Name: Enterococcus casseliflavus DSM 25781
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5277

Type strain:

Species: Enterococcus casseliflavus

Culture col. no.: DSM 25781, NCCP 11515

Strain history: <- National Culture Collection of Pathogens, South Korea; NCCP 11515 <- Korean National Institute of Health

NCBI tax ID(s): 37734 (species)

SI-ID 408126

DSM 25781

Other strains from Enterococcus casseliflavus

Section

Enterococcus casseliflavus DSM 25781 is a microaerophile, mesophilic, Gram-positive bacterium that has multiple antibiotic resistances and was isolated from human stool.

antibiotic resistance
Gram-positive
mesophilic
microaerophile
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus casseliflavus**
	Full Scientific Name (LPSN)	Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984

	Synonym	"Streptococcus* faecium subsp. casseliflavus"*
	Synonym	Enterococcus flavescens
	Synonym	"Streptococcus* casseliflavus"*

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #14778]	Type of hemolysis	alpha
[Ref.: #14778]	Hemolysis ability	1
[Ref.: #14778]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #14778]

Culture medium

UREAPLASMA MEDIUM (DSMZ Medium 1096)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #14778]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #14778]			growth	37 °C

[Ref.: #14778]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #14778]

Oxygen tolerance

microaerophile

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Reference

[Ref.: #14778]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
22-24
resistant0
28-30
24-26
26
20
18
resistant0
8
24
34-36
28
20
16
8
28-30
10
20
22-24
resistant0
8-10
26-28
14-16
12
16
18
34-36
resistant0
12
20
26
32-34
resistant0
20
10
22

[Ref.: #14778]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
20
resistant0
24-26
20
20
16-18
12-14
resistant0
resistant0
20
32-34
26
18
14-16
resistant0
22-24
10-12
16
18
resistant0
resistant0
24-26
14-16
10
12-14
16-18
34
resistant0
resistant0
18
24-26
30-32
resistant0
18
10
20

[Ref.: #14778]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
20
resistant0
24-26
20
20
16-18
12-14
resistant0
resistant0
20
32-34
26
18
14-16
resistant0
22-24
10-12
16
18
resistant0
resistant0
24-26
14-16
10
12-14
16-18
34
resistant0
resistant0
18
24-26
30-32
resistant0
18
10
20

Only first 15 entries are displayed. Click here to see all.

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.941

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	L-arabinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	lactose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melibiose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	+	3.2.1.22	* from API rID32STR
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	+	3.2.1.25	* from API rID32STR
[Ref.: #14778]	catalase	+	1.11.1.6
[Ref.: #14778]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32STR
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #14778]	10777	+	+	+	-	+	-	-	+	-	+	+	+	+	+	-	-	+	-	+	+	+	+	-	-	-	+	+	-	+	-	+	-
[Ref.: #14778]	10592	+	+	+	-	+	-	+/-	+	-	+	+	+	+	+	-	-	+	-	+	+	+	+	-	-	-	+	+	+	+	+/-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #14778]	Sample type/isolated from	human stool
[Ref.: #14778]	Geographic location (country and/or sea, region)	Seoul
[Ref.: #14778]	Country	Republic of Korea
[Ref.: #14778]	Country ISO 3 Code	KOR
[Ref.: #14778]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #14778]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus casseliflavus strain FDAARGOS_1122	37734.152	complete	PATRIC	37734 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Enterococcus casseliflavus strain FDAARGOS_1122 strain Not applicable PATRIC: 37734.152
[Ref.: #69481]	spore-forming	yes	70	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus casseliflavus strain FDAARGOS_1122 strain Not applicable PATRIC: 37734.152
[Ref.: #69480]	gram-positive	yes	89.612	no
[Ref.: #69480]	anaerobic	no	96.92	yes
[Ref.: #69480]	halophile	yes	70.289	no
[Ref.: #69480]	spore-forming	no	75.566	no
[Ref.: #69480]	glucose-util	yes	88.855	no
[Ref.: #69480]	aerobic	no	95.338	yes
[Ref.: #69480]	motile	yes	51.447	no
[Ref.: #69480]	flagellated	no	81.448	no
[Ref.: #69480]	thermophile	no	98.722	no
[Ref.: #69480]	glucose-ferment	yes	87.204	no

Availability in culture collections External links

[Ref.: #14778]

Culture collection no.

DSM 25781, NCCP 11515

[Ref.: #74767]

SI-ID 408126

References

#14778

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25781

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68381

Automatically annotated from API rID32STR .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#74767

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID408126.1 )

* These data were automatically processed and therefore are not curated

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Enterococcus casseliflavus strain FDAARGOS_1122 strain Not applicable

Enterococcus casseliflavus strain FDAARGOS_1122 strain Not applicable

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