Name: Enterococcus lactis BT159
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5362

Type strain:

Species: Enterococcus lactis

Strain Designation: BT159

Culture col. no.: DSM 23655, LMG 25958

Strain history: <- S. Morandi, National Research Council - Institute of Science of Food Production; BT 159

NCBI tax ID(s): 357441 (species)

SI-ID 370344

LMG 25958, DSM 23655, CCM 8412

Other strains from Enterococcus lactis

E. lactis JCM 30200, CCTCC AB 2014041

Section

Enterococcus lactis BT159 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from raw milk cheese .

coccus-shaped
Gram-positive
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus lactis**
	Full Scientific Name (LPSN)	Enterococcus lactis Morandi et al. 2012

Synonym

Enterococcus xinjiangensis

Information on morphological and physiological properties Morphology

[Ref.: #30210]	Gram stain	positive

[Ref.: #30210]	Cell shape	coccus-shaped

[Ref.: #30210]	Motility	no

[Ref.: #17327]	Type of hemolysis	gamma
[Ref.: #17327]	Incubation period	1-2 days

[Ref.: #30210]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17327]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #17327]

Culture medium growth

[Ref.: #17327]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17327]

Culture medium composition

expand / minimize

[Ref.: #17327]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #17327]

Culture medium growth

[Ref.: #17327]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17327]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #17327]			growth	37 °C
[Ref.: #30210]			growth	10-45 °C

[Ref.: #17327]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #30210]			growth	9.6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #17327]	Oxygen tolerance	microaerophile
[Ref.: #30210]	Oxygen tolerance	facultative anaerobe

[Ref.: #30210]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30210]		NaCl		growth	2-6.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30210]	arabinose ChEBI	+	carbon source
[Ref.: #68370]	arginine ChEBI	+	hydrolysis	* from API 20STR, API rID32STR
[Ref.: #30210]	cellobiose ChEBI	+	carbon source
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68370]	D-mannitol ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68370]	D-ribose ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68370]	D-sorbitol ChEBI	-	builds acid from	* from API 20STR
[Ref.: #68381]	D-tagatose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30210]	esculin ChEBI	+	hydrolysis
[Ref.: #68370]	esculin ChEBI	+	hydrolysis	* from API 20STR
[Ref.: #30210]	fructose ChEBI	+	carbon source
[Ref.: #30210]	galactose ChEBI	+	carbon source
[Ref.: #30210]	glucose ChEBI	+	carbon source
[Ref.: #68370]	glycogen ChEBI	-	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68370]	hippurate ChEBI	+	hydrolysis	* from API 20STR, API rID32STR
[Ref.: #68370]	inulin ChEBI	-	builds acid from	* from API 20STR
[Ref.: #68370]	L-arabinose ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #30210]	lactose ChEBI	+	carbon source
[Ref.: #68370]	lactose ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #30210]	maltose ChEBI	+	carbon source
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30210]	mannitol ChEBI	+	carbon source
[Ref.: #30210]	mannose ChEBI	+	carbon source
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #30210]	melibiose ChEBI	+	carbon source
[Ref.: #68381]	melibiose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30210]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68370]	raffinose ChEBI	-	builds acid from	* from API 20STR, API rID32STR
[Ref.: #30210]	ribose ChEBI	+	carbon source
[Ref.: #30210]	salicin ChEBI	+	carbon source
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68370]	starch ChEBI	+	builds acid from	* from API 20STR
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30210]	trehalose ChEBI	+	carbon source
[Ref.: #68370]	trehalose ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68370]		acetoin ChEBI	yes	* from API 20STR, API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR, API 20STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR, API 20STR
[Ref.: #68381]	alpha-galactosidase	-	3.2.1.22	* from API rID32STR, API 20STR
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR, API 20STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR, API 20STR
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR, API 20STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR, API 20STR
[Ref.: #68381]	beta-mannosidase	+	3.2.1.25	* from API rID32STR
[Ref.: #17327]	catalase	-	1.11.1.6
[Ref.: #17327]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68370]	leucine arylamidase	+	3.4.11.1	* from API 20STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32STR
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR, API 20STR
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® 20STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Acetoin production (Voges Proskauer test)VP	Hippuric acid hydrolysisHIP	Esculin hydrolysis/beta-glucosidaseESC	Pyrrolidonyl arylamidasePYRA	alpha-Galactosidasealpha GAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	Alkaline phosphatasePAL	Leucine arylamidaseLAP	Arginine dihydrolaseADH	Acid from D-riboseRIB	Acid from L-arabinoseARA	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	beta hemolysis presentbeta HEM
[Ref.: #17327]	843	+	+	+	+	-	-	+	-	+	+	+	+	+	-	+	+	-	-	+	-	-

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #17327]	10562	+	+	+	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	+	-	-	+	-	-	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17327]	Sample type/isolated from	raw milk cheese (Bitto PDO)
[Ref.: #17327]	Geographic location (country and/or sea, region)	Valtellina
[Ref.: #17327]	Country	Italy
[Ref.: #17327]	Country ISO 3 Code	ITA
[Ref.: #17327]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Dairy product

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GU983697 (>99% sequence identity) for Enterococcus from Microbeatlas

Aquatic Samples	2532
Soil Samples	1007
Animal Samples	51351
Plant Samples	654
Total Samples	55544

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17327]	Enterococcus lactis strain BT159 16S ribosomal RNA gene, partial sequence	GU983697	1437	ENA	357441 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus lactis LMG 25958	GCA_015904215	scaffold	GenBank	357441 tax ID	*
[Ref.: #66792]	Enterococcus lactis strain LMG 25958	357441.21	wgs	PATRIC	357441 tax ID	*
[Ref.: #66792]	Enterococcus lactis DSM 23655	2928549275	draft	JGI IMG/M	357441 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus lactis LMG 25958 NCBI: GCA_015904215.1
[Ref.: #69480]	flagellated	no	97.846	yes
[Ref.: #69480]	gram-positive	yes	94.833	no
[Ref.: #69480]	anaerobic	no	97.422	no
[Ref.: #69480]	aerobic	no	93.624	no
[Ref.: #69480]	halophile	yes	91.581	no
[Ref.: #69480]	spore-forming	no	91.209	no
[Ref.: #69480]	thermophile	no	99.738	yes
[Ref.: #69480]	glucose-util	yes	89.058	no
[Ref.: #69480]	motile	no	93.94	no
[Ref.: #69480]	glucose-ferment	yes	86.776	no

Availability in culture collections External links

[Ref.: #17327]

Culture collection no.

DSM 23655, LMG 25958

[Ref.: #74852]

SI-ID 370344

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Enterococcus lactis sp. nov., from Italian raw milk cheeses.	Morandi S, Cremonesi P, Povolo M, Brasca M	Int J Syst Evol Microbiol	10.1099/ijs.0.030825-0	2011	*

References

#17327

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23655

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30210

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26555 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68370

Automatically annotated from API 20STR .

#68381

Automatically annotated from API rID32STR .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#74852

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID370344.1 )

#26555

IJSEM 1992 2012 ( DOI 10.1099/ijs.0.030825-0 , PubMed 22003038 )

* These data were automatically processed and therefore are not curated

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Enterococcus lactis LMG 25958

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