Desulfobotulus alkaliphilus (DSM 22078, UNIQEM U759, ASO4-4)

Name: Desulfobotulus alkaliphilus (DSM 22078, UNIQEM U759, ASO4-4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 4000

Type strain

Strain Designation ASO4-4

Culture col. no. DSM 22078 UNIQEM U759 ASO4-4

NCBI tax ID(s) 622671

Special Collections DSMZ

History <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ASO4-4 <- D. Y. Sorokin {2008}

Links

Desulfobotulus alkaliphilus ASO4-4 is an anaerobe bacterium that was isolated from sediments from a hypersaline soda lake.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 403753

DSM 22078

@ref 20215

Last LPSN update 2026-02-09 11:18:37

Domain Bacteria

Phylum Thermodesulfobacteriota

Class Desulfobacteria

Order Desulfobacterales

Family Desulfobacteraceae

Genus Desulfobotulus

Species Desulfobotulus alkaliphilus

Full scientific name Desulfobotulus alkaliphilus Sorokin et al. 2010

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	99.7
125438	negative	96.233

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16115	DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166)		Medium recipe at MediaDive	Name: DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166) Composition: Na2CO3 21.9342 g/l NaHCO3 7.97607 g/l NaCl 5.98205 g/l Na2S2O3 x 5 H2O 4.98504 g/l Na-formate 3.38983 g/l Na2SO4 2.79163 g/l K2HPO4 0.997009 g/l Na-pyruvate 0.548355 g/l Na2S x 9 H2O 0.239282 g/l MgCl2 x 6 H2O 0.199402 g/l NH4Cl 0.199402 g/l Yeast extract 0.0498504 g/l EDTA 0.00498504 g/l FeSO4 x 7 H2O 0.00219342 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000199402 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnSO4 x 7 H2O 9.97009e-05 g/l Thiamine HCl 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l NiCl2 x 6 H2O 2.99103e-05 g/l MnCl2 x 4 H2O 2.99103e-05 g/l H3BO3 2.99103e-05 g/l CuCl2 x 2 H2O 2.99103e-05 g/l Na2MoO4 x 2 H2O 2.99103e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
16115	positive	growth	30

Physiology and metabolism

Oxygen tolerance

16115

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125439		98.2

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	propanol degradation	100	7 of 7
66794	chorismate metabolism	100	9 of 9
66794	1,4-dihydroxy-6-naphthoate biosynthesis	100	6 of 6
66794	methylglyoxal degradation	100	5 of 5
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	CMP-KDO biosynthesis	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	ethanol fermentation	100	2 of 2
66794	L-lactaldehyde degradation	100	3 of 3
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	palmitate biosynthesis	100	22 of 22
66794	sulfopterin metabolism	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	biotin biosynthesis	100	4 of 4
66794	acetoin degradation	100	3 of 3
66794	threonine metabolism	90	9 of 10
66794	valine metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	phenylalanine metabolism	84.62	11 of 13
66794	peptidoglycan biosynthesis	80	12 of 15
66794	propionate fermentation	80	8 of 10
66794	flavin biosynthesis	80	12 of 15
66794	photosynthesis	78.57	11 of 14
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	lipid A biosynthesis	77.78	7 of 9
66794	purine metabolism	76.6	72 of 94
66794	glycolysis	76.47	13 of 17
66794	toluene degradation	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	ppGpp biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	alanine metabolism	72.41	21 of 29
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	glutamate and glutamine metabolism	71.43	20 of 28
66794	leucine metabolism	69.23	9 of 13
66794	formaldehyde oxidation	66.67	2 of 3
66794	NAD metabolism	66.67	12 of 18
66794	d-mannose degradation	66.67	6 of 9
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	pyrimidine metabolism	64.44	29 of 45
66794	heme metabolism	64.29	9 of 14
66794	citric acid cycle	64.29	9 of 14
66794	proline metabolism	63.64	7 of 11
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	vitamin B1 metabolism	61.54	8 of 13
66794	polyamine pathway	60.87	14 of 23
66794	tryptophan metabolism	60.53	23 of 38
66794	hydrogen production	60	3 of 5
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	lipid metabolism	58.06	18 of 31
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	methionine metabolism	57.69	15 of 26
66794	serine metabolism	55.56	5 of 9
66794	non-pathway related	55.26	21 of 38
66794	histidine metabolism	55.17	16 of 29
66794	lysine metabolism	54.76	23 of 42
66794	vitamin B6 metabolism	54.55	6 of 11
66794	arginine metabolism	54.17	13 of 24
66794	oxidative phosphorylation	51.65	47 of 91
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	myo-inositol biosynthesis	50	5 of 10
66794	gluconeogenesis	50	4 of 8
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	kanosamine biosynthesis II	50	1 of 2
66794	suberin monomers biosynthesis	50	1 of 2
66794	glycogen biosynthesis	50	2 of 4
66794	sulfate reduction	46.15	6 of 13
66794	urea cycle	46.15	6 of 13
66794	nitrate assimilation	44.44	4 of 9
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	tyrosine metabolism	42.86	6 of 14
66794	glutathione metabolism	42.86	6 of 14
66794	O-antigen biosynthesis	40	2 of 5
66794	Entner Doudoroff pathway	40	4 of 10
66794	factor 420 biosynthesis	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	coenzyme M biosynthesis	40	4 of 10
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	degradation of hexoses	38.89	7 of 18
66794	ketogluconate metabolism	37.5	3 of 8
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	pentose phosphate pathway	36.36	4 of 11
66794	cyanate degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	cysteine metabolism	33.33	6 of 18
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	methane metabolism	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	degradation of pentoses	32.14	9 of 28
66794	degradation of sugar alcohols	31.25	5 of 16
66794	phosphatidylethanolamine bioynthesis	30.77	4 of 13
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	ascorbate metabolism	27.27	6 of 22
66794	phenol degradation	25	5 of 20
66794	carnitine metabolism	25	2 of 8
66794	cyclohexanol degradation	25	1 of 4
66794	bile acid biosynthesis, neutral pathway	23.53	4 of 17
66794	vitamin B12 metabolism	20.59	7 of 34

