Thermocrinis minervae CR11 | Type strain | DSM 19557, ATCC BAA-1533 | BacDiveID:525

Strain identifier

BacDive ID: 525

Type strain:

Species: Thermocrinis minervae

Strain Designation: CR11

Culture col. no.: DSM 19557, ATCC BAA-1533

Strain history: <- A.-L. Reysenbach, Dept. Biology, Portland State Univ., USA; CR11

NCBI tax ID(s): 381751 (species)

SI-ID 405654

DSM 19557

Special Collections

Section

Thermocrinis minervae CR11 is a microaerophile, chemolithotroph, Gram-negative bacterium that was isolated from terrestrial hot spring.

Gram-negative
motile
rod-shaped
chemolithotroph
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Aquificota*
	Class	*Aquificia*
	Order	*Aquificales*
	Family	*Aquificaceae*
	Genus	*Thermocrinis*
	Species	**Thermocrinis minervae**
	Full Scientific Name (LPSN)	Thermocrinis minervae Caldwell et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #23130]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98.955

[Ref.: #23130]	Cell length	2.4-3.9 µm

[Ref.: #23130]	Cell width	0.5-0.6 µm

[Ref.: #23130]	Cell shape	rod-shaped

[Ref.: #23130]	Motility	yes

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

MS-MEDIUM (MODIFIED) (DSMZ Medium 1145)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8174]			growth	75 °C
[Ref.: #23130]			growth	65-85 °C
[Ref.: #23130]			optimum	75 °C

	pH	Kind of pH		pH
[Ref.: #23130]			maximum	7.8
[Ref.: #23130]			minimum	4.8
[Ref.: #23130]			optimum	5.9
[Ref.: #23130]			growth	4.8-7.8

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

microaerophile

Nutrition type

chemolithotroph

Ability of spore formation

no Confidence in %94.728

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23130]		NaCl		growth	0-0.4	%(w/v)

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23130]		bromosuccinate ChEBI	+	carbon source
[Ref.: #23130]		casamino acids	+	carbon source
[Ref.: #23130]		citrate ChEBI	+	carbon source
[Ref.: #23130]		glucose ChEBI	+	carbon source
[Ref.: #23130]		maltose ChEBI	+	carbon source
[Ref.: #23130]		mannose ChEBI	+	carbon source
[Ref.: #23130]		peptone	+	carbon source
[Ref.: #23130]		starch ChEBI	+	carbon source
[Ref.: #23130]		yeast extract	+	carbon source

