Fusobacterium nucleatum (DSM 19507, ATCC 49256, CIP 104988)

Name: Fusobacterium nucleatum (DSM 19507, ATCC 49256, CIP 104988)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5775

Type strain

Strain Designation EM48

Culture col. no. DSM 19507 ATCC 49256 CIP 104988 JCM 11023 CCUG 37843 1 more

NCBI tax ID(s) 209882 155615

Special Collections DSMZ CCUG JCM CIP

History <- JCM; JCM 11023 <- CIP; CIP 104988 <- ATCC; ATCC 49256 <- J. L. Dzink; EM48 CIP 104988 <-- ATCC 49256 <-- J. L. Dzink EM48. CIP <- 1996, ATCC <- J.L. Dzink: strain EM48

Links

Fusobacterium nucleatum EM48 is an anaerobe, Gram-negative, rod-shaped bacterium that was isolated from human, periodontal pocket.

Gram-negative rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 42385

ATCC 49256,CCUG 37843,CIP 104988,JCM 11023,KCTC 5105,DSM 19507

@ref 20215

Last LPSN update 2026-02-09 11:19:03

Domain Bacteria

Phylum Fusobacteriota

Class Fusobacteriia

Order Fusobacteriales

Family Fusobacteriaceae

Genus Fusobacterium

Species Fusobacterium nucleatum

Full scientific name Fusobacterium nucleatum Knorr 1922 (Approved Lists 1980)

Synonyms (6)

Fusobacterium nucleatum subsp. fusiforme
"Fusobacterium fusiforme"
Fusobacterium nucleatum subsp. vincentii
Fusobacterium vincentii
Fusobacterium animalis
Fusobacterium nucleatum subsp. animalis

BacDive ID	Other strains from **Fusobacterium nucleatum** (9)	Type strain
142753	F. nucleatum CCUG 14448
142924	F. nucleatum CCUG 15725
142925	F. nucleatum CCUG 15758
151510	F. nucleatum CCUG 44169
152134	F. nucleatum CCUG 45873
154184	F. nucleatum CCUG 52678
154363	F. nucleatum CCUG 53249
155322	F. nucleatum CCUG 57321
156450	F. nucleatum CCUG 61856

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
121370	negative	rod-shaped

Colony morphology

@ref	Incubation period
8137	1-2 days
53908	2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8137	CHOPPED MEAT MEDIUM (DSMZ Medium 78)	Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78; with strain-specific modifications) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) Yeast extract 5.0 g/l K2HPO4 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
8137	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
41412	MEDIUM 20 - for Anaerobic bacteria		Agar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
121370	CIP Medium 20	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8137	positive	growth	37
41412	positive	growth	37
53908	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
8137	anaerobe
53908	anaerobe
121370	anaerobe
125438	anaerobe	94.896

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
68367	17057 ChEBI	cellobiose	-	builds acid from	from API 20A
68367	17634 ChEBI	D-glucose	-	builds acid from	from API 20A
68367	16899 ChEBI	D-mannitol	-	builds acid from	from API 20A
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
68367	16024 ChEBI	D-mannose	-	builds acid from	from API 20A
68367	65327 ChEBI	D-xylose	-	builds acid from	from API 20A
68367	4853 ChEBI	esculin	+	hydrolysis	from API 20A
68367	5291 ChEBI	gelatin	-	hydrolysis	from API 20A
68367	17754 ChEBI	glycerol	-	builds acid from	from API 20A
68367	30849 ChEBI	L-arabinose	-	builds acid from	from API 20A
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68367	62345 ChEBI	L-rhamnose	-	builds acid from	from API 20A
68367	17716 ChEBI	lactose	-	builds acid from	from API 20A
68367	17306 ChEBI	maltose	-	builds acid from	from API 20A
68367	6731 ChEBI	melezitose	-	builds acid from	from API 20A
121370	17632 ChEBI	nitrate	+	reduction
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
121370	16301 ChEBI	nitrite	+	reduction
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
68367	16634 ChEBI	raffinose	-	builds acid from	from API 20A
68367	17814 ChEBI	salicin	-	builds acid from	from API 20A
68367	30911 ChEBI	sorbitol	-	builds acid from	from API 20A
68367	17992 ChEBI	sucrose	-	builds acid from	from API 20A
68367	27082 ChEBI	trehalose	-	builds acid from	from API 20A
68380	27897 ChEBI	tryptophan	+	energy source	from API rID32A
68367	27897 ChEBI	tryptophan	+	energy source	from API 20A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
68367	16199 ChEBI	urea	-	hydrolysis	from API 20A

Metabolite production

@ref	Chebi-ID	Metabolite
68380	35581 ChEBI	indole	from API rID32A
121370	35581 ChEBI	indole
68367	35581 ChEBI	indole	from API 20A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	+	from API rID32A
68367	35581 ChEBI	indole	+	from API 20A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68380	alpha-galactosidase	-	3.2.1.22	from API rID32A
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68380	alpha-glucosidase	-	3.2.1.20	from API rID32A
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68382	beta-galactosidase	-	3.2.1.23	from API zym
68380	beta-galactosidase	-	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68382	beta-glucosidase	-	3.2.1.21	from API zym
68380	beta-glucosidase	-	3.2.1.21	from API rID32A
68367	beta-glucosidase	+	3.2.1.21	from API 20A
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
121370	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68367	gelatinase	-		from API 20A
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
68382	leucine arylamidase	-	3.4.11.1	from API zym
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121370	oxidase	-
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	+	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	-		from API rID32A
121370	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
68367	urease	-	3.5.1.5	from API 20A
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121370	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 20A

