Flavobacterium columnare 1-S-2cl is a motile, rod-shaped animal pathogen that forms rhizoid colonies and was isolated from Kidney of diseased salmon.
motile rod-shaped colony-forming animal pathogen genome sequence 16S sequence Bacteria| @ref 20215 |
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| Domain Bacteria |
| Phylum Bacteroidota |
| Class Flavobacteriia |
| Order Flavobacteriales |
| Family Flavobacteriaceae |
| Genus Flavobacterium |
| Species Flavobacterium columnare |
| Full scientific name Flavobacterium columnare (Bernardet and Grimont 1989 ex Davis 1922) Bernardet et al. 1996 |
| Synonyms (2) |
| @ref | Colony color | Colony shape | Medium used | |
|---|---|---|---|---|
| 43343 | greenish-yellow | rhizoid | Anacker and Ordal's agar |
| @ref | Production | Name | |
|---|---|---|---|
| 43343 | flexirubin type pigments |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 7633 | R2A MEDIUM (DSMZ Medium 830) | Medium recipe at MediaDive | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water | ||
| 7633 | ENRICHED CYTOPHAGA AGAR MEDIUM (DSMZ Medium 1133) | Medium recipe at MediaDive | Name: ENRICHED CYTOPHAGA AGAR MEDIUM (DSMZ Medium 1133) Composition: Agar 15.0 g/l Tryptone 2.0 g/l Yeast extract 0.5 g/l Beef extract 0.5 g/l Sodium acetate 0.2 g/l Distilled water | ||
| 40693 | MEDIUM 34 - for Flavobacterium branchiophilum and Flavobacterium columnare | Distilled water make up to (1000.000 ml);Agar (10.000 g);Yeast extract (0.500 g);Sodium acetate (0.200 g);Tryptone (0.500 g);Beef extract (0.200 g) | |||
| 43343 | Anacker and Ordal's agar | ||||
| 118709 | CIP Medium 566 | Medium recipe at CIP | |||
| 43343 | Marine agar (MA) | ||||
| 43343 | Tryptic Soy Agar |
| @ref | Oxygen tolerance | Confidence | |
|---|---|---|---|
| 125439 | facultative anaerobe | 92.754 |
| 67770 | Observationquinones: MK-6 |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 43343 | 2509 ChEBI | agar | - | degradation | |
| 43343 | 28938 ChEBI | ammonium | - | nitrogen source | |
| 43343 | 85146 ChEBI | carboxymethylcellulose | - | degradation | |
| 43343 | casein | + | degradation | ||
| 43343 | 17029 ChEBI | chitin | - | degradation | |
| 43343 | 16991 ChEBI | dna | + | degradation | |
| 43343 | 4853 ChEBI | esculin | - | degradation | |
| 118709 | 4853 ChEBI | esculin | - | hydrolysis | |
| 43343 | 5291 ChEBI | gelatin | + | degradation | |
| 43343 | 17234 ChEBI | glucose | - | assimilation | |
| 43343 | 17632 ChEBI | nitrate | - | nitrogen source | |
| 118709 | 17632 ChEBI | nitrate | - | reduction | |
| 118709 | 16301 ChEBI | nitrite | - | reduction | |
| 118709 | 132112 ChEBI | sodium thiosulfate | + | builds gas from | |
| 43343 | 28017 ChEBI | starch | + | degradation | |
| 43343 | 53424 ChEBI | tween 20 | + | degradation | |
| 43343 | 53423 ChEBI | tween 40 | + | degradation | |
| 43343 | 53425 ChEBI | tween 60 | + | degradation | |
| 43343 | 53426 ChEBI | tween 80 | + | degradation | |
| 43343 | 16199 ChEBI | urea | - | degradation |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 118709 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 118709 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 118709 | amylase | - | ||
| 43343 | arginine dihydrolase | - | 3.5.3.6 | |
| 43343 | beta-galactosidase | - | 3.2.1.23 | |
| 118709 | beta-galactosidase | - | 3.2.1.23 | |
| 118709 | caseinase | + | 3.4.21.50 | |
| 43343 | catalase | + | 1.11.1.6 | |
| 118709 | catalase | + | 1.11.1.6 | |
| 43343 | cytochrome oxidase | + | 1.9.3.1 | |
| 118709 | DNase | - | ||
| 118709 | gamma-glutamyltransferase | + | 2.3.2.2 | |
| 118709 | gelatinase | + | ||
| 118709 | lecithinase | - | ||
| 118709 | lipase | - | ||
| 43343 | lysine decarboxylase | - | 4.1.1.18 | |
| 118709 | lysine decarboxylase | - | 4.1.1.18 | |
| 43343 | ornithine decarboxylase | - | 4.1.1.17 | |
| 118709 | ornithine decarboxylase | - | 4.1.1.17 | |
| 118709 | oxidase | - | ||
| 118709 | urease | - | 3.5.1.5 |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Host | #Fishes | #Salmonidae | |
| #Host Body-Site | #Urogenital tract | #Kidney |
