Name: Helicobacter pylori RPH 13491
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6101

Type strain:

Species: Helicobacter pylori

Strain Designation: RPH 13491

Culture col. no.: DSM 10242, NCTC 11638, CCUG 17875, LMG 7542, CIP 107264, LMG 7541

Strain history: CIP <- 2001, CCUG <- C.S. Goodwin, Perth, Western Australia

NCBI tax ID(s): 102619 (strain), 210 (species)

SI-ID 48787

CCUG 17875, LMG 7542, NCTC 11638, LMG 7541, DSM 10242, CIP 107264

Special Collections

Other strains from Helicobacter pylori

Section

When using BacDive for research please cite our paper

Helicobacter pylori RPH 13491 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human gastric antrum.

Gram-negative
motile
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Epsilonproteobacteria*
	Order	*Campylobacterales*
	Family	*Helicobacteraceae*
	Genus	*Helicobacter*
	Species	**Helicobacter pylori**
	Full Scientific Name (LPSN)	Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989

	Synonym	Campylobacter pylori
	Synonym	Campylobacter pyloridis
	Synonym	Helicobacter nemestrinae

Information on morphological and physiological properties Morphology

[Ref.: #122082]	Gram stain	negative

[Ref.: #122082]	Cell shape	rod-shaped

[Ref.: #122082]	Motility	yes

[Ref.: #3863]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3863]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #3863]

Culture medium growth

[Ref.: #3863]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3863]

Culture medium composition

expand / minimize

[Ref.: #36116]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #36116]

Culture medium growth

[Ref.: #36116]

Culture medium composition

expand / minimize

[Ref.: #122082]

Culture medium

CIP Medium 45

[Ref.: #122082]

Culture medium growth

[Ref.: #122082]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #122082]

Culture medium

CIP Medium 6

[Ref.: #122082]

Culture medium growth

[Ref.: #122082]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3863]		growth	37 °C
[Ref.: #36116]		growth	37 °C
[Ref.: #46706]		growth	37 °C
[Ref.: #122082]		growth	37-41 °C
[Ref.: #122082]	negative	growth	5 °C
[Ref.: #122082]	negative	growth	10 °C
[Ref.: #122082]	negative	growth	25 °C
[Ref.: #122082]	negative	growth	30 °C
[Ref.: #122082]	negative	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3863]	Oxygen tolerance	microaerophile
[Ref.: #46706]	Oxygen tolerance	microaerophile
[Ref.: #122082]	Oxygen tolerance	microaerophile

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #122082]		NaCl	no	growth	3.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68373]	acetate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	D-glucose ChEBI	-	assimilation	* from API CAM
[Ref.: #122082]	hippurate ChEBI	+	hydrolysis
[Ref.: #68373]	malate ChEBI	-	assimilation	* from API CAM
[Ref.: #122082]	nitrate ChEBI	-	reduction
[Ref.: #68373]	nitrate ChEBI	-	reduction	* from API CAM
[Ref.: #122082]	nitrite ChEBI	-	reduction
[Ref.: #68373]	propionate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	urea ChEBI	+	hydrolysis	* from API CAM

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance
[Ref.: #122082]	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no			yes
[Ref.: #68373]	cefazolin ChEBI	yes	224	µg		* from API CAM
[Ref.: #68373]	erythromycin ChEBI	yes	14	µg		* from API CAM
[Ref.: #68373]	nalidixic acid ChEBI	yes	84	µg		* from API CAM

	Metabolite production	Metabolite	Production
[Ref.: #122082]		indole ChEBI	no
[Ref.: #68373]		hydrogen sulfide ChEBI	no	* from API CAM

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API CAM
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #3863]	catalase	+	1.11.1.6
[Ref.: #122082]	catalase	+	1.11.1.6
[Ref.: #68373]	catalase	+	1.11.1.6	* from API CAM
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #3863]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122082]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #68373]	gamma-glutamyltransferase	+	2.3.2.2	* from API CAM
[Ref.: #68373]	L-aspartate arylamidase	-	3.4.11.21	* from API CAM
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122082]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122082]	urease	+	3.5.1.5
[Ref.: #68373]	urease	+	3.5.1.5	* from API CAM
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® CAM:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Reduction of nitrateNIT	EsteraseEST	Hippuric acidHIP	gamma-Glutamyl transferaseGGT	Reduction of Triphenyl tetrazolium chlorideTTC	Pyrrolidonyl arylamidasePYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	Alkaline phosphatasePAL	H2S productionH2S	Assimilation of D-glucoseGLU	Assimilation of succinateSUT	Growth inhibitionNAL	Growth inhibitionCFZ	Assimilation of acetateACE	Assimilation of propionic acidPROP	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Erythromycin resistance (+) sensitivity (-)ERO	CatalaseCAT
[Ref.: #3863]	4274	+	-	-	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3863]	4080	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3863]	4079	+	-	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3863]	4078	+	-	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3863]	4077	+	-	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	+/-	-	not determinedn.d.
[Ref.: #3863]	4076	+	-	-	-	+	+	-	+	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3863]	4075	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #46706]	1581	+	-	+	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3863]	4323	+	-	-	-	+	-	+	-	-	+	-	-	+/-	-	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122082]	10211	-	+	+	+	-	+	-	+	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3863]	Sample type/isolated from	human gastric antrum
[Ref.: #3863]	Geographic location (country and/or sea, region)	Perth
[Ref.: #3863]	Country	Australia
[Ref.: #3863]	Country ISO 3 Code	AUS
[Ref.: #3863]	Continent	Australia and Oceania
[Ref.: #3863]	Geographic location	-31.9535°/115.8570°

[Ref.: #46706]	Sample type/isolated from	Human gastric biopsy
[Ref.: #46706]	Sampling date	1982-04-12
[Ref.: #46706]	Geographic location (country and/or sea, region)	Perth,Freemantle
[Ref.: #46706]	Country	Australia
[Ref.: #46706]	Country ISO 3 Code	AUS
[Ref.: #46706]	Continent	Australia and Oceania

[Ref.: #122082]	Sample type/isolated from	Human, Gastric
[Ref.: #122082]	Country	Australia
[Ref.: #122082]	Country ISO 3 Code	AUS
[Ref.: #122082]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Z25742 (>99% sequence identity) for Helicobacter pylori subclade from Microbeatlas

Aquatic Samples	829
Soil Samples	670
Animal Samples	16307
Plant Samples	278
Total Samples	18084

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3863]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #122082]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	H.pylori gene for 16S ribosomal RNA (partial)	Z25742	1454	GenBank ENA	210 tax ID	*

Availability in culture collections External links

[Ref.: #3863]

Culture collection no.

DSM 10242, NCTC 11638, CCUG 17875, LMG 7542, CIP 107264, LMG 7541

[Ref.: #75589]

SI-ID 48787

Literature:

	Title	Authors	Journal	DOI	Year
Enzymology	Campylobacter pylori isolated from the stomach of the monkey, Macaca nemestrina.	Bronsdon MA, Schoenknecht FD	J Clin Microbiol	10.1128/jcm.26.9.1725-1728.1988	1988	*
	The effect of cooling rate, freeze-drying suspending fluid and culture age on the preservation of Campylobacter pylori.	Owen RJ, On SL, Costas M	J Appl Bacteriol	10.1111/j.1365-2672.1989.tb02486.x	1989	*

References

#3863

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10242

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36116 ; Curators of the CIP;
#46706 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 17875

#68373

Automatically annotated from API CAM .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#75589

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID48787.1 )

#122082 Collection of Institut Pasteur ; Curators of the CIP; CIP 107264
* These data were automatically processed and therefore are not curated

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