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Saline	-
#Condition	#Alkaline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
16115	sediments from a hypersaline soda lake	Altai, Kulunda Steppe	Russia	RUS	Asia

Taxonmaps

69479

Global distribution of 16S sequence FJ788523 (>99% sequence identity) for Desulfobotulus alkaliphilus from Microbeatlas

Aquatic Samples	197
Soil Samples	29
Animal Samples	22
Plant Samples
Total Samples	248

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16115	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM783043v1 assembly for Desulfobotulus alkaliphilus ASO4-4	scaffold	GCA_007830435	622671.3	2596583602	622671	49.98

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16115	Desulfobotulus alkaliphilus strain ASO4-4 16S ribosomal RNA gene, partial sequence	FJ788523	1392		622671

GC content

@ref	GC-content (mol%)	Method
16115	51.3	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Desulfobotulus alkaliphilus ASO4-4
NCBI: GCA_007830435

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.20	no
125439	motility	BacteriaNetⓘ	yes	70.50	no
125439	gram_stain	BacteriaNetⓘ	negative	99.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	81.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Desulfobotulus alkaliphilus ASO4-4
NCBI: GCA_007830435.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	96.23	no
125438	anaerobic	anaerobicⓘ	yes	89.36	yes
125438	aerobic	aerobicⓘ	no	85.09	no
125438	spore-forming	spore-formingⓘ	no	85.99	no
125438	thermophilic	thermophileⓘ	no	90.50	yes
125438	flagellated	motile2+ⓘ	yes	73.17	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	The electrically conductive pili of Geobacter species are a recently evolved feature for extracellular electron transfer.	Holmes DE, Dang Y, Walker DJF, Lovley DR.	Microb Genom	10.1099/mgen.0.000072	2016
	Anthropogenic impact on diazotrophic diversity in the mangrove rhizosphere revealed by nifH pyrosequencing.	Jing H, Xia X, Liu H, Zhou Z, Wu C, Nagarajan S.	Front Microbiol	10.3389/fmicb.2015.01172	2015
Phylogeny	Desulfobotulus mexicanus sp. nov., a novel sulfate-reducing bacterium isolated from the sediment of an alkaline crater lake in Mexico.	Perez-Bernal MF, Brito EMS, Bartoli M, Aube J, Ollivier B, Guyoneaud R, Hirschler-Rea A.	Int J Syst Evol Microbiol	10.1099/ijsem.0.004159	2020
Metabolism	Propionate and butyrate dependent bacterial sulfate reduction at extremely haloalkaline conditions and description of Desulfobotulus alkaliphilus sp. nov.	Sorokin DY, Detkova EN, Muyzer G.	Extremophiles	10.1007/s00792-009-0288-5	2010

References

#16115	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22078
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Desulfobotulus alkaliphilus (DSM 22078, UNIQEM U759, ASO4-4)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_007830435

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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