Name: Anoxybacillus tepidamans GS5-97
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 556

Type strain:

Species: Anoxybacillus tepidamans

Strain Designation: GS5-97, R-35643

Culture col. no.: DSM 16325, ATCC BAA-942

Strain history: <- P. Messner; GS5-97 <- M. Graninger and A. Scheberl <- F. Hollaus

NCBI tax ID(s): 265948 (species)

SI-ID 134425

ATCC BAA-942, DSM 16325, LMG23036, KACC 11371, LMG 26208

Section

Anoxybacillus tepidamans GS5-97 is an aerobe, spore-forming, thermophilic bacterium that was isolated from extraction juice samples.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Anoxybacillus*
	Species	**Anoxybacillus tepidamans**
	Full Scientific Name (LPSN)	Anoxybacillus tepidamans (Schäffer et al. 2004) Coorevits et al. 2012

Synonym

Geobacillus tepidamans

Information on morphological and physiological properties Morphology

[Ref.: #31291]	Gram stain	positive

[Ref.: #31291]	Cell length	4.3 µm

[Ref.: #31291]	Cell width	1.05 µm

[Ref.: #31291]	Cell shape	rod-shaped

[Ref.: #31291]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6367]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #6367]

Culture medium growth

[Ref.: #6367]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6367]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #6367]			growth	55 °C
[Ref.: #31291]			optimum	55 °C

[Ref.: #6367]	Temperature range	thermophilic
[Ref.: #31291]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #31291]			optimum	7
[Ref.: #31291]			growth	06-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31291]

Oxygen tolerance

aerobe

[Ref.: #31291]

Ability of spore formation

yes

[Ref.: #31291]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31291]	fructose ChEBI	+	carbon source
[Ref.: #31291]	galactose ChEBI	+	carbon source
[Ref.: #31291]	glucose ChEBI	+	carbon source
[Ref.: #31291]	lactose ChEBI	+	carbon source
[Ref.: #31291]	maltose ChEBI	+	carbon source
[Ref.: #31291]	mannose ChEBI	+	carbon source
[Ref.: #31291]	nitrate ChEBI	+	reduction
[Ref.: #31291]	sucrose ChEBI	+	carbon source
[Ref.: #31291]	trehalose ChEBI	+	carbon source
[Ref.: #31291]	xylose ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #31291]		catalase	+	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6367]	Sample type/isolated from	extraction juice samples
[Ref.: #6367]	Geographic location (country and/or sea, region)	Beet Sugar Factory, Leopoldsdorf, lower Austria
[Ref.: #6367]	Country	Austria
[Ref.: #6367]	Country ISO 3 Code	AUT
[Ref.: #6367]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Beverage
#Host Body Product	#Plant	#Juice (natural)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FN428691 (>99% sequence identity) for Anoxybacillus tepidamans from Microbeatlas

Aquatic Samples	44
Soil Samples	52
Animal Samples	173
Plant Samples	6
Total Samples	275

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6367]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6367]	Geobacillus tepidamans partial 16S rRNA gene, strain R-35643	FN428691	1507	ENA	265948 tax ID
[Ref.: #6367]	Geobacillus tepidamans strain GS5-97 16S ribosomal RNA gene, partial sequence	AY563003	1447	ENA	265948 tax ID
[Ref.: #20218]	Geobacillus tepidamans strain DSM 16325 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU157951	237	ENA	265948 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Anoxybacillus tepidamans DSM 16325	GCA_014201465	scaffold	GenBank	265948 tax ID	*
[Ref.: #66792]	Anoxybacillus tepidamans strain DSM 16325	265948.4	wgs	PATRIC	265948 tax ID	*
[Ref.: #66792]	Anoxybacillus tepidamans DSM 16325	2861206899	draft	JGI IMG/M	265948 tax ID	*

[Ref.: #6367]

GC-content

43.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Anoxybacillus tepidamans DSM 16325 NCBI: GCA_014201465.1
[Ref.: #69480]	flagellated	yes	84.177	no
[Ref.: #69480]	gram-positive	yes	88.335	yes
[Ref.: #69480]	anaerobic	no	97.659	yes
[Ref.: #69480]	aerobic	yes	75.499	no
[Ref.: #69480]	halophile	no	94.848	no
[Ref.: #69480]	spore-forming	yes	94.364	no
[Ref.: #69480]	glucose-util	yes	90.358	yes
[Ref.: #69480]	thermophile	yes	99.107	yes
[Ref.: #69480]	motile	yes	92.682	yes
[Ref.: #69480]	glucose-ferment	no	88.482	no

Availability in culture collections External links

[Ref.: #6367]

Culture collection no.

DSM 16325, ATCC BAA-942

[Ref.: #70234]

SI-ID 134425

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Anoxybacillusgeothermalis sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station.	Filippidou S, Jaussi M, Junier T, Wunderlin T, Jeanneret N, Palmieri F, Palmieri I, Roussel-Delif L, Vieth-Hillebrand A, Vetter A, Chain PS, Regenspurg S, Junier P	Int J Syst Evol Microbiol	10.1099/ijsem.0.001125	2016	*
Phylogeny	Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov.	Coorevits A, Dinsdale AE, Halket G, Lebbe L, De Vos P, Van Landschoot A, Logan NA	Int J Syst Evol Microbiol	10.1099/ijs.0.030346-0	2011	*
Enzymology	The dTDP-4-dehydro-6-deoxyglucose reductase encoding fcd gene is part of the surface layer glycoprotein glycosylation gene cluster of Geobacillus tepidamans GS5-97T.	Zayni S, Steiner K, Pfostl A, Hofinger A, Kosma P, Schaffer C, Messner P	Glycobiology	10.1093/glycob/cwl084	2007	*
Genetics	The secondary cell wall polymer of Geobacillus tepidamans GS5-97T: structure of different glycoforms.	Steindl C, Schaffer C, Smrecki V, Messner P, Muller N	Carbohydr Res	10.1016/j.carres.2005.07.005	2005	*
Enzymology	N-acetylmuramic acid as capping element of alpha-D-fucose-containing S-layer glycoprotein glycans from Geobacillus tepidamans GS5-97T.	Kahlig H, Kolarich D, Zayni S, Scheberl A, Kosma P, Schaffer C, Messner P	J Biol Chem	10.1074/jbc.M501724200	2005	*
Phylogeny	Classification of isolates from locations in Austria and Yellowstone National Park as Geobacillus tepidamans sp. nov.	Schaffer C, Franck WL, Scheberl A, Kosma P, McDermott TR, Messner P	Int J Syst Evol Microbiol	10.1099/ijs.0.63227-0	2004	*

References

#6367

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16325

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31291

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27610 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#70234

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID134425.1 )

#27610

IJSEM 2361 2004 ( DOI 10.1099/ijs.0.63227-0 , PubMed 15545484 )

* These data were automatically processed and therefore are not curated

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Anoxybacillus tepidamans DSM 16325

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