Name: Kineococcus radiotolerans SRS30216
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6317

Type strain:

Species: Kineococcus radiotolerans

Strain Designation: SRS30216, SRS 30216

Culture col. no.: DSM 14245, ATCC BAA-149, JCM 12686, CIP 107704, NBRC 101839

Strain history: CIP <- 2002, RW Phillips, Georgia Univ., Georgia, USA: strain SRS 30216 <- CJ Berry

NCBI tax ID(s): 131568 (species)

SI-ID 100703

ATCC BAA-149, DSM 14245, JCM 12686, CIP 107704, NBRC 101839

Section

Kineococcus radiotolerans SRS30216 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from Savannah River Site.

coccus-shaped
Gram-positive
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kineosporiales*
	Family	*Kineosporiaceae*
	Genus	*Kineococcus*
	Species	**Kineococcus radiotolerans**
	Full Scientific Name (LPSN)	Kineococcus radiotolerans Phillips et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #122127]	Gram stain	positive

[Ref.: #122127]	Cell shape	coccus-shaped

[Ref.: #122127]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5268]

Culture medium

PTYG-MEDIUM (DSMZ Medium 914)

[Ref.: #5268]

Culture medium growth

[Ref.: #5268]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5268]

Culture medium composition

expand / minimize

[Ref.: #33857]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #33857]

Culture medium growth

[Ref.: #33857]

Culture medium composition

expand / minimize

[Ref.: #122127]

Culture medium

CIP Medium 29

[Ref.: #122127]

Culture medium growth

[Ref.: #122127]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #122127]

Culture medium

CIP Medium 72

[Ref.: #122127]

Culture medium growth

[Ref.: #122127]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5268]		growth	30 °C
[Ref.: #33857]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #122127]		growth	22-37 °C
[Ref.: #122127]	no	growth	5 °C
[Ref.: #122127]	no	growth	15 °C
[Ref.: #122127]	no	growth	41 °C

[Ref.: #5268]	Temperature range	mesophilic
[Ref.: #33857]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #122127]	Temperature range	psychrophilic
[Ref.: #122127]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122127]

Oxygen tolerance

obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #122127]	NaCl		growth	0	%
[Ref.: #122127]	NaCl	no	growth	2	%
[Ref.: #122127]	NaCl	no	growth	4	%
[Ref.: #122127]	NaCl	no	growth	6	%
[Ref.: #122127]	NaCl	no	growth	8	%
[Ref.: #122127]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122127]	citrate ChEBI	-	carbon source
[Ref.: #122127]	esculin ChEBI	+	hydrolysis
[Ref.: #122127]	nitrate ChEBI	-	reduction
[Ref.: #122127]	nitrate ChEBI	-	respiration
[Ref.: #122127]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #122127]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #122127]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122127]	amylase	+
[Ref.: #122127]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122127]	caseinase	-	3.4.21.50
[Ref.: #122127]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #122127]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122127]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #122127]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #122127]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122127]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #122127]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122127]	tryptophan deaminase	-
[Ref.: #122127]	tween esterase	-
[Ref.: #122127]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #122127]	2393	not determinedn.d.	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	-	+	+	-	-	-	-	+	+	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122127]	10583	-	-	+	+	-	+	-	-	-	-	+	+	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5268]	Sample type/isolated from	Savannah River Site
[Ref.: #5268]	Geographic location (country and/or sea, region)	South Caroline, Aiken
[Ref.: #5268]	Country	USA
[Ref.: #5268]	Country ISO 3 Code	USA
[Ref.: #5268]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Radioactive environment at the Savannah River Site
[Ref.: #67770]	Geographic location (country and/or sea, region)	Aiken, SC
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #122127]	Sample type/isolated from	Swab of radioactive site
[Ref.: #122127]	Geographic location (country and/or sea, region)	South Carolina
[Ref.: #122127]	Country	United States of America
[Ref.: #122127]	Country ISO 3 Code	USA
[Ref.: #122127]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#River (Creek)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF247813 (>99% sequence identity) for Kineococcus from Microbeatlas

Aquatic Samples	4169
Soil Samples	9984
Animal Samples	11137
Plant Samples	6930
Total Samples	32220

