.1007/s10482-012-9757-4	2012
Phylogeny	Micromonospora marina sp. nov., isolated from sea sand.	Tanasupawat S, Jongrungruangchok S, Kudo T.	Int J Syst Evol Microbiol	10.1099/ijs.0.014068-0	2010
Phylogeny	Description of four new species of the genus Kineosporia: Kineosporia succinea sp. nov., Kineosporia rhizophila sp. nov., Kineosporia mikuniensis sp. nov. and Kineosporia rhamnosa sp. nov., isolated from plant samples, and amended description of the genus Kineosporia.	Kudo T, Matsushima K, Itoh T, Sasaki J, Suzuki K.	Int J Syst Bacteriol	10.1099/00207713-48-4-1245	1998