Name: Anaerobutyricum soehngenii L2-7
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5429

Type strain:

Species: Anaerobutyricum soehngenii

Strain Designation: L2-7

Culture col. no.: DSM 17630, KCTC 15707

Strain history: <- Sudarshan A Shetty, Wageningen Univ. and Research, The Netherlands

NCBI tax ID(s): 105843 (species)

SI-ID 297645

DSM 17630

Other strains from Anaerobutyricum soehngenii

A. soehngenii BSM-383-APC-4H, DSM 109238

Section

Anaerobutyricum soehngenii L2-7 is an anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from Infant, faecal sample.

colony-forming
Gram-positive
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Lachnospiraceae*
	Genus	*Anaerobutyricum*
	Species	**Anaerobutyricum soehngenii**
	Full Scientific Name (LPSN)	Anaerobutyricum soehngenii Shetty et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66751]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #66751]	Cell length	0.7-4 µm

[Ref.: #66751]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #66751]	Motility	no
[Ref.: #67771]	Motility	no

[Ref.: #66751]	Colony size	4.5 mm
[Ref.: #66751]	Colony color	white centred colonies surrounded by a colourless layer
[Ref.: #66751]	Colony shape	circular
[Ref.: #66751]	Incubation period	2 days
[Ref.: #66751]	Cultivation medium used	Reinforced Clostridial Medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7094]

Culture medium

RUMEN BACTERIA MEDIUM (DSMZ Medium 330)

[Ref.: #7094]

Culture medium growth

[Ref.: #7094]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7094]

Culture medium composition

expand / minimize

[Ref.: #7094]

Culture medium

SCHAEDLER BROTH (ROTH; 5772) (DSMZ Medium 1669)

[Ref.: #7094]

Culture medium growth

[Ref.: #7094]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7094]

Culture medium composition

expand / minimize

[Ref.: #66751]

Culture medium

Reinforced Clostridial Medium

[Ref.: #66751]

Culture medium growth

[Ref.: #66751]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7094]	growth	37 °C
[Ref.: #66751]	optimum	37 °C
[Ref.: #66751]	growth	35-45 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #7094]	Temperature range	mesophilic
[Ref.: #66751]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66751]			optimum	6.5
[Ref.: #66751]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #7094]	Oxygen tolerance	anaerobe
[Ref.: #66751]	Oxygen tolerance	obligate anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

[Ref.: #66751]

Observation

the major end-products of sugar fermentation are butyrate, formate, H2 and CO2. When grown on 1,2-propanediol, propionate, propanal and 1-propanol are produced. It requires acetate for better growth on substrates such as D,L-lactate and produces butyrate as major end product and small amounts of formate.

[Ref.: #66751]	Name of tolerated compound	Oxgall
[Ref.: #66751]	Tolerance percentage	0.1

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66751]	1,2-propandiol ChEBI	+	fermentation
[Ref.: #66751]	arabinose ChEBI	-	fermentation
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #66751]	cellobiose ChEBI	-	fermentation
[Ref.: #66751]	cellobiose ChEBI	-	growth
[Ref.: #66751]	D-fructose ChEBI	+	builds acid from
[Ref.: #66751]	D-glucose ChEBI	+	builds acid from
[Ref.: #66751]	D-mannitol ChEBI	-	growth
[Ref.: #66751]	D-mannose ChEBI	+	builds acid from
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #66751]	D-rhamnose ChEBI	-	growth
[Ref.: #66751]	D-xylose ChEBI	-	growth
[Ref.: #66751]	esculin ChEBI	-	hydrolysis
[Ref.: #66751]	fructose ChEBI	+	fermentation
[Ref.: #66751]	galactose ChEBI	+	builds acid from
[Ref.: #66751]	galactose ChEBI	+	fermentation
[Ref.: #66751]	glucose ChEBI	+	fermentation
[Ref.: #66751]	L-arabinose ChEBI	-	growth
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #66751]	lactose ChEBI	-	fermentation
[Ref.: #66751]	lactose ChEBI	-	growth
[Ref.: #66751]	maltose ChEBI	+	builds acid from
[Ref.: #66751]	maltose ChEBI	+	fermentation
[Ref.: #66751]	mannitol ChEBI	-	fermentation
[Ref.: #66751]	mannose ChEBI	+	fermentation
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #66751]	rhamnose ChEBI	-	fermentation
[Ref.: #66751]	ribose ChEBI	-	fermentation
[Ref.: #66751]	sorbitol ChEBI	+	builds acid from
[Ref.: #66751]	sorbitol ChEBI	+	fermentation
[Ref.: #66751]	sucrose ChEBI	+	builds acid from
[Ref.: #66751]	sucrose ChEBI	+	fermentation
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #66751]	xylose ChEBI	-	fermentation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66751]		indole ChEBI	no
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #66751]	catalase	-	1.11.1.6
[Ref.: #66751]	cytochrome oxidase	-	1.9.3.1
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #7094]	12657	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #7094]	12656	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7094]	Sample type/isolated from	Infant, faecal sample
[Ref.: #7094]	Geographic location (country and/or sea, region)	United Kingdom
[Ref.: #7094]	Continent	Europe

