Eubacterium limosum (DSM 2593, JCM 10283, CCUG 16794)

Name: Eubacterium limosum (DSM 2593, JCM 10283, CCUG 16794)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5430

Type strain

Strain Designation 2A

Culture col. no. DSM 2593 JCM 10283 CCUG 16794 2A

NCBI tax ID(s) 1736

Special Collections DSMZ CCUG JCM

Links

Eubacterium limosum 2A is an anaerobe, mesophilic prokaryote that was isolated from river mud.

anaerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 47635

CCUG 16794,DSM 2593,JCM 10283

@ref 20215

Last LPSN update 2025-12-16 09:48:04

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Eubacteriaceae

Genus Eubacterium

Species Eubacterium limosum

Full scientific name Eubacterium limosum (Eggerth 1935) Prévot 1938 (Approved Lists 1980)

Synonyms (2)

"Butyribacterium limosum"
"Bacteroides limosus"

BacDive ID	Other strains from **Eubacterium limosum** (7)	Type strain
5432	E. limosum DSM 20543, ATCC 8486, NCIB 9763, CCUG 16793, ... (type strain)
5431	E. limosum llA, DSM 2594
141485	E. limosum CCUG 2228
143136	E. limosum CCUG 17367
153852	E. limosum CCUG 51363
157742	E. limosum G14, DSM 107592
165411	E. limosum JCM 6501, VPI 1939

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
1144	METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)	Medium recipe at MediaDive	Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a) Composition: Methanol 15.4688 g/l NaCl 2.19727 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NaHCO3 0.830078 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water
1144	CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)	Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water
1144	CHOPPED MEAT MEDIUM (DSMZ Medium 78)	Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1144	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

1144

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Aquatic	#River (Creek)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1144	river mud	Göttingen, Leine	Germany	DEU	Europe
46529	River mud	Göttingen,Leine	Germany	DEU	Europe
67770	River mud

Taxonmaps

69479

Global distribution of 16S sequence LC480790 (>99% sequence identity) for Eubacterium from Microbeatlas

Aquatic Samples	2430
Soil Samples	550
Animal Samples	38820
Plant Samples	312
Total Samples	42112

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1144	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Eubacterium limosum JCM 10283 gene for 16S ribosomal RNA, partial sequence	LC480790	1463		1736

GC content

1144

GC-content (mol%)46.4

Genome-based predictions

Literature

Title	Authors	Journal	DOI	Year
Refining and illuminating acetogenic Eubacterium strains for reclassification and metabolic engineering.	Flaiz M, Poehlein A, Wilhelm W, Mook A, Daniel R, Durre P, Bengelsdorf FR.	Microb Cell Fact	10.1186/s12934-024-02301-8	2024
Protocol to obtain genetically engineered Acetobacterium woodii and Eubacterium callanderi strains.	Baur KS, Ruhle B, Reith T, Krieg A, Bengelsdorf FR.	STAR Protoc	10.1016/j.xpro.2025.104011	2025
Clostridium butyricum-altered lung microbiome is associated with enhanced anti-influenza effects via G-protein-coupled receptor120.	Hagihara M, Yamashita M, Ariyoshi T, Minemura A, Yoshida C, Higashi S, Oka K, Takahashi M, Ota A, Maenaka A, Iwasaki K, Hirai J, Shibata Y, Umemura T, Mori T, Kato H, Asai N, Mikamo H.	iScience	10.1016/j.isci.2025.113502	2025

References

#1144	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2593
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#46529	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 16794
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Eubacterium limosum (DSM 2593, JCM 10283, CCUG 16794)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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