Name: Gluconacetobacter sacchari DSM 12717
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 42

Type strain:

Species: Gluconacetobacter sacchari

Culture col. no.: DSM 12717, SRI 1794, CIP 106693

Strain history: CIP <- 2000, ACM <- 1995, I. Franke, Queensland Univ., Australia: strain SRI 1794

NCBI tax ID(s): 1307940 (strain), 92759 (species)

SI-ID 68639

CIP 106693, DSM 12717, LMG 19747, BCRC 17165, CCRC 17165, BCC 36448, TBRC 478

Other strains from Gluconacetobacter sacchari

G. sacchari CCUG 36906
G. sacchari CCUG 36907

Section

Gluconacetobacter sacchari DSM 12717 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from mealy bug from sugar cane.

Gram-negative
motile
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Gluconacetobacter*
	Species	**Gluconacetobacter sacchari**
	Full Scientific Name (LPSN)	Gluconacetobacter sacchari Franke et al. 1999

Information on morphological and physiological properties Morphology

[Ref.: #23243]	Gram stain	negative
[Ref.: #122330]	Gram stain	negative

[Ref.: #23243]	Cell length	1.3-2.2 µm

[Ref.: #23243]	Cell width	0.7-0.9 µm

[Ref.: #23243]	Cell shape	rod-shaped
[Ref.: #122330]	Cell shape	rod-shaped

[Ref.: #23243]	Motility	yes
[Ref.: #122330]	Motility	yes

[Ref.: #23243]	Flagellum arrangement	peritrichous

[Ref.: #23243]	Colony color	khaki-brown
[Ref.: #23243]	Incubation period	3-4 days
[Ref.: #23243]	Cultivation medium used	WL nutrient agar

[Ref.: #23243]	Colony color	brown
[Ref.: #23243]	Cultivation medium used	GYC

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4795]

Culture medium

SABOURAND GLUCOSE MEDIUM (DSMZ Medium 1429)

[Ref.: #4795]

Culture medium growth

[Ref.: #4795]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4795]

Culture medium composition

expand / minimize

[Ref.: #4795]

Culture medium

GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105)

[Ref.: #4795]

Culture medium growth

[Ref.: #4795]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4795]

Culture medium composition

expand / minimize

[Ref.: #23243]

Culture medium

GYC

[Ref.: #23243]

Culture medium growth

[Ref.: #23243]

Culture medium composition

expand / minimize

[Ref.: #23243]

Culture medium

WL nutrient agar

[Ref.: #23243]

Culture medium growth

[Ref.: #23243]

Culture medium composition

expand / minimize

[Ref.: #39554]

Culture medium

MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii

[Ref.: #39554]

Culture medium growth

[Ref.: #39554]

Culture medium composition

expand / minimize

[Ref.: #122330]

Culture medium

CIP Medium 314

[Ref.: #122330]

Culture medium growth

[Ref.: #122330]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #122330]

Culture medium

CIP Medium 1

[Ref.: #122330]

Culture medium growth

[Ref.: #122330]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4795]		growth	28 °C
[Ref.: #39554]		growth	30 °C
[Ref.: #122330]		growth	22-30 °C
[Ref.: #122330]	no	growth	10 °C
[Ref.: #122330]	no	growth	37 °C
[Ref.: #122330]	no	growth	41 °C
[Ref.: #122330]	no	growth	45 °C

[Ref.: #4795]	Temperature range	mesophilic
[Ref.: #39554]	Temperature range	mesophilic
[Ref.: #122330]	Temperature range	psychrophilic
[Ref.: #122330]	Temperature range	mesophilic
[Ref.: #122330]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23243]	Oxygen tolerance	obligate aerobe
[Ref.: #122330]	Oxygen tolerance	obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #122330]	NaCl		growth	0	%
[Ref.: #122330]	NaCl	no	growth	2	%
[Ref.: #122330]	NaCl	no	growth	4	%
[Ref.: #122330]	NaCl	no	growth	6	%
[Ref.: #122330]	NaCl	no	growth	8	%
[Ref.: #122330]	NaCl	no	growth	10	%

[Ref.: #23243]	Name of tolerated compound	Glucose
[Ref.: #23243]	Tolerance percentage	30
[Ref.: #23243]	Name of tolerated compound	Malachite green
[Ref.: #23243]	Tolerance percentage	0.01