[Ref.: #66751]	Sample type/isolated from	human infant faeces

[Ref.: #67771]	Sample type/isolated from	From Infant, faecal sample
[Ref.: #67771]	Country	United Kingdom
[Ref.: #67771]	Country ISO 3 Code	GBR
[Ref.: #67771]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Child

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ270490 (>99% sequence identity) for Lachnospiraceae from Microbeatlas

Aquatic Samples
Soil Samples
Animal Samples	126
Plant Samples
Total Samples	126

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7094]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66751]	Butyrate-producing bacterium L2-7 16S rRNA gene	AJ270490	1482	ENA	105843 tax ID

[Ref.: #66751]	GC-content	38.6 mol%	genome sequence analysis
[Ref.: #67771]	GC-content	38.6 mol%

Availability in culture collections External links

[Ref.: #7094]

Culture collection no.

DSM 17630, KCTC 15707

[Ref.: #74917]

SI-ID 297645

Literature:

Topic	Title	Authors	Journal	DOI	Year
Transcriptome	Duodenal Anaerobutyricum soehngenii infusion stimulates GLP-1 production, ameliorates glycaemic control and beneficially shapes the duodenal transcriptome in metabolic syndrome subjects: a randomised double-blind placebo-controlled cross-over study.	Koopen A, Witjes J, Wortelboer K, Majait S, Prodan A, Levin E, Herrema H, Winkelmeijer M, Aalvink S, Bergman JJGHM, Havik S, Hartmann B, Levels H, Bergh PO, van Son J, Balvers M, Bastos DM, Stroes E, Groen AK, Henricsson M, Kemper EM, Holst J, Strauch CM, Hazen SL, Backhed F, De Vos WM, Nieuwdorp M, Rampanelli E	Gut	10.1136/gutjnl-2020-323297	2021	*
Phylogeny	Reclassification of Eubacterium hallii as Anaerobutyricum hallii gen. nov., comb. nov., and description of Anaerobutyricum soehngenii sp. nov., a butyrate and propionate-producing bacterium from infant faeces.	Shetty SA, Zuffa S, Bui TPN, Aalvink S, Smidt H, De Vos WM	Int J Syst Evol Microbiol	10.1099/ijsem.0.003041	2018	*
Enzymology	Distribution, organization and expression of genes concerned with anaerobic lactate utilization in human intestinal bacteria.	Sheridan PO, Louis P, Tsompanidou E, Shaw S, Harmsen HJ, Duncan SH, Flint HJ, Walker AW	Microb Genom	10.1099/mgen.0.000739	2022	*

References

#7094

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17630

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66751

Sudarshan A. Shetty, Simone Zuffa, Thi Phuong Nam Bui, Steven Aalvink, Hauke Smidt, Willem M. De Vos: Reclassification of Eubacterium hallii as Anaerobutyricum hallii gen. nov., comb. nov., and description of Anaerobutyricum soehngenii sp. nov., a butyrate and propionate-producing bacterium from infant faeces. IJSEM 68: 3741 - 3746 2018 ( DOI 10.1099/ijsem.0.003041 , PubMed 30351260 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#74917

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID297645.1 )

* These data were automatically processed and therefore are not curated

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