Cloacibacterium normanense (DSM 15886, ATCC BAA-825, CCUG 46293)

Name: Cloacibacterium normanense (DSM 15886, ATCC BAA-825, CCUG 46293)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5504

Type strain

Culture col. no. DSM 15886 ATCC BAA-825 CCUG 46293 CIP 108613 NRS 1

NCBI tax ID(s) 237258

Special Collections DSMZ CCUG CIP Literature strains

History DSMZ <- R. S. Tanner; NRS1 <- T. D. Allen CIP <- 2005, CCUG <- P.A. Lawson, UK <- Tanner, USA: strain NRS1

Links

Cloacibacterium normanense DSM 15886 is a facultative anaerobe, Gram-negative, rod-shaped bacterium that was isolated from untreated municipal wastewater.

Gram-negative rod-shaped facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 289357

CCUG 46293,ATCC BAA-825,CIP 108613,DSM 15886,VTT E-082763

@ref 20215

Last LPSN update 2026-05-29 08:26:11

Domain Bacteria

Phylum Bacteroidota

Class Flavobacteriia

Order Flavobacteriales

Family Weeksellaceae

Genus Cloacibacterium

Species Cloacibacterium normanense

Full scientific name Cloacibacterium normanense Allen et al. 2006

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell shape	Confidence
31763	negative	05-27 µm	rod-shaped
121351	negative		rod-shaped
125439	negative			99.663
125438				92
125438	negative			99.476

Colony morphology

121351

Hemolysis ability0

Pigmentation

31763

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6138	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
40491	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121351	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6138	positive	growth	30-37
31763	positive	growth	18-36
31763	positive	optimum	30
40491	positive	growth	37
57139	positive	growth	37
121351	positive	growth	25-37
121351	negative	growth	41

Culture pH

@ref	Ability	Type	PH
31763	positive	optimum	07-08
31763	positive	growth	06-08

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
31763	facultative anaerobe
57139	aerobe
57139	microaerophile
121351	facultative anaerobe
125439	obligate aerobe	98.658

Spore formation

@ref	Spore formation	Confidence
31763
125438		91.803
125439		99.821

Observation

31763

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31763	40585 ChEBI	alpha-cyclodextrin	+	carbon source
121351	15824 ChEBI	D-fructose	-	degradation
121351	17634 ChEBI	D-glucose	+	degradation
121351	16024 ChEBI	D-mannose	+	degradation
31763	4853 ChEBI	esculin	+	hydrolysis
121351	606565 ChEBI	hippurate	+	hydrolysis
121351	17716 ChEBI	lactose	-	degradation
121351	17306 ChEBI	maltose	+	degradation
31763	37684 ChEBI	mannose	+	carbon source
121351	17632 ChEBI	nitrate	-	builds gas from
121351	17632 ChEBI	nitrate	-	reduction
121351	17632 ChEBI	nitrate	+	respiration
121351	16301 ChEBI	nitrite	-	builds gas from
121351	16301 ChEBI	nitrite	-	reduction
121351	17992 ChEBI	sucrose	-	degradation

Metabolite production

@ref	Chebi-ID	Metabolite	Production
31763	35581 ChEBI	indole
121351	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
31763	35581 ChEBI	indole	+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121351	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121351	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
121351	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121351	caseinase	-	3.4.21.50
31763	catalase	+	1.11.1.6
121351	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
31763	cytochrome oxidase	+	1.9.3.1
121351	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121351	gamma-glutamyltransferase	+	2.3.2.2
121351	gelatinase	-
121351	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121351	lipase	-
68382	lipase (C 14)	-		from API zym
121351	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121351	ornithine decarboxylase	-	4.1.1.17
121351	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
121351	tween esterase	-
121351	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	adipate degradation	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	denitrification	100	2 of 2
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	teichoic acid biosynthesis	100	1 of 1
66794	sulfopterin metabolism	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	glycogen metabolism	100	5 of 5
66794	vitamin K metabolism	100	5 of 5
66794	starch degradation	100	10 of 10
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	suberin monomers biosynthesis	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	palmitate biosynthesis	95.45	21 of 22
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	photosynthesis	85.71	12 of 14
66794	glycine betaine biosynthesis	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	cellulose degradation	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	serine metabolism	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	NAD metabolism	77.78	14 of 18
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	leucine metabolism	76.92	10 of 13
66794	isoleucine metabolism	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	purine metabolism	74.47	70 of 94
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	citric acid cycle	71.43	10 of 14
66794	glutamate and glutamine metabolism	71.43	20 of 28
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	phenylalanine metabolism	69.23	9 of 13
66794	histidine metabolism	68.97	20 of 29
66794	pyrimidine metabolism	68.89	31 of 45
66794	acetoin degradation	66.67	2 of 3
66794	cysteine metabolism	66.67	12 of 18
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	flavin biosynthesis	66.67	10 of 15
66794	d-mannose degradation	66.67	6 of 9
66794	formaldehyde oxidation	66.67	2 of 3
66794	alanine metabolism	65.52	19 of 29
66794	methionine metabolism	65.38	17 of 26
66794	tyrosine metabolism	64.29	9 of 14
66794	methylglyoxal degradation	60	3 of 5
66794	lysine metabolism	59.52	25 of 42
66794	non-pathway related	57.89	22 of 38
66794	heme metabolism	57.14	8 of 14
66794	propanol degradation	57.14	4 of 7
66794	tryptophan metabolism	55.26	21 of 38
66794	lipid metabolism	54.84	17 of 31
66794	pantothenate biosynthesis	50	3 of 6
66794	kanosamine biosynthesis II	50	1 of 2
66794	arginine metabolism	50	12 of 24
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	phenylmercury acetate degradation	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	ketogluconate metabolism	50	4 of 8
66794	proline metabolism	45.45	5 of 11
66794	vitamin B6 metabolism	45.45	5 of 11
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	glutathione metabolism	42.86	6 of 14
66794	mevalonate metabolism	42.86	3 of 7
66794	isoprenoid biosynthesis	42.31	11 of 26
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	carotenoid biosynthesis	40.91	9 of 22
66794	O-antigen biosynthesis	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	lipoate biosynthesis	40	2 of 5
66794	Entner Doudoroff pathway	40	4 of 10
66794	glycine metabolism	40	4 of 10
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	oxidative phosphorylation	39.56	36 of 91
66794	vitamin B1 metabolism	38.46	5 of 13
66794	urea cycle	38.46	5 of 13
66794	degradation of sugar alcohols	37.5	6 of 16
66794	ascorbate metabolism	36.36	8 of 22
66794	metabolism of disaccharids	36.36	4 of 11
66794	d-xylose degradation	36.36	4 of 11
66794	pentose phosphate pathway	36.36	4 of 11
66794	cyanate degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	degradation of hexoses	27.78	5 of 18
66794	arachidonic acid metabolism	27.78	5 of 18
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	cholesterol biosynthesis	27.27	3 of 11
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	biotin biosynthesis	25	1 of 4
66794	degradation of pentoses	25	7 of 28
66794	androgen and estrogen metabolism	25	4 of 16
66794	lactate fermentation	25	1 of 4
66794	sulfate reduction	23.08	3 of 13
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	molybdenum cofactor biosynthesis	22.22	2 of 9
66794	polyamine pathway	21.74	5 of 23

