Name: Fusobacterium necrophorum subsp. funduliforme Fn524
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5776

Type strain:

Species: Fusobacterium necrophorum subsp. funduliforme

Strain Designation: Fn524, Fn 524, 6161

Culture col. no.: DSM 19678, ATCC 51357, CCUG 42162, CIP 104859, JCM 3724, VPI 6161

Strain history: CIP <- 1996, JCM <- T. shinjo: strain Fn 524 <- M. Kanoe <- VPI: strain 6161

NCBI tax ID(s): 1095747 (strain), 143387 (subspecies)

SI-ID 46149

ATCC 51357, JCM 3724, CIP 104859, CCUG 42162, DSM 19678

Other strains from Fusobacterium necrophorum subsp. funduliforme

F. necrophorum subsp. funduliforme CCUG 59679
F. necrophorum subsp. funduliforme CCUG 62656
F. necrophorum subsp. funduliforme JCM 3717
F. necrophorum subsp. funduliforme JCM 3719

Section

Fusobacterium necrophorum subsp. funduliforme Fn524 is an anaerobe, mesophilic human pathogen that was isolated from bovine liver abscess.

anaerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Fusobacteriota*
	Class	*Fusobacteriia*
	Order	*Fusobacteriales*
	Family	*Fusobacteriaceae*
	Genus	*Fusobacterium*
	Species	**Fusobacterium necrophorum subsp. funduliforme**
	Full Scientific Name (LPSN)	Fusobacterium necrophorum subsp. funduliforme (ex Hallé 1898) Shinjo et al. 1991

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8249]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8249]

Culture medium growth

[Ref.: #8249]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8249]

Culture medium composition

expand / minimize

[Ref.: #8249]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

[Ref.: #8249]

Culture medium growth

[Ref.: #8249]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8249]

Culture medium composition

expand / minimize

[Ref.: #41348]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #41348]

Culture medium growth

[Ref.: #41348]

Culture medium composition

expand / minimize

[Ref.: #121253]

Culture medium

CIP Medium 20

[Ref.: #121253]

Culture medium growth

[Ref.: #121253]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8249]	growth	37 °C
[Ref.: #41348]	growth	37 °C
[Ref.: #55350]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #8249]	Temperature range	mesophilic
[Ref.: #41348]	Temperature range	mesophilic
[Ref.: #55350]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8249]	Oxygen tolerance	anaerobe
[Ref.: #55350]	Oxygen tolerance	anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	+	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	yes	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	+	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	+	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #55350]

Fatty acid	Percentage	ECL
C16:0	12.3	16
C14:0	11.9	14
C14:0 3OH/C16:1 ISO I	9.7	15.485
C16:1 ω7c	9.6	15.819
C18:2 ω6,9c/C18:0 ANTE	8.3	17.724
C18:1 ω9c	5.9	17.769
C17:1 ISO I/C16:0 DMA	5.6	16.481
Unidentified	4.9	16.296
Unidentified	3.5	9.152
C12:0	3.1	12
C13:0 3OH/C15:1 i I/H	2.9	14.469
C18:0	2.3	18
Unidentified	2.1	14.28
C16:1 ω9c	1.9	15.774
C15:0 ANTEISO	1.8	14.711
C15:0 ISO 3OH	1.6	16.135
Unidentified	1.5	18.098
Unidentified	1	14.948
Unidentified	1	18.057
C13:0 ISO 2OH	0.9	13.814
C18:1 ω7c /12t/9t	0.9	17.824
C16:0 3OH	0.9	17.52
C15:0	0.7	15
C15:1 ω8c	0.7	14.792
C13:1 at 12-13	0.6	12.931
Unidentified	0.5	13.766
C16:1 ω5c	0.5	15.908
C13:0	0.5	13
Unidentified	0.4	12.262
C17:1 ω8c	0.4	16.792
Unidentified	0.3	9.438
C14:1 ω5c	0.1	13.899

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #8249]	12985	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #8249]	12292	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8249]	Sample type/isolated from	bovine liver abscess
[Ref.: #8249]	Country	Japan
[Ref.: #8249]	Country ISO 3 Code	JPN
[Ref.: #8249]	Continent	Asia

