Name: Pusillimonas harenae B201
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 365

Type strain:

Species: Pusillimonas harenae

Strain Designation: B201

Culture col. no.: DSM 25667, JCM 16917, KACC 14927

Strain history: C. O. Jeon B201.

NCBI tax ID(s): 657015 (species)

SI-ID 402067

DSM 25667

Special Collections

Section

Pusillimonas harenae B201 is an aerobe, Gram-negative, motile bacterium that was isolated from tidal flat.

Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Alcaligenaceae*
	Genus	*Pusillimonas*
	Species	**Pusillimonas harenae**
	Full Scientific Name (LPSN)	Pusillimonas harenae Park et al. 2011

Synonym

Pollutimonas harenae

Information on morphological and physiological properties Morphology

[Ref.: #23279]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %94.939

[Ref.: #23279]	Cell length	0.6-0.9 µm

[Ref.: #23279]	Cell width	0.5-0.7 µm

[Ref.: #23279]	Cell shape	rod-shaped

[Ref.: #23279]	Motility	yes

[Ref.: #23279]	Flagellum arrangement	amphitrichous

[Ref.: #23279]	Colony color	ivory
[Ref.: #23279]	Colony shape	circular
[Ref.: #23279]	Cultivation medium used	R2A agar

[Ref.: #18083]	Incubation period	2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18083]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #18083]

Culture medium growth

[Ref.: #18083]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18083]

Culture medium composition

expand / minimize

[Ref.: #23279]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #23279]

Culture medium growth

		Temperature
[Ref.: #18083]	growth	28 °C
[Ref.: #23279]	growth	15-45 °C
[Ref.: #23279]	optimum	30 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #23279]			optimum	6.0-7.5
[Ref.: #23279]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23279]

Oxygen tolerance

aerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23279]		NaCl		maximum	6	%
[Ref.: #23279]		NaCl		optimum	0-3	%

[Ref.: #67770]

Observation

quinones: Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23279]	adipate ChEBI	+	assimilation
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23279]	citrate ChEBI	-	assimilation
[Ref.: #23279]	D-glucose ChEBI	-	assimilation
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #23279]	D-mannitol ChEBI	-	assimilation
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #23279]	D-mannose ChEBI	-	assimilation
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #23279]	decanoate ChEBI	-	assimilation
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #23279]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23279]	gelatin ChEBI	-	hydrolysis
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23279]	gluconate ChEBI	-	assimilation
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #23279]	glucose ChEBI	-	builds acid from
[Ref.: #23279]	L-arabinose ChEBI	-	assimilation
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #23279]	malate ChEBI	+	assimilation
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23279]	maltose ChEBI	-	assimilation
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #23279]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #23279]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #23279]	phenylacetate ChEBI	-	assimilation
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #23279]	urea ChEBI	+	hydrolysis
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #23279]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #23279]	acid phosphatase	+	3.1.3.2
[Ref.: #23279]	alkaline phosphatase	+/-	3.1.3.1
[Ref.: #23279]	alpha-chymotrypsin	+/-	3.4.21.1
[Ref.: #23279]	alpha-fucosidase	-	3.2.1.51
[Ref.: #23279]	alpha-galactosidase	-	3.2.1.22
[Ref.: #23279]	alpha-glucosidase	-	3.2.1.20
[Ref.: #23279]	alpha-mannosidase	-	3.2.1.24
[Ref.: #23279]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #23279]	beta-galactosidase	-	3.2.1.23
[Ref.: #23279]	beta-glucosidase	-	3.2.1.21
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #23279]	beta-glucuronidase	-	3.2.1.31
[Ref.: #23279]	catalase	+	1.11.1.6
[Ref.: #23279]	cystine arylamidase	+	3.4.11.3
[Ref.: #23279]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #23279]	esterase (C 4)	+
[Ref.: #23279]	esterase lipase (C 8)	+
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #23279]	leucine arylamidase	+	3.4.11.1
[Ref.: #23279]	lipase (C 14)	-
[Ref.: #23279]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #23279]	naphthol-AS-BI-phosphohydrolase	+/-
[Ref.: #23279]	trypsin	+/-	3.4.21.4
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #23279]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #18083]	24692	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18083]	Sample type/isolated from	tidal flat
[Ref.: #18083]	Geographic location (country and/or sea, region)	Taean coast
[Ref.: #18083]	Country	Republic of Korea
[Ref.: #18083]	Country ISO 3 Code	KOR
[Ref.: #18083]	Continent	Asia

[Ref.: #23279]	Sample type/isolated from	tidal flat
[Ref.: #23279]	Geographic location (country and/or sea, region)	Taean coast
[Ref.: #23279]	Geographic location	36.8347°/126.1520°

[Ref.: #67770]	Sample type/isolated from	Beach sand from the Taean coast
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #23279]	Isolation/enrichment procedure	soil sample was serially diluted in 0.9 % (w/v) saline and spread on marine agar 2216 (MA; Difco) plates and incubated under aerobic conditions at 25 uC for 5 days
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Tidal flat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GQ232740 (>99% sequence identity) for Pusillimonas from Microbeatlas

Aquatic Samples	14
Soil Samples	61
Animal Samples	94
Plant Samples	1
Total Samples	170

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18083]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #18083]	Pusillimonas harenae strain B201 16S ribosomal RNA gene, partial sequence	GQ232740	1400	GenBank ENA	657015 tax ID
[Ref.: #67770]	Pusillimonas harenae JCM 16917 gene for 16S ribosomal RNA, partial sequence	LC508806	1463	GenBank ENA	657015 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pollutimonas harenae JCM 16917	GCA_004153685	contig	GenBank ENA	657015 tax ID
[Ref.: #66792]	Pollutimonas harenae DSM 25667	GCA_013416515	contig	GenBank ENA	657015 tax ID	*
[Ref.: #66792]	Pusillimonas harenae strain DSM 25667	657015.5	wgs	PATRIC	657015 tax ID	*
[Ref.: #66792]	Pusillimonas harenae strain JCM 16917	657015.4	wgs	PATRIC	657015 tax ID	*
[Ref.: #66792]	Pusillimonas harenae JCM 16917	2891687248	draft	JGI IMG/M	657015 tax ID	*

[Ref.: #23279]

GC-content

53.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pusillimonas harenae strain JCM 16917 PATRIC: 657015.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pusillimonas harenae strain DSM 25667 PATRIC: 657015.5
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	94.939	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.148	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	86.267	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	82.83	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.432	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	84.675	yes

Availability in culture collections External links

[Ref.: #18083]

Culture collection no.

DSM 25667, JCM 16917, KACC 14927

[Ref.: #70045]

SI-ID 402067

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pusillimonas harenae sp. nov., isolated from a sandy beach, and emended description of the genus Pusillimonas.	Park MS, Park YJ, Jung JY, Lee SH, Park W, Lee K, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijs.0.029892-0	2011	*

References

#18083

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25667

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23279

Moon Su Park, Youn-Je Park, Ji Young Jung, Seung Hyeon Lee, Woojun Park, Kangseok Lee, Che Ok Jeon: Pusillimonas harenae sp. nov., isolated from a sandy beach, and emended description of the genus Pusillimonas. IJSEM 61: 2901 - 2906 2011 ( DOI 10.1099/ijs.0.029892-0 , PubMed 21257696 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70045

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402067.1 )

* These data were automatically processed and therefore are not curated

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Pusillimonas harenae strain JCM 16917

Pusillimonas harenae strain DSM 25667

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