Name: Knoellia flava TL1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6272

Type strain:

Species: Knoellia flava

Strain Designation: TL1

Culture col. no.: DSM 25785, CGMCC 1.10749, KCTC 19810, JCM 18318

Strain history: <- X Yu, Huzhong Agriculture Univ., China

NCBI tax ID(s): 913969 (species)

SI-ID 406369

DSM 25785

Section

Knoellia flava TL1 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from pig manure.

Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Intrasporangiaceae*
	Genus	*Knoellia*
	Species	**Knoellia flava**
	Full Scientific Name (LPSN)	Knoellia flava Yu et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30215]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #30215]	Cell length	1.0-1.9 µm

[Ref.: #30215]	Cell width	0.6 µm

[Ref.: #30215]	Cell shape	rod-shaped

[Ref.: #30215]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.378

[Ref.: #69324]	Colony color	Clay brown (8003)
[Ref.: #69324]	Cultivation medium used	ISP 7

[Ref.: #69324]	Colony color	Beige (1001)
[Ref.: #69324]	Cultivation medium used	ISP 5

[Ref.: #69324]	Colony color	Light ivory (1015)
[Ref.: #69324]	Cultivation medium used	suter without tyrosine

[Ref.: #69324]	Colony color	Ochre brown (8001)
[Ref.: #69324]	Cultivation medium used	suter with tyrosine

[Ref.: #69324]	Colony color	Sand yellow (1002)
[Ref.: #69324]	Cultivation medium used	ISP 2

[Ref.: #69324]	Colony color	Sand yellow (1002)
[Ref.: #69324]	Cultivation medium used	ISP 6

[Ref.: #69324]	Colony color	Signal yellow (1003)
[Ref.: #69324]	Cultivation medium used	ISP 3

[Ref.: #30215]	Pigment production	yes
[Ref.: #69324]	Pigment production	no
[Ref.: #69324]	Pigment name	Melanin
[Ref.: #69324]	Pigment production	yes
[Ref.: #69324]	Pigmentation color	Clay brown (8003), ochre brown (8001)
[Ref.: #69324]	Pigment name	soluble pigment

[Ref.: #69324]	Multicellular complex forming ability	no
[Ref.: #69324]	Multicellular complex name	Aerial mycelium
[Ref.: #69324]	Medium Name (multicellularity)	ISP 2

[Ref.: #69324]	Multicellular complex forming ability	no
[Ref.: #69324]	Multicellular complex name	Aerial mycelium
[Ref.: #69324]	Medium Name (multicellularity)	ISP 3

[Ref.: #69324]	Multicellular complex forming ability	no
[Ref.: #69324]	Multicellular complex name	Aerial mycelium
[Ref.: #69324]	Medium Name (multicellularity)	ISP 5

[Ref.: #69324]	Multicellular complex forming ability	no
[Ref.: #69324]	Multicellular complex name	Aerial mycelium
[Ref.: #69324]	Medium Name (multicellularity)	ISP 6

[Ref.: #69324]	Multicellular complex forming ability	no
[Ref.: #69324]	Multicellular complex name	Aerial mycelium
[Ref.: #69324]	Medium Name (multicellularity)	ISP 7

[Ref.: #69324]	Multicellular complex forming ability	no
[Ref.: #69324]	Multicellular complex name	Aerial mycelium
[Ref.: #69324]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69324]	Multicellular complex forming ability	no
[Ref.: #69324]	Multicellular complex name	Aerial mycelium
[Ref.: #69324]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69324]

Caption:

Plates (92, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69324]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69324]

Caption:

Plates (92, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69324]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5447]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #5447]

Culture medium growth

[Ref.: #5447]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5447]

Culture medium composition

expand / minimize

[Ref.: #5447]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #5447]

Culture medium growth

[Ref.: #5447]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5447]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #5447]	growth	28 °C
[Ref.: #30215]	growth	04-37 °C
[Ref.: #30215]	optimum	28 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #5447]	Temperature range	mesophilic
[Ref.: #30215]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30215]			optimum	7
[Ref.: #30215]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30215]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #30215]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.978

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30215]		NaCl		growth	<5	%
[Ref.: #69324]		NaCl		growth	0-2.5	%

