Name: Mixta calida 1400/07
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5012

Type strain:

Species: Mixta calida

Strain Designation: 1400/07

Culture col. no.: DSM 22759, LMG 25383

Strain history: <- R. Stephan, Inst. for Food Safety and Hygiene, Univ. Zurich, Switzerland; 1400/07

NCBI tax ID(s): 665913 (species)

SI-ID 356255

LMG 25383, DSM 22759, CCUG 76605

Section

Mixta calida 1400/07 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from powdered infant formula.

Gram-negative
motile
ovoid-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Erwiniaceae*
	Genus	*Mixta*
	Species	**Mixta calida**
	Full Scientific Name (LPSN)	Mixta calida (Popp et al. 2010) Palmer et al. 2018

Synonym

Pantoea calida

Information on morphological and physiological properties Morphology

[Ref.: #29720]	Gram stain	negative

[Ref.: #29720]	Cell length	1.5-2 µm

[Ref.: #29720]	Cell width	1 µm

[Ref.: #29720]	Cell shape	ovoid-shaped

[Ref.: #29720]	Motility	yes

[Ref.: #29720]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16499]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #16499]

Culture medium growth

[Ref.: #16499]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16499]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16499]	growth	37 °C
[Ref.: #29720]	growth	10-44 °C
[Ref.: #29720]	optimum	37-44 °C

[Ref.: #16499]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29720]

Oxygen tolerance

facultative anaerobe

[Ref.: #29720]

Ability of spore formation

[Ref.: #29720]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #29720]	esculin ChEBI	+	hydrolysis
[Ref.: #29720]	fructose ChEBI	+	carbon source
[Ref.: #29720]	galactose ChEBI	+	carbon source
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #29720]	glucose ChEBI	+	carbon source
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #29720]	lactose ChEBI	+	carbon source
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #29720]	maltose ChEBI	+	carbon source
[Ref.: #29720]	mannose ChEBI	+	carbon source
[Ref.: #29720]	melibiose ChEBI	+	carbon source
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #29720]	raffinose ChEBI	+	carbon source
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #29720]	sucrose ChEBI	+	carbon source
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #29720]	trehalose ChEBI	+	carbon source
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68368]		hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]		indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Indole test
[Ref.: #68368]		indole ChEBI	-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #29720]	catalase	+	1.11.1.6
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #16499]	17795	+	+	-	-	+	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #16499]	17785	-	-	-	-	+	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16499]	Sample type/isolated from	powdered infant formula
[Ref.: #16499]	Geographic location (country and/or sea, region)	Zurich
[Ref.: #16499]	Country	Switzerland
[Ref.: #16499]	Country ISO 3 Code	CHE
[Ref.: #16499]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Food

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GQ367478 (>99% sequence identity) for Pantoea from Microbeatlas

Aquatic Samples	334
Soil Samples	151
Animal Samples	2011
Plant Samples	659
Total Samples	3155

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16499]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16499]	Pantoea calida strain 1400/07 16S ribosomal RNA gene, partial sequence	GQ367478	1495	ENA	665913 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mixta calida DSM 22759	GCA_002953215	complete	GenBank	665913 tax ID	*
[Ref.: #66792]	Mixta calida LMG 25383	GCA_002095355	contig	GenBank	665913 tax ID	*
[Ref.: #66792]	Pantoea calida strain LMG 25383	665913.3	wgs	PATRIC	665913 tax ID	*
[Ref.: #66792]	Mixta calida LMG 25383	2791355158	draft	JGI IMG/M	665913 tax ID	*
[Ref.: #66792]	Mixta calida DSM 22759	2791355150	complete	JGI IMG/M	665913 tax ID	*

[Ref.: #16499]	GC-content	57.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29720]	GC-content	57.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mixta calida DSM 22759 NCBI: GCA_002953215.1
[Ref.: #69480]	flagellated	no	71.736	no
[Ref.: #69480]	gram-positive	no	97.765	yes
[Ref.: #69480]	anaerobic	no	98.629	no
[Ref.: #69480]	aerobic	no	75.39	yes
[Ref.: #69480]	halophile	no	80.507	no
[Ref.: #69480]	spore-forming	no	94.075	yes
[Ref.: #69480]	glucose-util	yes	95.33	no
[Ref.: #69480]	motile	yes	93.471	yes
[Ref.: #69480]	glucose-ferment	yes	90.95	no
[Ref.: #69480]	thermophile	no	98.859	yes

Availability in culture collections External links

[Ref.: #16499]

Culture collection no.

DSM 22759, LMG 25383

[Ref.: #74507]

SI-ID 356255

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mixta tenebrionis sp. nov., isolated from the gut of the plastic-eating mealworm Tenebrio molitor L.	Xia M, Wang J, Huo YX, Yang Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.003826	2020	*
Genetics	Complete and Assembled Genome Sequences of Pantoea calida DSM 22759(T) and Pantoea gaviniae DSM 22758(T).	Stevens MJA, Zurfluh K, Stephan R	Genome Announc	10.1128/genomeA.00157-18	2018	*
Phylogeny	Pantoea intestinalis sp. nov., isolated from the human gut.	Prakash O, Nimonkar Y, Vaishampayan A, Mishra M, Kumbhare S, Josef N, Shouche YS	Int J Syst Evol Microbiol	10.1099/ijsem.0.000419	2015	*
Phylogeny	Pantoea gaviniae sp. nov. and Pantoea calida sp. nov., isolated from infant formula and an infant formula production environment.	Popp A, Cleenwerck I, Iversen C, De Vos P, Stephan R	Int J Syst Evol Microbiol	10.1099/ijs.0.019430-0	2010	*

References

#16499

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22759

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29720

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26103 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#74507

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID356255.1 )

#26103

IJSEM 2786 2010 ( DOI 10.1099/ijs.0.019430-0 , PubMed 20061487 )

* These data were automatically processed and therefore are not curated

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Mixta calida DSM 22759

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