Alicyclobacillus acidocaldarius subsp. acidocaldarius (CCUG 28521, NRRL B-14509, DSM 446)

Name: Alicyclobacillus acidocaldarius subsp. acidocaldarius (CCUG 28521, NRRL B-14509, DSM 446)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 416

Type strain

Strain Designation 104-IA, 104-1A

Culture col. no. CCUG 28521 NRRL B-14509 DSM 446 ATCC 27009 CIP 106131 11 more

NCBI tax ID(s) 521098 405212 1388

Special Collections DSMZ CCUG JCM KCTC CIP

History <- ATCC <- T.D. Brock, 104-IA DSM 446 <-- ATCC 27009 <-- T. D. Brock 104-IA. CIP <- 1999, ATCC <- T.D. Brock: strain 104-1A

Links

Alicyclobacillus acidocaldarius subsp. acidocaldarius 104-IA is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from acid hot spring.

Gram-positive rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 1090

LMG 7119,ATCC 27009,IFO 15652,JCM 5260,NCIB 11725,CIP 106131,CCUG 28521,NRRL B-14509,IMET 11356,CCRC 14685,KCTC 1825,NCIMB 11725,LMD 89.167,CECT 4328,NBRC 15652,HAMBI 2071,HAMBI 2073,NCCB 89167,BCRC 14685,CCT 2490,DSM 446

@ref 20215

Last LPSN update 2026-02-09 11:17:52

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Alicyclobacillaceae

Genus Alicyclobacillus

Species Alicyclobacillus acidocaldarius subsp. acidocaldarius

Full scientific name Alicyclobacillus acidocaldarius subsp. acidocaldarius (Darland and Brock 1971) Nicolaus et al. 2024

BacDive ID	Other strains from **Alicyclobacillus acidocaldarius subsp. acidocaldarius** (7)	Type strain
417	A. acidocaldarius subsp. acidocaldarius 115-8, DSM 448
418	A. acidocaldarius subsp. acidocaldarius GD-IA, DSM 449
419	A. acidocaldarius subsp. acidocaldarius GD-3B, DSM 451
420	A. acidocaldarius subsp. acidocaldarius 470-B, DSM 452
421	A. acidocaldarius subsp. acidocaldarius HT-1, DSM 453, JCM 5261, ATCC 43030
422	A. acidocaldarius subsp. acidocaldarius HT-2, DSM 454
423	A. acidocaldarius subsp. acidocaldarius HT-4, DSM 455

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
116015	positive	rod-shaped

Colony morphology

49268

Incubation period4 days

Multimedia

@ref:	66793
multimedia content:	EM_DSM_446_1.jpg
multimedia.multimedia content:	EM_DSM_446_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
211	ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)	Medium recipe at MediaDive	Name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402) Composition: Agar 15.0 g/l Glucose 5.0 g/l KH2PO4 3.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
40298	MEDIUM 317 - for Alicyclobacillus acidocaldarius		Distilled water make up to (500.000 ml);Magnesium sulphate heptahydrate (0.250 g);Calcium chloride dihydrate (0.070 g);Glucose (1.000 g);Yeast extract (1.000 g);Ammonium sulphate (1.300 g);Potassium di-hydrogen phosphate (0.370 g);Ferric III chloride hexa
116015	CIP Medium 317	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
211	positive	growth	60
40298	positive	growth	55
49268	positive	growth	56
67770	positive	growth	60
116015	positive	growth	45-55
116015	negative	growth	10
116015	negative	growth	25
116015	negative	growth	37
116015	negative	growth	41

