Name: Campylobacter pylori RPH 13487
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6102

Type strain:

Species: Campylobacter pylori

Strain Designation: RPH 13487

Culture col. no.: CCUG 15815, LMG 7540, CIP 103995, DSM 21031, ATCC 43504, CCUG 17874, DSM 4867, LMG 7539, NCTC 11637, JCM 12093, JCM 7653, LMG 18041, LMG 18045

Strain history: CIP 103995 <-- CCUG 17874 <-- E. Blincow and C. S. Goodwin K0045659.

NCBI tax ID(s): 102618 (strain), 210 (species)

Section

Campylobacter pylori RPH 13487 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from endoscopic biopsy specimen of human gastric mucosa.

Gram-negative
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Epsilonproteobacteria*
	Order	*Campylobacterales*
	Family	*Campylobacteraceae*
	Genus	*Campylobacter*
	Species	Campylobacter pylori
	Full Scientific Name (PNU)	Campylobacter pylori corrig. Marshall et al. 1985

	Synonym	Campylobacter pyloridis
	Synonym	**Helicobacter pylori**
	Synonym	Helicobacter nemestrinae

Information on morphological and physiological properties Morphology

[Ref.: #22920]	Gram stain	negative
[Ref.: #22920]	Cell length	2.5-5 µm
[Ref.: #22920]	Cell width	0.5-1 µm
[Ref.: #22920]	Cell shape	rod-shaped
[Ref.: #22920]	Flagellum arrangement	polar

[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #69480]	Motility	yes Confidence in %93.089

[Ref.: #15441]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22920]

Culture medium

Brain heart infusion

[Ref.: #22920]

Culture medium growth

[Ref.: #15441]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #15441]

Culture medium growth

[Ref.: #15441]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #15441]

Culture medium composition

expand / minimize

[Ref.: #41863]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #41863]

Culture medium growth

[Ref.: #41863]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15441]	growth	37 °C
[Ref.: #22920]	growth	37 °C
[Ref.: #41863]	growth	37 °C
[Ref.: #46705]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #15441]	Temperature range	mesophilic
[Ref.: #22920]	Temperature range	mesophilic
[Ref.: #41863]	Temperature range	mesophilic
[Ref.: #46705]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #46705]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.997

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68373]	acetate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	citrate ChEBI	-	assimilation	* from API CAM
[Ref.: #22920]	hippurate ChEBI	-	hydrolysis
[Ref.: #68373]	malate ChEBI	-	assimilation	* from API CAM
[Ref.: #22920]	nitrate ChEBI	-	reduction
[Ref.: #68373]	nitrate ChEBI	-	reduction	* from API CAM
[Ref.: #68373]	propionate ChEBI	-	assimilation	* from API CAM
[Ref.: #22920]	urea ChEBI	+	hydrolysis
[Ref.: #68373]	urea ChEBI	+	hydrolysis	* from API CAM

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68373]	cefazolin ChEBI	yes	224	µg	* from API CAM
[Ref.: #68373]	erythromycin ChEBI	yes	14	µg	* from API CAM
[Ref.: #68373]	nalidixic acid ChEBI	yes	84	µg	* from API CAM

	Metabolite production	Metabolite
[Ref.: #22920]		hydrogen sulfide ChEBI
[Ref.: #68373]		hydrogen sulfide ChEBI	* from API CAM

	Enzyme	Enzyme activity	EC number
[Ref.: #22920]	alkaline phosphatase	-	3.1.3.1
[Ref.: #68373]	alkaline phosphatase	+	3.1.3.1	* from API CAM
[Ref.: #22920]	catalase	+	1.11.1.6
[Ref.: #15441]	catalase	+	1.11.1.6
[Ref.: #68373]	catalase	+	1.11.1.6	* from API CAM
[Ref.: #22920]	cytochrome oxidase	+	1.9.3.1
[Ref.: #15441]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68373]	gamma-glutamyltransferase	+	2.3.2.2	* from API CAM
[Ref.: #68373]	L-arginine arylamidase	-		* from API CAM
[Ref.: #68373]	L-aspartate arylamidase	-	3.4.11.21	* from API CAM
[Ref.: #22920]	leucine arylamidase	+/-	3.4.11.1
[Ref.: #68373]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API CAM
[Ref.: #22920]	urease	+	3.5.1.5
[Ref.: #68373]	urease	+	3.5.1.5	* from API CAM
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #46705]

