Name: Brachybacterium muris C3H-21
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 3914

Type strain:

Species: Brachybacterium muris

Strain Designation: C3H-21, C3H 21-2

Culture col. no.: DSM 15460, CCM 7047, JCM 14117, CCUG 50159, CCUG 50352, CIP 108210, DSM 14560

Strain history: CIP <- 2003, CCM <- H.J. Busse, Vienna Univ., Vienna, Austria <- S. Buczolits: strain C3H 21-2

NCBI tax ID(s): 219301 (species)

SI-ID 127984

CCM 7047, DSM 14560, DSM 15460, CCUG 50159, CIP 108210, CCUG 50352, JCM 14117

Other strains from Brachybacterium muris

B. muris CCUG 57901 B
B. muris CCUG 61799
B. muris CCUG 69386

Section

Brachybacterium muris C3H-21 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from liver of mice.

Gram-positive
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Dermabacteraceae*
	Genus	*Brachybacterium*
	Species	**Brachybacterium muris**
	Full Scientific Name (LPSN)	Brachybacterium muris Buczolits et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #121199]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #121199]	Cell shape	coccus-shaped

[Ref.: #121199]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5937]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #5937]

Culture medium growth

[Ref.: #5937]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5937]

Culture medium composition

expand / minimize

[Ref.: #5937]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #5937]

Culture medium growth

[Ref.: #5937]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5937]

Culture medium composition

expand / minimize

[Ref.: #33912]

Culture medium

MEDIUM 328- for nutrient agar

[Ref.: #33912]

Culture medium growth

[Ref.: #33912]

Culture medium composition

expand / minimize

[Ref.: #121199]

Culture medium

CIP Medium 328

[Ref.: #121199]

Culture medium growth

[Ref.: #121199]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5937]		growth	28 °C
[Ref.: #33912]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #121199]		growth	25-37 °C
[Ref.: #121199]	no	growth	10 °C
[Ref.: #121199]	no	growth	41 °C
[Ref.: #121199]	no	growth	45 °C

[Ref.: #5937]	Temperature range	mesophilic
[Ref.: #33912]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121199]	Temperature range	mesophilic
[Ref.: #121199]	Temperature range	psychrophilic
[Ref.: #121199]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121199]

Oxygen tolerance

obligate aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.933

	Salt	Tested relation		Salt conc.
[Ref.: #121199]	NaCl		growth	0-6	%
[Ref.: #121199]	NaCl	no	growth	8	%
[Ref.: #121199]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121199]	citrate ChEBI	-	carbon source
[Ref.: #121199]	esculin ChEBI	+	hydrolysis
[Ref.: #121199]	hippurate ChEBI	-	hydrolysis
[Ref.: #121199]	nitrate ChEBI	-	reduction
[Ref.: #121199]	nitrate ChEBI	-	respiration
[Ref.: #121199]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121199]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #121199]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #121199]		acetoin ChEBI	-
[Ref.: #121199]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #121199]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #121199]	amylase	+
[Ref.: #121199]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121199]	caseinase	-	3.4.21.50
[Ref.: #121199]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121199]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121199]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #121199]	gelatinase	+/-
[Ref.: #121199]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121199]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121199]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #121199]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121199]	oxidase	-
[Ref.: #121199]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #121199]	tryptophan deaminase	-
[Ref.: #121199]	tween esterase	-
[Ref.: #121199]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121199]	10987	-	-	+	+	-	+	-	-	+	-	-	-	-	+	-	-	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5937]	Sample type/isolated from	liver of mice
[Ref.: #5937]	Geographic location (country and/or sea, region)	Vienna
[Ref.: #5937]	Country	Austria
[Ref.: #5937]	Country ISO 3 Code	AUT
[Ref.: #5937]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Liver of a laboratory mouse strain

[Ref.: #121199]	Sample type/isolated from	Animal, Mouse, liver
[Ref.: #121199]	Geographic location (country and/or sea, region)	Vienna
[Ref.: #121199]	Country	Austria
[Ref.: #121199]	Country ISO 3 Code	AUT
[Ref.: #121199]	Continent	Europe
[Ref.: #121199]	Isolation date	2000
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Muridae (Mouse/Rat)
#Host Body-Site	#Organ	#Liver

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ537574 (>99% sequence identity) for Brachybacterium muris subclade from Microbeatlas

Aquatic Samples	730
Soil Samples	300
Animal Samples	6075
Plant Samples	146
Total Samples	7251

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5937]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121199]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5937]	Brachybacterium sp. C3H-21 partial 16S rRNA gene, strain C3H-21	AJ537574	1429	ENA	219301 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brachybacterium muris DSM 15460	GCA_016907455	contig	GenBank	219301 tax ID	*
[Ref.: #66792]	Brachybacterium muris strain DSM 15460	219301.5	wgs	PATRIC	219301 tax ID	*
[Ref.: #66792]	Brachybacterium muris DSM 15460	2893575648	draft	JGI IMG/M	219301 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brachybacterium muris strain DSM 15460 PATRIC: 219301.5
[Ref.: #69481]	spore-forming	no	85	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brachybacterium muris strain DSM 15460 PATRIC: 219301.5
[Ref.: #69480]	motile	no	94.232	no
[Ref.: #69480]	gram-positive	yes	92.251	no
[Ref.: #69480]	anaerobic	no	99.124	no
[Ref.: #69480]	aerobic	yes	82.34	no
[Ref.: #69480]	halophile	no	61.286	no
[Ref.: #69480]	spore-forming	no	93.962	no
[Ref.: #69480]	glucose-util	yes	88.857	no
[Ref.: #69480]	flagellated	no	97.884	no
[Ref.: #69480]	thermophile	no	98.575	yes
[Ref.: #69480]	glucose-ferment	no	71.579	no

Availability in culture collections External links

[Ref.: #5937]

Culture collection no.

DSM 15460, CCM 7047, JCM 14117, CCUG 50159, CCUG 50352, CIP 108210, DSM 14560

[Ref.: #73424]

SI-ID 127984

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft genome sequence of an Actinobacterium, Brachybacterium muris strain UCD-AY4.	Lo JR, Lang JM, Darling AE, Eisen JA, Coil DA	Genome Announc	10.1128/genomeA.00086-13	2013	*
Phylogeny	Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential.	Gontia I, Kavita K, Schmid M, Hartmann A, Jha B	Int J Syst Evol Microbiol	10.1099/ijs.0.023176-0	2011	*
Phylogeny	Brachybacterium phenoliresistens sp. nov., isolated from oil-contaminated coastal sand.	Chou JH, Lin KY, Lin MC, Sheu SY, Wei YH, Arun AB, Young CC, Chen WM	Int J Syst Evol Microbiol	10.1099/ijs.0.65019-0	2007	*
Phylogeny	Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain.	Buczolits S, Schumann P, Weidler G, Radax C, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.02728-0	2003	*

References

#5937

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15460

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33912 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#73424

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID127984.1 )

#121199 Collection of Institut Pasteur ; Curators of the CIP; CIP 108210
* These data were automatically processed and therefore are not curated

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Brachybacterium muris strain DSM 15460

Brachybacterium muris strain DSM 15460

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