Name: Thorsellia anophelis DSM 18579
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5235

Type strain:

Species: Thorsellia anophelis

Culture col. no.: DSM 18579, CCUG 49520, CIP 108754

Strain history: CIP <- 2005, P. Kämpfer, Giessen Univ., Giessen, Germany <- J. Lindh, Stockholm Univ., Stockholm, Sweden: strain 49520

NCBI tax ID(s): 1123402 (strain), 336804 (species)

SI-ID 289986

CCUG 49520, CIP 108754, DSM 18579

Section

Thorsellia anophelis DSM 18579 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from stomach of Anopheles gambiae.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	[Gammaproteobacteria, not assigned to order]
	Family	*Thorselliaceae*
	Genus	*Thorsellia*
	Species	**Thorsellia anophelis**
	Full Scientific Name (LPSN)	Thorsellia anophelis Kämpfer et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31664]	Gram stain	negative
[Ref.: #116400]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.995

[Ref.: #31664]	Cell shape	rod-shaped
[Ref.: #116400]	Cell shape	rod-shaped

[Ref.: #31664]	Motility	yes
[Ref.: #116400]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %93.147

[Ref.: #58594]

Incubation period

2 days

[Ref.: #31664]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7537]

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

[Ref.: #7537]

Culture medium growth

[Ref.: #7537]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7537]

Culture medium composition

expand / minimize

[Ref.: #32802]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #32802]

Culture medium growth

[Ref.: #32802]

Culture medium composition

expand / minimize

[Ref.: #116400]

Culture medium

CIP Medium 72

[Ref.: #116400]

Culture medium growth

[Ref.: #116400]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7537]	growth	30 °C
[Ref.: #31664]	growth	15-45 °C
[Ref.: #31664]	optimum	30 °C
[Ref.: #32802]	growth	37 °C
[Ref.: #58594]	growth	37 °C

[Ref.: #7537]	Temperature range	mesophilic
[Ref.: #31664]	Temperature range	mesophilic
[Ref.: #32802]	Temperature range	mesophilic
[Ref.: #58594]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31664]			optimum	7
[Ref.: #31664]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31664]	Oxygen tolerance	aerobe
[Ref.: #58594]	Oxygen tolerance	aerobe
[Ref.: #116400]	Oxygen tolerance	facultative anaerobe

[Ref.: #31664]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.978

[Ref.: #31664]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31664]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #31664]	adipate ChEBI	+	carbon source
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE, API 20E, API ID32E
[Ref.: #68374]	cellobiose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68374]	D-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68377]	D-fructose ChEBI	+	builds acid from	* from API NH
[Ref.: #68374]	D-galacturonic acid ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68377]	D-glucose ChEBI	+	builds acid from	* from API NH
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE, API 20E
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	D-glucose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	D-mannitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #31664]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #31664]	gluconate ChEBI	+	carbon source
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #31664]	glucose ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	L-arabinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68374]	L-arabitol ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68374]	L-rhamnose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #31664]	lactate ChEBI	+	carbon source
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E, API ID32E
[Ref.: #31664]	malate ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	malonate ChEBI	-	assimilation	* from API ID32E
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68374]	maltose ChEBI	+	builds acid from	* from API ID32E, API NH
[Ref.: #31664]	mannitol ChEBI	+	carbon source
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68374]	myo-inositol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #116400]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #116400]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E, API ID32E, API NH
[Ref.: #68374]	palatinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API ID32E
[Ref.: #31664]	propionate ChEBI	+	carbon source
[Ref.: #68374]	ribitol ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68374]	sorbitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #31664]	sucrose ChEBI	+	carbon source
[Ref.: #68377]	sucrose ChEBI	+	builds acid from	* from API NH
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	sucrose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #31664]	trehalose ChEBI	+	carbon source
[Ref.: #68374]	trehalose ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE, API 20E, API ID32E, API NH
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API 20E, API ID32E, API NH
[Ref.: #31664]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #116400]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68377]	indole ChEBI	no	* from API NH, API ID32E, API 20NE, API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68377]		indole ChEBI		-	* from API NH, API ID32E, API 20NE, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #116400]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API NH
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym, API ID32E
[Ref.: #68374]	alpha-glucosidase	-	3.2.1.20	* from API ID32E
[Ref.: #68374]	alpha-maltosidase	-		* from API ID32E
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68374]	arginine dihydrolase	-	3.5.3.6	* from API ID32E, API 20NE, API 20E
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API NH, API ID32E, API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API ID32E, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API ID32E
[Ref.: #68377]	beta-lactamase	-	3.5.2.6	* from API NH
[Ref.: #116400]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE, API 20E
[Ref.: #68377]	gamma-glutamyltransferase	-	2.3.2.2	* from API NH
[Ref.: #68369]	gelatinase	-		* from API 20NE, API 20E
[Ref.: #68374]	L-aspartate arylamidase	-	3.4.11.21	* from API ID32E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68377]	lipase	-		* from API NH, API ID32E
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116400]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68374]	lysine decarboxylase	-	4.1.1.18	* from API ID32E, API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API ID32E
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116400]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH, API ID32E, API 20E
[Ref.: #116400]	oxidase	-
[Ref.: #68377]	proline-arylamidase	-	3.4.11.5	* from API NH
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH, API 20E
[Ref.: #116400]	urease	-	3.5.1.5
[Ref.: #68377]	urease	-	3.5.1.5	* from API NH, API ID32E, API 20NE, API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #58594]

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	39.1	17.824
C16:0	24.3	16
C16:1 ω7c	15.1	15.819
C14:0	11.2	14
C14:0 3OH/C16:1 ISO I	4.5	15.485
C12:0 ALDE ?	1.4	10.915
unk. 18.846/19:1 ω6c	1.3	18.846
C13:0 ISO 2OH	0.6	13.814
C18:0	0.6	18
unknown 14.503	0.6	14.503
C12:0 3OH	0.5	13.455
C16:1 ω5c	0.4	15.908
C15:0	0.3	15
Unidentified	0.3	15.175

API® 20E: