Name: Staphylothermus hellenicus P8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4203

Type strain:

Species: Staphylothermus hellenicus

Strain Designation: P8

Culture col. no.: DSM 12710, JCM 10830

Strain history: M. Thomm P8.

NCBI tax ID(s): 591019 (strain), 84599 (species)

SI-ID 49346

DSM 12710, JCM 10830

Section

Staphylothermus hellenicus P8 is an anaerobe, spore-forming, hyperthermophilic archaeon that forms irregular colonies and was isolated from hydrothermal site off Palaeochori Bay.

coccus-shaped
colony-forming
spore-forming
anaerobe
hyperthermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Thermoproteota*
	Class	*Thermoprotei*
	Order	*Desulfurococcales*
	Family	*Desulfurococcaceae*
	Genus	*Staphylothermus*
	Species	**Staphylothermus hellenicus**
	Full Scientific Name (LPSN)	Staphylothermus hellenicus Arab et al. 2000

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.986

[Ref.: #23227]	Cell length	0.8-1.3 µm

[Ref.: #23227]	Cell shape	coccus-shaped

[Ref.: #23227]

Colony shape

irregular

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4794]

Culture medium

STETTERIA MEDIUM (DSMZ Medium 795)

[Ref.: #4794]

Culture medium growth

[Ref.: #4794]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4794]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #4794]	growth	85 °C
[Ref.: #23227]	growth	70-90 °C
[Ref.: #23227]	optimum	85 °C
[Ref.: #67770]	growth	85 °C

[Ref.: #4794]	Temperature range	hyperthermophilic
[Ref.: #23227]	Temperature range	hyperthermophilic
[Ref.: #67770]	Temperature range	hyperthermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %100

	pH	Kind of pH		pH
[Ref.: #23227]			optimum	6.0
[Ref.: #23227]			growth	4.5-7.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4794]	Oxygen tolerance	anaerobe
[Ref.: #23227]	Oxygen tolerance	obligate anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.561

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.94

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23227]		NaCl		growth	2-8	%
[Ref.: #23227]		NaCl		optimum	4	%

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23227]		yeast extract	+	carbon source

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4794]	Sample type/isolated from	hydrothermal site off Palaeochori Bay
[Ref.: #4794]	Geographic location (country and/or sea, region)	Aegean Sea, Milos
[Ref.: #4794]	Country	Greece
[Ref.: #4794]	Country ISO 3 Code	GRC
[Ref.: #4794]	Continent	Europe

[Ref.: #23227]	Sample type/isolated from	geothermally heated sediment at Palaeochori Bay, located at a depth of 9.4 m

[Ref.: #67770]	Sample type/isolated from	Geothermally heated sediment at Palaeochori Bay
[Ref.: #67770]	Geographic location (country and/or sea, region)	Milos
[Ref.: #67770]	Country	Greece
[Ref.: #67770]	Country ISO 3 Code	GRC
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ012645 (>99% sequence identity) for Staphylothermus hellenicus from Microbeatlas

Aquatic Samples	67
Soil Samples	1
Animal Samples
Plant Samples
Total Samples	68

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4794]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23227]	Staphylothermus achaiicus (isolate P8) 16S rRNA gene, partial	AJ012645	1372	GenBank	591019 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Staphylothermus hellenicus DSM 12710	GCA_000092465	complete	GenBank	591019 tax ID	*
[Ref.: #66792]	Staphylothermus hellenicus P8, DSM 12710	646564573	complete	JGI IMG/M	591019 tax ID	*
[Ref.: #66792]	Staphylothermus hellenicus DSM 12710	591019.7	complete	PATRIC	591019 tax ID	*

[Ref.: #4794]	GC-content	38 mol%	high performance liquid chromatography (HPLC)
[Ref.: #4794]	GC-content	36.8 mol%	sequence analysis
[Ref.: #67770]	GC-content	38.8 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Staphylothermus hellenicus DSM 12710 DSM 12710 NCBI: GCA_000092465.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Staphylothermus hellenicus DSM 12710 DSM 12710 NCBI: GCA_000092465.1
[Ref.: #69480]	motile	no	63.929	no
[Ref.: #69480]	flagellated	no	92.543	no
[Ref.: #69480]	gram-positive	no	94.69	no
[Ref.: #69480]	anaerobic	yes	98.675	yes
[Ref.: #69480]	aerobic	no	94.67	yes
[Ref.: #69480]	halophile	no	77.716	no
[Ref.: #69480]	spore-forming	no	98.284	no
[Ref.: #69480]	glucose-util	yes	86.286	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-ferment	no	53.858	no

Availability in culture collections External links

[Ref.: #4794]

Culture collection no.

DSM 12710, JCM 10830

[Ref.: #73703]

SI-ID 49346

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermococcus aegaeicus sp. nov. and Staphylothermus hellenicus sp. nov., two novel hyperthermophilic archaea isolated from geothermally heated vents off Palaeochori Bay, Milos, Greece.	Arab H, Volker H, Thomm M	Int J Syst Evol Microbiol	10.1099/00207713-50-6-2101	2000	*

References

#4794

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12710

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23227

H Arab, H Völker, M Thomm: Thermococcus aegaeicus sp. nov. and Staphylothermus hellenicus sp. nov., two novel hyperthermophilic archaea isolated from geothermally heated vents off Palaeochori Bay, Milos, Greece. IJSEM 50: 2101 - 2108 2000 ( DOI 10.1099/00207713-50-6-2101 , PubMed 11155985 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#73703

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49346.1 )

* These data were automatically processed and therefore are not curated

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Staphylothermus hellenicus DSM 12710 DSM 12710

Staphylothermus hellenicus DSM 12710 DSM 12710

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