Chryseobacterium joostei (DSM 16927, CCM 7154, CCUG 46665)

Name: Chryseobacterium joostei (DSM 16927, CCM 7154, CCUG 46665)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5674

Type strain

Strain Designation Ix 5a

Culture col. no. DSM 16927 CCM 7154 CCUG 46665 Ix 5a LMG 18212 1 more

NCBI tax ID(s) 112234

Special Collections DSMZ CCUG CIP

History DSMZ <- CCUG <- LMG <- C. Hugo University of the Orange Free State Bloemfontein South Africa <- P. J. Jooste CIP <- 1998, J.F. Bernardet, INRA, Jouy-en-Josas, France <- C.J. Hugo: strain Ix 5a <- P.J. Jooste

Links

Chryseobacterium joostei Ix 5a is an aerobe, Gram-negative, rod-shaped animal pathogen that was isolated from raw milk, tanker.

Gram-negative rod-shaped aerobe animal pathogen genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 11460

LMG 18212,CCUG 46665,CCM 7154,CIP 105533,BCRC 17367,CCRC 17367,KCTC 12128,DSM 16927

@ref 20215

Last LPSN update 2026-05-29 08:26:13

Domain Bacteria

Phylum Bacteroidota

Class Flavobacteriia

Order Flavobacteriales

Family Weeksellaceae

Genus Chryseobacterium

Species Chryseobacterium joostei

Full scientific name Chryseobacterium joostei Hugo et al. 2003

BacDive ID	Other strains from **Chryseobacterium joostei** (2)	Type strain
136960	C. joostei UOF CR694, CIP 108621, LMG 22906
136961	C. joostei UOF CR1094, CIP 108622, LMG 22907

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120362	negative	rod-shaped
125439	negative		99.554
125438			92
125438	negative		97.455

Colony morphology

120362

Hemolysis ability0

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6648	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
6648	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
6648	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
38560	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
120362	CIP Medium 3	Medium recipe at CIP
120362	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6648	positive	growth	30
6648	positive	growth	28
38560	positive	growth	25
57298	positive	growth	28
120362	positive	growth	5-37
120362	negative	growth	41
120362	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
57298	aerobe
120362	obligate aerobe
125439	obligate aerobe	94.748

Spore formation

@ref	Spore formation	Confidence
125439		99.587

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120362	NaCl	positive	growth	0-2 %
120362	NaCl		growth	4 %
120362	NaCl		growth	6 %
120362	NaCl		growth	8 %
120362	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
120362	16947 ChEBI	citrate	-	carbon source
120362	4853 ChEBI	esculin	+	hydrolysis
120362	17234 ChEBI	glucose	+	degradation
120362	606565 ChEBI	hippurate	-	hydrolysis
120362	15792 ChEBI	malonate	-	assimilation
120362	17632 ChEBI	nitrate	-	builds gas from
120362	17632 ChEBI	nitrate	-	reduction
120362	16301 ChEBI	nitrite	-	builds gas from
120362	16301 ChEBI	nitrite	-	reduction

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
120362	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120362	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
120362	15688 ChEBI	acetoin	-
120362	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120362	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120362	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
120362	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120362	caseinase	+	3.4.21.50
120362	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
120362	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120362	gamma-glutamyltransferase	+	2.3.2.2
120362	gelatinase	+
120362	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
120362	lipase	-
68382	lipase (C 14)	-		from API zym
120362	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120362	ornithine decarboxylase	-	4.1.1.17
120362	oxidase	+
120362	phenylalanine ammonia-lyase	-	4.3.1.24
120362	protease	+
68382	trypsin	+	3.4.21.4	from API zym
120362	tryptophan deaminase	-
120362	tween esterase	-
120362	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120362	-	+	+	+	-	+	+	-	+	-	+	+	-	-	-	+	-	+	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120362	not determinedn.d.	+	+	-	+	+	-	-	-	-	+	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	#Container (Reservoir)
#Host Body Product	#Fluids	#Milk

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
6648	raw milk, tanker		Ixopo Kwazulu-Natal	South Africa	ZAF	Africa
57298	Raw milk,tanker	1981	Ixopo Kwazulu-Natal	South Africa	ZAF	Africa
120362	Food, Cow, milk			South Africa	ZAF	Africa	1981