| @ref | Sample type | Host species | Geographic location | Country | Country ISO 3 Code | Continent | Isolation date | |
|---|---|---|---|---|---|---|---|---|
| 7633 | Kidney of diseased salmon | Washington, Snake River | USA | USA | North America | |||
| 67770 | Kidney of a diseased chinook salmon (Oncorhynchus tschawytscha) from the Snake River | Oncorhynchus tschawytscha | WA | USA | USA | North America | ||
| 118709 | Animal, Salmon, kidney | Snake river, Washington | United States of America | USA | North America | 1955 |
Global distribution of 16S sequence KC912654 (>99% sequence identity) for Flavobacterium columnare subclade from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM253067v1 assembly for Flavobacterium columnare NBRC 100251 = ATCC 23463 | scaffold | 1218111 | 41.01 | ||||
| 66792 | ASM799083v1 assembly for Flavobacterium columnare NBRC 100251 = ATCC 23463 | contig | 1218111 | 38.66 | ||||
| 124043 | ASM4053885v1 assembly for Flavobacterium columnare DSM 18644 | contig | 996 | 25.96 | ||||
| 124043 | ASM221724v1 assembly for Flavobacterium columnare NBRC 100251 = ATCC 23463 | scaffold | 1218111 | 10.04 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Flavobacterium columnare strain ATCC 23463 16S ribosomal RNA gene, partial sequence | AY577821 | 517 | 996 | ||
| 20218 | Flavobacterium columnare strain ATCC 23463 16S-23S ribosomal RNA intergenic spacer, partial sequence | AY754372 | 576 | 996 | ||
| 20218 | Flavobacterium columnare gene for 16S rRNA, partial sequence, strain: IAM 14301 (= NCMB 2248) | AB010951 | 1478 | 996 | ||
| 20218 | Flavobacterium columnare DNA, 16S-23S intergenic spacer region, complete sequence | AB030748 | 635 | 996 | ||
| 20218 | Flavobacterium columnare gene for 16S rRNA, strain:IFO 15943 | AB078047 | 1471 | 996 | ||
| 20218 | Flavobacterium columnare gene for 16S rRNA, partial sequence, strain: NBRC 100251 | AB681150 | 1441 | 996 | ||
| 20218 | Flavobacterium columnare gene for 16S ribosomal RNA, partial sequence, strain: NCIMB 2248 | D12659 | 1256 | 996 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | facultative anaerobe | 92.75 | no |
| 125439 | gram_stain | BacteriaNetⓘ | negative | 99.60 | no |
| 125439 | motility | BacteriaNetⓘ | yes | 42.27 | no |
| 125439 | spore_formation | BacteriaNetⓘ | no | 99.95 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | no | 97.14 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 96.65 | no |
| 125438 | aerobic | aerobicⓘ | yes | 83.20 | no |
| 125438 | spore-forming | spore-formingⓘ | no | 90.71 | no |
| 125438 | thermophilic | thermophileⓘ | no | 96.12 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 95.50 | yes |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Genetics | Comparative genomic analysis of Flavobacterium species causing columnaris disease of freshwater fish in Thailand: insights into virulence and resistance mechanisms. | Nguyen DHM, Chokmangmeepisarn P, Khianchaikhan K, Morishita M, Uchuwittayakul A, LaFrentz BR, Rodkhum C. | BMC Vet Res | 10.1186/s12917-025-04488-3 | 2025 | |
| Genetics | Genomic evidence of genetic diversity and functional evolution in Flavobacterium columnare. | Han R, Hong Y, Xu R, Guo W, Zhang M, Lu Z, Han Q, Mo Z, Dan X, Li Y. | Front Microbiol | 10.3389/fmicb.2023.1240471 | 2023 | |
| Flavobacterium columnare ferric iron uptake systems are required for virulence. | Conrad RA, Evenhuis JP, Lipscomb RS, Perez-Pascual D, Stevick RJ, Birkett C, Ghigo JM, McBride MJ. | Front Cell Infect Microbiol | 10.3389/fcimb.2022.1029833 | 2022 | ||
| Investigation of the Genus Flavobacterium as a Reservoir for Fish-Pathogenic Bacterial Species: the Case of Flavobacterium collinsii. | Lee BH, Nicolas P, Saticioglu IB, Fradet B, Bernardet JF, Rigaudeau D, Rochat T, Duchaud E. | Appl Environ Microbiol | 10.1128/aem.02162-22 | 2023 | ||
| Phylogeny | Identification of Four Distinct Phylogenetic Groups in Flavobacterium columnare With Fish Host Associations. | LaFrentz BR, Garcia JC, Waldbieser GC, Evenhuis JP, Loch TP, Liles MR, Wong FS, Chang SF. | Front Microbiol | 10.3389/fmicb.2018.00452 | 2018 | |
| Adaptive response to starvation in the fish pathogen Flavobacterium columnare: cell viability and ultrastructural changes. | Arias CR, Lafrentz S, Cai W, Olivares-Fuster O. | BMC Microbiol | 10.1186/1471-2180-12-266 | 2012 | ||
| Enzymology | Simultaneous discrimination between 15 fish pathogens by using 16S ribosomal DNA PCR and DNA microarrays. | Warsen AE, Krug MJ, LaFrentz S, Stanek DR, Loge FJ, Call DR. | Appl Environ Microbiol | 10.1128/aem.70.7.4216-4221.2004 | 2004 | |
| Enzymology | Simultaneous detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, three major fish pathogens, by multiplex PCR. | Del Cerro A, Marquez I, Guijarro JA. | Appl Environ Microbiol | 10.1128/aem.68.10.5177-5180.2002 | 2002 | |
| LolA and LolB are conserved in Bacteroidota and are crucial for gliding motility and Type IX secretion. | De Smet T, Baland E, Giovannercole F, Mignon J, Lizen L, Dugauquier R, Lauber F, Dieu M, Lima-Mendez G, Michaux C, Devos D, Renzi F. | Commun Biol | 10.1038/s42003-025-07817-2 | 2025 | ||
| Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas. | Giordano A, Vella FM, Romano I, Gambacorta A. | J Lipid Res | 10.1194/jlr.m700152-jlr200 | 2007 | ||
| Enzymology | Phage specificity of the freshwater fish pathogen Flavobacterium columnare. | Laanto E, Sundberg LR, Bamford JK. | Appl Environ Microbiol | 10.1128/aem.05574-11 | 2011 | |
| Pathogenicity | Prophages and Past Prophage-Host Interactions Revealed by CRISPR Spacer Content in a Fish Pathogen. | Laanto E, Ravantti JJ, Sundberg LR | Microorganisms | 10.3390/microorganisms8121919 | 2020 | |
| Improving survival and storage stability of bacteria recalcitrant to freeze-drying: a coordinated study by European culture collections. | Peiren J, Buyse J, De Vos P, Lang E, Clermont D, Hamon S, Begaud E, Bizet C, Pascual J, Ruvira MA, Macian MC, Arahal DR | Appl Microbiol Biotechnol | 10.1007/s00253-015-6476-6 | 2015 | ||
| Cultivation | Improved method for determining antibiotic susceptibility of Flavobacterium columnare isolates by broth microdilution. | Darwish AM, Farmer BD, Hawke JP | J Aquat Anim Health | 10.1577/H07-047.1 | 2008 | |
| Phylogeny | Molecular diversity and growth features of Flavobacterium columnare strains isolated in Finland. | Suomalainen LR, Kunttu H, Valtonen ET, Hirvela-Koski V, Tiirola M | Dis Aquat Organ | 10.3354/dao070055 | 2006 | |
| Systemic and mucosal antibody response in tilapia, Oreochromis niloticus (L.), following immunization with Flavobacterium columnare. | Grabowski LD, LaPatra SE, Cain KD | J Fish Dis | 10.1111/j.1365-2761.2004.00576.x | 2004 | ||
| Phylogeny | Caulobacter daechungensis sp. nov., a stalked bacterium isolated from a eutrophic reservoir. | Jin L, Lee HG, Kim HS, Ahn CY, Oh HM. | Int J Syst Evol Microbiol | 10.1099/ijs.0.048884-0 | 2013 | |
| Phylogeny | The fish pathogen Flavobacterium columnare represents four distinct species: Flavobacterium columnare, Flavobacterium covae sp. nov., Flavobacterium davisii sp. nov. and Flavobacterium oreochromis sp. nov., and emended description of Flavobacterium columnare. | LaFrentz BR, Kralova S, Burbick CR, Alexander TL, Phillips CW, Griffin MJ, Waldbieser GC, Garcia JC, de Alexandre Sebastiao F, Soto E, Loch TP, Liles MR, Snekvik KR | Syst Appl Microbiol | 10.1016/j.syapm.2021.126293 | 2021 | |
| Phylogeny | Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov., isolated from greenhouse soil. | Weon HY, Song MH, Son JA, Kim BY, Kwon SW, Go SJ, Stackebrandt E | Int J Syst Evol Microbiol | 10.1099/ijs.0.64935-0 | 2007 | |
| Phylogeny | Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. | Wang ZW, Liu YH, Dai X, Wang BJ, Jiang CY, Liu SJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.64065-0 | 2006 |
| #7633 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18644 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #40693 | ; Curators of the CIP; |
| #43343 | ; 4: 140 - 168 ISBN 978 ( DOI 10.1007/978-0-387-68572-4 ) |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #118709 | Collection of Institut Pasteur ; Curators of the CIP; CIP 103531 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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