Name: Natronococcus amylolyticus Ah-36
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 5966

Type strain:

Species: Natronococcus amylolyticus

Strain Designation: Ah-36

Culture col. no.: DSM 10524, JCM 9655, ATCC 51971, CGMCC 1.3840, CIP 105333, FERM P-11024

Strain history: CIP <- 1997, JCM <- T. Kudo: strain Ah-36

NCBI tax ID(s): 1227497 (strain), 44470 (species)

SI-ID 45332

ATCC 51971, JCM 9655, CIP 105333, DSM 10524, NCIMB 13549

Special Collections

Section

Natronococcus amylolyticus Ah-36 is an obligate aerobe, Gram-positive, coccus-shaped archaeon that was isolated from soda lake.

coccus-shaped
Gram-positive
obligate aerobe
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Halobacteria*
	Order	*Natrialbales*
	Family	*Natrialbaceae*
	Genus	*Natronococcus*
	Species	**Natronococcus amylolyticus**
	Full Scientific Name (LPSN)	Natronococcus amylolyticus Kanai et al. 1995

Information on morphological and physiological properties Morphology

[Ref.: #116358]	Gram stain	positive

[Ref.: #116358]	Cell shape	coccus-shaped

[Ref.: #116358]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3964]

Culture medium

NATRONOBACTERIA MEDIUM (DSMZ Medium 371)

[Ref.: #3964]

Culture medium growth

[Ref.: #3964]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3964]

Culture medium composition

expand / minimize

[Ref.: #39267]

Culture medium

MEDIUM 225 - for Natronococcus, Amylolyticus and Natronobacterium gregoryi

[Ref.: #39267]

Culture medium growth

[Ref.: #39267]

Culture medium composition

expand / minimize

[Ref.: #116358]

Culture medium

CIP Medium 225

[Ref.: #116358]

Culture medium growth

[Ref.: #116358]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3964]		growth	37 °C
[Ref.: #39267]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #116358]		growth	25-37 °C
[Ref.: #116358]	no	growth	5 °C
[Ref.: #116358]	no	growth	15 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116358]

Oxygen tolerance

obligate aerobe

[Ref.: #3964]	Name of produced compound	alpha amylase
[Ref.: #67770]	Name of produced compound	Maltotriose-forming alpha-amylase

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116358]	esculin ChEBI	-	hydrolysis
[Ref.: #116358]	hippurate ChEBI	-	hydrolysis
[Ref.: #116358]	nitrate ChEBI	-	builds gas from
[Ref.: #116358]	nitrate ChEBI	+	reduction
[Ref.: #116358]	nitrate ChEBI	-	respiration
[Ref.: #116358]	nitrite ChEBI	-	builds gas from
[Ref.: #116358]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #116358]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #116358]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #116358]		acetoin ChEBI	-
[Ref.: #116358]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116358]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116358]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116358]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #116358]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116358]	oxidase	+
[Ref.: #116358]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116358]	tryptophan deaminase	-
[Ref.: #116358]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116358]	8623	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3964]	Sample type/isolated from	soda lake
[Ref.: #3964]	Geographic location (country and/or sea, region)	Lake Magadi
[Ref.: #3964]	Country	Kenya
[Ref.: #3964]	Country ISO 3 Code	KEN
[Ref.: #3964]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Soil from shores of the Lake Magadi
[Ref.: #67770]	Country	Kenya
[Ref.: #67770]	Country ISO 3 Code	KEN
[Ref.: #67770]	Continent	Africa

[Ref.: #116358]	Sample type/isolated from	Environment, Soil from shares
[Ref.: #116358]	Geographic location (country and/or sea, region)	Magadi lake
[Ref.: #116358]	Country	Kenya
[Ref.: #116358]	Country ISO 3 Code	KEN
[Ref.: #116358]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Condition	#Alkaline	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3964]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116358]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Natronococcus amylolyticus gene for 16S rRNA and gene for alanine tRNA, complete sequence	D43628	1930	GenBank ENA	44470 tax ID	*
[Ref.: #20218]	Natronococcus amylolyticus gene for 16S rRNA, complete sequence, strain: JCM 9655	AB663468	1474	GenBank ENA	44470 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Natronococcus amylolyticus DSM 10524	GCA_000337675	contig	GenBank ENA	1227497 tax ID
[Ref.: #66792]	Natronococcus amylolyticus DSM 10524	2554235477	draft	JGI IMG/M	1227497 tax ID	*
[Ref.: #66792]	Natronococcus amylolyticus DSM 10524	1227497.3	wgs	PATRIC	1227497 tax ID	*

[Ref.: #67770]

GC-content

63.5 mol%

Availability in culture collections External links

[Ref.: #3964]

Culture collection no.

DSM 10524, JCM 9655, ATCC 51971, CGMCC 1.3840, CIP 105333, FERM P-11024

[Ref.: #75455]

SI-ID 45332

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Natronococcus pandeyae sp. nov., a Novel Haloarchaeon from Sambhar Salt Lake.	Kajale S, Deshpande N, Lodha T, Shouche Y, Sharma A	Curr Microbiol	10.1007/s00284-021-02740-1	2022	*
Phylogeny	Natronococcus roseus sp. nov., a haloalkaliphilic archaeon from a hypersaline lake.	Corral P, Gutierrez MC, Castillo AM, Dominguez M, Lopalco P, Corcelli A, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.036558-0	2012	*
Phylogeny	Natronococcus amylolyticus sp. nov., a haloalkaliphilic archaeon.	Kanal H, Kobayashi T, Aono R, Kudo T	Int J Syst Bacteriol	10.1099/00207713-45-4-762	1995	*

References

#3964

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10524

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39267 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#75455

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID45332.1 )

#116358 Collection of Institut Pasteur ; Curators of the CIP; CIP 105333
* These data were automatically processed and therefore are not curated

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