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30215]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #30215]	alanine ChEBI	+	carbon source
[Ref.: #69324]	arabinose ChEBI	-	growth
[Ref.: #69324]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30215]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69324]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #30215]	glucose ChEBI	+	carbon source
[Ref.: #69324]	glucose ChEBI	+	growth
[Ref.: #30215]	glycogen ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #30215]	histidine ChEBI	+	carbon source
[Ref.: #69324]	inositol ChEBI	+/-	growth
[Ref.: #30215]	lactate ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30215]	malate ChEBI	+	carbon source
[Ref.: #30215]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30215]	mannitol ChEBI	+	carbon source
[Ref.: #30215]	mannose ChEBI	+	carbon source
[Ref.: #69324]	mannose ChEBI	+	growth
[Ref.: #30215]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #30215]	proline ChEBI	+	carbon source
[Ref.: #30215]	propionate ChEBI	+	carbon source
[Ref.: #69324]	raffinose ChEBI	+/-	growth
[Ref.: #69324]	rhamnose ChEBI	-	growth
[Ref.: #30215]	salicin ChEBI	+	carbon source
[Ref.: #30215]	serine ChEBI	+	carbon source
[Ref.: #30215]	sorbitol ChEBI	+	carbon source
[Ref.: #30215]	sucrose ChEBI	+	carbon source
[Ref.: #69324]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30215]	tween 20 ChEBI	+	carbon source
[Ref.: #30215]	tween 80 ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #30215]	valerate ChEBI	+	carbon source
[Ref.: #69324]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #30215]		hydrogen sulfide ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #30215]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #30215]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne
[Ref.: #30215]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #30215]	gelatinase	+
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69324]	3817	-	-	-	-	-	-	+	-	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69324]	6001	not determinedn.d.	-	+/-	+/-	-	+/-	-	-	-	-	-	-	-	+/-	+/-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5447]	Sample type/isolated from	pig manure
[Ref.: #5447]	Geographic location (country and/or sea, region)	Wuhan
[Ref.: #5447]	Country	China
[Ref.: #5447]	Country ISO 3 Code	CHN
[Ref.: #5447]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Pig manure
[Ref.: #67770]	Geographic location (country and/or sea, region)	Wuhan
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From pig manure
[Ref.: #67771]	Geographic location (country and/or sea, region)	Wuhan city, Hubei Province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ401007 (>99% sequence identity) for Knoellia from Microbeatlas

Aquatic Samples	168
Soil Samples	279
Animal Samples	1084
Plant Samples	34
Total Samples	1565

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5447]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5447]	Knoellia flava TL1 16S ribosomal RNA gene, partial sequence	HQ401007	1484	ENA	1385518 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Knoellia flava TL1	GCA_000768675	contig	GenBank	1385518 tax ID
[Ref.: #66792]	Knoellia flava CGMCC 1.10749	GCA_014636255	scaffold	GenBank	913969 tax ID	*
[Ref.: #66792]	Knoellia flava TL1	1385518.3	wgs	PATRIC	1385518 tax ID	*
[Ref.: #66792]	Knoellia flava strain CGMCC 1.10749	913969.3	wgs	PATRIC	913969 tax ID	*
[Ref.: #66792]	Knoellia flava TL1	2630968446	draft	JGI IMG/M	1385518 tax ID	*

[Ref.: #5447]	GC-content	70.9 mol%
[Ref.: #67770]	GC-content	70.9 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Knoellia flava CGMCC 1.10749 NCBI: GCA_014636255.1
[Ref.: #69481]	spore-forming	no	86	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Knoellia flava CGMCC 1.10749 NCBI: GCA_014636255.1
[Ref.: #69480]	flagellated	no	98.228	yes
[Ref.: #69480]	gram-positive	yes	91.698	no
[Ref.: #69480]	anaerobic	no	98.621	yes
[Ref.: #69480]	aerobic	yes	92.842	yes
[Ref.: #69480]	halophile	no	94.423	no
[Ref.: #69480]	spore-forming	no	89.978	yes
[Ref.: #69480]	glucose-util	yes	86.243	yes
[Ref.: #69480]	thermophile	no	95.107	yes
[Ref.: #69480]	motile	no	93.771	yes
[Ref.: #69480]	glucose-ferment	no	90.941	yes

Availability in culture collections External links

[Ref.: #5447]

Culture collection no.

DSM 25785, CGMCC 1.10749, KCTC 19810, JCM 18318

[Ref.: #75749]

SI-ID 406369

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Knoellia flava sp. nov., isolated from pig manure.	Yu X, Du Y, Wang G	Int J Syst Evol Microbiol	10.1099/ijs.0.030932-0	2011	*

References

#5447

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25785

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30215

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26559 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69324

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75749

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406369.1 )

#26559

IJSEM 384 2012 ( DOI 10.1099/ijs.0.030932-0 , PubMed 21441369 )

* These data were automatically processed and therefore are not curated

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Knoellia flava CGMCC 1.10749

Knoellia flava CGMCC 1.10749

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