Name: Enterococcus hirae M 19
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5315

Type strain:

Species: Enterococcus hirae

Strain Designation: M 19

Culture col. no.: CCUG 32258, NCIMB 8192, CCM 4533, DSM 3320, ATCC 10541, CIP 58.55, NCIB 8192, WDCM 00011, NBRC 113010, LMG 10274, CECT 4081, NCTC 13383

Strain history: CIP <- 1958, NCIB <- 1950, ATCC <- FDA, Washington, USA: strain M19, Streptococcus faecalis

NCBI tax ID(s): 1354 (species)

SI-ID 2257

LMG 10274, ATCC 10541, NCIMB 8192, NCIB 8192, CCUG 32258, CCM 4533, CIP 58.55, CECT 4081, CCRC 11547, CECT 214, KCTC 2012, BCRC 11547, KACC 10779, NCAIM B.02062, CDBB 1087, ACM 5050, CNCTC 5651, CGMCC 1.0997, DSM 3320

Other strains from Enterococcus hirae

E. hirae DSM 20160, ATCC 8043, CCM 2423, ... (type strain)
E. hirae SB, DSM 28619
E. hirae Eh61, DSM 27815
E. hirae AmMS 206, CIP 103564, ATCC 49135
E. hirae CCUG 19908 B
E. hirae CCUG 28689
E. hirae CCUG 28690
E. hirae CCUG 33114
E. hirae CCUG 36850
E. hirae CCUG 37829
E. hirae CCUG 42698
E. hirae CCUG 46536
E. hirae CCUG 47453
E. hirae CCUG 51711
E. hirae CCUG 53265
E. hirae CCUG 54371
E. hirae CCUG 58425
E. hirae WCA-386-APC-3D, DSM 100949
E. hirae Cla-CZ-137, DSM 109134
E. hirae JCM 8717
E. hirae JCM 8718
E. hirae JCM 8719
E. hirae JCM 8720

Section

Enterococcus hirae M 19 is a microaerophile, spore-forming, mesophilic human pathogen that has multiple antibiotic resistances.

antibiotic resistance
spore-forming
human pathogen
mesophilic
microaerophile
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus hirae**
	Full Scientific Name (LPSN)	Enterococcus hirae Farrow and Collins 1985

Information on morphological and physiological properties Morphology

[Ref.: #119907]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #119907]	Cell shape	coccus-shaped

[Ref.: #119907]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %90.636

[Ref.: #1357]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1357]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #1357]

Culture medium growth

[Ref.: #1357]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1357]

Culture medium composition

expand / minimize

[Ref.: #1357]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #1357]

Culture medium growth

[Ref.: #1357]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1357]

Culture medium composition

expand / minimize

[Ref.: #41826]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #41826]

Culture medium growth

[Ref.: #41826]

Culture medium composition

expand / minimize

[Ref.: #119907]

Culture medium

CIP Medium 29

[Ref.: #119907]

Culture medium growth

[Ref.: #119907]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #1357]	growth	37 °C
[Ref.: #41826]	growth	30 °C
[Ref.: #119907]	growth	10-45 °C

[Ref.: #1357]	Temperature range	mesophilic
[Ref.: #41826]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #1357]	Oxygen tolerance	microaerophile
[Ref.: #119907]	Oxygen tolerance	facultative anaerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Test 5

Test 6

Test 7

Test 8

Test 9

Reference

[Ref.: #1357]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
22-24
12
26-28
26-28
20-22
16-18
10
14
resistant0
26
30
26-28
16
14
20-22
26-28
10
22
22
resistant0
resistant0
24
20
resistant0
16-18
16-18
28-30
14
10
20
24
22-24
resistant0
20
20
20

[Ref.: #1357]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
22-24
10
26-28
28
20
18
10
12
resistant0
26
30-32
28
12
12
20
28-30
8
20
22
resistant0
resistant0
18
20
resistant0
12
12
30
16
12
20
24
28
resistant0
22
20
20

[Ref.: #1357]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
24
10
30
26-28
22
18
10-12
16
resistant0
26
30
26-28
12
12
22
28
8-10
22
24
resistant0
resistant0
18
22
resistant0
12
14
30
18
10
20
24
28-30
resistant0
20-22
20
20

