Stackebrandtia nassauensis (DSM 44728, CIP 108903, NRRL B-16338)

Cell morphology

Colony morphology

Pigmentation

Multimedia

• @ref:	12046
  multimedia content:	DSM_44728-1.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44728-1.jpg
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

• @ref:	12046
  multimedia content:	DSM_44728.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44728.jpg
  caption:	Medium 554 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

• @ref:	66793
  multimedia content:	EM_DSM_44728_1.jpg
  multimedia.multimedia content:	EM_DSM_44728_1.jpg
  caption:	electron microscopic image
  intellectual property rights:	© HZI/Manfred Rohde
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
12046	N-Z-AMINE-MEDIUM (DSMZ Medium 554)		Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
12046	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
38363	MEDIUM 45 - for Columbia agar with sheep blood			Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
121240	CIP Medium 56		Medium recipe at CIP
121240	CIP Medium 45		Medium recipe at CIP

Culture temp

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31416	30089 ChEBI	acetate	+	carbon source
31416	22599 ChEBI	arabinose	+	carbon source
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
31416	17057 ChEBI	cellobiose	+	carbon source
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
121240	16947 ChEBI	citrate	+	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
31416	23652 ChEBI	dextrin	+	carbon source
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
31416	4853 ChEBI	esculin	+	hydrolysis
121240	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
31416	28757 ChEBI	fructose	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
31416	5291 ChEBI	gelatin	+	carbon source
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
121240	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
31416	17716 ChEBI	lactose	+	carbon source
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
31416	25115 ChEBI	malate	+	carbon source
31416	17306 ChEBI	maltose	+	carbon source
31416	37684 ChEBI	mannose	+	carbon source
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
31416	28053 ChEBI	melibiose	+	carbon source
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
31416	17632 ChEBI	nitrate	+	reduction
121240	17632 ChEBI	nitrate	-	reduction
121240	17632 ChEBI	nitrate	-	respiration
121240	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
31416	16634 ChEBI	raffinose	+	carbon source
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
31416	26546 ChEBI	rhamnose	+	carbon source
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
31416	17814 ChEBI	salicin	+	carbon source
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
31416	30911 ChEBI	sorbitol	+	carbon source
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
31416	17992 ChEBI	sucrose	+	carbon source
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
31416	27082 ChEBI	trehalose	+	carbon source
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid
31416	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121240	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
121240	15688 ChEBI	acetoin	+
121240	17234 ChEBI	glucose		+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
121240	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
121240	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
121240	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121240	caseinase	+	3.4.21.50
31416	catalase	+	1.11.1.6
121240	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
121240	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
121240	gamma-glutamyltransferase	+	2.3.2.2
31416	gelatinase	+
121240	gelatinase	+
121240	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121240	lipase	-
68382	lipase (C 14)	-		from API zym
121240	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121240	ornithine decarboxylase	-	4.1.1.17
121240	oxidase	+
121240	phenylalanine ammonia-lyase	+	4.3.1.24
121240	protease	+
68382	trypsin	+	3.4.21.4	from API zym
121240	tryptophan deaminase	-
121240	tween esterase	+
121240	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	methylglyoxal degradation	100	5 of 5
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	myo-inositol biosynthesis	100	10 of 10
66794	enterobactin biosynthesis	100	3 of 3
66794	ppGpp biosynthesis	100	4 of 4
66794	1,4-dihydroxy-6-naphthoate biosynthesis	100	6 of 6
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	threonine metabolism	100	10 of 10
66794	palmitate biosynthesis	100	22 of 22
