Desulfonatronum thiosulfatophilum (DSM 21338, UNIQEM U757, ASO4-2)

Name: Desulfonatronum thiosulfatophilum (DSM 21338, UNIQEM U757, ASO4-2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 4067

Type strain

Strain Designation ASO4-2

Culture col. no. DSM 21338 UNIQEM U757 ASO4-2

NCBI tax ID(s) 617002

Special Collections DSMZ

Links

Desulfonatronum thiosulfatophilum ASO4-2 is an anaerobe, mesophilic prokaryote that was isolated from sediments from hypersaline soda lake.

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405461

DSM 21338

@ref 20215

Last LPSN update 2025-12-16 09:47:43

Domain Pseudomonadati

Phylum Thermodesulfobacteriota

Class Desulfovibrionia

Order Desulfovibrionales

Family Desulfonatronaceae

Genus Desulfonatronum

Species Desulfonatronum thiosulfatophilum

Full scientific name Desulfonatronum thiosulfatophilum Sorokin et al. 2011

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	96.6

Colony morphology

15624

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
15624	DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166)		Medium recipe at MediaDive	Name: DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166) Composition: Na2CO3 21.9342 g/l NaHCO3 7.97607 g/l NaCl 5.98205 g/l Na2S2O3 x 5 H2O 4.98504 g/l Na-formate 3.38983 g/l Na2SO4 2.79163 g/l K2HPO4 0.997009 g/l Na-pyruvate 0.548355 g/l Na2S x 9 H2O 0.239282 g/l MgCl2 x 6 H2O 0.199402 g/l NH4Cl 0.199402 g/l Yeast extract 0.0498504 g/l EDTA 0.00498504 g/l FeSO4 x 7 H2O 0.00219342 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000199402 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnSO4 x 7 H2O 9.97009e-05 g/l Thiamine HCl 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l NiCl2 x 6 H2O 2.99103e-05 g/l MnCl2 x 4 H2O 2.99103e-05 g/l H3BO3 2.99103e-05 g/l CuCl2 x 2 H2O 2.99103e-05 g/l Na2MoO4 x 2 H2O 2.99103e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15624	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

15624

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125439		93.5

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	acetoin degradation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	chorismate metabolism	100	9 of 9
66794	glycogen metabolism	100	5 of 5
66794	L-lactaldehyde degradation	100	3 of 3
66794	kanosamine biosynthesis II	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	methylglyoxal degradation	100	5 of 5
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	starch degradation	90	9 of 10
66794	lipid A biosynthesis	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
66794	pentose phosphate pathway	81.82	9 of 11
66794	hydrogen production	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	phenylalanine metabolism	76.92	10 of 13
66794	CMP-KDO biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	purine metabolism	74.47	70 of 94
66794	vitamin B12 metabolism	73.53	25 of 34
66794	peptidoglycan biosynthesis	73.33	11 of 15
66794	alanine metabolism	72.41	21 of 29
66794	NAD metabolism	72.22	13 of 18
66794	pyrimidine metabolism	71.11	32 of 45
66794	threonine metabolism	70	7 of 10
66794	methionine metabolism	69.23	18 of 26
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	flavin biosynthesis	66.67	10 of 15
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	heme metabolism	64.29	9 of 14
66794	proline metabolism	63.64	7 of 11
66794	metabolism of disaccharids	63.64	7 of 11
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	isoleucine metabolism	62.5	5 of 8
66794	sulfate reduction	61.54	8 of 13
66794	glutamate and glutamine metabolism	60.71	17 of 28
66794	non-pathway related	60.53	23 of 38
66794	oxidative phosphorylation	60.44	55 of 91
66794	factor 420 biosynthesis	60	3 of 5
66794	glycolysis	58.82	10 of 17
66794	citric acid cycle	57.14	8 of 14
66794	serine metabolism	55.56	5 of 9
66794	nitrate assimilation	55.56	5 of 9
66794	vitamin B6 metabolism	54.55	6 of 11
66794	isoprenoid biosynthesis	53.85	14 of 26
66794	leucine metabolism	53.85	7 of 13
66794	ethanol fermentation	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	Entner Doudoroff pathway	50	5 of 10
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	butanoate fermentation	50	2 of 4
66794	glycine metabolism	50	5 of 10
66794	arginine metabolism	50	12 of 24
66794	suberin monomers biosynthesis	50	1 of 2
66794	gluconeogenesis	50	4 of 8
66794	ketogluconate metabolism	50	4 of 8
66794	cysteine metabolism	50	9 of 18
66794	lysine metabolism	47.62	20 of 42
66794	tryptophan metabolism	47.37	18 of 38
66794	histidine metabolism	44.83	13 of 29
66794	CO2 fixation in Crenarchaeota	44.44	4 of 9
66794	tyrosine metabolism	42.86	6 of 14
66794	glutathione metabolism	42.86	6 of 14
66794	propanol degradation	42.86	3 of 7
66794	O-antigen biosynthesis	40	2 of 5
66794	glycine betaine biosynthesis	40	2 of 5
66794	D-cycloserine biosynthesis	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	degradation of pentoses	35.71	10 of 28
66794	polyamine pathway	34.78	8 of 23
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	cyanate degradation	33.33	1 of 3
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	methanogenesis from CO2	33.33	4 of 12
66794	octane oxidation	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	urea cycle	30.77	4 of 13
66794	coenzyme M biosynthesis	30	3 of 10
66794	phenol degradation	30	6 of 20
66794	ascorbate metabolism	27.27	6 of 22
66794	lipid metabolism	25.81	8 of 31
66794	carnitine metabolism	25	2 of 8
66794	toluene degradation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	degradation of sugar alcohols	25	4 of 16
66794	biotin biosynthesis	25	1 of 4
66794	degradation of sugar acids	24	6 of 25

