Name: Aeropyrum pernix K1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4195

Type strain:

Species: Aeropyrum pernix

Strain Designation: K1

Culture col. no.: DSM 11879, JCM 9820, ATCC 700893, NBRC 100138

Strain history: N. Nomura K1.

NCBI tax ID(s): 56636 (species)

SI-ID 44195

ATCC 700893, JCM 9820, DSM 11879, NBRC 100138

Section

Aeropyrum pernix K1 is an aerobe, heterotroph, hyperthermophilic archaeon that forms irregular colonies and was isolated from marine solfataric vent.

coccus-shaped
colony-forming
Gram-negative
motile
aerobe
heterotroph
hyperthermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Thermoproteota*
	Class	*Thermoprotei*
	Order	*Desulfurococcales*
	Family	*Desulfurococcaceae*
	Genus	*Aeropyrum*
	Species	**Aeropyrum pernix**
	Full Scientific Name (LPSN)	Aeropyrum pernix Sako et al. 1996

Information on morphological and physiological properties Morphology

[Ref.: #23222]	Gram stain	negative

[Ref.: #23222]	Cell length	0.8-1.0 µm

[Ref.: #23222]	Cell shape	coccus-shaped

[Ref.: #23222]	Motility	yes

[Ref.: #23222]	Colony color	brown
[Ref.: #23222]	Colony shape	irregular

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4521]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #4521]

Culture medium growth

[Ref.: #4521]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4521]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #4521]		growth	90 °C
[Ref.: #23222]		growth	70-100 °C
[Ref.: #23222]	no	growth	68 °C
[Ref.: #23222]	no	growth	102 °C
[Ref.: #23222]		optimum	90-95 °C
[Ref.: #67770]		growth	90 °C

[Ref.: #4521]	Temperature range	hyperthermophilic
[Ref.: #23222]	Temperature range	hyperthermophilic
[Ref.: #23222]	Temperature range	thermophilic
[Ref.: #23222]	Temperature range	hyperthermophilic
[Ref.: #67770]	Temperature range	hyperthermophilic

	pH	Kind of pH		pH
[Ref.: #23222]			optimum	7.0
[Ref.: #23222]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23222]

Oxygen tolerance

aerobe

[Ref.: #23222]

Nutrition type

heterotroph

[Ref.: #67770]

Observation

quinones: DMK-6(H_n), MTK-6(H_n)

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23222]		thiosulfate ChEBI	+	growth

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4521]	Sample type/isolated from	marine solfataric vent
[Ref.: #4521]	Geographic location (country and/or sea, region)	Kodakara-Jima Island
[Ref.: #4521]	Country	Japan
[Ref.: #4521]	Country ISO 3 Code	JPN
[Ref.: #4521]	Continent	Asia

[Ref.: #23222]	Sample type/isolated from	hot sedimentary materials and venting waters at a coastal solfataric vent

[Ref.: #67770]	Sample type/isolated from	Coastal solfataric vent at Kodakara-Jima Island
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Condition	#Sulfuric	-
#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Volcanic

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4521]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4521]	Aeropyrum pernix gene for 16S rRNA, partial sequence	D83259	1444	ENA	56636 tax ID
[Ref.: #67770]	Aeropyrum pernix genes for 16S rRNA, 23S rRNA and rRNA intron-encoded homing endonuclease, complete cds	AB008745	10777	ENA	56636 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Aeropyrum pernix K1	GCA_000011125	complete	GenBank	272557 tax ID	*
[Ref.: #66792]	Aeropyrum pernix K1	2923402010	complete	JGI IMG/M	272557 tax ID	*
[Ref.: #66792]	Aeropyrum pernix K1	272557.25	complete	PATRIC	272557 tax ID	*

[Ref.: #4521]	GC-content	67 mol%	high performance liquid chromatography (HPLC)
[Ref.: #23222]	GC-content	67.0 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Aeropyrum pernix K1 K1 NCBI: GCA_000011125.1
[Ref.: #69480]	motile	no	57.388	yes
[Ref.: #69480]	flagellated	no	93.948	no
[Ref.: #69480]	gram-positive	no	96.458	yes
[Ref.: #69480]	anaerobic	yes	80.312	yes
[Ref.: #69480]	halophile	no	61.253	no
[Ref.: #69480]	spore-forming	no	96.572	no
[Ref.: #69480]	thermophile	yes	99.999	no
[Ref.: #69480]	glucose-util	yes	79.971	no
[Ref.: #69480]	aerobic	no	70.343	no
[Ref.: #69480]	glucose-ferment	no	78.05	no

Availability in culture collections External links

[Ref.: #4521]

Culture collection no.

DSM 11879, JCM 9820, ATCC 700893, NBRC 100138

[Ref.: #73695]

SI-ID 44195

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1.	Han JS, Kosugi Y, Ishida H, Ishikawa K	Eur J Biochem	10.1046/j.0014-2956.2001.02731.x	2002	*
Enzymology	Glutamate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix K1: enzymatic characterization, identification of the encoding gene, and phylogenetic implications.	Bhuiya MW, Sakuraba H, Kujo C, Nunoura-Kominato N, Kawarabayasi Y, Kikuchi H, Ohshima T	Extremophiles	10.1007/s007920070002	2000	*
Enzymology	Purification and characterization of an intracellular heat-stable proteinase (pernilase) from the marine hyperthermophilic archaeon Aeropyrum pernix K1.	Chavez Croocker P, Sako Y, Uchida A	Extremophiles	10.1007/s007920050093	1999	*
Enzymology	An extremely heat-stable extracellular proteinase (aeropyrolysin) from the hyperthermophilic archaeon Aeropyrum pernix K1.	Sako Y, Croocker PC, Ishida Y	FEBS Lett	10.1016/s0014-5793(97)01153-8	1997	*
Phylogeny	Aeropyrum pernix gen. nov., sp. nov., a novel aerobic hyperthermophilic archaeon growing at temperatures up to 100 degrees C.	Sako Y, Nomura N, Uchida A, Ishida Y, Morii H, Koga Y, Hoaki T, Maruyama T	Int J Syst Bacteriol	10.1099/00207713-46-4-1070	1996	*

References

#4521

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11879

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23222

YOSHIHIKO SAKO, NORIMICHI NOMURA, ARITSUNE UCHIDA, YUZABURO ISHIDA, HIROYUKI MORII, YOSUKE KOGA, TOSHIHIRO HOAKI, TADASHI MARUYAMA: Aeropyrum pernix gen. nov., sp. nov., a Novel Aerobic Hyperthermophilic Archaeon Growing at Temperatures up to 100°C. IJSEM 46: 1070 - 1077 1996 ( DOI 10.1099/00207713-46-4-1070 , PubMed 8863437 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#73695

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID44195.1 )

* These data were automatically processed and therefore are not curated

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Aeropyrum pernix K1 K1

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