Name: Marinobacter santoriniensis NKSG1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 491

Type strain:

Species: Marinobacter santoriniensis

Strain Designation: NKSG1

Culture col. no.: DSM 21262

Strain history: <- K. M. Handley, SEAES, Univ. Manchester, United Kingdom; NKSG1

NCBI tax ID(s): 523742 (species)

SI-ID 403638

DSM 21262

Section

Marinobacter santoriniensis NKSG1 is a facultative anaerobe, moderately halophilic, mesophilic bacterium that was isolated from shallow marine hydrothermal sediment.

Gram-negative
motile
rod-shaped
facultative anaerobe
mesophilic
moderately halophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Alteromonadales*
	Family	*Alteromonadaceae*
	Genus	*Marinobacter*
	Species	**Marinobacter santoriniensis**
	Full Scientific Name (LPSN)	Marinobacter santoriniensis Handley et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #23124]	Gram stain	negative

[Ref.: #23124]	Cell length	1.2-4.5 µm

[Ref.: #23124]	Cell width	0.5-1.3 µm

[Ref.: #23124]	Cell shape	rod-shaped

[Ref.: #23124]	Motility	yes

[Ref.: #23124]	Flagellum arrangement	polar

[Ref.: #23124]

Colony size

7 mm

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15597]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #15597]

Culture medium growth

[Ref.: #15597]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15597]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #15597]			growth	37 °C
[Ref.: #23124]			optimum	35-40 °C

[Ref.: #15597]

Temperature range

mesophilic

		pH
[Ref.: #23124]	maximum	9.0
[Ref.: #23124]	minimum	5.5
[Ref.: #23124]	optimum	7.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23124]

Oxygen tolerance

facultative anaerobe

[Ref.: #23124]

Ability of spore formation

[Ref.: #23124]

Halophily / tolerance level

moderately halophilic

	Salt		Salt conc.
[Ref.: #23124]	NaCl	growth	>0.5	%
[Ref.: #23124]	NaCl	growth	<16	%
[Ref.: #23124]	NaCl	optimum	5-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23124]	acetate ChEBI	+	oxidation
[Ref.: #23124]	adipate ChEBI	+	carbon source
[Ref.: #23124]	butyrate ChEBI	+	oxidation
[Ref.: #23124]	citrate ChEBI	-	carbon source
[Ref.: #23124]	D-glucose ChEBI	-	carbon source
[Ref.: #23124]	D-mannitol ChEBI	-	carbon source
[Ref.: #23124]	D-mannose ChEBI	-	carbon source
[Ref.: #23124]	decanoate ChEBI	-	carbon source
[Ref.: #23124]	ferrous sulfate ChEBI	+	oxidation
[Ref.: #23124]	formate ChEBI	+	oxidation
[Ref.: #23124]	fumarate ChEBI	+	oxidation
[Ref.: #23124]	gluconate ChEBI	+	carbon source
[Ref.: #23124]	L-arabinose ChEBI	-	carbon source
[Ref.: #23124]	lactate ChEBI	+	oxidation
[Ref.: #23124]	malate ChEBI	+	carbon source
[Ref.: #23124]	malate ChEBI	+	oxidation
[Ref.: #23124]	maltose ChEBI	-	carbon source
[Ref.: #23124]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #23124]	nitrate ChEBI	+	reduction
[Ref.: #23124]	oxaloacetate ChEBI	+	oxidation
[Ref.: #23124]	phenylacetate ChEBI	+	carbon source
[Ref.: #23124]	propionate ChEBI	+	oxidation
[Ref.: #23124]	pyruvate ChEBI	+	oxidation
[Ref.: #23124]	succinate ChEBI	+	oxidation
[Ref.: #23124]	yeast extract	+	oxidation
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #23124]	6-phospho-beta-galactosidase	-	3.2.1.85
[Ref.: #23124]	acid phosphatase	+	3.1.3.2
[Ref.: #23124]	alkaline phosphatase	+	3.1.3.1
[Ref.: #23124]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #23124]	alpha-fucosidase	-	3.2.1.51
[Ref.: #23124]	alpha-galactosidase	-	3.2.1.22
[Ref.: #23124]	alpha-glucosidase	-	3.2.1.20
[Ref.: #23124]	alpha-mannosidase	-	3.2.1.24
[Ref.: #23124]	beta-glucosidase	-	3.2.1.21
[Ref.: #23124]	beta-glucuronidase	-	3.2.1.31
[Ref.: #23124]	cystine arylamidase	+	3.4.11.3
[Ref.: #23124]	esterase (C 4)	+
[Ref.: #23124]	esterase lipase (C 8)	+
[Ref.: #23124]	leucine arylamidase	+	3.4.11.1
[Ref.: #23124]	lipase (C 14)	+
[Ref.: #23124]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #23124]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #23124]	trypsin	-	3.4.21.4
[Ref.: #23124]	urease	+	3.5.1.5
[Ref.: #23124]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15597]	Sample type/isolated from	shallow marine hydrothermal sediment
[Ref.: #15597]	Geographic location (country and/or sea, region)	Santorini, Nea Kameni
[Ref.: #15597]	Country	Greece
[Ref.: #15597]	Country ISO 3 Code	GRC
[Ref.: #15597]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU496088 (>99% sequence identity) for Marinobacter from Microbeatlas

