Name: Castellaniella defragrans 51 Men
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 345

Type strain:

Species: Castellaniella defragrans

Strain Designation: 51 Men

Culture col. no.: DSM 12144, CCUG 39789, LMG 18537, CIP 105605

Strain history: CIP <- 1998, J. Harder, Max Planck Inst., Bremen, Germany: strain 51Men, Alcaligenes defragrans

NCBI tax ID(s): 75697 (species)

SI-ID 12162

LMG 18537, CCUG 39789, DSM 12144, CIP 105605

Other strains from Castellaniella defragrans

C. defragrans 54Pin, 54 Pin, DSM 12141, CCUG ... (type strain)
C. defragrans 65Phen, CCUG 39792, DSM 12143, ...
C. defragrans 62Car, DSM 12142, CCUG 39791, CIP ...

Section

Castellaniella defragrans 51 Men is an aerobe, mesophilic, Gram-negative bacterium that was isolated from activated sludge.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Alcaligenaceae*
	Genus	*Castellaniella*
	Species	**Castellaniella defragrans**
	Full Scientific Name (LPSN)	Castellaniella defragrans (Foss et al. 1998) Kämpfer et al. 2006

Synonym

Alcaligenes defragrans

Information on morphological and physiological properties Morphology

[Ref.: #118875]	Gram stain	negative

[Ref.: #118875]	Cell shape	rod-shaped

[Ref.: #118875]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4554]

Culture medium

ANAEROBIC THAUERA MEDIUM (DSMZ Medium 1315)

[Ref.: #4554]

Culture medium growth

[Ref.: #4554]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4554]

Culture medium composition

expand / minimize

[Ref.: #4554]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #4554]

Culture medium growth

[Ref.: #4554]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4554]

Culture medium composition

expand / minimize

[Ref.: #38096]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #38096]

Culture medium growth

[Ref.: #38096]

Culture medium composition

expand / minimize

[Ref.: #118875]

Culture medium

CIP Medium 3

[Ref.: #118875]

Culture medium growth

[Ref.: #118875]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4554]		growth	30 °C
[Ref.: #38096]		growth	30 °C
[Ref.: #54932]		growth	25 °C
[Ref.: #118875]		growth	15-41 °C
[Ref.: #118875]	no	growth	5 °C
[Ref.: #118875]	no	growth	45 °C

[Ref.: #4554]	Temperature range	mesophilic
[Ref.: #38096]	Temperature range	mesophilic
[Ref.: #54932]	Temperature range	mesophilic
[Ref.: #118875]	Temperature range	psychrophilic
[Ref.: #118875]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #54932]	Oxygen tolerance	aerobe
[Ref.: #118875]	Oxygen tolerance	obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #118875]	NaCl		growth	0-4	%
[Ref.: #118875]	NaCl	no	growth	6	%
[Ref.: #118875]	NaCl	no	growth	8	%
[Ref.: #118875]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #118875]	citrate ChEBI	+	carbon source
[Ref.: #118875]	esculin ChEBI	-	hydrolysis
[Ref.: #118875]	hippurate ChEBI	+	hydrolysis
[Ref.: #118875]	malonate ChEBI	-	assimilation
[Ref.: #118875]	nitrate ChEBI	+	builds gas from
[Ref.: #118875]	nitrate ChEBI	+	reduction
[Ref.: #118875]	nitrate ChEBI	+	respiration
[Ref.: #118875]	nitrite ChEBI	+	builds gas from
[Ref.: #118875]	nitrite ChEBI	+	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #118875]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #118875]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #118875]		acetoin ChEBI	-
[Ref.: #118875]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118875]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #118875]	amylase	-
[Ref.: #118875]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118875]	caseinase	-	3.4.21.50
[Ref.: #118875]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #118875]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118875]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #118875]	gelatinase	+
[Ref.: #118875]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #118875]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118875]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118875]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118875]	oxidase	+
[Ref.: #118875]	phenylalanine ammonia-lyase	+	4.3.1.24
[Ref.: #118875]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118875]	tryptophan deaminase	-
[Ref.: #118875]	tween esterase	-
[Ref.: #118875]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #118875]	1242	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	+	+	+	+	-	+	-	+	+	+	+	+	-	+	+	-	-	+	-	-	+	-	+	-	-	-	+	+	-	-	+	-	-	-	-	+	+	+	+	+	+	+	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118875]	8836	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4554]	Sample type/isolated from	activated sludge
[Ref.: #4554]	Country	Germany
[Ref.: #4554]	Country ISO 3 Code	DEU
[Ref.: #4554]	Continent	Europe

[Ref.: #54932]	Sample type/isolated from	Activated sludge
[Ref.: #54932]	Sampling date	1995
[Ref.: #54932]	Country	Germany
[Ref.: #54932]	Country ISO 3 Code	DEU
[Ref.: #54932]	Continent	Europe

[Ref.: #118875]	Sample type/isolated from	Environment, Activated sludge
[Ref.: #118875]	Geographic location (country and/or sea, region)	Northern
[Ref.: #118875]	Country	Germany
[Ref.: #118875]	Country ISO 3 Code	DEU
[Ref.: #118875]	Continent	Europe
[Ref.: #118875]	Isolation date	1995
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4554]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118875]	Biosafety level	1	Risk group (French classification)

Availability in culture collections External links

[Ref.: #4554]

Culture collection no.

DSM 12144, CCUG 39789, LMG 18537, CIP 105605

[Ref.: #70025]

SI-ID 12162

References

#4554

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12144

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#38096 ; Curators of the CIP;
#54932 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 39789

#68382

Automatically annotated from API zym .

#70025

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12162.1 )

#118875 Collection of Institut Pasteur ; Curators of the CIP; CIP 105605
* These data were automatically processed and therefore are not curated

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