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
6648		1	Risk group (German classification) According to TRBA 466
120362		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM381577v1 assembly for Chryseobacterium joostei DSM 16927	complete	GCA_003815775			112234	98.12
66792	IMG-taxon 2681813505 annotated assembly for Chryseobacterium joostei DSM 16927	scaffold	GCA_900156585	112234.4	2681813505	112234	70.3

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6648	Chryseobacterium joosteii 16S rRNA gene, strain LMG 18212	AJ271010	1469		112234
124043	Chryseobacterium joostei strain LMG 18212 16S ribosomal RNA gene, partial sequence.	MG846021	1478		112234

GC content

6648

GC-content (mol%)36.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: IMG-taxon 2681813505 annotated assembly for Chryseobacterium joostei DSM 16927
BV-BRC: 112234.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.75	no
125439	gram_stain	BacteriaNetⓘ	negative	99.55	no
125439	motility	BacteriaNetⓘ	no	81.36	no
125439	spore_formation	BacteriaNetⓘ	no	99.59	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Chryseobacterium joostei DSM 16927
NCBI: GCA_003815775.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.46	no
125438	anaerobic	anaerobicⓘ	no	97.78	yes
125438	aerobic	aerobicⓘ	yes	88.22	no
125438	spore-forming	spore-formingⓘ	no	86.67	no
125438	thermophilic	thermophileⓘ	no	97.00	no
125438	flagellated	motile2+ⓘ	no	92.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Mechanistic Characterisation of a Diterpene Synthase for Chryseojoostenes A-E from Chryseobacterium Joostei.	Tabekoueng GB, Li H, Yang K, Lauterbach L, Goldfuss B, Dickschat JS.	Angew Chem Int Ed Engl	10.1002/anie.202513149	2025
Genetics	Comparative Genomics Analysis of Keratin-Degrading Chryseobacterium Species Reveals Their Keratinolytic Potential for Secondary Metabolite Production.	Kang D, Shoaie S, Jacquiod S, Sorensen SJ, Ledesma-Amaro R.	Microorganisms	10.3390/microorganisms9051042	2021
	Diversity, distribution, and antagonistic activities of rhizobacteria of Panax notoginseng.	Fan ZY, Miao CP, Qiao XG, Zheng YK, Chen HH, Chen YW, Xu LH, Zhao LX, Guan HL.	J Ginseng Res	10.1016/j.jgr.2015.05.003	2016
Metabolism	Interaction between selected bacterial strains and Arabidopsis halleri modulates shoot proteome and cadmium and zinc accumulation.	Farinati S, DalCrso G, Panigati M, Furini A.	J Exp Bot	10.1093/jxb/err015	2011
Phylogeny	Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria.	Mellmann A, Cloud J, Maier T, Keckevoet U, Ramminger I, Iwen P, Dunn J, Hall G, Wilson D, Lasala P, Kostrzewa M, Harmsen D.	J Clin Microbiol	10.1128/jcm.00157-08	2008
Phylogeny	Chryseobacterium panacisoli sp. nov., isolated from ginseng-cultivation soil with ginsenoside-converting activity.	Keum DH, Yeon JM, Yun CS, Lee SY, Im WT	Int J Syst Evol Microbiol	10.1099/ijsem.0.005086	2021
Phylogeny	Chryseobacterium artocarpi sp. nov., isolated from the rhizosphere soil of Artocarpus integer.	Venil CK, Nordin N, Zakaria ZA, Ahmad WA	Int J Syst Evol Microbiol	10.1099/ijs.0.063594-0	2014
Phylogeny	Chryseobacterium joostei sp. nov., isolated from the dairy environment.	Hugo CJ, Segers P, Hoste B, Vancanneyt M, Kersters K	Int J Syst Evol Microbiol	10.1099/ijs.0.02232-0	2003

References

#6648	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16927
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#38560	; Curators of the CIP;
#57298	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 46665
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#120362	Collection of Institut Pasteur ; Curators of the CIP; CIP 105533
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Chryseobacterium joostei (DSM 16927, CCM 7154, CCUG 46665)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003815775

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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