Name: Castellaniella denitrificans NKNTAU
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 348

Type strain:

Species: Castellaniella denitrificans

Strain Designation: NKNTAU

Culture col. no.: CIP 109221, DSM 11046, CCUG 39541

Strain history: CIP <- 2006, CCUG <- 1998, K. Denger, Konstanz Univ., Konstanz, Germany: strain NKNTAU

NCBI tax ID(s): 56119 (species)

SI-ID 359321

CCUG 39541, DSM 11046, CIP 109221

Section

Castellaniella denitrificans NKNTAU is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from anaerobic sewage digester.

Gram-negative
motile
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Alcaligenaceae*
	Genus	*Castellaniella*
	Species	**Castellaniella denitrificans**
	Full Scientific Name (LPSN)	Castellaniella denitrificans Kämpfer et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31661]	Gram stain	negative
[Ref.: #118893]	Gram stain	negative

[Ref.: #31661]	Cell length	1.3-2 µm

[Ref.: #31661]	Cell width	0.2-0.8 µm

[Ref.: #31661]	Cell shape	rod-shaped
[Ref.: #118893]	Cell shape	rod-shaped

[Ref.: #31661]	Motility	yes
[Ref.: #118893]	Motility	yes

[Ref.: #31661]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4168]

Culture medium

ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503)

[Ref.: #4168]

Culture medium growth

[Ref.: #4168]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4168]

Culture medium composition

expand / minimize

[Ref.: #37484]

Culture medium

MEDIUM 328- for nutrient agar

[Ref.: #37484]

Culture medium growth

[Ref.: #37484]

Culture medium composition

expand / minimize

[Ref.: #118893]

Culture medium

CIP Medium 328

[Ref.: #118893]

Culture medium growth

[Ref.: #118893]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #4168]	growth	30 °C
[Ref.: #31661]	growth	25-42 °C
[Ref.: #31661]	optimum	25-30 °C
[Ref.: #37484]	growth	30 °C
[Ref.: #54834]	growth	30-37 °C

[Ref.: #4168]	Temperature range	mesophilic
[Ref.: #31661]	Temperature range	mesophilic
[Ref.: #37484]	Temperature range	mesophilic
[Ref.: #54834]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31661]			optimum	7
[Ref.: #31661]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4168]	Oxygen tolerance	anaerobe
[Ref.: #31661]	Oxygen tolerance	facultative anaerobe
[Ref.: #54834]	Oxygen tolerance	aerobe
[Ref.: #118893]	Oxygen tolerance	obligate aerobe

[Ref.: #31661]

Ability of spore formation

[Ref.: #31661]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31661]	acetate ChEBI	+	carbon source
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #31661]	alanine ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #31661]	aspartate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #31661]	gluconate ChEBI	+	carbon source
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #31661]	lactate ChEBI	+	carbon source
[Ref.: #31661]	leucine ChEBI	+	carbon source
[Ref.: #31661]	malate ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #118893]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #118893]	nitrite ChEBI	+	reduction
[Ref.: #31661]	ornithine ChEBI	+	carbon source
[Ref.: #31661]	proline ChEBI	+	carbon source
[Ref.: #31661]	propionate ChEBI	+	carbon source
[Ref.: #31661]	pyruvate ChEBI	+	carbon source
[Ref.: #31661]	serine ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #118893]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118893]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #118893]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118893]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #54834]	7911	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #54834]	4039	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #118893]	11727	-	+	+	-	-	+	-	-	-	-	-	-	+	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4168]	Sample type/isolated from	anaerobic sewage digester
[Ref.: #4168]	Country	Germany
[Ref.: #4168]	Country ISO 3 Code	DEU
[Ref.: #4168]	Continent	Europe

[Ref.: #54834]	Sample type/isolated from	Anaerobic sewage digester
[Ref.: #54834]	Country	Germany
[Ref.: #54834]	Country ISO 3 Code	DEU
[Ref.: #54834]	Continent	Europe

[Ref.: #118893]	Sample type/isolated from	Environment, Anaerobic sewage digester
[Ref.: #118893]	Country	Germany
[Ref.: #118893]	Country ISO 3 Code	DEU
[Ref.: #118893]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Wastewater
#Condition	#Anoxic (anaerobic)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence U82826 (>99% sequence identity) for Castellaniella denitrificans from Microbeatlas

Aquatic Samples	3384
Soil Samples	1695
Animal Samples	1188
Plant Samples	553
Total Samples	6820

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4168]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118893]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4168]	Castellaniella denitrificans strain NKNTAU 16S ribosomal RNA gene, complete sequence	U82826	1470	ENA	56119 tax ID

Availability in culture collections External links

[Ref.: #4168]

Culture collection no.

CIP 109221, DSM 11046, CCUG 39541

[Ref.: #70028]

SI-ID 359321

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Castellaniella hirudinis sp. nov., isolated from the skin of Hirudo verbana.	Glaeser SP, Galatis H, Martin K, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.041392-0	2012	*
Phylogeny	Castellaniella daejeonensis sp. nov., isolated from soil.	Lee M, Jung HM, Woo SG, Yoo SA, Ten LN	Int J Syst Evol Microbiol	10.1099/ijs.0.018887-0	2009	*
Phylogeny	Castellaniella ginsengisoli sp. nov., a beta-glucosidase-producing bacterium.	Kim MK, Srinivasan S, Kim YJ, Yang DC	Int J Syst Evol Microbiol	10.1099/ijs.0.008300-0	2009	*
Phylogeny	Castellaniella caeni sp. nov., a denitrifying bacterium isolated from sludge of a leachate treatment plant.	Liu QM, Ten LN, Im WT, Lee ST	Int J Syst Evol Microbiol	10.1099/ijs.0.65314-0	2008	*
Phylogeny	Castellaniella gen. nov., to accommodate the phylogenetic lineage of Alcaligenes defragrans, and proposal of Castellaniella defragrans gen. nov., comb. nov. and Castellaniella denitrificans sp. nov.	Kampfer P, Denger K, Cook AM, Lee ST, Jackel U, Denner EBM, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63989-0	2006	*

References

#4168

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11046

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31661

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27942 (see below)

#37484 ; Curators of the CIP;
#54834 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 39541

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#70028

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID359321.1 )

#118893 Collection of Institut Pasteur ; Curators of the CIP; CIP 109221

#27942

IJSEM 815 2006 ( DOI 10.1099/ijs.0.63989-0 , PubMed 16585701 )

* These data were automatically processed and therefore are not curated

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