Castellaniella denitrificans (CIP 109221, DSM 11046, CCUG 39541)

Name: Castellaniella denitrificans (CIP 109221, DSM 11046, CCUG 39541)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 348

Type strain

Strain Designation NKNTAU

Culture col. no. CIP 109221 DSM 11046 CCUG 39541 NKNTAU

NCBI tax ID(s) 56119

Special Collections DSMZ CCUG CIP Literature strains

History <- K. Denger, Universität Konstanz, Fakultät für Biologie, Konstanz, Germany; NKNTAU (Alcaligenes sp.) <- K. Denger {1996} CIP <- 2006, CCUG <- 1998, K. Denger, Konstanz Univ., Konstanz, Germany: strain NKNTAU

Links

Castellaniella denitrificans NKNTAU is an anaerobe, Gram-negative, motile bacterium that was isolated from anaerobic sewage digester.

Gram-negative motile rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 359321

CCUG 39541,DSM 11046,CIP 109221

@ref 20215

Last LPSN update 2026-02-09 11:17:51

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Alcaligenaceae

Genus Castellaniella

Species Castellaniella denitrificans

Full scientific name Castellaniella denitrificans Kämpfer et al. 2006

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31661	negative	1.3-2 µm	0.2-0.8 µm	rod-shaped
118893	negative			rod-shaped

Colony morphology

Pigmentation

31661

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4168	ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503)	Medium recipe at MediaDive	Name: ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503; with strain-specific modifications) Composition: NaNO3 1.69492 g/l Na2CO3 1.49551 g/l Taurine 1.24626 g/l NaCl 0.997008 g/l KCl 0.498505 g/l MgCl2 x 6 H2O 0.398803 g/l Na2S x 9 H2O 0.299103 g/l NH4Cl 0.249252 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149552 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l Calcium pantothenate 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water
37484	MEDIUM 328- for nutrient agar		Distilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g)
118893	CIP Medium 328	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
4168	positive	growth	30
31661	positive	growth	25-42
31661	positive	optimum	25-30
37484	positive	growth	30
54834	positive	growth	30-37

Culture pH

@ref	Ability	Type	PH
31661	positive	optimum	7
31661	positive	growth	7

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
4168	anaerobe
31661	facultative anaerobe
54834	aerobe
118893	obligate aerobe

Spore formation

31661

Spore formationno

Observation

31661

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31661	30089 ChEBI	acetate	+	carbon source
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
31661	16449 ChEBI	alanine	+	carbon source
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
31661	35391 ChEBI	aspartate	+	carbon source
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
31661	24265 ChEBI	gluconate	+	carbon source
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
31661	24996 ChEBI	lactate	+	carbon source
31661	25017 ChEBI	leucine	+	carbon source
31661	25115 ChEBI	malate	+	carbon source
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
118893	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
118893	16301 ChEBI	nitrite	+	reduction
31661	18257 ChEBI	ornithine	+	carbon source
31661	26271 ChEBI	proline	+	carbon source
31661	17272 ChEBI	propionate	+	carbon source
31661	15361 ChEBI	pyruvate	+	carbon source
31661	17822 ChEBI	serine	+	carbon source
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
118893	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118893	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68382	esterase lipase (C 8)	-		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
118893	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
118893	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
54834	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
118893	-	+	+	-	-	+	-	-	-	-	-	-	+	-	-	-	+	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
54834	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Wastewater
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
4168	anaerobic sewage digester	Germany	DEU	Europe
54834	Anaerobic sewage digester	Germany	DEU	Europe
118893	Environment, Anaerobic sewage digester	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence U82826 (>99% sequence identity) for Castellaniella denitrificans from Microbeatlas

Aquatic Samples	3384
Soil Samples	1695
Animal Samples	1188
Plant Samples	553
Total Samples	6820

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4168	1	Risk group (German classification) According to TRBA 466
118893	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4265376v1 assembly for Castellaniella denitrificans CCUG 39541	scaffold	GCA_042653765	56119	69.21

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4168	Castellaniella denitrificans strain NKNTAU 16S ribosomal RNA gene, complete sequence	U82826	1470		56119

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Castellaniella hirudinis sp. nov., isolated from the skin of Hirudo verbana.	Glaeser SP, Galatis H, Martin K, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.041392-0	2012
Phylogeny	Castellaniella daejeonensis sp. nov., isolated from soil.	Lee M, Jung HM, Woo SG, Yoo SA, Ten LN	Int J Syst Evol Microbiol	10.1099/ijs.0.018887-0	2009
Phylogeny	Castellaniella ginsengisoli sp. nov., a beta-glucosidase-producing bacterium.	Kim MK, Srinivasan S, Kim YJ, Yang DC	Int J Syst Evol Microbiol	10.1099/ijs.0.008300-0	2009
Phylogeny	Castellaniella caeni sp. nov., a denitrifying bacterium isolated from sludge of a leachate treatment plant.	Liu QM, Ten LN, Im WT, Lee ST	Int J Syst Evol Microbiol	10.1099/ijs.0.65314-0	2008
Phylogeny	Castellaniella gen. nov., to accommodate the phylogenetic lineage of Alcaligenes defragrans, and proposal of Castellaniella defragrans gen. nov., comb. nov. and Castellaniella denitrificans sp. nov.	Kampfer P, Denger K, Cook AM, Lee ST, Jackel U, Denner EBM, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63989-0	2006

References

#4168	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11046
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27942	IJSEM 815 2006 ( DOI 10.1099/ijs.0.63989-0 , PubMed 16585701 )
#31661	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27942
#37484	; Curators of the CIP;
#54834	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 39541
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118893	Collection of Institut Pasteur ; Curators of the CIP; CIP 109221
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Castellaniella denitrificans (CIP 109221, DSM 11046, CCUG 39541)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042653765

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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