Citrobacter koseri (DSM 4570, ATCC 27156, CIP 82.94)

Name: Citrobacter koseri (DSM 4570, ATCC 27156, CIP 82.94)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 4328

Type strain

Strain Designation 3613-63

Culture col. no. DSM 4570 ATCC 27156 CIP 82.94 CDC 3613-63 NCTC 10849 9 more

NCBI tax ID(s) 545

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- CIP; CIP 82.94 <- ATCC; ATCC 27156 <- W. H. Ewing, CDC, Atlanta; CDC 3613-63 (Citrobacter diversus) JCM <-- R. Sakazaki <-- ATCC 27156 <-- W. H. Ewing CDC 3613-63. CIP <- 1982, ATCC <- W.H. Ewing, CDC: strain 3613-63

Links

Citrobacter koseri 3613-63 is a facultative anaerobe, Gram-negative, motile bacterium of the family Enterobacteriaceae.

Gram-negative motile rod-shaped facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 311037

LMG 5741,ATCC 27156,CCUG 14559,CCUG 14799,JCM 1659,NCTC 10849,CCUG 5115,CECT 856,DSM 4570,CCRC 14803,CIP 82.94,NCCB 92009,LMD 92.9,BCRC 14803,CDBB 957,CNCTC 5081

@ref 20215

Last LPSN update 2026-05-29 08:25:54

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Enterobacterales

Family Enterobacteriaceae

Genus Citrobacter

Species Citrobacter koseri

Full scientific name Citrobacter koseri Frederiksen 1970 (Approved Lists 1980)

Synonyms (4)

Levinea malonatica
Citrobacter diversus
"Aerobacter diversum"
Citrobacter diversum

BacDive ID	Other strains from **Citrobacter koseri** (33)	Type strain
4329	C. koseri DSM 4595, ATCC 27028, CCM 2537, CIP 82.87, ... (type strain)
4330	C. koseri DSM 4596, ATCC 25408, CIP 82.88, NCTC 10810, ...
4331	C. koseri 9910, DSM 46297, ATCC 27027, CCM 2536, IMET ...
134844	C. koseri CIP 76.3
135384	C. koseri CIP 72.14
135668	C. koseri CIP 72.8
136758	C. koseri 1381-70, CIP 104933
137327	C. koseri CIP 106949
138183	C. koseri 32, CIP 105177, ATCC 29936
138414	C. koseri Cd4, CIP 105542
138950	C. koseri Riehli, CIP 72.11
139155	C. koseri 8127-86, CIP 104558
141202	C. koseri CCUG 177
141653	C. koseri CCUG 4427
141711	C. koseri CCUG 5071
141733	C. koseri CCUG 5847 A
141739	C. koseri CCUG 5885
141751	C. koseri CCUG 6266
141773	C. koseri CCUG 6597
141774	C. koseri CCUG 6639
141775	C. koseri CCUG 6640
141785	C. koseri CCUG 6741
143101	C. koseri CCUG 17211
143923	C. koseri CCUG 21861
144517	C. koseri CCUG 25452
144818	C. koseri CCUG 26653
145886	C. koseri CCUG 29880
145887	C. koseri CCUG 29881
147561	C. koseri CCUG 33803
148610	C. koseri CCUG 35708
151611	C. koseri CCUG 44448
154860	C. koseri CCUG 55438
154991	C. koseri CCUG 55990

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
120495	negative	rod-shaped
125438	negative			99.5

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
1776	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
37361	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
1776	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
1776	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
120495	CIP Medium 72	Medium recipe at CIP
120495	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
1776	positive	growth	30
37361	positive	growth	30
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

120495

Oxygen tolerancefacultative anaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68368	27613 ChEBI	amygdalin	+	fermentation	from API 20E
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68371	18333 ChEBI	D-arabitol	+	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68368	17634 ChEBI	D-glucose	+	fermentation	from API 20E
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68368	16899 ChEBI	D-mannitol	+	fermentation	from API 20E
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	+	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	+	builds acid from	from API 50CH acid
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68368	30849 ChEBI	L-arabinose	+	fermentation	from API 20E
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	+	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	+	builds acid from	from API 50CH acid
68368	62345 ChEBI	L-rhamnose	+	fermentation	from API 20E
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68368	28053 ChEBI	melibiose	-	fermentation	from API 20E
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	+	builds acid from	from API 50CH acid
68368	17268 ChEBI	myo-inositol	+	fermentation	from API 20E
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
120495	17632 ChEBI	nitrate	+	reduction
120495	16301 ChEBI	nitrite	+	reduction
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
68371	0 ChEBI	Potassium 2-ketogluconate	+	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	+	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	+	builds acid from	from API 50CH acid
68368	30911 ChEBI	sorbitol	+	fermentation	from API 20E
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68368	27897 ChEBI	tryptophan	+	energy source	from API 20E
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
120495	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		+	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120495	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68368	cytochrome oxidase	-	1.9.3.1	from API 20E
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68368	gelatinase	-		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
120495	oxidase	-
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
120495	urease	+	3.5.1.5
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
1776	+	+	-	+	+	-	-	-	+	-	-	+	+	+	+	+	-	-	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120495	-	+	-	-	-	+	-	-	+	-	+	+	-	+	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
120495	not determinedn.d.	+	-	+/-	+	+	+	-	+	-	+	+	+	+	-	+	+	+	+	+	-	+	+	-	+/-	-	+/-	+	+	+	-	-	+	-	-	-	-	-	-	+	-	+/-	-	-	+	+	-	+	+	+

