Natronococcus amylolyticus (DSM 10524, JCM 9655, Ah-36)

Name: Natronococcus amylolyticus (DSM 10524, JCM 9655, Ah-36)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5966

Type strain

Strain Designation Ah-36

Culture col. no. DSM 10524 JCM 9655 Ah-36 ATCC 51971 CGMCC 1.3840 2 more

NCBI tax ID(s) 1227497 44470

Special Collections DSMZ JCM CIP

History DSMZ <- JCM <- T. Kobayashi, Nagoya University, strain Ah-36 JCM <-- T. Kudo Ah-36. CIP <- 1997, JCM <- T. Kudo: strain Ah-36

Links

Natronococcus amylolyticus Ah-36 is an obligate aerobe, Gram-positive, coccus-shaped archaeon that was isolated from soda lake.

Gram-positive coccus-shaped obligate aerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 45332

ATCC 51971,JCM 9655,CIP 105333,DSM 10524,NCIMB 13549

@ref 20215

Last LPSN update 2026-05-29 08:26:17

Domain Archaea

Phylum Methanobacteriota

Class Halobacteria

Order Halobacteriales

Family Natrialbaceae

Genus Natronococcus

Species Natronococcus amylolyticus

Full scientific name Natronococcus amylolyticus Kanai et al. 1995

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
116358	positive	coccus-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3964	NATRONOBACTERIA MEDIUM (DSMZ Medium 371)	Medium recipe at MediaDive	Name: NATRONOBACTERIA MEDIUM (DSMZ Medium 371) Composition: NaCl 200.0 g/l Agar 20.0 g/l Casamino acids 5.0 g/l Yeast extract 5.0 g/l Na2CO3 5.0 g/l Na2-glutamate 1.0 g/l KH2PO4 1.0 g/l KCl 1.0 g/l NH4Cl 1.0 g/l MgSO4 x 7 H2O 0.24 g/l CaSO4 x 2 H2O 0.17 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
39267	MEDIUM 225 - for Natronococcus, Amylolyticus and Natronobacterium gregoryi		Distilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (1.000 g);Magnesium sulphate heptahydrate (0.240 g);Agar (20.000 g);Yeastextract (5.000 g);Potassium di-hydrogen phosphate (1.000 g);Ammonium chloride (1.000 g);Sodium
116358	CIP Medium 225	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
3964	positive	growth	37
39267	positive	growth	37
67770	positive	growth	37
116358	positive	growth	25-37
116358	negative	growth	5
116358	negative	growth	15

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
116358	obligate aerobe
125439	facultative anaerobe	92.386

Spore formation

@ref	Spore formation	Confidence
125439		95.627

Compound production

@ref	Compound
3964	alpha amylase
67770	Maltotriose-forming alpha-amylase

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
116358	4853 ChEBI	esculin	-	hydrolysis
116358	606565 ChEBI	hippurate	-	hydrolysis
116358	17632 ChEBI	nitrate	-	builds gas from
116358	17632 ChEBI	nitrate	+	reduction
116358	17632 ChEBI	nitrate	-	respiration
116358	16301 ChEBI	nitrite	-	builds gas from
116358	16301 ChEBI	nitrite	-	reduction

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
116358	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
116358	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
116358	15688 ChEBI	acetoin	-
116358	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
116358	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
116358	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
116358	gamma-glutamyltransferase	+	2.3.2.2
116358	gelatinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116358	oxidase	+
116358	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
116358	tryptophan deaminase	-
116358	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116358	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Condition	#Alkaline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3964	soda lake	Lake Magadi	Kenya	KEN	Africa
67770	Soil from shores of the Lake Magadi		Kenya	KEN	Africa
116358	Environment, Soil from shares	Magadi lake	Kenya	KEN	Africa

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3964	1	Risk group (German classification) According to TRBA 466
116358	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM33767v1 assembly for Natronococcus amylolyticus DSM 10524	contig	GCA_000337675	1227497.3	2554235477	1227497	69.4

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Natronococcus amylolyticus gene for 16S rRNA and gene for alanine tRNA, complete sequence	D43628	1930		44470
20218	Natronococcus amylolyticus gene for 16S rRNA, complete sequence, strain: JCM 9655	AB663468	1474		44470

GC content

67770

GC-content (mol%)63.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM33767v1 assembly for Natronococcus amylolyticus DSM 10524
NCBI: GCA_000337675

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	92.39	no
125439	gram_stain	BacteriaNetⓘ	negative	69.05	no
125439	motility	BacteriaNetⓘ	yes	49.84	no
125439	spore_formation	BacteriaNetⓘ	no	95.63	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Dihydroxyacetone metabolism in Haloferax volcanii.	Ouellette M, Makkay AM, Papke RT.	Front Microbiol	10.3389/fmicb.2013.00376	2013
Genetics	The haloarchaeal MCM proteins: bioinformatic analysis and targeted mutagenesis of the beta7-beta8 and beta9-beta10 hairpin loops and conserved zinc binding domain cysteines.	Kristensen TP, Maria Cherian R, Gray FC, MacNeill SA.	Front Microbiol	10.3389/fmicb.2014.00123	2014
Phylogeny	Natronococcus pandeyae sp. nov., a Novel Haloarchaeon from Sambhar Salt Lake.	Kajale S, Deshpande N, Lodha T, Shouche Y, Sharma A	Curr Microbiol	10.1007/s00284-021-02740-1	2022
Phylogeny	Natronococcus roseus sp. nov., a haloalkaliphilic archaeon from a hypersaline lake.	Corral P, Gutierrez MC, Castillo AM, Dominguez M, Lopalco P, Corcelli A, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.036558-0	2012
Phylogeny	Natronococcus amylolyticus sp. nov., a haloalkaliphilic archaeon.	Kanal H, Kobayashi T, Aono R, Kudo T	Int J Syst Bacteriol	10.1099/00207713-45-4-762	1995

References

#3964	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10524
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#39267	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#116358	Collection of Institut Pasteur ; Curators of the CIP; CIP 105333
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

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Natronococcus amylolyticus (DSM 10524, JCM 9655, Ah-36)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000337675

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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