[Ref.: #1357]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
18
resistant0
18
18-20
16-18
8
resistant0
resistant0
resistant0
18-20
18-20
16-18
8
6
14
14
6
12
resistant0
resistant0
resistant0
10
14
resistant0
8
6
18-20
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #1357]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
22
resistant0
24
24-26
20
14
8
resistant0
resistant0
24
30-32
26
14
12
22
28
8
22
24
resistant0
resistant0
20
22
resistant0
24
14
32-34
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #1357]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
20-22
8
26
24
20
14
12
resistant0
resistant0
24
32
24
12
12
20
26
8
20
22
resistant0
resistant0
16-18
18
resistant0
12
12
30
resistant0
10
20
24
28
resistant0
20
18
20

[Ref.: #1357]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
18
resistant0
18
18-20
16-18
8
resistant0
resistant0
resistant0
18-20
18-20
16-18
8
6
14
14
6
12
resistant0
resistant0
resistant0
10
14
resistant0
8
6
18-20
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #1357]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
20-22
8
26
24
20
14
12
resistant0
resistant0
24
32
24
12
12
20
26
8
20
22
resistant0
resistant0
16-18
18
resistant0
12
12
30
resistant0
10
20
24
28
resistant0
20
18
20

[Ref.: #1357]
Mueller Hinton
37

microaerophil
yes
Inhibition zone diameter in mm
22
10
26
24
20
14-16
10
10
resistant0
24-26
28
24
12
12
20
28
6
20-22
22
resistant0
resistant0
18
18
resistant0
12
12
30
14
10
20
24
28
resistant0
20
20
20

Only first 15 entries are displayed. Click here to see all.

[Ref.: #69481]	Ability of spore formation	yes Confidence in %97
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.889

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #119907]		NaCl		growth	6.5	%

[Ref.: #1357]	Murein short key	A11.31
[Ref.: #1357]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-ribose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	L-arabinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #119907]	nitrate ChEBI	-	reduction
[Ref.: #119907]	nitrite ChEBI	-	reduction
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #119907]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #119907]		indole ChEBI	no
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #119907]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	+	3.2.1.22	* from API rID32STR
[Ref.: #119907]	amylase	-
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #1357]	catalase	-	1.11.1.6
[Ref.: #119907]	catalase	-	1.11.1.6
[Ref.: #1357]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #119907]	gelatinase	-
[Ref.: #119907]	lysine decarboxylase	-	4.1.1.18
[Ref.: #119907]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR
[Ref.: #119907]	urease	-	3.5.1.5
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #1357]	10840	+	+	+	-	+	-	+	+	-	-	+/-	-	+	-	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	+	+	-
[Ref.: #1357]	10786	+	+	+	-	+	-	+	-	-	-	+	-	+	-	-	+	+	+	+	+	+	+	+	-	-	+	+/-	-	+	+	+	-
[Ref.: #1357]	10723	+	+	+	-	+	-	+	-	-	-	+	-	+	-	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	-	+/-	-
[Ref.: #50934]	3408	+	+	-	-	+	-	+	-	-	+	+	-	+	-	-	+	+	-	+	+	-	+	-	-	-	+	-	-	+	+	+	-
[Ref.: #1357]	10195	+	+	+	-	+	-	+	-	-	+	+	-	+	-	-	+	+	+/-	+	+	+	+	-	-	-	+	+	-	+	+	+	-
[Ref.: #1357]	10194	+	+	-	-	+	-	+	-	-	-	+	-	+	-	-	+	+	-	+	+	+/-	+/-	-	-	-	+	+	-	+	+	+	-
[Ref.: #1357]	10193	+	+	+	-	+	-	+	-	-	-	+	-	+	-	-	+	+	+	+	+	+	+	-	-	-	+	+/-	-	+	-	+/-	-
[Ref.: #1357]	10192	+	+	+	-	+	-	+	-	-	-	+	-	+	-	-	+	+	-	+	+	+	+	-	-	-	+	+	-	+	+	+	-
[Ref.: #1357]	10885	+	+	-	-	+	-	+	-	-	-	+	-	+	-	-	+	+	-	+	+	+	+	-	-	-	+	+/-	-	+	+	+	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1357]	Pathogenicity (human)	yes
[Ref.: #1357]	Pathogenicity (animal)	yes
[Ref.: #1357]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119907]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus hirae strain FDAARGOS_1057	1354.358	complete	PATRIC	1354 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Enterococcus hirae strain FDAARGOS_1057 strain Not applicable PATRIC: 1354.358
[Ref.: #69481]	spore-forming	yes	97	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus hirae strain FDAARGOS_1057 strain Not applicable PATRIC: 1354.358
[Ref.: #69480]	motile	no	96.165	no
[Ref.: #69480]	gram-positive	yes	93.887	no
[Ref.: #69480]	anaerobic	no	96.334	yes
[Ref.: #69480]	halophile	yes	91.726	no
[Ref.: #69480]	spore-forming	no	93.014	no
[Ref.: #69480]	thermophile	no	99.738	yes
[Ref.: #69480]	glucose-util	yes	88.425	no
[Ref.: #69480]	flagellated	no	98.216	no
[Ref.: #69480]	aerobic	no	95.937	yes
[Ref.: #69480]	glucose-ferment	yes	89.867	no

