Name: Alkalibacillus silvisoli BM2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 547

Type strain:

Species: Alkalibacillus silvisoli

Strain Designation: BM2

Culture col. no.: DSM 18495, JCM 14193, CIP 109569

Strain history: CIP <- 2007 JCM <- 2006, A. Echigo, Tokyo Univ., Saitama, Japan: strain BM2

NCBI tax ID(s): 392823 (species)

SI-ID 302749

DSM 18495, JCM 14193, CIP 109569

Section

Alkalibacillus silvisoli BM2 is an aerobe, spore-forming, mesophilic bacterium that was isolated from forest soil.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Alkalibacillus*
	Species	**Alkalibacillus silvisoli**
	Full Scientific Name (LPSN)	Alkalibacillus silvisoli Usami et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31957]	Gram stain	positive
[Ref.: #122612]	Gram stain	positive

[Ref.: #31957]	Cell length	04-07 µm

[Ref.: #31957]	Cell width	0.3-0.5 µm

[Ref.: #31957]	Cell shape	rod-shaped
[Ref.: #122612]	Cell shape	rod-shaped

[Ref.: #31957]	Motility	yes
[Ref.: #122612]	Motility	yes

[Ref.: #31957]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7563]

Culture medium

HALOBACTERIA MEDIUM (DSMZ Medium 372)

[Ref.: #7563]

Culture medium growth

[Ref.: #7563]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7563]

Culture medium composition

expand / minimize

[Ref.: #40069]

Culture medium

MEDIUM674 - for Alkalibacillus filiformis

[Ref.: #40069]

Culture medium growth

[Ref.: #122612]

Culture medium

CIP Medium 413

[Ref.: #122612]

Culture medium growth

[Ref.: #122612]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7563]	growth	30 °C
[Ref.: #31957]	growth	20-50 °C
[Ref.: #31957]	optimum	30-37 °C
[Ref.: #40069]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #7563]	Temperature range	mesophilic
[Ref.: #31957]	Temperature range	mesophilic
[Ref.: #40069]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31957]			optimum	9-9.5
[Ref.: #31957]			growth	07-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31957]

Oxygen tolerance

aerobe

[Ref.: #31957]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31957]		NaCl		growth	05-25	%
[Ref.: #31957]		NaCl		optimum	10-15	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31957]	galactose ChEBI	+	carbon source
[Ref.: #31957]	maltose ChEBI	+	carbon source
[Ref.: #31957]	mannitol ChEBI	+	carbon source
[Ref.: #122612]	nitrate ChEBI	-	reduction
[Ref.: #122612]	nitrite ChEBI	-	reduction
[Ref.: #31957]	sucrose ChEBI	+	carbon source
[Ref.: #31957]	trehalose ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #122612]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31957]	catalase	+	1.11.1.6
[Ref.: #122612]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #122612]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122612]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122612]	11963	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7563]	Sample type/isolated from	forest soil
[Ref.: #7563]	Geographic location (country and/or sea, region)	Saitama prefecture, Kawagoe City
[Ref.: #7563]	Country	Japan
[Ref.: #7563]	Country ISO 3 Code	JPN
[Ref.: #7563]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Non-saline surface soil from a forest in Kawagoe
[Ref.: #67770]	Geographic location (country and/or sea, region)	Saitama Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #122612]	Sample type/isolated from	Environment, Soil, non-saline forest
[Ref.: #122612]	Country	Japan
[Ref.: #122612]	Country ISO 3 Code	JPN
[Ref.: #122612]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB264528 (>99% sequence identity) for Alkalibacillus silvisoli from Microbeatlas

Aquatic Samples	7
Soil Samples	5
Animal Samples	7
Plant Samples	1
Total Samples	20

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7563]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122612]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31957]	Alkalibacillus silvisoli gene for 16S rRNA, partial sequence, strain: BM2	AB264528	1492	GenBank	392823 tax ID

[Ref.: #7563]	GC-content	37.0 mol%
[Ref.: #31957]	GC-content	37 mol%
[Ref.: #67770]	GC-content	37 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #7563]

Culture collection no.

DSM 18495, JCM 14193, CIP 109569

[Ref.: #70225]

SI-ID 302749

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Alkalibacillus silvisoli sp. nov., an alkaliphilic moderate halophile isolated from non-saline forest soil in Japan.	Usami R, Echigo A, Fukushima T, Mizuki T, Yoshida Y, Kamekura M	Int J Syst Evol Microbiol	10.1099/ijs.0.64713-0	2007	*

References

#7563

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18495

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31957

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28213 (see below)

#40069 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#70225

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID302749.1 )

#122612 Collection of Institut Pasteur ; Curators of the CIP; CIP 109569

#28213

IJSEM 770 2007 ( DOI 10.1099/ijs.0.64713-0 , PubMed 17392204 )

* These data were automatically processed and therefore are not curated

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