[Ref.: #55350]	Sample type/isolated from	Bovine liver abscess

[Ref.: #67770]	Sample type/isolated from	Bovine liver abscess

[Ref.: #121253]	Sample type/isolated from	Animal, Bovine liver abscess
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Other	#Abscess
#Host Body-Site	#Organ	#Liver

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM905356 (>99% sequence identity) for Fusobacterium necrophorum from Microbeatlas

Aquatic Samples	1051
Soil Samples	1769
Animal Samples	26978
Plant Samples	271
Total Samples	30069

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8249]	Pathogenicity (human)	yes
[Ref.: #8249]	Pathogenicity (animal)	yes
[Ref.: #8249]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121253]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8249]	Fusobacterium necrophorum subsp. funduliforme strain ATCC 51357 16S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF342845	746	ENA	143387 tax ID
[Ref.: #8249]	Fusobacterium necrophorum subsp. funduliforme partial 16S rRNA gene, strain DSM 19678T	AM905356	1487	ENA	143387 tax ID
[Ref.: #20218]	Fusobacterium necrophorum subsp. funduliforme gene for 16S ribosomal RNA, paertial sequence, strain: JCM 3724	AB525413	1469	ENA	143387 tax ID	*
[Ref.: #20218]	Fusobacterium necrophorum subsp. funduliforme strain JCM 3724 clone 1 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer and tRNA-Ile gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF369878	537	ENA	143387 tax ID	*
[Ref.: #20218]	Fusobacterium necrophorum subsp. funduliforme strain JCM 3724 clone 2 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF369879	362	ENA	143387 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Fusobacterium necrophorum subsp. funduliforme ATCC 51357	GCA_000262225	contig	GenBank	1095747 tax ID
[Ref.: #67770]	Fusobacterium necrophorum subsp. funduliforme CCUG 42162	GCA_008801775	contig	GenBank	143387 tax ID
[Ref.: #66792]	Fusobacterium necrophorum subsp. funduliforme ATCC 51357	1095747.3	wgs	PATRIC	1095747 tax ID	*
[Ref.: #66792]	Fusobacterium necrophorum subsp. funduliforme strain CCUG 42162	143387.88	wgs	PATRIC	143387 tax ID	*
[Ref.: #66792]	Fusobacterium necrophorum funduliforme ATCC 51357	2537561963	draft	JGI IMG/M	1095747 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Fusobacterium necrophorum subsp. funduliforme ATCC 51357 PATRIC: 1095747.3
[Ref.: #69480]	motile	no	91.939	no
[Ref.: #69480]	flagellated	no	94.732	no
[Ref.: #69480]	gram-positive	no	92.347	no
[Ref.: #69480]	anaerobic	yes	99.999	no
[Ref.: #69480]	aerobic	no	98.579	no
[Ref.: #69480]	halophile	no	75.091	no
[Ref.: #69480]	spore-forming	no	91.212	no
[Ref.: #69480]	glucose-util	yes	81.225	no
[Ref.: #69480]	thermophile	no	97.272	no
[Ref.: #69480]	glucose-ferment	yes	51.6	no

Availability in culture collections External links

[Ref.: #8249]

Culture collection no.

DSM 19678, ATCC 51357, CCUG 42162, CIP 104859, JCM 3724, VPI 6161

[Ref.: #75255]

SI-ID 46149

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proposal of two subspecies of Fusobacterium necrophorum (Flugge) Moore and Holdeman: Fusobacterium necrophorum subsp. necrophorum subsp. nov., nom. rev. (ex Flugge 1886), and Fusobacterium necrophorum subsp. funduliforme subsp. nov., nom. rev. (ex Halle 1898).	Shinjo T, Fujisawa T, Mitsuoka T	Int J Syst Bacteriol	10.1099/00207713-41-3-395	1991	*

References

#8249

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19678

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41348 ; Curators of the CIP;
#55350 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 42162

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#75255

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID46149.1 )

#121253 Collection of Institut Pasteur ; Curators of the CIP; CIP 104859
* These data were automatically processed and therefore are not curated

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Fusobacterium necrophorum subsp. funduliforme ATCC 51357

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