66794	ethanol fermentation	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	aminopropanol phosphate biosynthesis	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	formaldehyde oxidation	100	3 of 3
66794	isoleucine metabolism	100	8 of 8
66794	folate polyglutamylation	100	1 of 1
66794	biotin biosynthesis	100	4 of 4
66794	glycine betaine biosynthesis	100	5 of 5
66794	phenylmercury acetate degradation	100	2 of 2
66794	cellulose degradation	100	5 of 5
66794	valine metabolism	100	9 of 9
66794	NAD metabolism	94.44	17 of 18
66794	heme metabolism	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	Entner Doudoroff pathway	90	9 of 10
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	purine metabolism	86.17	81 of 94
66794	citric acid cycle	85.71	12 of 14
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	photosynthesis	85.71	12 of 14
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	leucine metabolism	84.62	11 of 13
66794	glycolate and glyoxylate degradation	83.33	5 of 6
66794	histidine metabolism	82.76	24 of 29
66794	alanine metabolism	82.76	24 of 29
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	vitamin K metabolism	80	4 of 5
66794	3-chlorocatechol degradation	80	4 of 5
66794	hydrogen production	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	gallate degradation	80	4 of 5
66794	glycogen metabolism	80	4 of 5
66794	arginine metabolism	79.17	19 of 24
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	allantoin degradation	77.78	7 of 9
66794	4-hydroxymandelate degradation	77.78	7 of 9
66794	glycolysis	76.47	13 of 17
66794	pyrimidine metabolism	75.56	34 of 45
66794	butanoate fermentation	75	3 of 4
66794	lactate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	tryptophan metabolism	73.68	28 of 38
66794	methionine metabolism	73.08	19 of 26
66794	proline metabolism	72.73	8 of 11
66794	d-xylose degradation	72.73	8 of 11
66794	degradation of sugar acids	72	18 of 25
66794	glutathione metabolism	71.43	10 of 14
66794	propanol degradation	71.43	5 of 7
66794	tyrosine metabolism	71.43	10 of 14
66794	non-pathway related	71.05	27 of 38
66794	lipid metabolism	70.97	22 of 31
66794	oxidative phosphorylation	69.23	63 of 91
66794	urea cycle	69.23	9 of 13
66794	vitamin B1 metabolism	69.23	9 of 13
66794	lysine metabolism	69.05	29 of 42
66794	degradation of pentoses	67.86	19 of 28
66794	nitrate assimilation	66.67	6 of 9
66794	daunorubicin biosynthesis	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	degradation of hexoses	66.67	12 of 18
66794	methane metabolism	66.67	2 of 3
66794	degradation of aromatic, nitrogen containing compounds	66.67	8 of 12
66794	acetoin degradation	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	cysteine metabolism	66.67	12 of 18
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	metabolism of disaccharids	63.64	7 of 11
66794	vitamin B6 metabolism	63.64	7 of 11
66794	ketogluconate metabolism	62.5	5 of 8
66794	degradation of sugar alcohols	62.5	10 of 16
66794	phosphatidylethanolamine bioynthesis	61.54	8 of 13
66794	lipoate biosynthesis	60	3 of 5
66794	coenzyme M biosynthesis	60	6 of 10
66794	creatinine degradation	60	3 of 5
66794	phenol degradation	60	12 of 20
66794	starch degradation	60	6 of 10
66794	D-cycloserine biosynthesis	60	3 of 5
66794	sulfate reduction	53.85	7 of 13
66794	phenylpropanoid biosynthesis	53.85	7 of 13
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	pantothenate biosynthesis	50	3 of 6
66794	vitamin E metabolism	50	2 of 4
66794	quinate degradation	50	1 of 2
66794	resorcinol degradation	50	1 of 2
66794	toluene degradation	50	2 of 4
66794	ribulose monophosphate pathway	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	CMP-KDO biosynthesis	50	2 of 4
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	cyclohexanol degradation	50	2 of 4
66794	glycine metabolism	50	5 of 10
66794	catecholamine biosynthesis	50	2 of 4
66794	polyamine pathway	47.83	11 of 23
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	lipid A biosynthesis	44.44	4 of 9
66794	androgen and estrogen metabolism	43.75	7 of 16
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	bacilysin biosynthesis	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	arachidonic acid metabolism	38.89	7 of 18
66794	ascorbate metabolism	36.36	8 of 22
66794	IAA biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	sulfoquinovose degradation	33.33	1 of 3
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	vitamin B12 metabolism	29.41	10 of 34
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	chlorophyll metabolism	27.78	5 of 18
66794	cholesterol biosynthesis	27.27	3 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	carotenoid biosynthesis	27.27	6 of 22
66794	carnitine metabolism	25	2 of 8
66794	alginate biosynthesis	25	1 of 4