Fatty acid	Percentage	ECL
C14:0	50.9	14
C19:0 CYCLO ω8c	19.7	18.9
C18:1 ω7c /12t/9t	6.4	17.824
C16:0 3OH	5.5	17.52
C18:0	4.6	18
C18:0 3OH	3.9	19.551
C16:0	2	16
C18:2 ω6,9c/C18:0 ANTE	1.1	17.724
C15:0	1.1	15
unknown 14.966	1.1	14.966
C13:1 at 12-13	0.9	12.931
C17:0 3OH	0.8	18.535
C12:0	0.6	12
C13:0 ISO 2OH	0.5	13.814
C19:0	0.5	19
C13:0	0.5	13

API® CAM:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Reduction of nitrateNIT	EsteraseEST	Hippuric acidHIP	gamma-Glutamyl transferaseGGT	Reduction of Triphenyl tetrazolium chlorideTTC	Pyrrolidonyl arylamidasePYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	Alkaline phosphatasePAL	H2S productionH2S	Assimilation of D-glucoseGLU	Assimilation of succinateSUT	Growth inhibitionNAL	Growth inhibitionCFZ	Assimilation of acetateACE	Assimilation of propionic acidPROP	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Erythromycin resistance (+) sensitivity (-)ERO	CatalaseCAT
[Ref.: #15441]	3592	+	-	-	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3441	+	-	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3442	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3443	+	-	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3444	+	-	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3445	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3446	+	-	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3447	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3448	+	-	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3449	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3450	+	-	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3510	+	-	-	-	+	-	-	-	-	+	-	-	+/-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3548	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3559	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3572	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #15441]	3620	+	-	-	-	+	+/-	-	-	-	+	-	+/-	-	-	-	-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15441]	Sample type/isolated from	endoscopic biopsy specimen of human gastric mucosa
[Ref.: #15441]	Country	Australia
[Ref.: #15441]	Country ISO 3 Code	AUS
[Ref.: #15441]	Continent	Australia and Oceania

[Ref.: #46705]	Sample type/isolated from	Human gastric biopsy
[Ref.: #46705]	Sampling date	1982-05-05
[Ref.: #46705]	Geographic location (country and/or sea, region)	Perth
[Ref.: #46705]	Country	Australia
[Ref.: #46705]	Country ISO 3 Code	AUS
[Ref.: #46705]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Endoscopic biopsy specimen of human gastric mucosa
[Ref.: #67770]	Geographic location (country and/or sea, region)	Royal Perth Hospital
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Infection	#Patient	#Biopsy
#Infection	#Patient	#Specimen
#Host	#Human	-
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Z25741 (>99% sequence identity) for Helicobacter pylori subclade from Microbeatlas

Aquatic Samples	829
Soil Samples	670
Animal Samples	16307
Plant Samples	278
Total Samples	18084

