Name: Knoellia aerolata 5317S-21
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6271

Type strain:

Species: Knoellia aerolata

Strain Designation: 5317S-21

Culture col. no.: DSM 18566, KACC 20583, JCM 16377, NBRC 106306

Strain history: KACC 20583 <-- S.-W. Kwon 5317S-21.

NCBI tax ID(s): 1385519 (strain), 442954 (species)

SI-ID 398903

DSM 18566

Section

Knoellia aerolata 5317S-21 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from air sample.

Gram-positive
ovoid-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Intrasporangiaceae*
	Genus	*Knoellia*
	Species	**Knoellia aerolata**
	Full Scientific Name (LPSN)	Knoellia aerolata Weon et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #32308]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #32308]	Cell length	1.5 µm

[Ref.: #32308]	Cell width	1 µm

[Ref.: #32308]	Cell shape	ovoid-shaped

[Ref.: #32308]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.244

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7592]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #7592]

Culture medium growth

[Ref.: #7592]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7592]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7592]	growth	28 °C
[Ref.: #18321]	optimum	28 °C
[Ref.: #32308]	growth	05-35 °C
[Ref.: #32308]	optimum	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #7592]	Temperature range	mesophilic
[Ref.: #18321]	Temperature range	mesophilic
[Ref.: #32308]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32308]			optimum	6.5
[Ref.: #32308]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32308]

Oxygen tolerance

aerobe

[Ref.: #32308]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.989

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32308]		NaCl		growth	0-2	%
[Ref.: #32308]		NaCl		optimum	0.5	%

[Ref.: #7592]	Murein short key	A31
[Ref.: #7592]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32308]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #32308]	acetate ChEBI	+	carbon source
[Ref.: #32308]	alanine ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #32308]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #32308]	gluconate ChEBI	+	carbon source
[Ref.: #32308]	glucose ChEBI	+	carbon source
[Ref.: #32308]	glycogen ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #32308]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #32308]	mannitol ChEBI	+	carbon source
[Ref.: #32308]	mannose ChEBI	+	carbon source
[Ref.: #32308]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #32308]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #32308]	proline ChEBI	+	carbon source
[Ref.: #32308]	propionate ChEBI	+	carbon source
[Ref.: #32308]	salicin ChEBI	+	carbon source
[Ref.: #32308]	sorbitol ChEBI	+	carbon source
[Ref.: #32308]	sucrose ChEBI	+	carbon source
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #32308]	tween 80 ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #32308]	valerate ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #32308]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym, API Coryne
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #32308]	gelatinase	+
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18321]	16	-	-	-	+	+	-	+	-	+	-	+	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18321]	31	not determinedn.d.	+	+	+	-	+	+	+	-	-	+	-	-	+	+	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7592]	Sample type/isolated from	air sample
[Ref.: #7592]	Geographic location (country and/or sea, region)	Suwon
[Ref.: #7592]	Country	Republic of Korea
[Ref.: #7592]	Country ISO 3 Code	KOR
[Ref.: #7592]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Air sample
[Ref.: #67770]	Geographic location (country and/or sea, region)	Suwon City
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Air

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF553529 (>99% sequence identity) for Knoellia from Microbeatlas

Aquatic Samples	244
Soil Samples	348
Animal Samples	892
Plant Samples	7
Total Samples	1491

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18321]	Biosafety level	1	German classification
[Ref.: #7592]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7592]	Knoellia aerolata strain 5317S-21 16S ribosomal RNA gene, partial sequence	EF553529	1401	ENA	442954 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Knoellia aerolata DSM 18566	GCA_000768695	contig	GenBank	1385519 tax ID
[Ref.: #66792]	Knoellia aerolata DSM 18566	1385519.3	wgs	PATRIC	1385519 tax ID	*
[Ref.: #66792]	Knoellia aerolata DSM 18566	2639762785	draft	JGI IMG/M	1385519 tax ID	*

[Ref.: #7592]	GC-content	73 mol%
[Ref.: #67770]	GC-content	71.3 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	73 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Knoellia aerolata DSM 18566 PATRIC: 1385519.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Knoellia aerolata DSM 18566 PATRIC: 1385519.3
[Ref.: #69480]	motile	no	93.88	yes
[Ref.: #69480]	gram-positive	yes	88.307	yes
[Ref.: #69480]	anaerobic	no	98.946	yes
[Ref.: #69480]	halophile	no	95.427	yes
[Ref.: #69480]	spore-forming	no	93.784	no
[Ref.: #69480]	glucose-util	yes	86.438	yes
[Ref.: #69480]	thermophile	no	96.911	yes
[Ref.: #69480]	aerobic	yes	92.628	yes
[Ref.: #69480]	flagellated	no	98.206	yes
[Ref.: #69480]	glucose-ferment	no	89.008	yes

Availability in culture collections External links

[Ref.: #7592]

Culture collection no.

DSM 18566, KACC 20583, JCM 16377, NBRC 106306

[Ref.: #75748]

SI-ID 398903

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Knoellia locipacati sp. nov., from soil of the Demilitarized Zone in South Korea.	Shin NR, Roh SW, Kim MS, Jung MJ, Whon TW, Bae JW	Int J Syst Evol Microbiol	10.1099/ijs.0.031880-0	2011	*
Phylogeny	Knoellia aerolata sp. nov., isolated from an air sample in Korea.	Weon HY, Kim BY, Schumann P, Kroppenstedt RM, Noh HJ, Park CW, Kwon SW	Int J Syst Evol Microbiol	10.1099/ijs.0.65293-0	2007	*

References

#7592

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18566

#18321

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32308

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28546 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75748

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398903.1 )

#28546

IJSEM 2861 2007 ( DOI 10.1099/ijs.0.65293-0 , PubMed 18048739 )

* These data were automatically processed and therefore are not curated

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Knoellia aerolata DSM 18566

Knoellia aerolata DSM 18566

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