Name: Citrobacter braakii M8B
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4339

Type strain:

Species: Citrobacter braakii

Strain Designation: M8B, M8BK

Culture col. no.: DSM 30040, ATCC 6750, CIP 110053, NCIB 8173, WDCM 00078, NCIMB 8173, CECT 8107

Strain history: CIP <- 2009, DSMZ <- ATCC <- C.H. Werkman : strain M8BK (Citrobacter intermedium) <- S.A. Koser, strain: M8B

NCBI tax ID(s): 57706 (species)

SI-ID 44358

ATCC 6750, DSM 30040, NCIB 8173, NCIMB 8173, KCTC 2006, CIP 110053, CECT 8107

Other strains from Citrobacter braakii

C. braakii 167, 80-58, DSM 17596, ATCC 51113, ... (type strain)
C. braakii 1249-78, CIP 105010
C. braakii 2990-58, CIP 104927
C. braakii CIP 53.62, NCTC 6021
C. braakii 3158-63, CIP 104924
C. braakii CCUG 489 G
C. braakii CCUG 3819
C. braakii CCUG 5011
C. braakii CCUG 5016
C. braakii CCUG 6228
C. braakii CCUG 32589
C. braakii CCUG 33100
C. braakii CCUG 34426
C. braakii CCUG 41766
C. braakii CCUG 41989
C. braakii CCUG 43795
C. braakii CIMB 47, CIMB 04-3848, CRBIP17.47

Section

Citrobacter braakii M8B is a facultative anaerobe, mesophilic, motile bacterium that was isolated from faeces.

motile
facultative anaerobe
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Citrobacter*
	Species	**Citrobacter braakii**
	Full Scientific Name (LPSN)	Citrobacter braakii Brenner et al. 1993

Information on morphological and physiological properties Morphology

[Ref.: #116985]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.99

[Ref.: #116985]	Cell shape	rod-shaped

[Ref.: #116985]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %97.133

Information on culture and growth conditions Culture and growth conditions

[Ref.: #9098]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #9098]

Culture medium growth

[Ref.: #9098]

Culture medium link

Medium recipe at MediaDive

[Ref.: #9098]

Culture medium composition

expand / minimize

[Ref.: #41803]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #41803]

Culture medium growth

[Ref.: #41803]

Culture medium composition

expand / minimize

[Ref.: #116985]

Culture medium

CIP Medium 328

[Ref.: #116985]

Culture medium growth

[Ref.: #116985]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #116985]

Culture medium

CIP Medium 3

[Ref.: #116985]

Culture medium growth

[Ref.: #116985]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #9098]			growth	30 °C
[Ref.: #41803]			growth	30 °C

[Ref.: #9098]	Temperature range	mesophilic
[Ref.: #41803]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116985]

Oxygen tolerance

facultative anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.956

[Ref.: #9098]

Name of produced compound

trimethylene glycol

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #116985]	nitrate ChEBI	+	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #116985]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	yes	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API 20E
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116985]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #116985]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #116985]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #9098]	16810	+	+	-	-	+	+	-	-	-	-	-	+	+	-	+	+	-	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116985]	12327	-	+	-	-	-	+	-	-	-	-	+	+	-	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #9098]	Sample type/isolated from	faeces

[Ref.: #116985]	Sample type/isolated from	Faeces
* marker position based on {}

Isolation sources categories

#Host Body Product

#Gastrointestinal tract

#Feces (Stool)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #9098]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #116985]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Citrobacter braakii strain FDAARGOS 1422	57706.143	complete	PATRIC	57706 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Citrobacter braakii strain FDAARGOS 1422 strain Not applicable PATRIC: 57706.143
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Citrobacter braakii strain FDAARGOS 1422 strain Not applicable PATRIC: 57706.143
[Ref.: #69480]	gram-positive	no	98.153	no
[Ref.: #69480]	anaerobic	no	95.214	no
[Ref.: #69480]	halophile	no	91.347	no
[Ref.: #69480]	spore-forming	no	92.834	no
[Ref.: #69480]	glucose-util	yes	94.608	no
[Ref.: #69480]	thermophile	no	99.086	yes
[Ref.: #69480]	motile	yes	90.61	no
[Ref.: #69480]	flagellated	no	50	no
[Ref.: #69480]	aerobic	yes	80.61	no
[Ref.: #69480]	glucose-ferment	yes	95.028	no

Availability in culture collections External links

[Ref.: #9098]

Culture collection no.

DSM 30040, ATCC 6750, CIP 110053, NCIB 8173, WDCM 00078, NCIMB 8173, CECT 8107

[Ref.: #73831]

SI-ID 44358

References

#9098

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 30040

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41803 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#73831

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID44358.1 )

#116985 Collection of Institut Pasteur ; Curators of the CIP; CIP 110053
* These data were automatically processed and therefore are not curated

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Citrobacter braakii strain FDAARGOS 1422 strain Not applicable

Citrobacter braakii strain FDAARGOS 1422 strain Not applicable

Change proposal

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