API zym

API 50CHac

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence AY650268 (>99% sequence identity) for Stackebrandtia nassauensis subclade from Microbeatlas

Aquatic Samples	19
Soil Samples	435
Animal Samples	96
Plant Samples	187
Total Samples	737

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2454v1 assembly for Stackebrandtia nassauensis DSM 44728	complete	GCA_000024545	446470.6	646564571	446470	98.09

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
12046	Stackebrandtia nassauensis strain NRRL B-16338 16S ribosomal RNA gene, partial sequence	AY650268	1491		446470

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Role of fourteen XRE-DUF397 pairs from Streptomyces coelicolor as regulators of antibiotic production and differentiation. New players in a complex regulatory network.	Riascos C, Martinez-Carrasco A, Diaz M, Santamaria RI.	Front Microbiol	10.3389/fmicb.2023.1217350	2023
Metabolism	Characterization of Stackebrandtia nassauensis GH 20 Beta-Hexosaminidase, a Versatile Biocatalyst for Chitobiose Degradation.	Wang M, Zheng F, Wang T, Lyu YM, Alteen MG, Cai ZP, Cui ZL, Liu L, Voglmeir J.	Int J Mol Sci	10.3390/ijms20051243	2019
	Occurrence of vanHAX and Related Genes beyond the Actinobacteria Phylum.	Yushchuk O, Binda E, Fedorenko V, Marinelli F.	Genes (Basel)	10.3390/genes13111960	2022
	The Supersized Class III Lanthipeptide Stackepeptin Displays Motif Multiplication in the Core Peptide.	Jungmann NA, van Herwerden EF, Hugelland M, Sussmuth RD.	ACS Chem Biol	10.1021/acschembio.5b00651	2016
	Influence of Intratumor Microbiome on Clinical Outcome and Immune Processes in Prostate Cancer.	Ma J, Gnanasekar A, Lee A, Li WT, Haas M, Wang-Rodriguez J, Chang EY, Rajasekaran M, Ongkeko WM.	Cancers (Basel)	10.3390/cancers12092524	2020
Metabolism	Microbiome in cancer: An exploration of carcinogenesis, immune responses and immunotherapy.	Zhou P, Hu Y, Wang X, Shen L, Liao X, Zhu Y, Yu J, Zhao F, Zhou Y, Shen H, Li J.	Front Immunol	10.3389/fimmu.2022.877939	2022
	Streptomyces venezuelae ISP5230 Maintains Excretion of Jadomycin upon Disruption of the MFS Transporter JadL Located within the Natural Product Biosynthetic Gene Cluster.	Forget SM, McVey J, Vining LC, Jakeman DL.	Front Microbiol	10.3389/fmicb.2017.00432	2017
Metabolism	Genes associated with 2-methylisoborneol biosynthesis in cyanobacteria: isolation, characterization, and expression in response to light.	Wang Z, Xu Y, Shao J, Wang J, Li R.	PLoS One	10.1371/journal.pone.0018665	2011
	Candidatus Frankia Datiscae Dg1, the Actinobacterial Microsymbiont of Datisca glomerata, Expresses the Canonical nod Genes nodABC in Symbiosis with Its Host Plant.	Persson T, Battenberg K, Demina IV, Vigil-Stenman T, Vanden Heuvel B, Pujic P, Facciotti MT, Wilbanks EG, O'Brien A, Fournier P, Cruz Hernandez MA, Mendoza Herrera A, Medigue C, Normand P, Pawlowski K, Berry AM.	PLoS One	10.1371/journal.pone.0127630	2015
Metabolism	CO synthesized from the central one-carbon pool as source for the iron carbonyl in O2-tolerant [NiFe]-hydrogenase.	Burstel I, Siebert E, Frielingsdorf S, Zebger I, Friedrich B, Lenz O.	Proc Natl Acad Sci U S A	10.1073/pnas.1614656113	2016