Culture pH

@ref	Ability	Type	PH
116015		growth	6

Physiology and metabolism

Oxygen tolerance

49268

Oxygen toleranceaerobe

Compound production

@ref	Compound
211	amylase, thermoacidophilic
211	restriction endonuclease BacI (SacII)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68368	27613 ChEBI	amygdalin	-	fermentation	from API 20E
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68368	17634 ChEBI	D-glucose	-	fermentation	from API 20E
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68368	16899 ChEBI	D-mannitol	-	fermentation	from API 20E
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
116015	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68368	30849 ChEBI	L-arabinose	-	fermentation	from API 20E
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68368	62345 ChEBI	L-rhamnose	-	fermentation	from API 20E
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68368	28053 ChEBI	melibiose	-	fermentation	from API 20E
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68368	17268 ChEBI	myo-inositol	-	fermentation	from API 20E
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
116015	17632 ChEBI	nitrate	-	reduction
116015	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	+	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68368	30911 ChEBI	sorbitol	-	fermentation	from API 20E
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
116015	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
116015	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	+			from API 20E
116015	17234 ChEBI	glucose		-
68368	35581 ChEBI	indole			-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
116015	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
116015	beta-galactosidase	-	3.2.1.23
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
116015	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68368	cytochrome oxidase	-	1.9.3.1	from API 20E
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
116015	gamma-glutamyltransferase	-	2.3.2.2
116015	gelatinase	-
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
116015	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116015	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
116015	oxidase	-
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
116015	urease	-	3.5.1.5
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
49268	+	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116015	-	-	+	+	-	+	+	-	+	-	+	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
49268	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Acidic	-
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
211	acid hot spring		USA	USA	North America
49268	Acid hot spring		USA	USA	North America
67770	Acid hot spring	Nymph Creek, Yellowstone National Park, WY	USA	USA	North America
116015	Environment, Acid hot spring

Taxonmaps

69479

Global distribution of 16S sequence AB271754 (>99% sequence identity) for Alicyclobacillus acidocaldarius subclade from Microbeatlas

Aquatic Samples	244
Soil Samples	488
Animal Samples	1218
Plant Samples	275
Total Samples	2225

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
211	1	Risk group (German classification) According to TRBA 466
116015	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2428v1 assembly for Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	complete	GCA_000024285	521098.5	644736325	521098	93.92

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Alicyclobacillus acidocaldarius subsp. acidocaldarius gene for 16S rRNA, partial sequence	AB006917	262	521098
20218	Alicyclobacillus acidocaldarius subsp. acidocaldarius gene for 16S rRNA, strain: ATCC 27009	AB042056	1520	521098
20218	Alicyclobacillus acidocaldarius gene for 16S rRNA, partial sequence	AB271754	1463	405212
20218	Alicyclobacillus acidocaldarius 16S rRNA gene, strain DSM 446	AJ496806	1507	521098
20218	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 isolate Aac1 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723605	347	521098
20218	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 isolate Aac2 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723606	346	521098
20218	B.acidocaldarius 16S rRNA	X60742	1548	521098

GC content

@ref	GC-content (mol%)	Method
211	62.3	Buoyant density centrifugation (BD)
211	60.3	thermal denaturation, midpoint method (Tm)
67770	62.2	Buoyant density centrifugation (BD)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
PATRIC: 521098.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	74.20	no
125439	motility	BacteriaNetⓘ	yes	60.30	no
125439	gram_stain	BacteriaNetⓘ	negative	85.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	71.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 DSM 446
NCBI: GCA_000024285.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	63.48	no
125438	anaerobic	anaerobicⓘ	no	93.20	yes
125438	aerobic	aerobicⓘ	yes	77.68	no
125438	spore-forming	spore-formingⓘ	yes	85.94	no
125438	thermophilic	thermophileⓘ	yes	62.79	no
125438	flagellated	motile2+ⓘ	yes	71.29	no

