Acetohalobium arabaticum (DSM 5501, ATCC 49924, Z-7288)

Colony morphology

Multimedia

• @ref:	66793
  multimedia content:	EM_DSM_5501_1.jpg
  multimedia.multimedia content:	EM_DSM_5501_1.jpg
  caption:	electron microscopic image
  intellectual property rights:	© HZI/Manfred Rohde
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
2083	ACETOHALOBIUM MEDIUM (DSMZ Medium 494)		Medium recipe at MediaDive	Name: ACETOHALOBIUM MEDIUM (DSMZ Medium 494) Composition: MgCl2 x 6 H2O 3.95648 g/l Trimethylamine-HCl 2.37389 g/l Na2CO3 1.97824 g/l Na2S x 9 H2O 0.49456 g/l KCl 0.326409 g/l NH4Cl 0.326409 g/l KH2PO4 0.326409 g/l Yeast extract 0.049456 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

Culture temp

Oxygen tolerance

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	sulfopterin metabolism	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	aminopropanol phosphate biosynthesis	100	2 of 2
66794	L-lactaldehyde degradation	100	3 of 3
66794	acetate fermentation	100	4 of 4
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	cardiolipin biosynthesis	100	7 of 7
66794	coenzyme A metabolism	100	4 of 4
66794	palmitate biosynthesis	95.45	21 of 22
66794	vitamin B1 metabolism	92.31	12 of 13
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	glycolate and glyoxylate degradation	83.33	5 of 6
66794	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
66794	peptidoglycan biosynthesis	80	12 of 15
66794	methylglyoxal degradation	80	4 of 5
66794	hydrogen production	80	4 of 5
66794	cellulose degradation	80	4 of 5
66794	factor 420 biosynthesis	80	4 of 5
66794	valine metabolism	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	vitamin B12 metabolism	76.47	26 of 34
66794	purine metabolism	75.53	71 of 94
66794	glycogen biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	biotin biosynthesis	75	3 of 4
66794	glutamate and glutamine metabolism	75	21 of 28
66794	histidine metabolism	72.41	21 of 29
66794	NAD metabolism	72.22	13 of 18
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	heme metabolism	71.43	10 of 14
66794	photosynthesis	71.43	10 of 14
66794	citric acid cycle	71.43	10 of 14
66794	propanol degradation	71.43	5 of 7
66794	threonine metabolism	70	7 of 10
66794	starch degradation	70	7 of 10
66794	octane oxidation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	flavin biosynthesis	66.67	10 of 15
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	formaldehyde oxidation	66.67	2 of 3
66794	methionine metabolism	65.38	17 of 26
66794	glycolysis	64.71	11 of 17
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	pyrimidine metabolism	62.22	28 of 45
66794	alanine metabolism	62.07	18 of 29
66794	urea cycle	61.54	8 of 13
66794	cysteine metabolism	61.11	11 of 18
66794	coenzyme M biosynthesis	60	6 of 10
66794	glycogen metabolism	60	3 of 5
66794	Entner Doudoroff pathway	60	6 of 10
66794	lipoate biosynthesis	60	3 of 5
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	oxidative phosphorylation	57.14	52 of 91
66794	degradation of sugar alcohols	56.25	9 of 16
66794	non-pathway related	55.26	21 of 38
66794	arginine metabolism	54.17	13 of 24
66794	kanosamine biosynthesis II	50	1 of 2
66794	gluconeogenesis	50	4 of 8
66794	pantothenate biosynthesis	50	3 of 6
66794	ethanol fermentation	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	glutathione metabolism	50	7 of 14
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	lactate fermentation	50	2 of 4
66794	tryptophan metabolism	47.37	18 of 38
66794	leucine metabolism	46.15	6 of 13
66794	sulfate reduction	46.15	6 of 13
66794	proline metabolism	45.45	5 of 11
66794	nitrate assimilation	44.44	4 of 9
66794	CO2 fixation in Crenarchaeota	44.44	4 of 9
66794	tyrosine metabolism	42.86	6 of 14
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	propionate fermentation	40	4 of 10
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	lysine metabolism	38.1	16 of 42
66794	ketogluconate metabolism	37.5	3 of 8
66794	phenol degradation	35	7 of 20
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	methane metabolism	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	myo-inositol biosynthesis	30	3 of 10
66794	lipid metabolism	29.03	9 of 31
66794	degradation of hexoses	27.78	5 of 18
66794	vitamin B6 metabolism	27.27	3 of 11
66794	pentose phosphate pathway	27.27	3 of 11
66794	d-xylose degradation	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	cyclohexanol degradation	25	1 of 4
66794	degradation of pentoses	25	7 of 28
66794	toluene degradation	25	1 of 4
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	phenylpropanoid biosynthesis	23.08	3 of 13

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence X89077 (>99% sequence identity) for Acetohalobium arabaticum subclade from Microbeatlas

Aquatic Samples	183
Soil Samples	121
Animal Samples	2676
Plant Samples	41
Total Samples	3021

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM14469v1 assembly for Acetohalobium arabaticum DSM 5501	complete	GCA_000144695	574087.3	648028002	574087	96.14

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Acetohalobium arabaticum 16S ribosomal RNA gene, complete sequence	L37422	1475		574087
2083	H.arabaticum gene for 16S ribosomal RNA	X89077	1472		574087

