Name: Cronobacter malonaticus E825
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 5237

Type strain:

Species: Cronobacter malonaticus

Strain Designation: E825

Culture col. no.: CCUG 56035, DSM 18702, LMG 23826, API 76-2121, CDC 1058-77

Strain history: <- A. Lehner; E825 <- Nestec, S.A. <- CDC <- Analytabs Products Inc. Plainview, New York, USA

NCBI tax ID(s): 1159491 (strain), 413503 (species)

SI-ID 354307

DSM 18702, LMG 23826, CCUG 56035

Special Collections

Section

When using BacDive for research please cite our paper

Cronobacter malonaticus E825 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from breast abscess.

Gram-negative
motile
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Cronobacter*
	Species	**Cronobacter malonaticus**
	Full Scientific Name (LPSN)	Cronobacter malonaticus Iversen et al. 2008

Synonym

"Cronobacter sakazakii subsp. malonaticus"

Information on morphological and physiological properties Morphology

[Ref.: #32494]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %99.5
[Ref.: #125439]	Gram stain	negative Confidence in %99.2

[Ref.: #32494]	Cell length	3 µm

[Ref.: #32494]	Cell width	1 µm

[Ref.: #32494]	Cell shape	rod-shaped

[Ref.: #32494]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7663]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #7663]

Culture medium growth

[Ref.: #7663]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7663]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7663]	growth	37 °C
[Ref.: #32494]	growth	06-45 °C
[Ref.: #60835]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #32494]			growth	05-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32494]	Oxygen tolerance	facultative anaerobe
[Ref.: #60835]	Oxygen tolerance	aerobe
[Ref.: #125439]	Oxygen tolerance	aerobe Confidence in %92.6

[Ref.: #125439]

Ability of spore formation

no Confidence in %96.6

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32494]		NaCl		growth	<7	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #32494]	esculin ChEBI	+	hydrolysis
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #32494]	malonate ChEBI	+	carbon source
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #32494]	nitrate ChEBI	+	reduction
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #32494]	putrescine ChEBI	+	carbon source
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #32494]	catalase	+	1.11.1.6
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #7663]	18286	+	+	-	+	+	-	-	-	-	+	-	+	+	-	-	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7663]	Sample type/isolated from	breast abscess
[Ref.: #7663]	Country	USA
[Ref.: #7663]	Country ISO 3 Code	USA
[Ref.: #7663]	Continent	North America

[Ref.: #60835]	Sample type/isolated from	Human breast abscess
[Ref.: #60835]	Geographic location (country and/or sea, region)	New York,Plainview
[Ref.: #60835]	Country	USA
[Ref.: #60835]	Country ISO 3 Code	USA
[Ref.: #60835]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Other	#Abscess
#Host Body-Site	#Other	#Thoracic segment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF059881 (>99% sequence identity) for Cronobacter from Microbeatlas

Aquatic Samples	140
Soil Samples	36
Animal Samples	1436
Plant Samples	92
Total Samples	1704

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7663]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #32494]	Cronobacter malonaticus strain E825 16S ribosomal RNA gene, partial sequence	EF059881	1417	GenBank ENA	413503 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cronobacter malonaticus LMG 23826 GCA_001277215.2	2827186723	complete	JGI IMG/M	1159491 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus LMG 23826	GCA_000409305	contig	GenBank ENA	1159491 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus LMG 23826	1159491.20	plasmid	PATRIC	1159491 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus LMG 23826	1159491.6	complete	PATRIC	1159491 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus LMG 23826	1159491.19	plasmid	PATRIC	1159491 tax ID	*
[Ref.: #66792]	Cronobacter malonaticus LMG 23826	1159491.3	wgs	PATRIC	1159491 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Cronobacter malonaticus LMG 23826 LMG 23826 NCBI: GCA_001277215.2
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	99.5	yes
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	96.024	no
[Ref.: #125438]	aerobic	aerobicⓘ	no	60.553	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	88.804	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	99.3	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	75.089	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Cronobacter malonaticus LMG 23826 NCBI: GCA_001277215
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	aerobe	92.6
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	99.2
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	59.5
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	96.6

Availability in culture collections External links

[Ref.: #7663]

Culture collection no.

CCUG 56035, DSM 18702, LMG 23826, API 76-2121, CDC 1058-77

[Ref.: #74728]

SI-ID 354307

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.	Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten H	Int J Syst Evol Microbiol	10.1099/ijs.0.65577-0	2008	*

References

#7663

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18702

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32494

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28714 (see below)

#60835 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 56035

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#74728

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID354307.1 )

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

#28714

IJSEM 1442 2008 ( DOI 10.1099/ijs.0.65577-0 , PubMed 18523192 )

* These data were automatically processed and therefore are not curated

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Cronobacter malonaticus LMG 23826 LMG 23826

Cronobacter malonaticus LMG 23826

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