Xenorhabdus ehlersii (DSM 16337, CIP 108893, NCIMB 14018)

Name: Xenorhabdus ehlersii (DSM 16337, CIP 108893, NCIMB 14018)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5191

Type strain

Strain Designation Ser

Culture col. no. DSM 16337 CIP 108893 NCIMB 14018 Ser

NCBI tax ID(s) 290111

Special Collections DSMZ CIP

Links

Xenorhabdus ehlersii Ser is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from nematode Steinernema serratum.

Gram-negative motile rod-shaped facultative anaerobe mesophilic animal pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 132358

DSM 16337,NCIMB 14018,CIP 108893

@ref 20215

Last LPSN update 2025-12-16 09:48:00

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Enterobacterales

Family Morganellaceae

Genus Xenorhabdus

Species Xenorhabdus ehlersii

Full scientific name Xenorhabdus ehlersii Lengyel et al. 2005

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
119001	negative	rod-shaped
125438	negative			100

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6394	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
38359	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
38360	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119001	CIP Medium 72	Medium recipe at CIP
6394	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
6394	TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)	Medium recipe at MediaDive	Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6394	positive	growth	30	mesophilic
6394	positive	growth	28
38359	positive	growth	25	mesophilic
38360	positive	growth	25	mesophilic
119001	positive	growth	30-37	mesophilic
119001	negative	growth	5
119001	negative	growth	10
119001	negative	growth	41

Physiology and metabolism

Oxygen tolerance

119001

Oxygen tolerancefacultative anaerobe

Spore formation

@ref	Spore formation	Confidence
125439		95.6
125438		92.72

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
119001	16947 ChEBI	citrate	+	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
119001	17234 ChEBI	glucose	+	fermentation
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
119001	17716 ChEBI	lactose	-	fermentation
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
119001	15792 ChEBI	malonate	-	assimilation
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
119001	29864 ChEBI	mannitol	-	fermentation
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
119001	17632 ChEBI	nitrate	-	reduction
119001	17632 ChEBI	nitrate	+	respiration
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
119001	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
119001	132112 ChEBI	sodium thiosulfate	-	builds gas from
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
119001	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test	Methylred-test
119001	17234 ChEBI	glucose		-
68369	35581 ChEBI	indole	-		from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
119001	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
119001	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119001	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119001	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
119001	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119001	ornithine decarboxylase	-	4.1.1.17
119001	oxidase	-
119001	phenylalanine ammonia-lyase	+	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
119001	tryptophan deaminase	+
119001	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119001	-	+	+	+	-	+	+	+	-	-	+	+	-	+	-	+	+	+	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
6394	-	-	-	+/-	-	+	+	-	+	-	+	-	+	+	+	-	-	+	+	-	+/-
6394	-	-	+	+	+	+	+	-	+	-	+	-	+	+	+	-	-	+	+	+	-
6394	-	-	+	+	+	+	-	-	+	-	+	-	+	+	+	-	-	+	+	+	-
6394	-	-	+	+	+	+	+	-	+	-	+	-	+	+	+	-	-	-	+	+	+

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
119001	not determinedn.d.	+/-	-	-	-	+/-	-	-	-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	-	-	+/-	-	-	+/-	-	-	+/-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Invertebrates (Other)	#Nematoda

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent	Geographic location
6394	nematode Steinernema serratum	Steinernema serratum	China	CHN	Asia
119001	Other, Nematode Steinernema serratum		China	CHN	Asia	Southern China

Taxonmaps

69479

Global distribution of 16S sequence AJ810294 (>99% sequence identity) for Xenorhabdus ehlersii from Microbeatlas

Aquatic Samples
Soil Samples
Animal Samples	3
Plant Samples
Total Samples	3

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
6394		1	Risk group (German classification) According to TRBA 466
119001		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM361046v1 assembly for Xenorhabdus ehlersii DSM 16337	contig	GCA_003610465	290111.6	2772190835	290111	72.49
66792	ASM263244v1 assembly for Xenorhabdus ehlersii DSM 16337	scaffold	GCA_002632445	290111.5		290111	36.49

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Xenorhabdus ehlersii partial 16S rRNA gene, type strain DSM 16337T	AJ810294	1524		290111

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Xenorhabdus ehlersii DSM 16337
NCBI: GCA_002632445

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	95.60	no
125439	motility	BacteriaNetⓘ	no	55.40	no
125439	gram_stain	BacteriaNetⓘ	negative	77.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	75.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Xenorhabdus ehlersii DSM 16337
NCBI: GCA_002632445.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	100.00	no
125438	anaerobic	anaerobicⓘ	no	98.48	no
125438	aerobic	aerobicⓘ	no	66.11	no
125438	spore-forming	spore-formingⓘ	no	92.72	no
125438	thermophilic	thermophileⓘ	no	98.38	yes
125438	flagellated	motile2+ⓘ	yes	75.57	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Whole-Genome Sequencing and Biosynthetic Gene Cluster Analysis of Novel Entomopathogenic Bacteria Xenorhabdus thailandensis ALN 7.1 and ALN 11.5.	Meesil W, Ardpairin J, Sharkey LKR, Pidot SJ, Vitta A, Thanwisai A.	Biology (Basel)	10.3390/biology14080905	2025
Genetics	Genome announcement of Steinernema khuongi and its associated symbiont from Florida.	Baniya A, DiGennaro P.	G3 (Bethesda)	10.1093/g3journal/jkab053	2021
	A Photorhabdus natural product inhibits insect juvenile hormone epoxide hydrolase.	Nollmann FI, Heinrich AK, Brachmann AO, Morisseau C, Mukherjee K, Casanova-Torres AM, Strobl F, Kleinhans D, Kinski S, Schultz K, Beeton ML, Kaiser M, Chu YY, Phan Ke L, Thanwisai A, Bozhuyuk KA, Chantratita N, Gotz F, Waterfield NR, Vilcinskas A, Stelzer EH, Goodrich-Blair H, Hammock BD, Bode HB.	Chembiochem	10.1002/cbic.201402650	2015
Enzymology	Proteolytic enzyme production by strains of the insect pathogen xenorhabdus and characterization of an early-log-phase-secreted protease as a potential virulence factor.	Massaoud MK, Marokhazi J, Fodor A, Venekei I.	Appl Environ Microbiol	10.1128/aem.01567-10	2010
Phylogeny	Xenorhabdus thuongxuanensis sp. nov. and Xenorhabdus eapokensis sp. nov., isolated from Steinernema species.	Kampfer P, Tobias NJ, Ke LP, Bode HB, Glaeser SP	Int J Syst Evol Microbiol	10.1099/ijsem.0.001770	2017
Phylogeny	Xenorhabdus ishibashii sp. nov., isolated from the entomopathogenic nematode Steinernema aciari.	Kuwata R, Qiu LH, Wang W, Harada Y, Yoshida M, Kondo E, Yoshiga T	Int J Syst Evol Microbiol	10.1099/ijs.0.041145-0	2012
Phylogeny	Description of four novel species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus budapestensis sp. nov., Xenorhabdus ehlersii sp. nov., Xenorhabdus innexi sp. nov., and Xenorhabdus szentirmaii sp. nov.	Lengyel K, Lang E, Fodor A, Szallas E, Schumann P, Stackebrandt E	Syst Appl Microbiol	10.1016/j.syapm.2004.10.004	2005

References

#6394	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16337
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38359	; Curators of the CIP;
#38360	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119001	Collection of Institut Pasteur ; Curators of the CIP; CIP 108893
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Xenorhabdus ehlersii (DSM 16337, CIP 108893, NCIMB 14018)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003610465

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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