Enzymology	Molecular cloning, heterologous expression, and enzymatic characterization of lysoplasmalogen-specific phospholipase D from Thermocrispum sp.	Matsumoto Y, Kashiwabara N, Oyama T, Murayama K, Matsumoto H, Sakasegawa SI, Sugimori D.	FEBS Open Bio	10.1002/2211-5463.12131	2016
Genetics	Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis.	Hu D, Sun C, Jin T, Fan G, Mok KM, Li K, Lee SM.	Front Microbiol	10.3389/fmicb.2020.01540	2020
	Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663.	Saleh O, Flinspach K, Westrich L, Kulik A, Gust B, Fiedler HP, Heide L.	Beilstein J Org Chem	10.3762/bjoc.8.57	2012
	Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development.	Sandoval-Calderon M, Nguyen DD, Kapono CA, Herron P, Dorrestein PC, Sohlenkamp C.	Front Microbiol	10.3389/fmicb.2015.01465	2015
Metabolism	The futalosine pathway played an important role in menaquinone biosynthesis during early prokaryote evolution.	Zhi XY, Yao JC, Tang SK, Huang Y, Li HW, Li WJ.	Genome Biol Evol	10.1093/gbe/evu007	2014
Metabolism	Comparative genomics study of polyhydroxyalkanoates (PHA) and ectoine relevant genes from Halomonas sp. TD01 revealed extensive horizontal gene transfer events and co-evolutionary relationships.	Cai L, Tan D, Aibaidula G, Dong XR, Chen JC, Tian WD, Chen GQ.	Microb Cell Fact	10.1186/1475-2859-10-88	2011
Enzymology	Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community.	Allgaier M, Reddy A, Park JI, Ivanova N, D'haeseleer P, Lowry S, Sapra R, Hazen TC, Simmons BA, VanderGheynst JS, Hugenholtz P.	PLoS One	10.1371/journal.pone.0008812	2010
Transcriptome	Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space.	Helbert W, Poulet L, Drouillard S, Mathieu S, Loiodice M, Couturier M, Lombard V, Terrapon N, Turchetto J, Vincentelli R, Henrissat B.	Proc Natl Acad Sci U S A	10.1073/pnas.1815791116	2019
Transcriptome	In silico analysis highlights the frequency and diversity of type 1 lantibiotic gene clusters in genome sequenced bacteria.	Marsh AJ, O'Sullivan O, Ross RP, Cotter PD, Hill C.	BMC Genomics	10.1186/1471-2164-11-679	2010
Genetics	Sugar Lego: gene composition of bacterial carbohydrate metabolism genomic loci.	Kaznadzey A, Shelyakin P, Gelfand MS.	Biol Direct	10.1186/s13062-017-0200-7	2017
Transcriptome	MetaID: a novel method for identification and quantification of metagenomic samples.	Srinivasan SM, Guda C.	BMC Genomics	10.1186/1471-2164-14-s8-s4	2013
Phylogeny	Related giant viruses in distant locations and different habitats: Acanthamoeba polyphaga moumouvirus represents a third lineage of the Mimiviridae that is close to the megavirus lineage.	Yoosuf N, Yutin N, Colson P, Shabalina SA, Pagnier I, Robert C, Azza S, Klose T, Wong J, Rossmann MG, La Scola B, Raoult D, Koonin EV.	Genome Biol Evol	10.1093/gbe/evs109	2012
Metabolism	Genomics of aerobic cellulose utilization systems in actinobacteria.	Anderson I, Abt B, Lykidis A, Klenk HP, Kyrpides N, Ivanova N.	PLoS One	10.1371/journal.pone.0039331	2012
Phylogeny	A novel taxonomic marker that discriminates between morphologically complex actinomycetes.	Girard G, Traag BA, Sangal V, Mascini N, Hoskisson PA, Goodfellow M, van Wezel GP.	Open Biol	10.1098/rsob.130073	2013
Genetics	BAGEL2: mining for bacteriocins in genomic data.	de Jong A, van Heel AJ, Kok J, Kuipers OP.	Nucleic Acids Res	10.1093/nar/gkq365	2010
Genetics	Comparative genomics reveals evidence of marine adaptation in Salinispora species.	Penn K, Jensen PR.	BMC Genomics	10.1186/1471-2164-13-86	2012
Phylogeny	The plasmid-mediated evolution of the mycobacterial ESX (Type VII) secretion systems.	Newton-Foot M, Warren RM, Sampson SL, van Helden PD, Gey van Pittius NC.	BMC Evol Biol	10.1186/s12862-016-0631-2	2016
Genetics	Developmental biology of Streptomyces from the perspective of 100 actinobacterial genome sequences.	Chandra G, Chater KF.	FEMS Microbiol Rev	10.1111/1574-6976.12047	2014
Metabolism	Crystal structure of PhnF, a GntR-family transcriptional regulator of phosphate transport in Mycobacterium smegmatis.	Gebhard S, Busby JN, Fritz G, Moreland NJ, Cook GM, Lott JS, Baker EN, Money VA.	J Bacteriol	10.1128/jb.01965-14	2014
Metabolism	A mycobacterial phosphoribosyltransferase promotes bacillary survival by inhibiting oxidative stress and autophagy pathways in macrophages and zebrafish.	Mohanty S, Jagannathan L, Ganguli G, Padhi A, Roy D, Alaridah N, Saha P, Nongthomba U, Godaly G, Gopal RK, Banerjee S, Sonawane A.	J Biol Chem	10.1074/jbc.m114.598482	2015
	Annotation of Protein Domains Reveals Remarkable Conservation in the Functional Make up of Proteomes Across Superkingdoms.	Nasir A, Naeem A, Khan MJ, Nicora HD, Caetano-Anolles G.	Genes (Basel)	10.3390/genes2040869	2011
Phylogeny	Bioinformatic characterization of the 4-Toluene Sulfonate Uptake Permease (TSUP) family of transmembrane proteins.	Shlykov MA, Zheng WH, Chen JS, Saier MH.	Biochim Biophys Acta	10.1016/j.bbamem.2011.12.005	2012
Phylogeny	Phylogenetic framework and molecular signatures for the main clades of the phylum Actinobacteria.	Gao B, Gupta RS.	Microbiol Mol Biol Rev	10.1128/mmbr.05011-11	2012
Metabolism	Purification and characterization of phosphonoglycans from Glycomyces sp. strain NRRL B-16210 and Stackebrandtia nassauensis NRRL B-16338.	Yu X, Price NP, Evans BS, Metcalf WW	J Bacteriol	10.1128/JB.00036-14	2014
Genetics	Complete genome sequence of Stackebrandtia nassauensis type strain (LLR-40K-21).	Munk C, Lapidus A, Copeland A, Jando M, Mayilraj S, Glavina Del Rio T, Nolan M, Chen F, Lucas S, Tice H, Cheng JF, Han C, Detter JC, Bruce D, Goodwin L, Chain P, Pitluck S, Goker M, Ovchinikova G, Pati A, Ivanova N, Mavromatis K, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP	Stand Genomic Sci	10.4056/sigs.47643	2009
Phylogeny	Stackebrandtia cavernae sp. nov., a novel actinobacterium isolated from a karst cave sample.	Zhang WQ, Li YQ, Liu L, Salam N, Fang BZ, Wei DQ, Han MX, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000859	2015
Phylogeny	Stackebrandtia albiflava sp. nov. and emended description of the genus Stackebrandtia.	Wang YX, Zhi XY, Zhang YQ, Cui XL, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.002147-0	2009
Phylogeny	Stackebrandtia nassauensis gen. nov., sp. nov. and emended description of the family Glycomycetaceae.	Labeda DP, Kroppenstedt RM	Int J Syst Evol Microbiol	10.1099/ijs.0.63496-0	2005