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	The conserved noncoding RNA ModT coordinates growth and virulence in Clostridioides difficile.	Lence T, Sulzer J, Andress K, Gribling-Burrer AS, Lamm-Schmidt V, Barquist L, Smyth RP, Faber F.	PLoS Biol	10.1371/journal.pbio.3002948	2024
	Unraveling Anaerobic Metabolisms in a Hypersaline Sediment.	Solchaga JI, Busalmen JP, Nercessian D.	Front Microbiol	10.3389/fmicb.2022.811432	2022
	Energy conservation under extreme energy limitation: the role of cytochromes and quinones in acetogenic bacteria.	Rosenbaum FP, Muller V.	Extremophiles	10.1007/s00792-021-01241-0	2021
	Horizontal gene transfer drives the evolution of Rh50 permeases in prokaryotes.	Matassi G.	BMC Evol Biol	10.1186/s12862-016-0850-6	2017
Metabolism	The Sporomusa type Nfn is a novel type of electron-bifurcating transhydrogenase that links the redox pools in acetogenic bacteria.	Kremp F, Roth J, Muller V.	Sci Rep	10.1038/s41598-020-71038-2	2020
Metabolism	d-Ribose Catabolism in Archaea: Discovery of a Novel Oxidative Pathway in Haloarcula Species.	Johnsen U, Sutter JM, Reinhardt A, Pickl A, Wang R, Xiang H, Schonheit P.	J Bacteriol	10.1128/jb.00608-19	2020
Metabolism	Distribution of glucan-branching enzymes among prokaryotes.	Suzuki E, Suzuki R.	Cell Mol Life Sci	10.1007/s00018-016-2243-9	2016
Metabolism	Carbon source-dependent expansion of the genetic code in bacteria.	Prat L, Heinemann IU, Aerni HR, Rinehart J, O'Donoghue P, Soll D.	Proc Natl Acad Sci U S A	10.1073/pnas.1218613110	2012
	Veillonella, Firmicutes: Microbes disguised as Gram negatives.	Vesth T, Ozen A, Andersen SC, Kaas RS, Lukjancenko O, Bohlin J, Nookaew I, Wassenaar TM, Ussery DW.	Stand Genomic Sci	10.4056/sigs.2981345	2013
	Identification of protein secretion systems in bacterial genomes.	Abby SS, Cury J, Guglielmini J, Neron B, Touchon M, Rocha EP.	Sci Rep	10.1038/srep23080	2016
Enzymology	Energy-converting hydrogenases: the link between H2 metabolism and energy conservation.	Schoelmerich MC, Muller V.	Cell Mol Life Sci	10.1007/s00018-019-03329-5	2020
Genetics	Analysis of the Core Genome and Pan-Genome of Autotrophic Acetogenic Bacteria.	Shin J, Song Y, Jeong Y, Cho BK.	Front Microbiol	10.3389/fmicb.2016.01531	2016
Enzymology	Carbonic anhydrase in Acetobacterium woodii and other acetogenic bacteria.	Braus-Stromeyer SA, Schnappauf G, Braus GH, Gossner AS, Drake HL.	J Bacteriol	10.1128/jb.179.22.7197-7200.1997	1997
Metabolism	Using gas mixtures of CO, CO2 and H2 as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale.	Takors R, Kopf M, Mampel J, Bluemke W, Blombach B, Eikmanns B, Bengelsdorf FR, Weuster-Botz D, Durre P.	Microb Biotechnol	10.1111/1751-7915.13270	2018
Enzymology	The UlaG protein family defines novel structural and functional motifs grafted on an ancient RNase fold.	Fernandez FJ, Garces F, Lopez-Estepa M, Aguilar J, Baldoma L, Coll M, Badia J, Vega MC.	BMC Evol Biol	10.1186/1471-2148-11-273	2011
	Sequence and structural characterization of great salt lake bacteriophage CW02, a member of the T7-like supergroup.	Shen PS, Domek MJ, Sanz-Garcia E, Makaju A, Taylor RM, Hoggan R, Culumber MD, Oberg CJ, Breakwell DP, Prince JT, Belnap DM.	J Virol	10.1128/jvi.00407-12	2012
Genetics	Direct comparisons of Illumina vs. Roche 454 sequencing technologies on the same microbial community DNA sample.	Luo C, Tsementzi D, Kyrpides N, Read T, Konstantinidis KT.	PLoS One	10.1371/journal.pone.0030087	2012
	Composition and Activity of Microbial Communities along the Redox Gradient of an Alkaline, Hypersaline, Lake.	Edwardson CF, Edwardson CF, Hollibaugh JT.	Front Microbiol	10.3389/fmicb.2018.00014	2018
Metabolism	Net Charges of the Ribosomal Proteins of the S10 and spc Clusters of Halophiles Are Inversely Related to the Degree of Halotolerance.	Tirumalai MR, Anane-Bediakoh D, Rajesh S, Fox GE	Microbiol Spectr	10.1128/spectrum.01782-21	2021
Genetics	Complete genome sequence of Acetohalobium arabaticum type strain (Z-7288).	Sikorski J, Lapidus A, Chertkov O, Lucas S, Copeland A, Glavina Del Rio T, Nolan M, Tice H, Cheng JF, Han C, Brambilla E, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Bruce D, Detter C, Tapia R, Goodwin L, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Rohde M, Goker M, Spring S, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP	Stand Genomic Sci	10.4056/sigs.1062906	2010