Name: Robiginitalea biformata DSM 15991
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5636

Type strain:

Species: Robiginitalea biformata

Culture col. no.: DSM 15991, HTCC 2501, KCTC 12146

Strain history: <- J.-C. Cho

NCBI tax ID(s): 252307 (species)

SI-ID 127233

ATCC BAA-864, KCTC 12146, DSM 15991

Other strains from Robiginitalea biformata

R. biformata DSM 15992, HTCC 2514

Section

Robiginitalea biformata DSM 15991 is an aerobe, chemoheterotroph, mesophilic bacterium that forms circular colonies and was isolated from seawater.

colony-forming
Gram-negative
rod-shaped
aerobe
chemoheterotroph
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Robiginitalea*
	Species	**Robiginitalea biformata**
	Full Scientific Name (LPSN)	Robiginitalea biformata Cho and Giovannoni 2004

Information on morphological and physiological properties Morphology

[Ref.: #30171]	Gram stain	negative
[Ref.: #26520]	Gram stain	negative

[Ref.: #30171]	Cell length	3.6 µm
[Ref.: #26520]	Cell length	1.6-5.6 µm

[Ref.: #30171]	Cell width	0.5 µm
[Ref.: #26520]	Cell width	0.3-0.7 µm

[Ref.: #30171]	Cell shape	rod-shaped
[Ref.: #26520]	Cell shape	rod-shaped

[Ref.: #30171]	Motility	no
[Ref.: #26520]	Motility	no

[Ref.: #26520]	Colony color	rusty-orange-colored
[Ref.: #26520]	Colony shape	circular

[Ref.: #30171]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6225]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6225]

Culture medium growth

[Ref.: #6225]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6225]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6225]	growth	30 °C
[Ref.: #26520]	growth	10-44 °C
[Ref.: #30171]	growth	10-44 °C
[Ref.: #30171]	optimum	27 °C

[Ref.: #6225]	Temperature range	mesophilic
[Ref.: #30171]	Temperature range	mesophilic

		pH
[Ref.: #30171]	optimum	7.5
[Ref.: #30171]	growth	6.0-9.0
[Ref.: #26520]	growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30171]	Oxygen tolerance	aerobe
[Ref.: #26520]	Oxygen tolerance	aerobe

[Ref.: #26520]

Nutrition type

chemoheterotroph

[Ref.: #30171]	Ability of spore formation	no
[Ref.: #26520]	Ability of spore formation	no

[Ref.: #26520]

Halophily / tolerance level

moderately halophilic

	Salt		Salt conc.
[Ref.: #30171]	NaCl	growth	0.25-10	%
[Ref.: #30171]	NaCl	optimum	5.1	%
[Ref.: #26520]	NaCl	growth	0.25-10	%(w/v)

[Ref.: #26520]

