Name: Enterococcus casseliflavus 32488
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5275

Type strain:

Species: Enterococcus casseliflavus

Strain Designation: 32488

Culture col. no.: CCUG 34231, DSM 20382

Strain history: <- Kiel, 32488 (Streptococcus faecium)

NCBI tax ID(s): 37734 (species)

SI-ID 49620

CCUG 34231, DSM 20382

Other strains from Enterococcus casseliflavus

Section

Enterococcus casseliflavus 32488 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from raw milk.

Gram-positive
mesophilic
microaerophile
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus casseliflavus**
	Full Scientific Name (LPSN)	Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984

	Synonym	"Streptococcus* faecium subsp. casseliflavus"*
	Synonym	Enterococcus flavescens
	Synonym	"Streptococcus* casseliflavus"*

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8634]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8634]

Culture medium growth

[Ref.: #8634]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8634]

Culture medium composition

expand / minimize

[Ref.: #8634]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #8634]

Culture medium growth

[Ref.: #8634]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8634]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8634]			growth	37 °C

[Ref.: #8634]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8634]

Oxygen tolerance

microaerophile

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.802

[Ref.: #8634]	Murein short key	A11.31
[Ref.: #8634]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32STR
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #8634]	10793	+	+	+	+	+	-	+	+	+	+	+	+	+	+	-	-	+	-	+	+	+	+	-	-	-	+	+	+	+	+	+	-
[Ref.: #8634]	10404	+	+	-	-	+/-	-	+	+	+/-	+	+	+	+	+	-	-	+	-	+	+	+	+	-	-	-	+	+	-	+	+	+	-
[Ref.: #8634]	10403	-	+	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	-	-	-	+/-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8634]	Sample type/isolated from	raw milk

[Ref.: #52000]	Sample type/isolated from	Raw milk
[Ref.: #52000]	Geographic location (country and/or sea, region)	Kiel ?
[Ref.: #52000]	Country	Germany
[Ref.: #52000]	Country ISO 3 Code	DEU
[Ref.: #52000]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host Body Product	#Fluids	#Milk
#Host	#Mammals	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8634]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus casseliflavus strain FDAARGOS_1121	37734.150	complete	PATRIC	37734 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Enterococcus casseliflavus strain FDAARGOS_1121 strain Not applicable PATRIC: 37734.150
[Ref.: #69481]	spore-forming	no	75	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus casseliflavus strain FDAARGOS_1121 strain Not applicable PATRIC: 37734.150
[Ref.: #69480]	gram-positive	yes	88.054	no
[Ref.: #69480]	anaerobic	no	97.258	yes
[Ref.: #69480]	halophile	yes	67.077	no
[Ref.: #69480]	spore-forming	no	67.116	no
[Ref.: #69480]	glucose-util	yes	85.918	no
[Ref.: #69480]	aerobic	no	95.127	yes
[Ref.: #69480]	motile	yes	54.348	no
[Ref.: #69480]	flagellated	no	77.522	no
[Ref.: #69480]	thermophile	no	99.711	no
[Ref.: #69480]	glucose-ferment	yes	85.56	no

Availability in culture collections External links

[Ref.: #8634]

Culture collection no.

CCUG 34231, DSM 20382

[Ref.: #74765]

SI-ID 49620

References

#8634

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20382

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#52000 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 34231

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68381

Automatically annotated from API rID32STR .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#74765

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49620.1 )

* These data were automatically processed and therefore are not curated

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Search for species Enterococcus casseliflavus in external resources:
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Enterococcus casseliflavus strain FDAARGOS_1121 strain Not applicable

Enterococcus casseliflavus strain FDAARGOS_1121 strain Not applicable

Change proposal

Change proposal