Name: Brachybacterium sacelli R-5647
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 3913

Type strain:

Species: Brachybacterium sacelli

Strain Designation: R-5647

Culture col. no.: DSM 14566, LMG 20354, CCUG 50347, JCM 12139, CIP 108249, LMG 20345

Strain history: CIP <- 2004, JCM <- 2003, LMG <- J. Heyrman: strain R-5647

NCBI tax ID(s): 173364 (species)

SI-ID 84742

LMG 20345, DSM 14566, JCM 12139, CCUG 50347, CIP 108249

Section

Brachybacterium sacelli R-5647 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from mural painting.

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Dermabacteraceae*
	Genus	*Brachybacterium*
	Species	**Brachybacterium sacelli**
	Full Scientific Name (LPSN)	Brachybacterium sacelli Heyrman et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #122342]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #122342]	Cell shape	coccus-shaped

[Ref.: #122342]	Motility	no

[Ref.: #18517]	Colony color	Oyster white (1013)
[Ref.: #18517]	Incubation period	10-14 days
[Ref.: #18517]	Cultivation medium used	ISP 2

[Ref.: #18517]	Colony color	Oyster white (1013)
[Ref.: #18517]	Incubation period	10-14 days
[Ref.: #18517]	Cultivation medium used	ISP 3

[Ref.: #18517]	Colony color	Beige (1001)
[Ref.: #18517]	Incubation period	10-14 days
[Ref.: #18517]	Cultivation medium used	ISP 4

[Ref.: #18517]	Colony color	Beige (1001)
[Ref.: #18517]	Incubation period	10-14 days
[Ref.: #18517]	Cultivation medium used	ISP 5

[Ref.: #18517]	Colony color	Oyster white (1013)
[Ref.: #18517]	Incubation period	10-14 days
[Ref.: #18517]	Cultivation medium used	ISP 6

[Ref.: #18517]	Colony color	Beige (1001)
[Ref.: #18517]	Incubation period	10-14 days
[Ref.: #18517]	Cultivation medium used	ISP 7

[Ref.: #18517]	Multicellular complex forming ability	no
[Ref.: #18517]	Medium Name (multicellularity)	ISP 2

[Ref.: #18517]	Multicellular complex forming ability	no
[Ref.: #18517]	Medium Name (multicellularity)	ISP 3

[Ref.: #18517]	Multicellular complex forming ability	no
[Ref.: #18517]	Medium Name (multicellularity)	ISP 4

[Ref.: #18517]	Multicellular complex forming ability	no
[Ref.: #18517]	Medium Name (multicellularity)	ISP 5

[Ref.: #18517]	Multicellular complex forming ability	no
[Ref.: #18517]	Medium Name (multicellularity)	ISP 6

[Ref.: #18517]	Multicellular complex forming ability	no
[Ref.: #18517]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5388]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #5388]

Culture medium growth

[Ref.: #5388]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5388]

Culture medium composition

expand / minimize

[Ref.: #18517]

Culture medium

ISP 2

[Ref.: #18517]

Culture medium growth

[Ref.: #18517]

Culture medium composition

expand / minimize

[Ref.: #18517]

Culture medium

ISP 3

[Ref.: #18517]

Culture medium growth

[Ref.: #18517]

Culture medium composition

expand / minimize

[Ref.: #18517]

Culture medium

ISP 4

[Ref.: #18517]

Culture medium growth

[Ref.: #18517]

Culture medium composition

expand / minimize

[Ref.: #18517]

Culture medium

ISP 5

[Ref.: #18517]

Culture medium growth

[Ref.: #18517]

Culture medium composition

expand / minimize

[Ref.: #18517]

Culture medium

ISP 6

[Ref.: #18517]

Culture medium growth

[Ref.: #18517]

Culture medium composition

expand / minimize

[Ref.: #18517]

Culture medium

ISP 7

[Ref.: #18517]

Culture medium growth

[Ref.: #18517]

Culture medium composition

expand / minimize

[Ref.: #33217]

Culture medium

Marine agar (MA)

[Ref.: #33217]

Culture medium growth

[Ref.: #33217]

Culture medium composition

expand / minimize

[Ref.: #122342]

Culture medium

CIP Medium 13

[Ref.: #122342]

Culture medium growth

[Ref.: #122342]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #5388]	growth	28 °C
[Ref.: #18517]	optimum	28 °C
[Ref.: #33217]	growth	30 °C
[Ref.: #58847]	growth	28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #5388]	Temperature range	mesophilic
[Ref.: #18517]	Temperature range	mesophilic
[Ref.: #33217]	Temperature range	mesophilic
[Ref.: #58847]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #58847]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.767

[Ref.: #5388]	Murein short key	A31.02
[Ref.: #5388]	Murein types	A4gamma m-Dpm-D-Glu₂; alpha-carboxyl group of D-Glu substituted by glycine

[Ref.: #67770]

