Thermocrinis minervae (DSM 19557, ATCC BAA-1533, CR11)

Name: Thermocrinis minervae (DSM 19557, ATCC BAA-1533, CR11)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 525

Type strain

Strain Designation CR11

Culture col. no. DSM 19557 ATCC BAA-1533 CR11

NCBI tax ID(s) 381751

Special Collections DSMZ Literature strains

History <- A.-L. Reysenbach, Dept. Biology, Portland State Univ., USA; CR11

Links

Thermocrinis minervae CR11 is a microaerophile, chemolithotroph, Gram-negative bacterium that was isolated from terrestrial hot spring.

Gram-negative motile rod-shaped microaerophile chemolithotroph genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 405654

ATCC BAA-1533,DSM 19557

@ref 20215

Last LPSN update 2026-02-09 11:17:53

Domain Bacteria

Phylum Aquificota

Class Aquificia

Order Aquificales

Family Aquificaceae

Genus Thermocrinis

Species Thermocrinis minervae

Full scientific name Thermocrinis minervae Caldwell et al. 2010

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Confidence
23130	negative	2.4-3.9 µm	0.5-0.6 µm	rod-shaped
125438	negative					98.955

Multimedia

@ref:	66793
multimedia content:	EM_DSM_19557_1.jpg
multimedia.multimedia content:	EM_DSM_19557_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_19557_2.jpg
multimedia.multimedia content:	EM_DSM_19557_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_19557_3.jpg
multimedia.multimedia content:	EM_DSM_19557_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_19557_4.jpg
multimedia.multimedia content:	EM_DSM_19557_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_19557_5.jpg
multimedia.multimedia content:	EM_DSM_19557_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8174	MS-MEDIUM (MODIFIED) (DSMZ Medium 1145)		Medium recipe at MediaDive	Name: MS-MEDIUM (MODIFIED) (DSMZ Medium 1145) Composition: MgSO4 x 7 H2O 7.0 g/l Elemental sulphur 5.0 g/l MgCl2 x 6 H2O 2.0 g/l NH4Cl 2.0 g/l NaS2O3 x 5 H2O 2.0 g/l CaCl2 x 2 H2O 0.8 g/l NaOH 0.79994 g/l KCl 0.48 g/l K2HPO4 x 3 H2O 0.3 g/l Na2-EDTA x 2 H2O 0.005 g/l CoCl2 x 6 H2O 0.0015 g/l ZnCl2 0.001 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l AlCl3 x 6 H2O 0.0004 g/l Na2WO4 x 2 H2O 0.0003 g/l CuCl2 x 2 H2O 0.0002 g/l NiSO4 x 6 H2O 0.0002 g/l H3BO3 0.0001 g/l H2SeO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Double distilled water Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
8174	positive	growth	75
23130	positive	growth	65-85
23130	positive	optimum	75

Culture pH

@ref	Ability	Type	PH
23130	positive	maximum	7.8
23130	positive	minimum	4.8
23130	positive	optimum	5.9
23130	positive	growth	4.8-7.8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
8174	microaerophile
125439	anaerobe	98.7

Nutrition type

23130

Typechemolithotroph

Spore formation

@ref	Spore formation	Confidence
125438		94.728
125439		90.6

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23130	NaCl	positive	growth	0-0.4 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23130	73706 ChEBI	bromosuccinate	+	carbon source
23130		casamino acids	+	carbon source
23130	16947 ChEBI	citrate	+	carbon source
23130	17234 ChEBI	glucose	+	carbon source
23130	17306 ChEBI	maltose	+	carbon source
23130	37684 ChEBI	mannose	+	carbon source
23130		peptone	+	carbon source
23130	28017 ChEBI	starch	+	carbon source
23130		yeast extract	+	carbon source