@ref	IND	URE	GLU	MAN	LAC	SAC	MAL	SAL	XYL	ARA	GEL	ESC	GLY	CEL	MNE	MLZ	RAF	SOR	RHA	TRE	CAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
8137	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
8137	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-
8137	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Periodontal pocket

Isolation

@ref	Sample type
8137	human, periodontal pocket
53908	Human periodontal pocket
67770	Human oral cavity
121370	Human, Periodontal pocket

Taxonmaps

69479

Global distribution of 16S sequence AM887529 (>99% sequence identity) for Fusobacterium from Microbeatlas

Aquatic Samples	32
Soil Samples	16
Animal Samples	3941
Plant Samples	15
Total Samples	4004

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8137	2	Risk group (German classification) According to TRBA 466
121370	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID
67770	ASM18294v1 assembly for Fusobacterium vincentii ATCC 49256	contig	GCA_000182945	209882.4	638341097	209882

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Fusobacterium nucleatum subsp. vincentii ATCC 49256 16S ribosomal RNA, partial	AJ006964	1406	209882
20218	Fusobacterium nucleatum subsp. vincentii partial 16S rRNA gene, type strain DSM 19507T	AM887529	1431	155615
20218	Fusobacterium nucleatum subsp. vincentii strain ATCC 49256 16S ribosomal RNA gene, partial sequence	GQ301040	1415	209882
20218	Fusobacterium nucleatum subsp. vincentii ATCC 49256 16S ribosomal RNA gene, partial sequence	HM347072	1399	209882
8137	Fusobacterium nucleatum subsp. vincentii gene for 16S ribosomal RNA, partial sequence, strain: JCM 11023	AB971819	1480	155615
8137	Fusobacterium nucleatum subsp. vincentii strain ATCC 49256 16S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF342834	729	209882

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Fusobacterium vincentii ATCC 49256
NCBI: GCA_000182945

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	68.30	no
125439	motility	BacteriaNetⓘ	yes	59.10	no
125439	gram_stain	BacteriaNetⓘ	positive	65.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	88.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Fusobacterium nucleatum subsp. vincentii ATCC 49256
PATRIC: 209882.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	86.03	no
125438	anaerobic	anaerobicⓘ	yes	94.90	yes
125438	spore-forming	spore-formingⓘ	no	84.93	no
125438	aerobic	aerobicⓘ	no	98.84	yes
125438	thermophilic	thermophileⓘ	no	93.73	no
125438	flagellated	motile2+ⓘ	no	84.88	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Draft Genome Sequence of Fusobacterium vincentii CNGBCC1850030, Isolated from Healthy Human Feces.	Jing X, Liu C, Ye Y, Xu J, Huang H, Wang B, Wei J, Zhao J.	Microbiol Resour Announc	10.1128/mra.00543-22	2023
Phylogeny	The rise and evolving role of Fusobacterium nucleatum subspecies.	Wolf M, Steinberg T, Scholz KJ, Kruse A, Rezasoltani S, Conrads G, Al-Ahmad A, Cieplik F.	Curr Res Microb Sci	10.1016/j.crmicr.2025.100414	2025
Phylogeny	Development of strain-specific PCR primers for the identification of Fusobacterium nucleatum subsp. fusiforme ATCC 51190(T) and subsp. vincentii ATCC 49256(T).	Shin HS, Kim MJ, Kim HS, Park SN, Kim DK, Baek DH, Kim C, Kook JK.	Anaerobe	10.1016/j.anaerobe.2009.04.003	2010
Pathogenicity	Fusobacterium spp. target human CEACAM1 via the trimeric autotransporter adhesin CbpF.	Brewer ML, Dymock D, Brady RL, Singer BB, Virji M, Hill DJ.	J Oral Microbiol	10.1080/20002297.2018.1565043	2019
	Clostridium butyricum-altered lung microbiome is associated with enhanced anti-influenza effects via G-protein-coupled receptor120.	Hagihara M, Yamashita M, Ariyoshi T, Minemura A, Yoshida C, Higashi S, Oka K, Takahashi M, Ota A, Maenaka A, Iwasaki K, Hirai J, Shibata Y, Umemura T, Mori T, Kato H, Asai N, Mikamo H.	iScience	10.1016/j.isci.2025.113502	2025
Genetics	Noncontiguous finished genome sequence and description of Fusobacterium massiliense sp. nov. isolated from human duodenum.	Mailhe M, Ricaboni D, Vitton V, Benezech A, Dubourg G, Michelle C, Andrieu C, Armstrong N, Bittar F, Fournier PE, Raoult D, Million M.	New Microbes New Infect	10.1016/j.nmni.2016.12.003	2017
Phylogeny	Proposal of three subspecies of Fusobacterium nucleatum Knorr 1922: Fusobacterium nucleatum subsp. nucleatum subsp. nov., comb. nov.; Fusobacterium nucleatum subsp. polymorphum subsp. nov., nom. rev., comb. nov.; and Fusobacterium nucleatum subsp. vincentii subsp. nov., nom. rev., comb. nov.	Dzink JL, Sheenan MT, Socransky SS	Int J Syst Bacteriol	10.1099/00207713-40-1-74	1990

References

#8137	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19507
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#41412	; Curators of the CIP;
#53908	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37843
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68367	Automatically annotated from API 20A .
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121370	Collection of Institut Pasteur ; Curators of the CIP; CIP 104988
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Fusobacterium nucleatum (DSM 19507, ATCC 49256, CIP 104988)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20A

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000182945

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media