Aquatic Samples	499
Soil Samples	33
Animal Samples	18
Plant Samples	12
Total Samples	562

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15597]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15597]	Marinobacter santoriniensis strain NKSG1 16S ribosomal RNA gene, partial sequence	EU496088	1383	ENA	1288826 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Marinobacter santoriniensis NKSG1	GCA_000347775	contig	GenBank	1288826 tax ID	*
[Ref.: #66792]	Marinobacter santoriniensis NKSG1	1288826.3	wgs	PATRIC	1288826 tax ID	*
[Ref.: #66792]	Marinobacter santoriniensis NKSG1	2545824694	draft	JGI IMG/M	1288826 tax ID	*

[Ref.: #15597]

GC-content

58.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Marinobacter santoriniensis NKSG1 PATRIC: 1288826.3
[Ref.: #69480]	motile	yes	87.259	yes
[Ref.: #69480]	gram-positive	no	99.098	no
[Ref.: #69480]	anaerobic	no	97.078	yes
[Ref.: #69480]	aerobic	yes	62.197	yes
[Ref.: #69480]	halophile	yes	91.188	yes
[Ref.: #69480]	spore-forming	no	95.953	yes
[Ref.: #69480]	glucose-util	no	61.795	yes
[Ref.: #69480]	flagellated	yes	83.29	no
[Ref.: #69480]	glucose-ferment	no	89.542	no
[Ref.: #69480]	thermophile	no	99.274	yes

Availability in culture collections External links

[Ref.: #15597]

Culture collection no.

DSM 21262

[Ref.: #70170]

SI-ID 403638

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Marinobacter fonticola sp. nov., isolated from deep sea cold seep sediment.	Sun QL, Sun YL, Sun YY, Luan ZD, Lian C	Int J Syst Evol Microbiol	10.1099/ijsem.0.003895	2020	*
Phylogeny	Marinobacter bohaiensis sp. nov., a moderate halophile isolated from benthic sediment of the Bohai Sea.	Xu S, Wang D, Wei Y, Cui Q, Li W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003025	2018	*
Genetics	Genome Sequence of Hydrothermal Arsenic-Respiring Bacterium Marinobacter santoriniensis NKSG1T.	Handley KM, Upton M, Beatson SA, Hery M, Lloyd JR	Genome Announc	10.1128/genomeA.00231-13	2013	*
Phylogeny	Marinobacter oulmenensis sp. nov., a moderately halophilic bacterium isolated from brine of a salt concentrator.	Kharroub K, Aguilera M, Jimenez-Pranteda ML, Gonzalez-Paredes A, Ramos-Cormenzana A, Monteoliva-Sanchez M	Int J Syst Evol Microbiol	10.1099/ijs.0.027284-0	2010	*
Phylogeny	Marinobacter santoriniensis sp. nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment.	Handley KM, Hery M, Lloyd JR	Int J Syst Evol Microbiol	10.1099/ijs.0.003145-0	2009	*
Metabolism	Redox cycling of arsenic by the hydrothermal marine bacterium Marinobacter santoriniensis.	Handley KM, Hery M, Lloyd JR	Environ Microbiol	10.1111/j.1462-2920.2009.01890.x	2009	*

References

#15597

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21262

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23124

Kim M. Handley, Marina Héry, Jonathan R. Lloyd: Marinobacter santoriniensis sp. nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment. IJSEM 59: 886 - 892 2009 ( DOI 10.1099/ijs.0.003145-0 , PubMed 19329625 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#70170

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403638.1 )

* These data were automatically processed and therefore are not curated

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