Literature

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	Validation List no. 222. Valid publication of new names and new combinations effectively published outside the IJSEM.	Oren A, Goker M.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006640	2025
Genetics	Draft genome sequence of the Alicyclobacillus acidocaldarius strain LH1, isolated from a highly acidic solfatara field.	Fuentes-Tobar FJ, Torres-Ponce N, Blamey JM, Cuadros-Orellana S.	Microbiol Resour Announc	10.1128/mra.00776-24	2025
Genetics	Genomic insight and physiological characterization of thermoacidophilic Alicyclobacillus isolated from Yellowstone National Park.	Kim HW, Kim NK, Phillips APR, Parker DA, Liu P, Whitaker RJ, Rao CV, Mackie RI.	Front Microbiol	10.3389/fmicb.2023.1232587	2023
Enzymology	Thermophilic bacteria are potential sources of novel Rieske non-heme iron oxygenases.	Chakraborty J, Suzuki-Minakuchi C, Okada K, Nojiri H.	AMB Express	10.1186/s13568-016-0318-5	2017
Genetics	Characterization and Genome Study of a Newly Isolated Temperate Phage Belonging to a New Genus Targeting Alicyclobacillus acidoterrestris.	Shymialevich D, Wojcicki M, Swider O, Srednicka P, Sokolowska B.	Genes (Basel)	10.3390/genes14061303	2023
Enzymology	Genetic Heterogeneity of Alicyclobacillus Strains Revealed by RFLP Analysis of vdc Region and rpoB Gene.	Dekowska A, Niezgoda J, Sokolowska B.	Biomed Res Int	10.1155/2018/9608756	2018
	Draft Genome Sequence of Alicyclobacillus acidoterrestris Strain ATCC 49025.	Shemesh M, Pasvolsky R, Sela N, Green SJ, Zakin V.	Genome Announc	10.1128/genomea.00638-13	2013
Phylogeny	Draft genome sequence of Alicyclobacillus hesperidum strain URH17-3-68.	Wang P, Li L, Chen X, Jiang N, Liu G, Chen L, Xu J, Song H, Chen Z, Ma Y.	J Bacteriol	10.1128/jb.01612-12	2012
	Building a Geochemical View of Microbial Salt Tolerance: Halophilic Adaptation of Marinococcus in a Natural Magnesium Sulfate Brine.	Fox-Powell MG, Cockell CS.	Front Microbiol	10.3389/fmicb.2018.00739	2018
Genetics	Unveiling the complete genome sequence of Alicyclobacillus acidoterrestris DSM 3922T, a taint-producing strain.	Leonardo IC, Barreto Crespo MT, Gaspar FB.	G3 (Bethesda)	10.1093/g3journal/jkac225	2022
	Discovery of lignin-transforming bacteria and enzymes in thermophilic environments using stable isotope probing.	Levy-Booth DJ, Navas LE, Fetherolf MM, Liu LY, Dalhuisen T, Renneckar S, Eltis LD, Mohn WW.	ISME J	10.1038/s41396-022-01241-8	2022
Metabolism	Function, distribution, and annotation of characterized cellulases, xylanases, and chitinases from CAZy.	Nguyen STC, Freund HL, Kasanjian J, Berlemont R.	Appl Microbiol Biotechnol	10.1007/s00253-018-8778-y	2018
	Constructing phylogenetic trees using interacting pathways.	Wan P, Che D.	Bioinformation	10.6026/97320630009363	2013
Enzymology	Expression of an extremely acidic beta-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence.	Bai Y, Wang J, Zhang Z, Shi P, Luo H, Huang H, Luo C, Yao B.	Microb Cell Fact	10.1186/1475-2859-9-33	2010
Metabolism	Structural and functional analysis of the solute-binding protein UspC from Mycobacterium tuberculosis that is specific for amino sugars.	Fullam E, Prokes I, Futterer K, Besra GS.	Open Biol	10.1098/rsob.160105	2016
Metabolism	Identification and Characterization of a New 7-Aminocephalosporanic Acid Deacetylase from Thermophilic Bacterium Alicyclobacillus tengchongensis.	Ding JM, Yu TT, Han NY, Yu JL, Li JJ, Yang YJ, Tang XH, Xu B, Zhou JP, Tang HZ, Huang ZX.	J Bacteriol	10.1128/jb.00471-15	2016