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121351	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Wastewater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6138	untreated municipal wastewater	Oklahoma, Norman	USA	USA	North America
57139	Untreated wastewater,water treatment plant	OK,Norman	USA	USA	North America
121351	Environment, Wastewater, untreated, treatment plant		United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AJ575430 (>99% sequence identity) for Cloacibacterium normanense from Microbeatlas

Aquatic Samples	4936
Soil Samples	350
Animal Samples	5409
Plant Samples	248
Total Samples	10943

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6138	1	Risk group (German classification) According to TRBA 466
121351	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM386056v1 assembly for Cloacibacterium normanense NRS-1	complete	GCA_003860565	237258.26	2848353471	237258	98.78
66792	IMG-taxon 2634166320 annotated assembly for Cloacibacterium normanense DSM 15886	scaffold	GCA_900104195	237258.25	2634166320	237258	70.88
124043	ASM174749v1 assembly for Cloacibacterium normanense NRS-1	contig	GCA_001747495	237258.24		237258	63.48

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6138	Cloacibacterium normanense partial 16S rRNA gene, type strain CCUG 46293	AJ575430	1475		237258

GC content

6138

GC-content (mol%)31

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM386056v1 assembly for Cloacibacterium normanense NRS-1
NCBI: GCA_003860565

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.66	no
125439	gram_stain	BacteriaNetⓘ	negative	99.66	no
125439	motility	BacteriaNetⓘ	no	80.65	no
125439	spore_formation	BacteriaNetⓘ	no	99.82	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Cloacibacterium normanense DSM 15886
NCBI: GCA_900104195.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.48	yes
125438	anaerobic	anaerobicⓘ	no	95.76	yes
125438	aerobic	aerobicⓘ	yes	78.73	no
125438	spore-forming	spore-formingⓘ	no	91.80	yes
125438	thermophilic	thermophileⓘ	no	98.07	yes
125438	flagellated	motile2+ⓘ	no	92.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	A primary assessment of the endophytic bacterial community in a xerophilous moss (Grimmia montana) using molecular method and cultivated isolates.	Liu XL, Liu SL, Liu M, Kong BH, Liu L, Li YH.	Braz J Microbiol	10.1590/s1517-83822014000100022	2014
	Draft Genome Sequence of Cloacibacterium normanense NRS-1 Isolated from Municipal Wastewater.	Gay NR, Fleming E, Oh J.	Genome Announc	10.1128/genomea.01397-16	2016
Phylogeny	Cloacibacterium caeni sp. nov., isolated from activated sludge.	Chun BH, Lee Y, Jin HM, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijsem.0.001841	2017
Phylogeny	Cloacibacterium rupense sp. nov., isolated from freshwater lake sediment.	Cao SJ, Deng CP, Li BZ, Dong XQ, Yuan HL	Int J Syst Evol Microbiol	10.1099/ijs.0.017681-0	2009
Phylogeny	Cloacibacterium normanense gen. nov., sp. nov., a novel bacterium in the family Flavobacteriaceae isolated from municipal wastewater.	Allen TD, Lawson PA, Collins MD, Falsen E, Tanner RS	Int J Syst Evol Microbiol	10.1099/ijs.0.64218-0	2006

References

#6138	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15886
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28036	IJSEM 1311 2006 ( DOI 10.1099/ijs.0.64218-0 , PubMed 16738108 )
#31763	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28036
#40491	; Curators of the CIP;
#57139	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 46293
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121351	Collection of Institut Pasteur ; Curators of the CIP; CIP 108613
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Cloacibacterium normanense (DSM 15886, ATCC BAA-825, CCUG 46293)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003860565

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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