Observation

quinones: MK-7, MK-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18517]	arabinose ChEBI	-
[Ref.: #18517]	cellulose ChEBI	-
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #18517]	fructose ChEBI	-
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18517]	glucose ChEBI	-
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #18517]	mannitol ChEBI	+
[Ref.: #18517]	myo-inositol ChEBI	-
[Ref.: #122342]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #122342]	nitrite ChEBI	-	reduction
[Ref.: #18517]	raffinose ChEBI	-
[Ref.: #18517]	rhamnose ChEBI	-
[Ref.: #18517]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #18517]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #122342]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne
[Ref.: #122342]	catalase	+	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne
[Ref.: #122342]	oxidase	-
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122342]	urease	-	3.5.1.5
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18517]	151	-	-	+	-	-	+	+	+	+	+	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18517]	210	not determinedn.d.	-	+	+	-	+	+	+	-	-	-	-	+	+	+	+	+	+	-	+
[Ref.: #122342]	11023	-	+	+	+	+	+	-	-	-	-	-	+	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5388]	Sample type/isolated from	mural painting
[Ref.: #5388]	Geographic location (country and/or sea, region)	Herberstein, St. Katharinen Kapelle
[Ref.: #5388]	Country	Austria
[Ref.: #5388]	Country ISO 3 Code	AUT
[Ref.: #5388]	Continent	Europe

[Ref.: #58847]	Sample type/isolated from	Mural painting
[Ref.: #58847]	Geographic location (country and/or sea, region)	Castle Herberstein
[Ref.: #58847]	Country	Austria
[Ref.: #58847]	Country ISO 3 Code	AUT
[Ref.: #58847]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Microbial biofilm damaging the mural painting at the Saint-Catherine chapel of Castle Herberstein
[Ref.: #67770]	Country	Austria
[Ref.: #67770]	Country ISO 3 Code	AUT
[Ref.: #67770]	Continent	Europe

[Ref.: #122342]	Sample type/isolated from	Microbial biofilm damaging the mural painting
[Ref.: #122342]	Geographic location (country and/or sea, region)	Herberstein castle, Himsdorf
[Ref.: #122342]	Country	Austria
[Ref.: #122342]	Country ISO 3 Code	AUT
[Ref.: #122342]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	-
#Engineered	#Other	#Painting

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ415384 (>99% sequence identity) for Brachybacterium sacelli from Microbeatlas

Aquatic Samples	38
Soil Samples	45
Animal Samples	480
Plant Samples	15
Total Samples	578

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18517]	Biosafety level	1	Risk group
[Ref.: #5388]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122342]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5388]	Brachybacterium sacelli partial 16S rRNA gene, type strain LMG 20345T	AJ415384	1405	ENA	173364 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brachybacterium sacelli DSM 14566	GCA_017876545	contig	GenBank	173364 tax ID	*
[Ref.: #66792]	Brachybacterium sacelli strain DSM 14566	173364.3	wgs	PATRIC	173364 tax ID	*
[Ref.: #66792]	Brachybacterium sacelli DSM 14566	2918362552	draft	JGI IMG/M	173364 tax ID	*

[Ref.: #5388]

GC-content

70.3 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brachybacterium sacelli strain DSM 14566 PATRIC: 173364.3
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brachybacterium sacelli strain DSM 14566 PATRIC: 173364.3
[Ref.: #69480]	motile	no	89.518	no
[Ref.: #69480]	gram-positive	yes	92.139	no
[Ref.: #69480]	anaerobic	no	99.583	yes
[Ref.: #69480]	aerobic	yes	90.589	yes
[Ref.: #69480]	halophile	yes	69.095	no
[Ref.: #69480]	spore-forming	no	88.078	no
[Ref.: #69480]	glucose-util	yes	89.638	no
[Ref.: #69480]	flagellated	no	97.333	no
[Ref.: #69480]	thermophile	no	99.358	yes
[Ref.: #69480]	glucose-ferment	no	64.616	no

Availability in culture collections External links

[Ref.: #5388]

Culture collection no.

DSM 14566, LMG 20354, CCUG 50347, JCM 12139, CIP 108249, LMG 20345

[Ref.: #73423]

SI-ID 84742

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brachybacterium hainanense sp. nov., isolated from noni (Morinda citrifolia L.) branch.	Liu Y, Zhai L, Yao S, Cao Y, Cao Y, Zhang X, Su J, Ge Y, Zhao R, Cheng C	Int J Syst Evol Microbiol	10.1099/ijsem.0.000559	2015	*
Phylogeny	Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential.	Gontia I, Kavita K, Schmid M, Hartmann A, Jha B	Int J Syst Evol Microbiol	10.1099/ijs.0.023176-0	2011	*
Phylogeny	Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain.	Buczolits S, Schumann P, Weidler G, Radax C, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.02728-0	2003	*
Phylogeny	Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria).	Heyrman J, Balcaen A, De Vos P, Schumann P, Swings J	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1641	2002	*

References

#5388

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14566

#18517

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33217 ; Curators of the CIP;
#58847 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 50347

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#73423

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID84742.1 )

#122342 Collection of Institut Pasteur ; Curators of the CIP; CIP 108249
* These data were automatically processed and therefore are not curated

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Brachybacterium sacelli strain DSM 14566

Brachybacterium sacelli strain DSM 14566

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