Alcaligenes faecalis subsp. phenolicus (DSM 16503, CCUG 54936, NRRL B-41076)

Name: Alcaligenes faecalis subsp. phenolicus (DSM 16503, CCUG 54936, NRRL B-41076)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 338

Type strain

Strain Designation J

Culture col. no. DSM 16503 CCUG 54936 NRRL B-41076 J CIP 110691

NCBI tax ID(s) 1120962 232846

Special Collections DSMZ CCUG CIP

Links

Alcaligenes faecalis subsp. phenolicus J is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from wastewater bioprocessor.

Gram-negative rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 215486

DSM 16503,NRRL B-41076,CCUG 54936,CIP 110691

@ref 20215

Last LPSN update 2025-12-16 09:46:50

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Alcaligenaceae

Genus Alcaligenes

Species Alcaligenes faecalis subsp. phenolicus

Full scientific name Alcaligenes faecalis subsp. phenolicus Rehfuss and Urban 2006

Synonyms (1)

Alcaligenes phenolicus

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
119163	negative	rod-shaped
125438	negative		96.729
125439	negative		99.5

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6380	REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)	Medium recipe at MediaDive	Name: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
6380	MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)	Medium recipe at MediaDive	Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457; with strain-specific modifications) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l Phenol 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
36444	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119163	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6380	positive	growth	30	mesophilic
36444	positive	growth	30	mesophilic
60397	positive	growth	30-37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
60397	aerobe
125439	obligate aerobe	91.7

Spore formation

@ref	Spore formation	Confidence
125439		99.7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	gelatinase	-		from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
6380	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	+	+	+
6380	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Bioreactor	-
#Engineered	#Waste	#Wastewater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6380	wastewater bioprocessor	Texas, Houston	USA	USA	North America
60397	Wastewater bioprocessor	Texas	USA	USA	North America
119163	Environment, Wastewater bioprocessor	Texas	United States of America	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6380	1	Risk group (German classification) According to TRBA 466
119163	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
6380	ASM42938v1 assembly for Alcaligenes phenolicus DSM 16503	scaffold	GCA_000429385	1120962.3	2524023144	1120962	72.81
66792	ASM2634410v1 assembly for Alcaligenes phenolicus DSM 16503	contig	GCA_026344105	232846.19	8118925266	232846	70.47

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
6380	Alcaligenes sp. J 16S ribosomal RNA gene, partial sequence	AY296718	1503	232846
124043	Alcaligenes faecalis subsp. phenolicus strain DSM 16503 16S ribosomal RNA gene, partial sequence.	ON597429	1443	232846
6380	Alcaligenes phenolicus DSM 16503 G456DRAFT_scaffold00024.24_C, whole genome shotgun sequence	AUBT01000026	1849	1120962

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Alcaligenes faecalis subsp. phenolicus DSM 16503
NCBI: GCA_026344105

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.70	no
125439	motility	BacteriaNetⓘ	yes	89.40	no
125439	gram_stain	BacteriaNetⓘ	negative	99.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	91.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alcaligenes faecalis subsp. phenolicus DSM 16503
PATRIC: 1120962.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	96.73	no
125438	anaerobic	anaerobicⓘ	no	96.77	no
125438	spore-forming	spore-formingⓘ	no	85.63	no
125438	aerobic	aerobicⓘ	yes	85.92	yes
125438	thermophilic	thermophileⓘ	no	98.40	yes
125438	flagellated	motile2+ⓘ	yes	81.39	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Heterologous Expression and Characterization of A Novel Ochratoxin A Degrading Enzyme, N-acyl-L-amino Acid Amidohydrolase, from Alcaligenes faecalis.	Zhang H, Zhang Y, Yin T, Wang J, Zhang X.	Toxins (Basel)	10.3390/toxins11090518	2019
Pathogenicity	A Newly Isolated Alcaligenes faecalis ANSA176 with the Capability of Alleviating Immune Injury and Inflammation through Efficiently Degrading Ochratoxin A.	Zheng R, Qing H, Ma Q, Huo X, Huang S, Zhao L, Zhang J, Ji C.	Toxins (Basel)	10.3390/toxins14080569	2022
	Genome sequencing and analysis of Alcaligenes faecalis subsp. phenolicus MB207.	Basharat Z, Yasmin A, He T, Tong Y.	Sci Rep	10.1038/s41598-018-21919-4	2018
Genetics	Complete genome sequence and analysis of Alcaligenes faecalis strain Mc250, a new potential plant bioinoculant.	Felestrino EB, Sanchez AB, Caneschi WL, Lemes CGC, Assis RAB, Cordeiro IF, Fonseca NP, Villa MM, Vieira IT, Kamino LHY, do Carmo FF, da Silva AM, Thomas AM, Patane JSL, Ferreira FC, de Freitas LG, Varani AM, Ferro JA, Silva RS, Almeida NF, Garcia CCM, Setubal JC, Moreira LM.	PLoS One	10.1371/journal.pone.0241546	2020
Enzymology	Bioenzymatic detoxification of mycotoxins.	Liu M, Zhang X, Luan H, Zhang Y, Xu W, Feng W, Song P.	Front Microbiol	10.3389/fmicb.2024.1434987	2024
Biotechnology	Comparative Genomics Reveals Novel Species and Insights into the Biotechnological Potential, Virulence, and Resistance of Alcaligenes.	Pedrosa-Silva F, Venancio TM.	Genes (Basel)	10.3390/genes14091783	2023
	Mechanisms by which microbial enzymes degrade four mycotoxins and application in animal production: A review.	Sun H, He Z, Xiong D, Long M.	Anim Nutr	10.1016/j.aninu.2023.09.003	2023
Pathogenicity	A new and promiscuous alpha/beta hydrolase from Acinetobacter tandoii DSM 14970^T inactivates the mycotoxin ochratoxin A.	Sanchez-Arroyo A, Plaza-Vinuesa L, Abeijon-Mukdsi MC, de Las Rivas B, Mancheno JM, Munoz R.	Appl Microbiol Biotechnol	10.1007/s00253-024-13073-x	2024
	Sulphonated azo dye decolorization by Alcaligenes faecalis subsp. phenolicus MB207: Insights from laboratory and computational analysis.	Basharat Z, Yasmin A.	Biophys Chem	10.1016/j.bpc.2022.106806	2022
Phylogeny	Alcaligenes faecalis subsp. phenolicus subsp. nov. a phenol-degrading, denitrifying bacterium isolated from a graywater bioprocessor.	Rehfuss M, Urban J	Syst Appl Microbiol	10.1016/j.syapm.2005.03.003	2005

References

#6380	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16503
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#36444	; Curators of the CIP;
#60397	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54936
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#119163	Collection of Institut Pasteur ; Curators of the CIP; CIP 110691
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alcaligenes faecalis subsp. phenolicus (DSM 16503, CCUG 54936, NRRL B-41076)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000429385

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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