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cardiolipin biosynthesis	100	7 of 7
66794	chorismate metabolism	100	9 of 9
66794	ppGpp biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	coenzyme A metabolism	100	4 of 4
66794	biotin biosynthesis	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	palmitate biosynthesis	95.45	21 of 22
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	lipoate biosynthesis	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	threonine metabolism	80	8 of 10
66794	photosynthesis	78.57	11 of 14
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	lipid A biosynthesis	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	acetate fermentation	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	vitamin B6 metabolism	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	reductive acetyl coenzyme A pathway	71.43	5 of 7
66794	starch degradation	70	7 of 10
66794	vitamin B1 metabolism	69.23	9 of 13
66794	acetoin degradation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	formaldehyde oxidation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	flavin biosynthesis	66.67	10 of 15
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	purine metabolism	64.89	61 of 94
66794	glutamate and glutamine metabolism	64.29	18 of 28
66794	heme metabolism	64.29	9 of 14
66794	pentose phosphate pathway	63.64	7 of 11
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	isoleucine metabolism	62.5	5 of 8
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	hydrogen production	60	3 of 5
66794	citric acid cycle	57.14	8 of 14
66794	propanol degradation	57.14	4 of 7
66794	pyrimidine metabolism	55.56	25 of 45
66794	aspartate and asparagine metabolism	55.56	5 of 9
66794	non-pathway related	55.26	21 of 38
66794	alanine metabolism	55.17	16 of 29
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	methionine metabolism	50	13 of 26
66794	phenylmercury acetate degradation	50	1 of 2
66794	gluconeogenesis	50	4 of 8
66794	ethanol fermentation	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	1,4-dihydroxy-6-naphthoate biosynthesis	50	3 of 6
66794	C4 and CAM-carbon fixation	50	4 of 8
66794	glycolysis	47.06	8 of 17
66794	urea cycle	46.15	6 of 13
66794	leucine metabolism	46.15	6 of 13
66794	arginine metabolism	45.83	11 of 24
66794	proline metabolism	45.45	5 of 11
66794	histidine metabolism	44.83	13 of 29
66794	d-mannose degradation	44.44	4 of 9
66794	tyrosine metabolism	42.86	6 of 14
66794	tryptophan metabolism	42.11	16 of 38
66794	oxidative phosphorylation	40.66	37 of 91
66794	lysine metabolism	40.48	17 of 42
66794	glycogen metabolism	40	2 of 5
66794	propionate fermentation	40	4 of 10
66794	ketogluconate metabolism	37.5	3 of 8
66794	cyanate degradation	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	degradation of hexoses	33.33	6 of 18
66794	nitrate assimilation	33.33	3 of 9
66794	CO2 fixation in Crenarchaeota	33.33	3 of 9
66794	cysteine metabolism	33.33	6 of 18
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	degradation of pentoses	32.14	9 of 28
66794	ascorbate metabolism	31.82	7 of 22
66794	degradation of sugar alcohols	31.25	5 of 16
66794	sulfate reduction	30.77	4 of 13
66794	polyamine pathway	30.43	7 of 23
66794	coenzyme M biosynthesis	30	3 of 10
66794	myo-inositol biosynthesis	30	3 of 10
66794	Entner Doudoroff pathway	30	3 of 10
66794	lipid metabolism	29.03	9 of 31
66794	butanoate fermentation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	glutathione metabolism	21.43	3 of 14

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Environmental	#Terrestrial	-
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
8174	terrestrial hot spring	Rincón de la Vieja volcano, south-western foothills	Costa Rica	CRI	Middle and South America

Taxonmaps

69479

Global distribution of 16S sequence AM260555 (>99% sequence identity) for Thermocrinis minervae subclade from Microbeatlas

Aquatic Samples	47
Soil Samples	3
Animal Samples	8
Plant Samples
Total Samples	58

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8174	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	IMG-taxon 2698536703 annotated assembly for Thermocrinis minervae DSM 19557	chromosome	GCA_900142435	381751.3	2698536703	381751	83.58

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8174	Thermocrinis minervae partial 16S rRNA gene, strain CR11	AM260555	1491		381751

GC content

@ref	GC-content (mol%)	Method
8174	40.3	thermal denaturation, midpoint method (Tm)
23130	40.3	Thermal denaturation, fluorometry

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Thermocrinis minervae DSM 19557
IMG: 2698536703

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	90.60	no
125439	motility	BacteriaNetⓘ	no	74.00	no
125439	gram_stain	BacteriaNetⓘ	negative	88.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermocrinis minervae strain DSM 19557
PATRIC: 381751.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.96	yes
125438	anaerobic	anaerobicⓘ	no	56.80	no
125438	spore-forming	spore-formingⓘ	no	94.73	no
125438	aerobic	aerobicⓘ	no	76.12	no
125438	thermophilic	thermophileⓘ	yes	77.30	no
125438	flagellated	motile2+ⓘ	no	59.47	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring.	Caldwell SL, Liu Y, Ferrera I, Beveridge T, Reysenbach AL	Int J Syst Evol Microbiol	10.1099/ijs.0.010496-0	2009

References

#8174	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19557
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23130	Sara L. Caldwell, Yitai Liu, Isabel Ferrera, Terry Beveridge, Anna-Louise Reysenbach: Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring. IJSEM 60: 338 - 343 2010 ( DOI 10.1099/ijs.0.010496-0 , PubMed 19651724 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Thermocrinis minervae (DSM 19557, ATCC BAA-1533, CR11)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900142435

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media