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Alkaline	-
#Condition	#Saline	-
#Environmental	#Aquatic	#Non-marine Saline and Alkaline

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
15624	sediments from hypersaline soda lake	Altai, south-eastern Siberia, Kulunda Steppe, soda lake Picturesque	Russia	RUS	Asia

Taxonmaps

69479

Global distribution of 16S sequence FJ469578 (>99% sequence identity) for Desulfonatronum thiosulfatophilum subclade from Microbeatlas

Aquatic Samples	105
Soil Samples	18
Animal Samples	6
Plant Samples
Total Samples	129

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15624	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	IMG-taxon 2596583601 annotated assembly for Desulfonatronum thiosulfatophilum ASO4-2	contig	GCA_900104215	617002.3	2596583601	617002	71.13

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15624	Desulfonatronum thiosulfatophilum strain ASO4-2 16S ribosomal RNA gene, partial sequence	FJ469578	1522		617002

GC content

@ref	GC-content (mol%)	Method
15624	57.0	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Desulfonatronum thiosulfatophilum ASO4-2
NCBI: GCA_900104215

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	93.50	no
125439	motility	BacteriaNetⓘ	yes	61.30	no
125439	gram_stain	BacteriaNetⓘ	negative	96.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	89.90	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Gut microbiome composition and diversity of wild-caught and hatchery-bred milkfish (Chanos chanos) fry.	Silvederio GXL, Javellana TF, Genciana ABN, Fontanilla MAG, Traifalgar RFM, Huervana FH, Del Castillo CS.	J Genet Eng Biotechnol	10.1016/j.jgeb.2025.100520	2025
Genetics	Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism.	Yvenou S, Allioux M, Slobodkin A, Slobodkina G, Jebbar M, Alain K.	Microorganisms	10.3390/microorganisms10010060	2021
Metabolism	Identification of an apiosyltransferase in the plant pathogen Xanthomonas pisi.	Smith JA, Bar-Peled M.	PLoS One	10.1371/journal.pone.0206187	2018
	The Shifts of Diazotrophic Communities in Spring and Summer Associated with Coral Galaxea astreata, Pavona decussata, and Porites lutea.	Zhang Y, Yang Q, Ling J, Van Nostrand JD, Shi Z, Zhou J, Dong J.	Front Microbiol	10.3389/fmicb.2016.01870	2016
	A novel signal transduction protein: Combination of solute binding and tandem PAS-like sensor domains in one polypeptide chain.	Wu R, Wilton R, Cuff ME, Endres M, Babnigg G, Edirisinghe JN, Henry CS, Joachimiak A, Schiffer M, Pokkuluri PR.	Protein Sci	10.1002/pro.3134	2017
Phylogeny	Desulfonatronum parangueonense sp. nov., a sulfate-reducing bacterium isolated from sediment of an alkaline crater lake.	Perez Bernal MF, Souza Brito EM, Bartoli M, Aube J, Fardeau ML, Cuevas Rodriguez G, Ollivier B, Guyoneaud R, Hirschler-Rea A.	Int J Syst Evol Microbiol	10.1099/ijsem.0.002399	2017
Phylogeny	Culturable diversity of lithotrophic haloalkaliphilic sulfate-reducing bacteria in soda lakes and the description of Desulfonatronum thioautotrophicum sp. nov., Desulfonatronum thiosulfatophilum sp. nov., Desulfonatronovibrio thiodismutans sp. nov., and Desulfonatronovibrio magnus sp. nov.	Sorokin DY, Tourova TP, Kolganova TV, Detkova EN, Galinski EA, Muyzer G.	Extremophiles	10.1007/s00792-011-0370-7	2011

References

#15624	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21338
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Desulfonatronum thiosulfatophilum (DSM 21338, UNIQEM U757, ASO4-2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900104215

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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