Availability in culture collections External links

[Ref.: #1357]

Culture collection no.

CCUG 32258, NCIMB 8192, CCM 4533, DSM 3320, ATCC 10541, CIP 58.55, NCIB 8192, WDCM 00011, NBRC 113010, LMG 10274, CECT 4081, NCTC 13383

[Ref.: #74804]

SI-ID 2257

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Enterococcus hirae, Enterococcus faecium and Enterococcus faecalis show different sensitivities to typical biocidal agents used for disinfection.	Suchomel M, Lenhardt A, Kampf G, Grisold A	J Hosp Infect	10.1016/j.jhin.2019.08.014	2019	*
Enzymology	The application of the loop-mediated isothermal amplification (LAMP) method for diagnosing Enterococcus hirae-associated endocarditis outbreaks in chickens.	Dolka B, Cisek AA, Szeleszczuk P	BMC Microbiol	10.1186/s12866-019-1420-z	2019	*
Pathogenicity	Isopropanol at 60% and at 70% are effective against 'isopropanol-tolerant' Enterococcus faecium.	Gebel J, Gemein S, Kampf G, Pidot SJ, Buetti N, Exner M	J Hosp Infect	10.1016/j.jhin.2019.01.024	2019	*
Pathogenicity	Neutralization efficiency of alcohol based products used for rapid hand disinfection	Chojecka A, Tarka P, Kierzkowska A, Nitsch-Osuch A, Kanecki K	Rocz Panstw Zakl Hig		2017	*
Pathogenicity	Enterococcus hirae biofilm formation on hospital material surfaces and effect of new biocides.	Di Lodovico S, Cataldi V, Di Campli E, Ancarani E, Cellini L, Di Giulio M	Environ Health Prev Med	10.1186/s12199-017-0670-3	2017	*
Pathogenicity	Resistance of enterococci to heat and chemical agents.	Renner P, Peters J	Zentralbl Hyg Umweltmed		1999	*
Pathogenicity	[Experimental study of the antibacterial activity of cloth impregnated with a disinfectant solution].	Poinsot C, Blanc G, Garcin F, Joly B	Pathol Biol (Paris)		1993	*
	Antimicrobial efficacy of the combination of chlorhexidine digluconate and dexpanthenol.	Kramer A, Assadian O, Koburger-Janssen T	GMS Hyg Infect Control	10.3205/dgkh000284	2016	*
	Effect of the amino chain length and the transformation into citric acid salts of aryl-diphenyl-butenes and ferrocenyl-diphenyl-butenes bearing two dimethylaminoalkyl chains on their antimicrobial activities.	Jellali K, Pigeon P, Trigui F, Top S, Aifa S, Jaouen G, El Arbi M	Springerplus	10.1186/2193-1801-2-508	2013	*
	Component Composition and Biological Activity of Various Extracts of Onosma gmelinii (Boraginaceae).	Shilov SV, Ustenova GO, Kiyekbayeva LN, Korotetskiy IS, Kudashkina NV, Zubenko NV, Parenova RA, Jumagaziyeva AB, Iskakbayeva ZA, Kenesheva ST	Int J Biomater	10.1155/2022/4427804	2022	*

References

#1357

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3320

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41826 ; Curators of the CIP;
#50934 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32258

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68381

Automatically annotated from API rID32STR .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#74804

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID2257.1 )

#119907 Collection of Institut Pasteur ; Curators of the CIP; CIP 58.55
* These data were automatically processed and therefore are not curated

Change proposal

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Search for species Enterococcus hirae in external resources:
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Enterococcus hirae strain FDAARGOS_1057 strain Not applicable

Enterococcus hirae strain FDAARGOS_1057 strain Not applicable

Change proposal

Change proposal