Name: Pusillimonas soli MJ07
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 363

Type strain:

Species: Pusillimonas soli

Strain Designation: MJ07

Culture col. no.: DSM 25264, JCM 16386, KCTC 22455

Strain history: <- MJ Lee, H-Plus Eco, Korea

NCBI tax ID(s): 659016 (species)

SI-ID 404651

DSM 25264

Section

Pusillimonas soli MJ07 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from farm soil.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Alcaligenaceae*
	Genus	*Pusillimonas*
	Species	**Pusillimonas soli**
	Full Scientific Name (LPSN)	Pusillimonas soli Lee et al. 2010

Synonym

Allopusillimonas soli

Information on morphological and physiological properties Morphology

[Ref.: #23277]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.994

[Ref.: #23277]	Cell length	0.7-1.0 µm

[Ref.: #23277]	Cell width	0.3-0.5 µm

[Ref.: #23277]	Cell shape	rod-shaped

[Ref.: #23277]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %94.589

Multimedia content

[Ref.: #17962]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17962]

Culture medium

LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)

[Ref.: #17962]

Culture medium growth

[Ref.: #17962]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17962]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17962]	growth	28 °C
[Ref.: #23277]	optimum	30 °C
[Ref.: #67770]	growth	30 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #17962]	Temperature range	mesophilic
[Ref.: #23277]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23277]			optimum	6.5-7.0
[Ref.: #23277]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

[Ref.: #23277]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.994

[Ref.: #67770]

Observation

quinones: Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23277]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23277]	2-dehydro-D-gluconate ChEBI	-	growth
[Ref.: #23277]	3-hydroxybenzoate ChEBI	+	assimilation
[Ref.: #23277]	3-hydroxybutyrate ChEBI	+	growth
[Ref.: #23277]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23277]	5-dehydro-D-gluconate ChEBI	-	growth
[Ref.: #23277]	acetate ChEBI	+	growth
[Ref.: #23277]	adipate ChEBI	+	assimilation
[Ref.: #23277]	amygdalin ChEBI	-	builds acid from
[Ref.: #23277]	arbutin ChEBI	+	builds acid from
[Ref.: #23277]	casein	-	hydrolysis
[Ref.: #23277]	cellobiose ChEBI	-	builds acid from
[Ref.: #23277]	chitin ChEBI	-	hydrolysis
[Ref.: #23277]	citrate ChEBI	+	assimilation
[Ref.: #23277]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23277]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23277]	D-fructose ChEBI	-	builds acid from
[Ref.: #23277]	D-fucose ChEBI	-	builds acid from
[Ref.: #23277]	D-galactose ChEBI	-	builds acid from
[Ref.: #23277]	D-gluconate ChEBI	+	builds acid from
[Ref.: #23277]	D-glucose ChEBI	-	growth
[Ref.: #23277]	D-glucose ChEBI	+	builds acid from
[Ref.: #23277]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23277]	D-mannitol ChEBI	-	builds acid from
[Ref.: #23277]	D-mannitol ChEBI	-	growth
[Ref.: #23277]	D-mannose ChEBI	-	builds acid from
[Ref.: #23277]	D-mannose ChEBI	-	growth
[Ref.: #23277]	D-ribose ChEBI	+	assimilation
[Ref.: #23277]	D-ribose ChEBI	+	builds acid from
[Ref.: #23277]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #23277]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #23277]	D-tagatose ChEBI	-	builds acid from
[Ref.: #23277]	D-xylose ChEBI	-	builds acid from
[Ref.: #23277]	decanoate ChEBI	-	assimilation
[Ref.: #23277]	dna ChEBI	-	hydrolysis
[Ref.: #23277]	erythritol ChEBI	-	builds acid from
[Ref.: #23277]	esculin ChEBI	-	builds acid from
[Ref.: #23277]	galactitol ChEBI	-	builds acid from
[Ref.: #23277]	gentiobiose ChEBI	-	builds acid from
[Ref.: #23277]	gluconate ChEBI	-	growth
[Ref.: #23277]	glycerol ChEBI	+	builds acid from
[Ref.: #23277]	glycogen ChEBI	-	builds acid from
[Ref.: #23277]	glycogen ChEBI	-	growth
[Ref.: #23277]	hydroxyethylcellulose ChEBI	-	hydrolysis
[Ref.: #23277]	inulin ChEBI	-	builds acid from
[Ref.: #23277]	itaconate ChEBI	+	assimilation
[Ref.: #23277]	L-alanine ChEBI	+	assimilation
[Ref.: #23277]	L-arabinose ChEBI	-	growth
[Ref.: #23277]	L-arabinose ChEBI	+	builds acid from
[Ref.: #23277]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23277]	L-fucose ChEBI	-	builds acid from
[Ref.: #23277]	L-fucose ChEBI	-	growth
[Ref.: #23277]	L-histidine ChEBI	+	assimilation
[Ref.: #23277]	L-proline ChEBI	+	assimilation
[Ref.: #23277]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23277]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #23277]	L-serine ChEBI	+	assimilation
[Ref.: #23277]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23277]	L-xylose ChEBI	-	builds acid from
[Ref.: #23277]	lactate ChEBI	+	growth
[Ref.: #23277]	lactose ChEBI	-	builds acid from
[Ref.: #23277]	malate ChEBI	+	assimilation
[Ref.: #23277]	malonate ChEBI	+	assimilation
[Ref.: #23277]	maltose ChEBI	-	builds acid from
[Ref.: #23277]	maltose ChEBI	-	growth
[Ref.: #23277]	melezitose ChEBI	-	builds acid from
[Ref.: #23277]	melibiose ChEBI	-	builds acid from
[Ref.: #23277]	melibiose ChEBI	-	growth
[Ref.: #23277]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #23277]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #23277]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from
[Ref.: #23277]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23277]	myo-inositol ChEBI	+	assimilation
[Ref.: #23277]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #23277]	N-acetylglucosamine ChEBI	-	growth
[Ref.: #23277]	nitrate ChEBI	+	reduction
[Ref.: #23277]	phenylacetate ChEBI	+	assimilation
[Ref.: #23277]	propionate ChEBI	+	assimilation
[Ref.: #23277]	raffinose ChEBI	-	builds acid from
[Ref.: #23277]	ribitol ChEBI	-	builds acid from
[Ref.: #23277]	salicin ChEBI	-	builds acid from
[Ref.: #23277]	salicin ChEBI	-	growth
[Ref.: #23277]	starch ChEBI	-	builds acid from
[Ref.: #23277]	starch ChEBI	-	hydrolysis
[Ref.: #23277]	suberic acid ChEBI	+	assimilation
[Ref.: #23277]	sucrose ChEBI	-	builds acid from
[Ref.: #23277]	sucrose ChEBI	-	growth
[Ref.: #23277]	trehalose ChEBI	-	builds acid from
[Ref.: #23277]	turanose ChEBI	-	builds acid from
[Ref.: #23277]	valerate ChEBI	+	assimilation
[Ref.: #23277]	xylan ChEBI	-	hydrolysis
[Ref.: #23277]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #23277]	Acid phosphatase	+	3.1.3.2
[Ref.: #23277]	Alkaline phosphatase	+	3.1.3.1
[Ref.: #23277]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #23277]	alpha-fucosidase	-	3.2.1.51
[Ref.: #23277]	alpha-galactosidase	-	3.2.1.22
[Ref.: #23277]	alpha-glucosidase	-	3.2.1.20
[Ref.: #23277]	alpha-mannosidase	-	3.2.1.24
[Ref.: #23277]	beta-galactosidase	-	3.2.1.23
[Ref.: #23277]	beta-glucosidase	-	3.2.1.21
[Ref.: #23277]	beta-glucuronidase	-	3.2.1.31
[Ref.: #23277]	catalase	+	1.11.1.6
[Ref.: #23277]	cystine arylamidase	-	3.4.11.3
[Ref.: #23277]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23277]	esterase (C 4)	+
[Ref.: #23277]	esterase lipase (C 8)	+
[Ref.: #23277]	leucine arylamidase	+	3.4.11.1
[Ref.: #23277]	lipase (C 14)	-
[Ref.: #23277]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #23277]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #23277]	trypsin	-	3.4.21.4
[Ref.: #23277]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17962]	Sample type/isolated from	farm soil
[Ref.: #17962]	Geographic location (country and/or sea, region)	near Daejeon
[Ref.: #17962]	Country	Republic of Korea
[Ref.: #17962]	Country ISO 3 Code	KOR
[Ref.: #17962]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Farm soil near Daejeon
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	#Field
#Engineered	#Food production	#Aquaculture