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Metabolism	Chemical and physical characterization of four interfacial-active rhamnolipids from Pseudomonas spec. DSM 2874 grown on n-alkanes.	Syldatk C, Lang S, Wagner F, Wray V, Witte L.	Z Naturforsch C Biosci	10.1515/znc-1985-1-212	1985
	Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.	San Martin-Uriz P, Mirete S, Alcolea PJ, Gomez MJ, Amils R, Gonzalez-Pastor JE.	PLoS One	10.1371/journal.pone.0095041	2014
Genetics	On the molecular mechanism of GC content variation among eubacterial genomes.	Wu H, Zhang Z, Hu S, Yu J.	Biol Direct	10.1186/1745-6150-7-2	2012
Genetics	Microbial diversity and metabolic networks in acid mine drainage habitats.	Mendez-Garcia C, Pelaez AI, Mesa V, Sanchez J, Golyshina OV, Ferrer M.	Front Microbiol	10.3389/fmicb.2015.00475	2015
	Biochemical diversity of carboxyl esterases and lipases from Lake Arreo (Spain): a metagenomic approach.	Martinez-Martinez M, Alcaide M, Tchigvintsev A, Reva O, Polaina J, Bargiela R, Guazzaroni ME, Chicote A, Canet A, Valero F, Rico Eguizabal E, Guerrero Mdel C, Yakunin AF, Ferrer M.	Appl Environ Microbiol	10.1128/aem.00240-13	2013
	Distribution of genes encoding nucleoid-associated protein homologs in plasmids.	Takeda T, Yun CS, Shintani M, Yamane H, Nojiri H.	Int J Evol Biol	10.4061/2011/685015	2011
	Annotation of Protein Domains Reveals Remarkable Conservation in the Functional Make up of Proteomes Across Superkingdoms.	Nasir A, Naeem A, Khan MJ, Nicora HD, Caetano-Anolles G.	Genes (Basel)	10.3390/genes2040869	2011
Metabolism	Comparative genome analysis of central nitrogen metabolism and its control by GlnR in the class Bacilli.	Groot Kormelink T, Koenders E, Hagemeijer Y, Overmars L, Siezen RJ, de Vos WM, Francke C.	BMC Genomics	10.1186/1471-2164-13-191	2012
	Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius	Yuan T, Yang P, Wang Y, Meng K, Luo H, Zhang W, Wu N, Fan Y, Yao B.	Biotechnol Lett		2008
	Class II terpene cyclases: structures, mechanisms, and engineering.	Pan X, Rudolf JD, Dong LB.	Nat Prod Rep	10.1039/d3np00033h	2024
Metabolism	Polarity Alteration of a Calcium Site Induces a Hydrophobic Interaction Network and Enhances Cel9A Endoglucanase Thermostability.	Wang HJ, Hsiao YY, Chen YP, Ma TY, Tseng CP.	Appl Environ Microbiol	10.1128/aem.03326-15	2016
Metabolism	Maltose and maltodextrin transport in the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius is mediated by a high-affinity transport system that includes a maltose binding protein tolerant to low pH.	Hulsmann A, Lurz R, Scheffel F, Schneider E.	J Bacteriol	10.1128/jb.182.22.6292-6301.2000	2000
	Glycoside hydrolase gene transcription by Alicyclobacillus acidocaldarius during growth on wheat arabinoxylan and monosaccharides: a proposed xylan hydrolysis mechanism.	Lee BD, Apel WA, Sheridan PP, DeVeaux LC	Biotechnol Biofuels	10.1186/s13068-018-1110-3	2018
Genetics	Complete genome sequence of Alicyclobacillus acidocaldarius type strain (104-IA).	Mavromatis K, Sikorski J, Lapidus A, Glavina Del Rio T, Copeland A, Tice H, Cheng JF, Lucas S, Chen F, Nolan M, Bruce D, Goodwin L, Pitluck S, Ivanova N, Ovchinnikova G, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Chain P, Meincke L, Sims D, Chertkov O, Han C, Brettin T, Detter JC, Wahrenburg C, Rohde M, Pukall R, Goker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Klenk HP, Kyrpides NC	Stand Genomic Sci	10.4056/sigs.591104	2010