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5268]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122127]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5268]	Kineococcus-like str. SRS30216 16S ribosomal RNA gene, partial sequence	AF247813	1375	ENA	266940 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Kineococcus radiotolerans SRS30216 = ATCC BAA-149	GCA_000017305	complete	GenBank	266940 tax ID
[Ref.: #66792]	Kineococcus radiotolerans SRS30216	266940.11	complete	PATRIC	266940 tax ID	*
[Ref.: #66792]	Kineococcus radiotolerans SRS30216	640753031	complete	JGI IMG/M	266940 tax ID	*

[Ref.: #67770]

GC-content

74.2 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Kineococcus radiotolerans SRS30216 PATRIC: 266940.11
[Ref.: #69480]	motile	yes	70.706	no
[Ref.: #69480]	gram-positive	yes	92.167	no
[Ref.: #69480]	anaerobic	no	99.261	no
[Ref.: #69480]	aerobic	yes	91.988	no
[Ref.: #69480]	halophile	no	90.513	no
[Ref.: #69480]	spore-forming	no	79.942	no
[Ref.: #69480]	glucose-util	yes	86.979	no
[Ref.: #69480]	flagellated	no	91.145	no
[Ref.: #69480]	thermophile	no	99.383	yes
[Ref.: #69480]	glucose-ferment	no	74.257	no

Availability in culture collections External links

[Ref.: #5268]

Culture collection no.

DSM 14245, ATCC BAA-149, JCM 12686, CIP 107704, NBRC 101839

[Ref.: #75791]

SI-ID 100703

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	A Novel Actinobacterial Cutinase Containing a Noncatalytic Polymer-Binding Domain.	Abokitse K, Grosse S, Leisch H, Corbeil CR, Perrin-Sarazin F, Lau PCK	Appl Environ Microbiol	10.1128/AEM.01522-21	2021	*
Phylogeny	Kineococcus endophytica sp. nov., a novel endophytic actinomycete isolated from a coastal halophyte in Jiangsu, China.	Bian GK, Feng ZZ, Qin S, Xing K, Wang Z, Cao CL, Liu CH, Dai CC, Jiang JH	Antonie Van Leeuwenhoek	10.1007/s10482-012-9757-4	2012	*
Genetics	Detection and isolation of selected genes of interest from metagenomic libraries by a DNA microarray approach.	Pathak GP, Gartner W	Methods Mol Biol	10.1007/978-1-60761-823-2_21	2010	*
Phylogeny	Kineococcus rhizosphaerae sp. nov., isolated from rhizosphere soil.	Lee SD	Int J Syst Evol Microbiol	10.1099/ijs.0.008599-0	2009	*
Genetics	Novel blue light-sensitive proteins from a metagenomic approach.	Pathak GP, Ehrenreich A, Losi A, Streit WR, Gartner W	Environ Microbiol	10.1111/j.1462-2920.2009.01967.x	2009	*
Genetics	Survival in nuclear waste, extreme resistance, and potential applications gleaned from the genome sequence of Kineococcus radiotolerans SRS30216.	Bagwell CE, Bhat S, Hawkins GM, Smith BW, Biswas T, Hoover TR, Saunders E, Han CS, Tsodikov OV, Shimkets LJ	PLoS One	10.1371/journal.pone.0003878	2008	*
Phylogeny	Kineococcus gynurae sp. nov., isolated from a Thai medicinal plant.	Duangmal K, Thamchaipenet A, Ara I, Matsumoto A, Takahashi Y	Int J Syst Evol Microbiol	10.1099/ijs.0.65671-0	2008	*
Phylogeny	Kineococcus radiotolerans sp. nov., a radiation-resistant, gram-positive bacterium.	Phillips RW, Wiegel J, Berry CJ, Fliermans C, Peacock AD, White DC, Shimkets LJ	Int J Syst Evol Microbiol	10.1099/00207713-52-3-933	2002	*

References

#5268

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14245

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33857 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#75791

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100703.1 )

#122127 Collection of Institut Pasteur ; Curators of the CIP; CIP 107704
* These data were automatically processed and therefore are not curated

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