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15441]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15441]	Helicobacter pylori strain ATCC 43504 16S ribosomal RNA, partial sequence	NR_044761	1503	GenBank	210 tax ID
[Ref.: #67770]	Helicobacter pylori JCM 12093 gene for 16S ribosomal RNA, partial sequence	LC507447	1426	ENA	210 tax ID
[Ref.: #20218]	Helicobacter pylori isolate ATCC 43504 16S rRNA gene	U01330	1503	ENA	210 tax ID	*
[Ref.: #20218]	Helicobacter pylori (ATCC 43504) partial 16S ribosomal RNA	M88157	1450	ENA	210 tax ID	*
[Ref.: #20218]	H.pylori gene for 16S ribosomal RNA (partial)	Z25741	1454	ENA	102618 tax ID	*
[Ref.: #20218]	Helicobacter pylori 16S ribosomal RNA gene, partial sequence	AF302106	1445	ENA	210 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 = JCM 12093 CCUG 17874	GCA_000258845	contig	GenBank	102618 tax ID
[Ref.: #67770]	Helicobacter pylori strain NCTC11637 genome assembly, chromosome: 1	LS483488		ENA	102618 tax ID
[Ref.: #67770]	Helicobacter pylori FDAARGOS_299	GCA_002206465	contig	GenBank	210 tax ID
[Ref.: #66792]	Helicobacter pylori JCM 12093	GCA_014654435	scaffold	GenBank	210 tax ID	*
[Ref.: #66792]	Helicobacter pylori JCM 12093	1300157.3	wgs	PATRIC	1300157 tax ID	*
[Ref.: #66792]	Helicobacter pylori NCTC 11637 = CCUG 17874	102618.3	wgs	PATRIC	102618 tax ID	*

[Ref.: #22920]	GC-content	37.1 mol%
[Ref.: #67770]	GC-content	37.1 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 strain NCTC11637 PATRIC: 102618.7
[Ref.: #69480]	gram-positive	no	99.999	yes
[Ref.: #69480]	anaerobic	no	98.503	yes
[Ref.: #69480]	spore-forming	no	99.997	no
[Ref.: #69480]	thermophile	no	99.56	yes
[Ref.: #69480]	motile	yes	93.089	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 strain NCTC11637 PATRIC: 102618.7
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #15441]

Culture collection no.

CCUG 15815, LMG 7540, CIP 103995, DSM 21031, ATCC 43504, CCUG 17874, DSM 4867, LMG 7539, NCTC 11637, JCM 12093, JCM 7653, LMG 18041, LMG 18045

[Ref.: #20218]

Associated Passport(s) in StrainInfo

49233, 41195, 151665, 41198, 49235

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	DNA-DNA hybridization incompatibility of Campylobacter pylori with other Campylobacter and Wolinella species.	Bukholm G, Nedenskov-Sorensen P, Bovre K	APMIS		1989	*
Phylogeny	Strain variation in Campylobacter pylori detected by numerical analysis of one-dimensional electrophoretic protein patterns.	Owen RJ, Costas M, Morgan DD, On SL, Hill LR, Pearson AD, Morgan DR	Antonie Van Leeuwenhoek	10.1007/BF00393854	1989	*
Pathogenicity	Susceptibility of Campylobacter pylori to macrolides and fluoroquinolones.	Hardy DJ, Hanson CW, Hensey DM, Beyer JM, Fernandes PB	J Antimicrob Chemother	10.1093/jac/22.5.631	1988	*
Pathogenicity	GC content of DNA of Campylobacter pylori and other species belonging or related to the genus Campylobacter.	Beji A, Megraud F, Vincent P, Gavini F, Izard D, Leclerc H	Ann Inst Pasteur Microbiol	10.1016/0769-2609(88)90152-4	1988	*
	The effect of cooling rate, freeze-drying suspending fluid and culture age on the preservation of Campylobacter pylori.	Owen RJ, On SL, Costas M	J Appl Bacteriol	10.1111/j.1365-2672.1989.tb02486.x	1989	*

References

#15441

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21031

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22920

C. STEWART GOODWIN, JOHN A. ARMSTRONG, TERRY CHILVERS, MICHELLE PETERS, M. DAVID COLLINS, LINDSAY SLY, WILLIAM McCONNELL, WILLIAM E. S. HARPER: Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov., Respectively. IJSEM 39: 397 - 405 1989 ( DOI 10.1099/00207713-39-4-397 )

#41863 ; Curators of the CIP;
#46705 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 17874

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68373

Automatically annotated from API CAM .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 strain NCTC11637

Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 strain NCTC11637

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