Enzymology	A novel thermoacidophilic cellulase from Alicyclobacillus acidocaldarius.	Morana A, Esposito A, Maurelli L, Ruggiero G, Ionata E, Rossi M, La Cara F	Protein Pept Lett	10.2174/092986608785849209	2008
Enzymology	Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius.	Yuan T, Yang P, Wang Y, Meng K, Luo H, Zhang W, Wu N, Fan Y, Yao B	Biotechnol Lett	10.1007/s10529-007-9551-y	2007
Enzymology	Characterization of a beta-glycosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius.	Di Lauro B, Rossi M, Moracci M	Extremophiles	10.1007/s00792-005-0500-1	2006
Enzymology	Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009.	Eckert K, Zielinski F, Lo Leggio L, Schneider E	Appl Microbiol Biotechnol	10.1007/s00253-002-1131-4	2002
Phylogeny	Identification of thermoacidophilic bacteria and a new Alicyclobacillus genomic species isolated from acidic environments in Japan.	Goto K, Tanimoto Y, Tamura T, Mochida K, Arai D, Asahara M, Suzuki M, Tanaka H, Inagaki K	Extremophiles	10.1007/s00792-001-0262-3	2002
Enzymology	Gene cloning, nucleotide sequence and biochemical properties of a cytoplasmic cyclomaltodextrinase (neopullulanase) from Alicyclobacillus acidocaldarius, reclassification of a group of enzymes.	Matzke J, Herrmann A, Schneider E, Bakker EP	FEMS Microbiol Lett	10.1111/j.1574-6968.2000.tb08933.x	2000
Enzymology	Acidostable and acidophilic proteins: the example of the alpha-amylase from Alicyclobacillus acidocaldarius.	Matzke J, Schwermann B, Bakker EP	Comp Biochem Physiol A Physiol	10.1016/s0300-9629(97)00008-x	1997
Stress	Biochemical identification of a lipoprotein with maltose-binding activity in the thermoacidophilic Gram-positive bacterium Alicyclobacillus acidocaldarius.	Herrmann A, Schlosser A, Schmid R, Schneider E	Res Microbiol	10.1016/s0923-2508(97)85120-0	1996
Enzymology	Purification, properties and structural aspects of a thermoacidophilic alpha-amylase from Alicyclobacillus acidocaldarius atcc 27009. Insight into acidostability of proteins.	Schwermann B, Pfau K, Liliensiek B, Schleyer M, Fischer T, Bakker EP	Eur J Biochem	10.1111/j.1432-1033.1994.00981.x	1994
Genetics	Bacillus ayatagriensis sp. nov., a novel plant growth-promoting rhizobacteria strain isolated from mulberry rhizosphere.	Das S, Mondal R, Mandal P, Kurt H, Chakraborty J, Islam MM, Sarkar B, Shaw S, Manna S, Arabaci N, Jangid K, Sadat A, Gangopadhyay D, Kati A, Mandal AK.	Sci Rep	10.1038/s41598-025-05508-w	2025
Phylogeny	Reclassification of 'Sulfobacillus thermosulfidooxidans subsp. thermotolerans' strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus.	Karavaiko GI, Bogdanova TI, Tourova TP, Kondrat'eva TF, Tsaplina IA, Egorova MA, Krasil'nikova EN, Zakharchuk LM	Int J Syst Evol Microbiol	10.1099/ijs.0.63300-0	2005

References

#211	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 446
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40298	; Curators of the CIP;
#49268	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28521
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116015	Collection of Institut Pasteur ; Curators of the CIP; CIP 106131
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alicyclobacillus acidocaldarius subsp. acidocaldarius (CCUG 28521, NRRL B-14509, DSM 446)

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