Name: Kushneria avicenniae Mw2a
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6083

Type strain:

Species: Kushneria avicenniae

Strain Designation: Mw2a

Culture col. no.: DSM 23439, CECT 7193, CIP 109711, JCM 18993, CCM 7396

Strain history: CIP <- 2007, CECT <- 2006, A. Ventosa, Sevilla Univ., Sevilla, Spain <- R. Montalvo-Rodriguez, Puerto-Rico Univ., Puerto-Rico: strain MW2a

NCBI tax ID(s): 402385 (species)

SI-ID 308612

CCM 7396, CECT 7193, CIP 109711

Special Collections

Section

Kushneria avicenniae Mw2a is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from leaves of the black mangrove Avicennia germinans.

Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Halomonadaceae*
	Genus	*Kushneria*
	Species	**Kushneria avicenniae**
	Full Scientific Name (LPSN)	Kushneria avicenniae (Soto-Ramírez et al. 2007) Sánchez-Porro et al. 2009

Synonym

Halomonas avicenniae

Information on morphological and physiological properties Morphology

[Ref.: #32017]	Gram stain	negative
[Ref.: #116663]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #32017]	Cell length	2.3 µm

[Ref.: #32017]	Cell width	1.5 µm

[Ref.: #32017]	Cell shape	rod-shaped
[Ref.: #116663]	Cell shape	oval-shaped

[Ref.: #116663]	Motility	yes

[Ref.: #32017]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17211]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #17211]

Culture medium growth

[Ref.: #17211]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17211]

Culture medium composition

expand / minimize

[Ref.: #37819]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37819]

Culture medium growth

[Ref.: #37819]

Culture medium composition

expand / minimize

[Ref.: #116663]

Culture medium

CIP Medium 72

[Ref.: #116663]

Culture medium growth

[Ref.: #116663]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #116663]

Culture medium

CIP Medium 328

[Ref.: #116663]

Culture medium growth

[Ref.: #116663]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #17211]	growth	28 °C
[Ref.: #32017]	growth	12-40 °C
[Ref.: #32017]	optimum	32.5 °C
[Ref.: #37819]	growth	30 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #32017]			optimum	7.5
[Ref.: #32017]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32017]	Oxygen tolerance	aerobe
[Ref.: #116663]	Oxygen tolerance	obligate aerobe

[Ref.: #32017]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32017]		NaCl		growth	0-25	%
[Ref.: #32017]		NaCl		optimum	5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32017]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32017]	citrate ChEBI	+	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32017]	esculin ChEBI	+	hydrolysis
[Ref.: #32017]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32017]	galactose ChEBI	+	carbon source
[Ref.: #32017]	gelatin ChEBI	+	carbon source
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32017]	glucose ChEBI	+	carbon source
[Ref.: #32017]	glycerol ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32017]	maltose ChEBI	+	carbon source
[Ref.: #32017]	mannitol ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116663]	nitrate ChEBI	-	reduction
[Ref.: #116663]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32017]	sucrose ChEBI	+	carbon source
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32017]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #116663]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #32017]	catalase	+	1.11.1.6
[Ref.: #116663]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #32017]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116663]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116663]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #116663]	11637	not determinedn.d.	-	-	-	+/-	+/-	+/-	-	-	-	+/-	+/-	+/-	+/-	-	+/-	-	-	-	-	-	-	-	-	-	+/-	-	+/-	+/-	+/-	-	+/-	-	-	-	-	-	-	-	-	+/-	+/-	-	+/-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116663]	12053	-	+	+	-	-	-	-	-	-	-	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17211]	Sample type/isolated from	leaves of the black mangrove Avicennia germinans
[Ref.: #17211]	Host species	Avicennia germinans
[Ref.: #17211]	Geographic location (country and/or sea, region)	Cape Red
[Ref.: #17211]	Country	Puerto Rico
[Ref.: #17211]	Country ISO 3 Code	PRI
[Ref.: #17211]	Continent	Middle and South America

[Ref.: #67770]	Sample type/isolated from	Leaf surface of Avicennia germinans (black mangrove)
[Ref.: #67770]	Host species	Avicennia germinans

[Ref.: #116663]	Sample type/isolated from	Plant, Leaves, Avicennia germinans
[Ref.: #116663]	Geographic location (country and/or sea, region)	Cape Red, Puerto Rico
[Ref.: #116663]	Country	United States of America
[Ref.: #116663]	Country ISO 3 Code	USA
[Ref.: #116663]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ888315 (>99% sequence identity) for Kushneria avicenniae from Microbeatlas

Aquatic Samples	14
Soil Samples	3
Animal Samples	18
Plant Samples	1
Total Samples	36

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17211]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116663]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17211]	Halomonas avicenniae strain MW2a 16S ribosomal RNA gene, partial sequence	DQ888315	1433	GenBank ENA	402385 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Kushneria avicenniae DSM 23439	GCA_900112585	contig	GenBank ENA	402385 tax ID
[Ref.: #66792]	Kushneria avicenniae strain DSM 23439	402385.4	wgs	PATRIC	402385 tax ID	*
[Ref.: #66792]	Kushneria avicenniae DSM 23439	2684623008	draft	JGI IMG/M	402385 tax ID	*

[Ref.: #32017]	GC-content	61.5 mol%
[Ref.: #67770]	GC-content	61.4 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Kushneria avicenniae strain DSM 23439 PATRIC: 402385.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Kushneria avicenniae strain DSM 23439 PATRIC: 402385.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	100	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.954	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	88.366	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	83.95	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	99.286	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	76.955	no

Availability in culture collections External links

[Ref.: #17211]

Culture collection no.

DSM 23439, CECT 7193, CIP 109711, JCM 18993, CCM 7396

[Ref.: #75571]

SI-ID 308612

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halomonas avicenniae sp. nov., isolated from the salty leaves of the black mangrove Avicennia germinans in Puerto Rico.	Soto-Ramirez N, Sanchez-Porro C, Rosas S, Gonzalez W, Quinones M, Ventosa A, Montalvo-Rodriguez R	Int J Syst Evol Microbiol	10.1099/ijs.0.64818-0	2007	*

References

#17211

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23439

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32017

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28269 (see below)

#37819 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75571

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID308612.1 )

#116663 Collection of Institut Pasteur ; Curators of the CIP; CIP 109711

#28269

IJSEM 900 2007 ( DOI 10.1099/ijs.0.64818-0 , PubMed 17473230 )

* These data were automatically processed and therefore are not curated

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Kushneria avicenniae strain DSM 23439

Kushneria avicenniae strain DSM 23439

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