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1776	2	Risk group (German classification) According to TRBA 466
120495	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
67770	28265_B01 assembly for Citrobacter koseri NCTC10849	contig	GCA_900706735	545.113	545	45.76

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
67770	Citrobacter koseri gene for 16S rRNA, partial sequence, strain: NBRC 105690	AB682264	1467	545
67770	Citrobacter diversus strain CDC 3613-63 16S ribosomal RNA gene, partial sequence	AF025372	1512	545
67770	Citrobacter koseri gene for 16S ribosomal RNA, partial sequence, strain: JCM 1659	LC060909	1464	545
124043	Citrobacter koseri gene for 16S ribosomal RNA, partial sequence, strain: NBRC 105690.	AB741694	1324	545

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: 28265_B01 assembly for Citrobacter koseri NCTC10849
BV-BRC: 545.113

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	82.61	no
125439	gram_stain	BacteriaNetⓘ	negative	81.47	no
125439	motility	BacteriaNetⓘ	yes	69.78	no
125439	spore_formation	BacteriaNetⓘ	no	88.22	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Citrobacter koseri strain NCTC10849
PATRIC: 545.113

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	no
125438	anaerobic	anaerobicⓘ	no	96.47	yes
125438	spore-forming	spore-formingⓘ	no	87.14	no
125438	aerobic	aerobicⓘ	no	71.18	yes
125438	thermophilic	thermophileⓘ	no	99.00	yes
125438	flagellated	motile2+ⓘ	yes	78.37	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry as a reliable proteomic method for characterization of Escherichia coli and Salmonella isolates.	Shell WS, Sayed ML, Allah FMG, Gamal FEM, Khedr AA, Samy AA, Ali AHM.	Vet World	10.14202/vetworld.2017.1083-1093	2017
Enzymology	Molecular method for detection of total coliforms in drinking water samples.	Maheux AF, Boudreau DK, Bisson MA, Dion-Dupont V, Bouchard S, Nkuranga M, Bergeron MG, Rodriguez MJ.	Appl Environ Microbiol	10.1128/aem.00546-14	2014
Phylogeny	Evaluation of a rapid direct assay for identification of bacteria and the mec A and van genes from positive-testing blood cultures.	Eigner U, Weizenegger M, Fahr AM, Witte W.	J Clin Microbiol	10.1128/jcm.43.10.5256-5262.2005	2005
Metabolism	Characterization of the Salmonella enterica serovar Typhimurium ydcI gene, which encodes a conserved DNA binding protein required for full acid stress resistance.	Jennings ME, Quick LN, Soni A, Davis RR, Crosby K, Ott CM, Nickerson CA, Wilson JW.	J Bacteriol	10.1128/jb.01335-10	2011
	AOAC-OMA/MicroVal Harmonized Validation of Peel PlateTM EB (Enterobacteriaceae Bacteria), First Action 2018.05.	Salter RS, Durbin GW, Martinez D, Bird P, Bastin B, Crowley E.	J AOAC Int	10.1093/jaoacint/qsaa067	2020
Enzymology	Rapid concentration and molecular enrichment approach for sensitive detection of Escherichia coli and Shigella species in potable water samples.	Maheux AF, Bissonnette L, Boissinot M, Bernier JL, Huppe V, Picard FJ, Berube E, Bergeron MG.	Appl Environ Microbiol	10.1128/aem.02337-10	2011
	Citrobacter rodentium, the causative agent of transmissible murine colonic hyperplasia, exhibits clonality: synonymy of C. rodentium and mouse-pathogenic Escherichia coli.	Luperchio SA, Newman JV, Dangler CA, Schrenzel MD, Brenner DJ, Steigerwalt AG, Schauer DB.	J Clin Microbiol	10.1128/jcm.38.12.4343-4350.2000	2000
Enzymology	Novel class A beta-lactamase Sed-1 from Citrobacter sedlakii: genetic diversity of beta-lactamases within the Citrobacter genus.	Petrella S, Clermont D, Casin I, Jarlier V, Sougakoff W.	Antimicrob Agents Chemother	10.1128/aac.45.8.2287-2298.2001	2001
Metabolism	Anaerobic malonate decarboxylation by Citrobacter diversus. Growth and metabolic studies, and evidence of ATP formation.	Janssen PH, Harfoot CG	Arch Microbiol	10.1007/BF00249107	1992

References

#1776	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4570
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#37361	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#120495	Collection of Institut Pasteur ; Curators of the CIP; CIP 82.94
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Citrobacter koseri (DSM 4570, ATCC 27156, CIP 82.94)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

API 50CHac

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900706735

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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