Name: Cronobacter malonaticus API 76-2121
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5237

Type strain:

Species: Cronobacter malonaticus

Strain Designation: API 76-2121, CDC 1058-77, E825

Culture col. no.: CCUG 56035, DSM 18702, LMG 23826

Strain history: <- A. Lehner; E825 <- Nestec, S.A. <- CDC <- Analytabs Products Inc. Plainview, New York, USA

NCBI tax ID(s): 1159491 (strain), 413503 (species)

Section

Cronobacter malonaticus API 76-2121 is a facultative anaerobe, mesophilic, gram-negative bacterium that was isolated from breast abscess.

gram-negative
motile
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	23-11-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Proteobacteria
	Class	Gammaproteobacteria
	Order	Enterobacterales
	Family	Enterobacteriaceae
	Genus	Cronobacter
	Species	Cronobacter malonaticus
	Full Scientific Name (PNU)	Cronobacter malonaticus Iversen et al. 2008

Synonym

Cronobacter sakazakii subsp. malonaticus

Information on morphological and physiological properties Morphology

[Ref.: #32494]	Gram stain	negative
[Ref.: #32494]	Cell length	3 µm
[Ref.: #32494]	Cell width	1 µm
[Ref.: #32494]	Cell shape	rod-shaped
[Ref.: #32494]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7663]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #7663]

Culture medium growth

[Ref.: #7663]

Culture medium link

Medium recipe at BacMedia

[Ref.: #7663]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7663]	growth	37 °C
[Ref.: #32494]	growth	06-45 °C
[Ref.: #60835]	growth	30 °C

[Ref.: #7663]	Temperature range	mesophilic
[Ref.: #60835]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32494]			growth	05-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32494]	Oxygen tolerance	facultative anaerobe
[Ref.: #60835]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32494]		NaCl		growth	<7	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32494]	esculin ChEBI	+	hydrolysis
[Ref.: #32494]	malonate ChEBI	+	carbon source
[Ref.: #32494]	nitrate ChEBI	+	reduction
[Ref.: #32494]	putrescine ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #32494]		catalase	+	1.11.1.6

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #7663]	9281	+	+	-	+	+	-	-	-	-	+	-	+	+	-	-	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7663]	Sample type/isolated from	breast abscess
[Ref.: #7663]	Country	USA
[Ref.: #7663]	Country ISO 3 Code	USA
[Ref.: #7663]	Continent	North America

[Ref.: #60835]	Sample type/isolated from	Human breast abscess
[Ref.: #60835]	Geographic location (country and/or sea, region)	New York,Plainview
[Ref.: #60835]	Country	USA
[Ref.: #60835]	Country ISO 3 Code	USA
[Ref.: #60835]	Continent	North America

Isolation sources categories

#Host Body-Site	#Other	#Abscess
#Host Body-Site	#Other	#Thoracic segment

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7663]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #32494]	Cronobacter malonaticus strain E825 16S ribosomal RNA gene, partial sequence	EF059881	1417	GenBank	413503 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cronobacter malonaticus LMG 23826	GCA_001277215	complete	GenBank	1159491 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus CMCC45402	GCA_000504545	complete	GenBank	413503 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus LMG 23826	GCA_000409305	contig	GenBank	1159491 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus cro2841W	GCA_002978325	contig	GenBank	413503 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus cro864B2-3	GCA_002983565	contig	GenBank	413503 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus CMa3	GCA_013186065	contig	GenBank	413503 tax ID	*

Availability in culture collections External links

[Ref.: #7663]

Culture collection no.

CCUG 56035, DSM 18702, LMG 23826

[Ref.: #20218]

Associated Passport(s) in StrainInfo

823193, 823194

References

#7663

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18702

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32494

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 ) - originally annotated from #28714 (see below)

#60835 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 56035

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#28714

IJSEM 1442 2008 ( DOI 10.1099/ijs.0.65577-0 )

* These References are textmined

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