Brachybacterium sacelli (DSM 14566, LMG 20354, CCUG 50347)

Name: Brachybacterium sacelli (DSM 14566, LMG 20354, CCUG 50347)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 3913

Type strain

Strain Designation R-5647

Culture col. no. DSM 14566 LMG 20354 CCUG 50347 R-5647 LMG 20345 2 more

NCBI tax ID(s) 173364

Special Collections DSMZ CCUG JCM CIP Wink compendium

History <- J. Heyrman; R-5647 LMG 20345 <-- J. Heyrman R-5647. CIP <- 2004, JCM <- 2003, LMG <- J. Heyrman: strain R-5647

Links

Brachybacterium sacelli R-5647 is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from mural painting.

Gram-positive coccus-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 84742

LMG 20345,DSM 14566,JCM 12139,CCUG 50347,CIP 108249

@ref 20215

Last LPSN update 2026-02-09 11:18:36

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Dermabacteraceae

Genus Brachybacterium

Species Brachybacterium sacelli

Full scientific name Brachybacterium sacelli Heyrman et al. 2002

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
122342	positive	coccus-shaped
125438			90
125439	positive		98.4

Colony morphology

@ref	Colony color	Incubation period	Medium used
18517	Oyster white (1013)	10-14 days	ISP 2
18517	Oyster white (1013)	10-14 days	ISP 3
18517	Beige (1001)	10-14 days	ISP 4
18517	Beige (1001)	10-14 days	ISP 5
18517	Oyster white (1013)	10-14 days	ISP 6
18517	Beige (1001)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18517		ISP 2
18517		ISP 3
18517		ISP 4
18517		ISP 5
18517		ISP 6
18517		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5388	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
18517	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18517	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18517	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18517	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18517	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18517	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33217	Marine agar (MA)		Distilled water make up to (1000.000 ml);Marine agar (55.100 g)
122342	CIP Medium 13	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5388	positive	growth	28
18517	positive	optimum	28
33217	positive	growth	30
58847	positive	growth	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

58847

Oxygen toleranceaerobe

Murein

@ref	Murein short key	Type
5388	A31.02	A4gamma m-Dpm-D-Glu₂; alpha-carboxyl group of D-Glu substituted by glycine

Observation

67770

Observationquinones: MK-7, MK-8

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
18517	22599 ChEBI	arabinose	-
18517	62968 ChEBI	cellulose	-
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
18517	28757 ChEBI	fructose	-
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
18517	17234 ChEBI	glucose	-
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
18517	29864 ChEBI	mannitol	+
18517	17268 ChEBI	myo-inositol	-
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
122342	17632 ChEBI	nitrate	+	reduction
122342	16301 ChEBI	nitrite	-	reduction
18517	16634 ChEBI	raffinose	-
18517	26546 ChEBI	rhamnose	-
18517	17992 ChEBI	sucrose	-
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
18517	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122342	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
122342	catalase	+	1.11.1.6
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
122342	oxidase	-
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
122342	urease	-	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18517	-	-	+	-	-	+	+	+	+	+	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18517	not determinedn.d.	-	+	+	-	+	+	+	-	-	-	-	+	+	+	+	+	+	-	+
122342	-	+	+	+	+	+	-	-	-	-	-	+	-	+	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	-
#Engineered	#Other	#Painting

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
5388	mural painting	Herberstein, St. Katharinen Kapelle	Austria	AUT	Europe
58847	Mural painting	Castle Herberstein	Austria	AUT	Europe
67770	Microbial biofilm damaging the mural painting at the Saint-Catherine chapel of Castle Herberstein		Austria	AUT	Europe
122342	Microbial biofilm damaging the mural painting	Herberstein castle, Himsdorf	Austria	AUT	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ415384 (>99% sequence identity) for Brachybacterium sacelli from Microbeatlas

Aquatic Samples	38
Soil Samples	45
Animal Samples	480
Plant Samples	15
Total Samples	578

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
18517	1	Risk group According to TRBA 466
5388	1	Risk group (German classification) According to TRBA 466
122342	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1787654v1 assembly for Brachybacterium sacelli DSM 14566	contig	GCA_017876545	173364.3	2918362552	173364	66.19
124043	ASM4265526v1 assembly for Brachybacterium sacelli CCUG 50347	contig	GCA_042655265			173364	37.73
124043	ASM4265776v1 assembly for Brachybacterium sacelli JCM 12139	contig	GCA_042657765			173364	37.68
124043	ASM3952738v1 assembly for Brachybacterium sacelli JCM 12139	contig	GCA_039527385			173364	35.73

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5388	Brachybacterium sacelli partial 16S rRNA gene, type strain LMG 20345T	AJ415384	1405		173364

GC content

@ref	GC-content (mol%)	Method
5388	70.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brachybacterium sacelli DSM 14566
NCBI: GCA_017876545

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	67.60	no
125439	motility	BacteriaNetⓘ	no	79.10	no
125439	gram_stain	BacteriaNetⓘ	positive	98.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	78.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Brachybacterium sacelli strain DSM 14566
PATRIC: 173364.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.96	no
125438	anaerobic	anaerobicⓘ	no	96.96	no
125438	spore-forming	spore-formingⓘ	no	68.84	no
125438	aerobic	aerobicⓘ	yes	78.38	yes
125438	thermophilic	thermophileⓘ	no	95.92	yes
125438	flagellated	motile2+ⓘ	no	90.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Selective isolation and genomic characterization of biopolymer producer-a novel feature of halophile Brachybacterium paraconglomeratum MTCC 13074.	Mandragutti T, Sudhakar G.	J Genet Eng Biotechnol	10.1186/s43141-023-00484-y	2023
Phylogeny	Brachybacterium endophyticum sp. nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.	Tuo L, Yan XR, Li FN, Bao YX, Shi HC, Li HY, Sun CH.	Int J Syst Evol Microbiol	10.1099/ijsem.0.003032	2018
Phylogeny	Brachybacterium hainanense sp. nov., isolated from noni (Morinda citrifolia L.) branch.	Liu Y, Zhai L, Yao S, Cao Y, Cao Y, Zhang X, Su J, Ge Y, Zhao R, Cheng C	Int J Syst Evol Microbiol	10.1099/ijsem.0.000559	2015
Phylogeny	Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential.	Gontia I, Kavita K, Schmid M, Hartmann A, Jha B	Int J Syst Evol Microbiol	10.1099/ijs.0.023176-0	2011
Phylogeny	Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain.	Buczolits S, Schumann P, Weidler G, Radax C, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.02728-0	2003
Phylogeny	Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria).	Heyrman J, Balcaen A, De Vos P, Schumann P, Swings J	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1641	2002

References

#5388	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14566
#18517	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#33217	; Curators of the CIP;
#58847	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 50347
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122342	Collection of Institut Pasteur ; Curators of the CIP; CIP 108249
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brachybacterium sacelli (DSM 14566, LMG 20354, CCUG 50347)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Murein

Observation

Metabolite utilization

Metabolite production

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_017876545

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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