Name: Kluyvera ascorbata 0648-74
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4973

Type strain:

Species: Kluyvera ascorbata

Strain Designation: 0648-74

Culture col. no.: DSM 4611, ATCC 33433, CDC 0648-74, CIP 82.95, GIFU 10603, IAM 14203, NBRC 102466, CCUG 15716, CECT 861, JCM 21070, LMG 7871

Strain history: CIP <- 1982, ATCC <- CDC: strain 0648-74

NCBI tax ID(s): 1006000 (strain), 51288 (species)

SI-ID 3183

LMG 7871, ATCC 33433, CCUG 15716, CECT 861, DSM 4611, IAM 14203, CIP 82.95, CCRC 14812, BCRC 14812, JCM 21070, CNCTC 5001, NBRC 102466, VTT E-981078

Other strains from Kluyvera ascorbata

K. ascorbata 2366, No.6(ß), CCUG 21164, DSM ...
K. ascorbata CCUG 21165
K. ascorbata CCUG 21166
K. ascorbata CCUG 21167
K. ascorbata CCUG 21209
K. ascorbata CCUG 21210
K. ascorbata CCUG 26138
K. ascorbata CCUG 26142
K. ascorbata CCUG 29960
K. ascorbata JCM 1681, CDC 0762-75

Section

Kluyvera ascorbata 0648-74 is an aerobe, mesophilic bacterium that was isolated from human sputum.

aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Kluyvera*
	Species	**Kluyvera ascorbata**
	Full Scientific Name (LPSN)	Kluyvera ascorbata Farmer et al. 1981

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1801]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #1801]

Culture medium growth

[Ref.: #1801]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1801]

Culture medium composition

expand / minimize

[Ref.: #1801]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #1801]

Culture medium growth

[Ref.: #1801]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1801]

Culture medium composition

expand / minimize

[Ref.: #37877]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #37877]

Culture medium growth

[Ref.: #37877]

Culture medium composition

expand / minimize

[Ref.: #116523]

Culture medium

CIP Medium 3

[Ref.: #116523]

Culture medium growth

[Ref.: #116523]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #1801]	growth	30 °C
[Ref.: #37877]	growth	30 °C
[Ref.: #46329]	growth	30 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #1801]	Temperature range	mesophilic
[Ref.: #37877]	Temperature range	mesophilic
[Ref.: #46329]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #46329]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	yes	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #1801]	17773	+/-	-	-	+	+	-	-	-	+	-	-	+	+	-	-	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1801]	Sample type/isolated from	human sputum

[Ref.: #67770]	Sample type/isolated from	Human sputum
[Ref.: #67770]	Geographic location (country and/or sea, region)	NC
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #116523]	Sample type/isolated from	Sputum
[Ref.: #116523]	Geographic location (country and/or sea, region)	North Carolina
[Ref.: #116523]	Country	United States of America
[Ref.: #116523]	Country ISO 3 Code	USA
[Ref.: #116523]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Fluids	#Sputum

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF310219 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	508
Soil Samples	165
Animal Samples	5631
Plant Samples	504
Total Samples	6808

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1801]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #116523]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1801]	Kluyvera ascorbata strain NBRC 102466 16S ribosomal RNA, partial sequence	NR_114107	1465	GenBank	51288 tax ID
[Ref.: #20218]	Kluyvera ascorbata ATCC 33433 16S ribosomal RNA gene, partial sequence	AF008579	1459	ENA	1006000 tax ID	*
[Ref.: #20218]	Kluyvera ascorbata 16S ribosomal RNA gene, partial sequence	AF310219	1437	ENA	1006000 tax ID	*
[Ref.: #20218]	Kluyvera ascorbata gene for 16S rRNA, partial sequence, strain: NBRC 102466	AB681808	1465	ENA	51288 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Kluyvera ascorbata ATCC 33433	GCA_000735365	contig	GenBank	1006000 tax ID
[Ref.: #66792]	Kluyvera ascorbata ATCC 33433	1006000.3	wgs	PATRIC	1006000 tax ID	*
[Ref.: #66792]	Kluyvera ascorbata ATCC 33433	2588253795	draft	JGI IMG/M	1006000 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Kluyvera ascorbata ATCC 33433 PATRIC: 1006000.3
[Ref.: #69480]	motile	yes	86.427	no
[Ref.: #69480]	flagellated	no	54.266	no
[Ref.: #69480]	gram-positive	no	98.148	no
[Ref.: #69480]	anaerobic	no	96.173	yes
[Ref.: #69480]	aerobic	yes	86.367	yes
[Ref.: #69480]	halophile	no	91.764	no
[Ref.: #69480]	spore-forming	no	91.488	no
[Ref.: #69480]	thermophile	no	99.291	yes
[Ref.: #69480]	glucose-util	yes	94.156	no
[Ref.: #69480]	glucose-ferment	yes	90.505	no

Availability in culture collections External links

[Ref.: #1801]

Culture collection no.

DSM 4611, ATCC 33433, CDC 0648-74, CIP 82.95, GIFU 10603, IAM 14203, NBRC 102466, CCUG 15716, CECT 861, JCM 21070, LMG 7871

[Ref.: #74469]

SI-ID 3183

References

#1801

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4611

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37877 ; Curators of the CIP;
#46329 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 15716

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#74469

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3183.1 )

#116523 Collection of Institut Pasteur ; Curators of the CIP; CIP 82.95
* These data were automatically processed and therefore are not curated

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Kluyvera ascorbata ATCC 33433

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