Mixta calida (DSM 22759, LMG 25383, 1400/07)

Name: Mixta calida (DSM 22759, LMG 25383, 1400/07)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5012

Type strain

Strain Designation 1400/07

Culture col. no. DSM 22759 LMG 25383 1400/07

NCBI tax ID(s) 665913

Special Collections DSMZ Literature strains

History <- R. Stephan, Inst. for Food Safety and Hygiene, Univ. Zurich, Switzerland; 1400/07

Links

Mixta calida 1400/07 is a facultative anaerobe, Gram-negative, motile bacterium that was isolated from powdered infant formula.

Gram-negative motile ovoid-shaped facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 356255

LMG 25383,DSM 22759,CCUG 76605

@ref 20215

Last LPSN update 2026-02-09 11:18:52

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Enterobacterales

Family Erwiniaceae

Genus Mixta

Species Mixta calida

Full scientific name Mixta calida (Popp et al. 2010) Palmer et al. 2018

Synonyms (1)

Pantoea calida

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
29720	negative	1.5-2 µm	1 µm	ovoid-shaped
125439	negative				98.3
125438	negative				99.5

Pigmentation

29720

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16499	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)		Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
16499	positive	growth	37
29720	positive	growth	10-44
29720	positive	optimum	37-44

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
29720	facultative anaerobe
125439	aerobe	98.3

Spore formation

@ref	Spore formation	Confidence
29720
125439		94.4

Observation

29720

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	27613 ChEBI	amygdalin	+	fermentation	from API 20E
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68368	17634 ChEBI	D-glucose	+	fermentation	from API 20E
68368	16899 ChEBI	D-mannitol	+	fermentation	from API 20E
29720	4853 ChEBI	esculin	+	hydrolysis
29720	28757 ChEBI	fructose	+	carbon source
29720	28260 ChEBI	galactose	+	carbon source
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
29720	17234 ChEBI	glucose	+	carbon source
68368	30849 ChEBI	L-arabinose	+	fermentation	from API 20E
68368	62345 ChEBI	L-rhamnose	+	fermentation	from API 20E
29720	17716 ChEBI	lactose	+	carbon source
68368	25094 ChEBI	lysine	-	degradation	from API 20E
29720	17306 ChEBI	maltose	+	carbon source
29720	37684 ChEBI	mannose	+	carbon source
29720	28053 ChEBI	melibiose	+	carbon source
68368	28053 ChEBI	melibiose	+	fermentation	from API 20E
68368	17268 ChEBI	myo-inositol	+	fermentation	from API 20E
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
29720	16634 ChEBI	raffinose	+	carbon source
68368	30911 ChEBI	sorbitol	+	fermentation	from API 20E
29720	17992 ChEBI	sucrose	+	carbon source
68368	17992 ChEBI	sucrose	+	fermentation	from API 20E
29720	27082 ChEBI	trehalose	+	carbon source
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68368	35581 ChEBI	indole	-	from API 20E

Enzymes

@ref	Value	Activity	Ec
29720	catalase	+	1.11.1.6
68368	cytochrome oxidase	-	1.9.3.1	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
16499	+	+	-	-	+	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
16499	-	-	-	-	+	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Food

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
16499	powdered infant formula	Zurich	Switzerland	CHE	Europe

Taxonmaps

69479

Global distribution of 16S sequence GQ367478 (>99% sequence identity) for Pantoea from Microbeatlas

Aquatic Samples	334
Soil Samples	151
Animal Samples	2011
Plant Samples	659
Total Samples	3155

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16499	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM295321v1 assembly for Mixta calida DSM 22759	complete	GCA_002953215	665913.4	2791355150	665913	99.17
66792	ASM209535v1 assembly for Mixta calida LMG 25383	contig	GCA_002095355	665913.3	2791355158	665913

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
16499	Pantoea calida strain 1400/07 16S ribosomal RNA gene, partial sequence	GQ367478	1495	665913
124043	Pantoea calida gene for 16S ribosomal RNA, partial sequence, strain: LMG 25383.	AB907785	1495	665913
124043	Mixta calida strain LMG 25383 16S ribosomal RNA gene, partial sequence.	PQ524591	549	665913

GC content

@ref	GC-content (mol%)	Method
16499	57.4	high performance liquid chromatography (HPLC)
29720	57.4

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Mixta calida DSM 22759
NCBI: GCA_002953215

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	94.40	no
125439	motility	BacteriaNetⓘ	yes	70.60	no
125439	gram_stain	BacteriaNetⓘ	negative	98.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	98.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mixta calida DSM 22759
NCBI: GCA_002953215.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	yes
125438	anaerobic	anaerobicⓘ	no	97.70	yes
125438	aerobic	aerobicⓘ	no	66.96	yes
125438	spore-forming	spore-formingⓘ	no	88.64	no
125438	thermophilic	thermophileⓘ	no	99.50	yes
125438	flagellated	motile2+ⓘ	yes	80.30	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Alkane degradation mechanism of Mixta calida HXX308 isolated from sediment of the Mariana Trench.	Yang Y, He X, Zhang Y, Zhang XH.	Front Microbiol	10.3389/fmicb.2025.1579612	2025
Genetics	Draft genome and description of Mixta mediterraneensis strain Marseille-Q2057^T sp.nov., a new bacterium isolated from human healthy skin.	Boxberger M, Antezack A, Magnien S, Cassir N, La Scola B.	New Microbes New Infect	10.1016/j.nmni.2021.100840	2021
Phylogeny	Molecular validation of clinical Pantoea isolates identified by MALDI-TOF.	Soutar CD, Stavrinides J.	PLoS One	10.1371/journal.pone.0224731	2019
Genetics	Complete and Assembled Genome Sequences of Pantoea calida DSM 22759(T) and Pantoea gaviniae DSM 22758(T).	Stevens MJA, Zurfluh K, Stephan R	Genome Announc	10.1128/genomeA.00157-18	2018
	Erwinia plantamica sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat (Triticum aestivum L.).	Egorshina A, Lukyantsev M, Golubev S, Boulygina E, Khilyas I, Muratova A.	Microorganisms	10.3390/microorganisms13030474	2025
Phylogeny	Mixta tenebrionis sp. nov., isolated from the gut of the plastic-eating mealworm Tenebrio molitor L.	Xia M, Wang J, Huo YX, Yang Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.003826	2020
Phylogeny	Pantoea intestinalis sp. nov., isolated from the human gut.	Prakash O, Nimonkar Y, Vaishampayan A, Mishra M, Kumbhare S, Josef N, Shouche YS	Int J Syst Evol Microbiol	10.1099/ijsem.0.000419	2015
Phylogeny	Pantoea gaviniae sp. nov. and Pantoea calida sp. nov., isolated from infant formula and an infant formula production environment.	Popp A, Cleenwerck I, Iversen C, De Vos P, Stephan R	Int J Syst Evol Microbiol	10.1099/ijs.0.019430-0	2010

References

#16499	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22759
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26103	IJSEM 2786 2010 ( DOI 10.1099/ijs.0.019430-0 , PubMed 20061487 )
#29720	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26103
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68368	Automatically annotated from API 20E .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Mixta calida (DSM 22759, LMG 25383, 1400/07)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002953215

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media