	Pathway	Enzyme coverage	Annotated reactions	External links
	Pathway annotation	Computationally determined by
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	chorismate metabolism	100	9 of 9
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	ubiquinone biosynthesis	85.71	6 of 7
[Ref.: #66794]	lipoate biosynthesis	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	78.57	11 of 14
[Ref.: #66794]	lipid A biosynthesis	77.78	7 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	vitamin B6 metabolism	72.73	8 of 11
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	reductive acetyl coenzyme A pathway	71.43	5 of 7
[Ref.: #66794]	starch degradation	70	7 of 10
[Ref.: #66794]	vitamin B1 metabolism	69.23	9 of 13
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	flavin biosynthesis	66.67	10 of 15
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	purine metabolism	64.89	61 of 94
[Ref.: #66794]	glutamate and glutamine metabolism	64.29	18 of 28
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	pentose phosphate pathway	63.64	7 of 11
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	isoleucine metabolism	62.5	5 of 8
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	citric acid cycle	57.14	8 of 14
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	pyrimidine metabolism	55.56	25 of 45
[Ref.: #66794]	aspartate and asparagine metabolism	55.56	5 of 9
[Ref.: #66794]	non-pathway related	55.26	21 of 38
[Ref.: #66794]	alanine metabolism	55.17	16 of 29
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	methionine metabolism	50	13 of 26
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	gluconeogenesis	50	4 of 8
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	1,4-dihydroxy-6-naphthoate biosynthesis	50	3 of 6
[Ref.: #66794]	C4 and CAM-carbon fixation	50	4 of 8
[Ref.: #66794]	glycolysis	47.06	8 of 17
[Ref.: #66794]	urea cycle	46.15	6 of 13
[Ref.: #66794]	leucine metabolism	46.15	6 of 13
[Ref.: #66794]	arginine metabolism	45.83	11 of 24
[Ref.: #66794]	proline metabolism	45.45	5 of 11
[Ref.: #66794]	histidine metabolism	44.83	13 of 29
[Ref.: #66794]	d-mannose degradation	44.44	4 of 9
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	tryptophan metabolism	42.11	16 of 38
[Ref.: #66794]	oxidative phosphorylation	40.66	37 of 91
[Ref.: #66794]	lysine metabolism	40.48	17 of 42
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	propionate fermentation	40	4 of 10
[Ref.: #66794]	ketogluconate metabolism	37.5	3 of 8
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	degradation of hexoses	33.33	6 of 18
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	33.33	3 of 9
[Ref.: #66794]	cysteine metabolism	33.33	6 of 18
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
[Ref.: #66794]	degradation of pentoses	32.14	9 of 28
[Ref.: #66794]	ascorbate metabolism	31.82	7 of 22
[Ref.: #66794]	degradation of sugar alcohols	31.25	5 of 16
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	polyamine pathway	30.43	7 of 23
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	Entner Doudoroff pathway	30	3 of 10
[Ref.: #66794]	lipid metabolism	29.03	9 of 31
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	glutathione metabolism	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8174]	Sample type/isolated from	terrestrial hot spring
[Ref.: #8174]	Geographic location (country and/or sea, region)	Rincón de la Vieja volcano, south-western foothills
[Ref.: #8174]	Country	Costa Rica
[Ref.: #8174]	Country ISO 3 Code	CRI
[Ref.: #8174]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Environmental	#Terrestrial	-
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Global distribution of 16S sequence AM260555 (>99% sequence identity) for Thermocrinis minervae subclade from Microbeatlas

Aquatic Samples	47
Soil Samples	3
Animal Samples	8
Plant Samples
Total Samples	58

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

1

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8174]	Thermocrinis minervae partial 16S rRNA gene, strain CR11	AM260555	1491	GenBank ENA	381751 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermocrinis minervae DSM 19557	GCA_900142435	chromosome	GenBank ENA	381751 tax ID	*
[Ref.: #66792]	Thermocrinis minervae strain DSM 19557	381751.3	complete	PATRIC	381751 tax ID	*
[Ref.: #66792]	Thermocrinis minervae DSM 19557	2698536703	draft	JGI IMG/M	381751 tax ID	*

[Ref.: #8174]	GC-content	40.3 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #23130]	GC-content	40.3 mol%	Thermal denaturation, fluorometry

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermocrinis minervae strain DSM 19557 PATRIC: 381751.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	51	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermocrinis minervae strain DSM 19557 PATRIC: 381751.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.955	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	56.803	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	94.728	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	76.119	no
[Ref.: #69480]	thermophilic	thermophileⓘ	yes	77.298	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	59.465	no

Availability in culture collections External links

Culture collection no.

DSM 19557, ATCC BAA-1533

SI-ID 405654

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring.	Caldwell SL, Liu Y, Ferrera I, Beveridge T, Reysenbach AL	Int J Syst Evol Microbiol	10.1099/ijs.0.010496-0	2009	*

References

#8174

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19557

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23130

Sara L. Caldwell, Yitai Liu, Isabel Ferrera, Terry Beveridge, Anna-Louise Reysenbach: Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring. IJSEM 60: 338 - 343 2010 ( DOI 10.1099/ijs.0.010496-0 , PubMed 19651724 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70203

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405654.1 )

* These data were automatically processed and therefore are not curated

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