Observation

formation of poly-beta-hydroxybutyrate granules

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #26520]	alginate ChEBI	-	degradation
[Ref.: #26520]	beta-lactose ChEBI	+	carbon source
[Ref.: #26520]	carrageenan ChEBI	-	degradation
[Ref.: #26520]	casein	-	degradation
[Ref.: #30171]	cellobiose ChEBI	+	carbon source
[Ref.: #26520]	cellobiose ChEBI	+	carbon source
[Ref.: #26520]	cellulose ChEBI	-	degradation
[Ref.: #26520]	chitin ChEBI	-	degradation
[Ref.: #26520]	citrate ChEBI	-	carbon source
[Ref.: #26520]	D-arabinose ChEBI	-	carbon source
[Ref.: #26520]	D-fructose ChEBI	+	carbon source
[Ref.: #26520]	D-galactose ChEBI	-	carbon source
[Ref.: #26520]	D-gluconate ChEBI	-	carbon source
[Ref.: #26520]	D-glucosamine ChEBI	-	carbon source
[Ref.: #26520]	D-glucose ChEBI	+	carbon source
[Ref.: #26520]	D-glyceraldehyde ChEBI	-	carbon source
[Ref.: #26520]	D-malate ChEBI	-	carbon source
[Ref.: #26520]	D-mannitol ChEBI	+/-	carbon source
[Ref.: #26520]	D-mannose ChEBI	+	carbon source
[Ref.: #26520]	D-ribose ChEBI	+	carbon source
[Ref.: #26520]	D-sorbitol ChEBI	-	carbon source
[Ref.: #26520]	D-trehalose ChEBI	+	carbon source
[Ref.: #26520]	D-xylose ChEBI	+	carbon source
[Ref.: #26520]	dextran ChEBI	-	degradation
[Ref.: #26520]	dna ChEBI	-	degradation
[Ref.: #26520]	elastin ChEBI	-	degradation
[Ref.: #30171]	esculin ChEBI	+	hydrolysis
[Ref.: #26520]	esculin ChEBI	-	degradation
[Ref.: #26520]	esculin ChEBI	+	hydrolysis
[Ref.: #26520]	ethanol ChEBI	-	carbon source
[Ref.: #26520]	formate ChEBI	-	carbon source
[Ref.: #30171]	fructose ChEBI	+	carbon source
[Ref.: #26520]	gelatin ChEBI	-	degradation
[Ref.: #30171]	glucose ChEBI	+	carbon source
[Ref.: #26520]	glucose ChEBI	-	builds acid from
[Ref.: #30171]	glutamate ChEBI	+	carbon source
[Ref.: #26520]	glycerol ChEBI	-	carbon source
[Ref.: #26520]	glycine ChEBI	-	carbon source
[Ref.: #26520]	itaconate ChEBI	-	carbon source
[Ref.: #26520]	L-alanine ChEBI	-	carbon source
[Ref.: #26520]	L-arginine ChEBI	-	carbon source
[Ref.: #26520]	L-glutamate ChEBI	+	carbon source
[Ref.: #26520]	L-isoleucine ChEBI	-	carbon source
[Ref.: #26520]	L-leucine ChEBI	-	carbon source
[Ref.: #26520]	L-lysine ChEBI	-	carbon source
[Ref.: #26520]	L-ornithine ChEBI	+	carbon source
[Ref.: #26520]	L-proline ChEBI	+	carbon source
[Ref.: #26520]	L-rhamnose ChEBI	-	carbon source
[Ref.: #26520]	L-serine ChEBI	+	carbon source
[Ref.: #26520]	L-sorbose ChEBI	+	carbon source
[Ref.: #30171]	lactate ChEBI	+	carbon source
[Ref.: #26520]	lactate ChEBI	+	carbon source
[Ref.: #30171]	lactose ChEBI	+	carbon source
[Ref.: #26520]	malonate ChEBI	-	carbon source
[Ref.: #30171]	maltose ChEBI	+	carbon source
[Ref.: #26520]	maltose ChEBI	+	carbon source
[Ref.: #30171]	mannose ChEBI	+	carbon source
[Ref.: #26520]	melezitose ChEBI	+/-	carbon source
[Ref.: #30171]	melibiose ChEBI	+	carbon source
[Ref.: #26520]	melibiose ChEBI	+	carbon source
[Ref.: #26520]	methanol ChEBI	-	carbon source
[Ref.: #26520]	myo-inositol ChEBI	+/-	carbon source
[Ref.: #30171]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #26520]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #26520]	nitrate ChEBI	-	reduction
[Ref.: #30171]	ornithine ChEBI	+	carbon source
[Ref.: #30171]	proline ChEBI	+	carbon source
[Ref.: #30171]	propionate ChEBI	+	carbon source
[Ref.: #26520]	propionate ChEBI	+	carbon source
[Ref.: #26520]	pyruvate ChEBI	-	carbon source
[Ref.: #30171]	raffinose ChEBI	+	carbon source
[Ref.: #26520]	raffinose ChEBI	+	carbon source
[Ref.: #26520]	ribitol ChEBI	-	carbon source
[Ref.: #30171]	ribose ChEBI	+	carbon source
[Ref.: #30171]	serine ChEBI	+	carbon source
[Ref.: #26520]	starch ChEBI	+	degradation
[Ref.: #30171]	succinate ChEBI	+	carbon source
[Ref.: #26520]	succinate ChEBI	+	carbon source
[Ref.: #30171]	sucrose ChEBI	+	carbon source
[Ref.: #26520]	sucrose ChEBI	+	carbon source
[Ref.: #30171]	trehalose ChEBI	+	carbon source
[Ref.: #30171]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #26520]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #26520]	arginine dihydrolase	-	3.5.3.6
[Ref.: #26520]	beta-galactosidase	+	3.2.1.23
[Ref.: #30171]	catalase	+	1.11.1.6
[Ref.: #26520]	catalase	+	1.11.1.6
[Ref.: #30171]	cytochrome oxidase	+	1.9.3.1
[Ref.: #26520]	cytochrome oxidase	+	1.9.3.1
[Ref.: #26520]	gelatinase	-
[Ref.: #26520]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6225]	Sample type/isolated from	seawater
[Ref.: #6225]	Geographic location (country and/or sea, region)	Sargasso Sea, Atlantic Ocean

[Ref.: #26520]	Sample type/isolated from	marine water sample
[Ref.: #26520]	Geographic location (country and/or sea, region)	Sargossa Sea
[Ref.: #26520]	Country	Atlantic Ocean
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Marine

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence NR_074601 (>99% sequence identity) for Robiginitalea biformata subclade from Microbeatlas

Aquatic Samples	1873
Soil Samples	42
Animal Samples	19
Plant Samples	3
Total Samples	1937

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6225]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6225]	Robiginitalea biformata HTCC2501 16S ribosomal RNA, partial sequence	NR_074601	1518	GenBank	313596 tax ID
[Ref.: #26520]	16S rRNA genes	AY424899		GenBank

[Ref.: #30171]	GC-content	56.4 mol%
[Ref.: #26520]	GC-content	56.2-56.6 mol%	high performance liquid chromatography (HPLC)
[Ref.: #6225]	GC-content	55.3 mol%	sequence analysis

Availability in culture collections External links

[Ref.: #6225]

Culture collection no.

DSM 15991, HTCC 2501, KCTC 12146

[Ref.: #75119]

SI-ID 127233

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete genome sequence of Robiginitalea biformata HTCC2501.	Oh HM, Giovannoni SJ, Lee K, Ferriera S, Johnson J, Cho JC	J Bacteriol	10.1128/JB.01191-09	2009	*
Phylogeny	Robiginitalea myxolifaciens sp. nov., a novel myxol-producing bacterium isolated from marine sediment, and emended description of the genus Robiginitalea.	Manh HD, Matsuo Y, Katsuta A, Matsuda S, Shizuri Y, Kasai H	Int J Syst Evol Microbiol	10.1099/ijs.0.65598-0	2008	*
Phylogeny	Robiginitalea biformata gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae with a higher G+C content.	Cho JC, Giovannoni SJ	Int J Syst Evol Microbiol	10.1099/ijs.0.03023-0	2004	*

References

#6225

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15991

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#26520

IJSEM 1101 2004 ( DOI 10.1099/ijs.0.03023-0 , PubMed 15280276 )

#30171

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26520 (see below)

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#75119

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID127233.1 )

#26520

IJSEM 1101 2004 ( DOI 10.1099/ijs.0.03023-0 , PubMed 15280276 )

* These data were automatically processed and therefore are not curated

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