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23243]	acetate ChEBI	+	carbon source
[Ref.: #23243]	acetate ChEBI	+	oxidation
[Ref.: #23243]	acetylene ChEBI	-	reduction
[Ref.: #23243]	D-mannitol ChEBI	-	builds acid from
[Ref.: #23243]	ethanol ChEBI	+	carbon source
[Ref.: #23243]	ethanol ChEBI	+	oxidation
[Ref.: #23243]	galactitol ChEBI	-	carbon source
[Ref.: #23243]	glucose ChEBI	+	builds acid from
[Ref.: #23243]	glutamate ChEBI	+	growth
[Ref.: #23243]	glycine ChEBI	-	carbon source
[Ref.: #122330]	hippurate ChEBI	-	hydrolysis
[Ref.: #23243]	L-asparagine ChEBI	-	carbon source
[Ref.: #23243]	L-glutamine ChEBI	-	carbon source
[Ref.: #23243]	L-threonine ChEBI	-	carbon source
[Ref.: #23243]	L-tryptophan ChEBI	-	carbon source
[Ref.: #23243]	lactate ChEBI	+	oxidation
[Ref.: #23243]	mannitol ChEBI	+	growth
[Ref.: #23243]	methanol ChEBI	-	carbon source
[Ref.: #23243]	nitrate ChEBI	-	reduction
[Ref.: #122330]	nitrate ChEBI	+	reduction
[Ref.: #122330]	nitrite ChEBI	-	reduction
[Ref.: #23243]	propanol ChEBI	+	builds acid from
[Ref.: #23243]	sorbitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23243]	2-dehydro-D-gluconate ChEBI	yes
[Ref.: #23243]	5-dehydro-D-gluconate ChEBI	yes
[Ref.: #23243]	hydrogen sulfide ChEBI	no
[Ref.: #122330]	indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122330]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122330]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122330]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #122330]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122330]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122330]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #122330]	1770	not determinedn.d.	-	+	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122330]	9725	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4795]	Sample type/isolated from	mealy bug from sugar cane
[Ref.: #4795]	Geographic location (country and/or sea, region)	Queensland
[Ref.: #4795]	Country	Australia
[Ref.: #4795]	Country ISO 3 Code	AUS
[Ref.: #4795]	Continent	Australia and Oceania

[Ref.: #23243]	Sample type/isolated from	leaf sheath of sugar cane and pink sugar-cane mealy bug (Saccharicoccus sacchari)
[Ref.: #23243]	Enrichment culture	WL nutrient agar
[Ref.: #23243]	Enrichment culture composition	add 100 mg/L cycloheximide
[Ref.: #23243]	Isolation/enrichment procedure	Serial dilutions plated

[Ref.: #122330]	Sample type/isolated from	Sugar cane
[Ref.: #122330]	Country	Australia
[Ref.: #122330]	Country ISO 3 Code	AUS
[Ref.: #122330]	Continent	Australia and Oceania
[Ref.: #122330]	Isolation date	1984
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host Body-Site	#Plant	#Leaf (Phyllosphere)
#Host	#Arthropoda	#Insecta
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JF794008 (>99% sequence identity) for Gluconacetobacter from Microbeatlas

Aquatic Samples	1
Soil Samples
Animal Samples	6
Plant Samples
Total Samples	7

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4795]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122330]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4795]	Gluconacetobacter sacchari isolate SRI1794 16S ribosomal RNA gene, partial sequence	AF127407	1427	ENA	92759 tax ID
[Ref.: #20218]	Gluconacetobacter sacchari strain DSM 12717 16S ribosomal RNA gene, partial sequence	JF794008	1364	ENA	92759 tax ID	*

[Ref.: #4795]

GC-content

65 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #4795]

Culture collection no.

DSM 12717, SRI 1794, CIP 106693

[Ref.: #69727]

SI-ID 68639

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug.	Franke IH, Fegan M, Hayward C, Leonard G, Stackebrandt E, Sly LI	Int J Syst Bacteriol	10.1099/00207713-49-4-1681	1999	*

References

#4795

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12717

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23243

Ingrid H. Franke, Mark Fegan, Chris Hayward, Graham Leonard, Erko Stackebrandt, Lindsay I. Sly: Description of Gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug. IJSEM 49: 1681 - 1693 1999 ( DOI 10.1099/00207713-49-4-1681 , PubMed 10555349 )

#39554 ; Curators of the CIP;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69727

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID68639.1 )

#122330 Collection of Institut Pasteur ; Curators of the CIP; CIP 106693
* These data were automatically processed and therefore are not curated

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