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17962]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17962]	Pusillimonas soli strain MJ07 16S ribosomal RNA gene, partial sequence	GQ241322	1454	ENA	659016 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Allopusillimonas soli KCTC 22455	GCA_004153445	scaffold	GenBank	659016 tax ID
[Ref.: #66792]	Allopusillimonas soli DSM 25264	GCA_013416335	contig	GenBank	659016 tax ID	*
[Ref.: #66792]	Pusillimonas soli strain DSM 25264	659016.5	wgs	PATRIC	659016 tax ID	*
[Ref.: #66792]	Pusillimonas soli strain KCTC 22455	659016.4	wgs	PATRIC	659016 tax ID	*
[Ref.: #66792]	Pusillimonas soli KCTC 22455	2891629496	draft	JGI IMG/M	659016 tax ID	*

[Ref.: #17962]	GC-content	59.4 mol%
[Ref.: #23277]	GC-content	59.4 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pusillimonas soli KCTC 22455 NCBI: GCA_004153445.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pusillimonas soli strain DSM 25264 PATRIC: 659016.5
[Ref.: #69480]	gram-positive	no	98.039	yes
[Ref.: #69480]	anaerobic	no	99.246	yes
[Ref.: #69480]	halophile	no	74.962	no
[Ref.: #69480]	spore-forming	no	94.162	yes
[Ref.: #69480]	glucose-util	no	76.983	yes
[Ref.: #69480]	thermophile	no	96.73	yes
[Ref.: #69480]	flagellated	no	73.271	no
[Ref.: #69480]	motile	yes	86.672	yes
[Ref.: #69480]	aerobic	yes	90.419	no
[Ref.: #69480]	glucose-ferment	no	91.875	no

Availability in culture collections External links

[Ref.: #17962]

Culture collection no.

DSM 25264, JCM 16386, KCTC 22455

[Ref.: #70043]

SI-ID 404651

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pusillimonas soli sp. nov., isolated from farm soil.	Lee M, Woo SG, Chae M, Ten LN	Int J Syst Evol Microbiol	10.1099/ijs.0.020404-0	2009	*

References

#17962

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25264

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23277

Myungjin Lee, Sung-Geun Woo, Myoungsoo Chae, Leonid N. Ten: Pusillimonas soli sp. nov., isolated from farm soil. IJSEM 60: 2326 - 2330 2010 ( DOI 10.1099/ijs.0.020404-0 , PubMed 19933592 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70043

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404651.1 )

* These data were automatically processed and therefore are not curated

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Pusillimonas soli KCTC 22455

Pusillimonas soli strain DSM 25264

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